{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T22:17:33Z","timestamp":1788387453460,"version":"build-2803163510"},"reference-count":37,"publisher":"American Chemical Society (ACS)","issue":"28","license":[{"start":{"date-parts":[[2018,6,21]],"date-time":"2018-06-21T00:00:00Z","timestamp":1529539200000},"content-version":"vor","delay-in-days":365,"URL":"https:\/\/pubs.acs.org\/page\/policy\/authorchoice_termsofuse.html"}],"funder":[{"DOI":"10.13039\/100005243","name":"United Nations Educational, Scientific and Cultural Organization","doi-asserted-by":"publisher","award":["NA"],"award-info":[{"award-number":["NA"]}],"id":[{"id":"10.13039\/100005243","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["NA"],"award-info":[{"award-number":["NA"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004409","name":"Minist\u00e8re de l\u2019Enseignement Sup\u00e9rieur, de la Recherche Scientifique et des Technologies de l'Information et de la Communication","doi-asserted-by":"publisher","award":["TK\/08\/2012"],"award-info":[{"award-number":["TK\/08\/2012"]}],"id":[{"id":"10.13039\/501100004409","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2017,7,19]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The aim of this study was to compare immunogenic and toxic gluten peptides related to celiac disease (CD). 100 accessions of genotypes selected during the 20th century in Tunisia were in vitro digested and then analyzed by UPLC\/ESI-MS technique using an isotopically labeled internal standard. The first MANOVA confirmed a high variability in the content of immunogenic and toxic peptides reflecting high genetic diversity in the germplasm released during the past century in Tunisia, consistently with PCA and clustering analysis results. Our finding showed also important variability in CD epitopes due to growing season\u2019s climate scenarios. Moreover, the second MANOVA revealed significant differences between abandoned and modern cultivars\u2019 CD-related peptide amounts. Although we could not conclude that there was an augment of allergens in newly selected durum wheat lines compared to abandoned ones, we demonstrated that modern genotype peptides were less sensitive to climate variation, which is a useful indicator for wheat breeders.<\/jats:p>","DOI":"10.1021\/acs.jafc.7b02041","type":"journal-article","created":{"date-parts":[[2017,6,21]],"date-time":"2017-06-21T11:25:55Z","timestamp":1498044355000},"page":"5837-5846","source":"Crossref","is-referenced-by-count":16,"title":["Understanding the Effects of Genotype, Growing Year,\nand Breeding on Tunisian Durum Wheat Allergenicity. 2. The Celiac\nDisease Case"],"prefix":"10.1021","volume":"65","author":[{"given":"Fatma","family":"Boukid","sequence":"first","affiliation":[{"name":"University of Sfax , , B.P 1177 , ,","place":["Sfax, Tunisia, 3018"]},{"name":"University of Parma , , Parco Area delle Scienze 27\/A , ,","place":["Parma, Italy, 43124"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4444-0676","authenticated-orcid":true,"given":"Barbara","family":"Prandi","sequence":"additional","affiliation":[{"name":"University of Parma , , Parco Area delle Scienze 27\/A , ,","place":["Parma, Italy, 43124"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefano","family":"Sforza","sequence":"additional","affiliation":[{"name":"University of Parma , , Parco Area delle Scienze 27\/A , ,","place":["Parma, Italy, 43124"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rhouma","family":"Sayar","sequence":"additional","affiliation":[{"name":"Tunisian Higher School of Agriculture of Kef Boulifa , ,","place":["Kef, Tunisia, 7119"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong Weon","family":"Seo","sequence":"additional","affiliation":[{"name":"Korea University , , Anam-Dong, Seongbuk-Gu, ,","place":["Seoul, Republic of Korea, 136-713"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mondher","family":"Mejri","sequence":"additional","affiliation":[{"name":"University of Sfax , , B.P 1177 , ,","place":["Sfax, Tunisia, 3018"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ines","family":"Yacoubi","sequence":"additional","affiliation":[{"name":"University of Sfax , , B.P 1177 , ,","place":["Sfax, Tunisia, 3018"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2017,6,21]]},"reference":[{"key":"2026090218035363300_cit1","doi-asserted-by":"publisher","first-page":"559","DOI":"10.1111\/j.1365-2036.2006.02768.x","article-title":"Review article: Safe\namounts of gluten for patients with wheat allergy or coeliac disease","volume":"23","author":"Hischenhuber","year":"2006","journal-title":"Aliment. 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Chem."},{"key":"2026090218035363300_cit30","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1007\/BF00806090","article-title":"Coeliac\ndisease: a review of the causative agents and their possibly mechanisms\nof action","volume":"10","author":"Cornell","year":"1996","journal-title":"Amino Acids"},{"key":"2026090218035363300_cit31","doi-asserted-by":"publisher","first-page":"837416","DOI":"10.1100\/2012\/837416","article-title":"Are Ancient\nDurum Wheats Less Toxic to Celiac Patients?\nA Study of \u03b1-Gliadin from Graziella Ra and Kamut","volume":"2012","author":"Colomba","year":"2012","journal-title":"Sci. World J."},{"key":"2026090218035363300_cit32","doi-asserted-by":"publisher","first-page":"43","DOI":"10.1007\/s00726-006-0420-0","article-title":"A unified hypothesis of coeliac disease with implications\nfor management of patients","volume":"33","author":"Cornell","year":"2007","journal-title":"Amino Acids"},{"key":"2026090218035363300_cit33","doi-asserted-by":"publisher","first-page":"223","DOI":"10.1016\/j.jcs.2012.04.008","article-title":"Composition of peptide\nmixtures derived from simulated gastrointestinal digestion of prolamins\nfrom different wheat varieties","volume":"56","author":"Prandi","year":"2012","journal-title":"J. Cereal Sci."},{"key":"2026090218035363300_cit34","doi-asserted-by":"publisher","first-page":"133","DOI":"10.1016\/S0733-5210(03)00030-4","article-title":"Molecular\nand biochemical impacts of environmental\nfactors on wheat grain development and protein synthesis","volume":"38","author":"DuPont","year":"2003","journal-title":"J. Cereal Sci."},{"key":"2026090218035363300_cit35","article-title":"Anti-Wheat\nFad Diets Undermine Global Food Security Efforts: Wheat consumption\nhealthy despite claims in self-help publications","author":"Pen\u0303a","year":"2014","journal-title":"CIMMYT Wheat Discuss. Pap."},{"key":"2026090218035363300_cit36","doi-asserted-by":"publisher","first-page":"1043","DOI":"10.1016\/j.clnu.2013.02.003","article-title":"Evaluation\nof the safety of ancient strains of wheat in coeliac disease reveals\nheterogeneous small intestinal T cell responses suggestive of coeliac\ntoxicity","volume":"32","author":"S\u030culigoj","year":"2013","journal-title":"Clin. Nutr."},{"key":"2026090218035363300_cit37","doi-asserted-by":"publisher","first-page":"1844","DOI":"10.1002\/mnfr.201500126","article-title":"Extensive in vitro gastrointestinal digestion markedly\nreduces the immune-toxicity of Triticum monococcum wheat: Implication\nfor celiac disease","volume":"59","author":"Gianfrani","year":"2015","journal-title":"Mol. Nutr. 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