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However, the use of glycosyltransferases (GTs) for their synthesis is constrained by stringent sugar nucleotide donor selectivity, which limits the structural diversity of accessible glycosides. The chimeric NB1\/\/CC1, constituted of 2 self-assembled domains originating from plant glycosyltransferases, was previously demonstrated to transfer glucose onto a wider range of natural compounds than its parental enzymes. This study demonstrates that this chimeric enzyme also exhibits a relaxed selectivity toward sugar nucleotide donors. This donor promiscuity can further be enhanced by the Mg2+ cation, which increases the sugar transfer rate for the chimeric enzyme. NB1\/\/CC1 can therefore be used as a single biocatalyst to generate a library of glycosidic variants of a given natural glycosylated compound.<\/jats:p>","DOI":"10.1021\/acs.jafc.6c10592","type":"journal-article","created":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T01:03:26Z","timestamp":1788397406000},"page":"27840-27849","source":"Crossref","is-referenced-by-count":0,"title":["Domain-Swapping\nof a GT-B Glycosyltransferase\nBroadens Its Sugar Donor Range for Glycodiversification of Natural\nProducts"],"prefix":"10.1021","volume":"74","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-0461-3387","authenticated-orcid":true,"given":"Caroline","family":"Gilleron","sequence":"first","affiliation":[{"name":"Institut de Chimie Organique et Analytique , , ,","place":["Orle\u0301ans, France, F-45067"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arnaud","family":"Pa\u0302ris","sequence":"additional","affiliation":[{"name":"Institut de Chimie Organique et Analytique , , ,","place":["Orle\u0301ans, France, F-45067"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ilham","family":"Alloui","sequence":"additional","affiliation":[{"name":"Institut de Chimie Organique et Analytique , , ,","place":["Orle\u0301ans, France, F-45067"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tiffany","family":"Rundstadler","sequence":"additional","affiliation":[{"name":"Institut de Chimie Organique et Analytique , , ,","place":["Orle\u0301ans, France, F-45067"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lae\u0308titia","family":"Fouge\u0300re","sequence":"additional","affiliation":[{"name":"Institut de Chimie Organique et Analytique , , ,","place":["Orle\u0301ans, France, F-45067"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Malika","family":"Mekhalfi","sequence":"additional","affiliation":[{"name":"Institut de Chimie Organique et Analytique , , ,","place":["Orle\u0301ans, France, F-45067"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1637-4966","authenticated-orcid":true,"given":"Pierre","family":"Lafite","sequence":"additional","affiliation":[{"name":"Institut de Chimie Organique et Analytique , , ,","place":["Orle\u0301ans, France, F-45067"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,8,31]]},"reference":[{"issue":"1","key":"2026090917081770000_cit1","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1093\/glycob\/cww086","article-title":"Biological\nRoles of Glycans","volume":"27","author":"Varki","year":"2017","journal-title":"Glycobiology"},{"issue":"11","key":"2026090917081770000_cit2","doi-asserted-by":"publisher","first-page":"542","DOI":"10.1016\/j.tplants.2005.09.007","article-title":"Plant Secondary\nMetabolism Glycosyltransferases: The Emerging Functional Analysis","volume":"10","author":"Gachon","year":"2005","journal-title":"Trends Plant Sci."},{"issue":"15","key":"2026090917081770000_cit3","doi-asserted-by":"publisher","first-page":"3321","DOI":"10.1021\/jf405570u","article-title":"Substituent\nEffects on in Vitro Antioxidizing\nProperties, Stability, and Solubility in Flavonoids","volume":"62","author":"Plaza","year":"2014","journal-title":"J. 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