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The structures of 1 and 2 were elucidated by NMR, HRMS, and single-crystal X-ray diffraction. The absolute configuration of 1 was established by comparison of calculated and experimental ECD spectra, and that of 2 was assigned by ECD comparison with 1. Compounds 1 and 2 exhibited inhibitory activity against Candida auris and C. albicans with MIC values of 3.12\u221212.5 \u03bcg\/mL, and MFC\/MIC ratios suggested a fungistatic mode of action. Both compounds also inhibited C. rugosa lipase (CRL) in a model enzyme assay, with IC50 values of 5.7 and 8.9 \u03bcM, respectively. These results identify a distinctive class of fungal steroids with antifungal activity.<\/jats:p>","DOI":"10.1021\/acs.jnatprod.6c00038","type":"journal-article","created":{"date-parts":[[2026,3,2]],"date-time":"2026-03-02T20:07:29Z","timestamp":1772482049000},"page":"2429-2435","source":"Crossref","is-referenced-by-count":1,"title":["Hexacyclic 6\/6\/6\/5\/6\/6 Spirosteroids Produced by a Marine-Derived Fungus\n                    <i>Galactomyces pseudocandidus<\/i>\n                    and Antifungal Activity against\n                    <i>Candida<\/i>\n                    Species"],"prefix":"10.1021","volume":"89","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8023-0097","authenticated-orcid":true,"given":"Hitoshi","family":"Kamauchi","sequence":"first","affiliation":[{"name":"Josai University , , 1-1 Keyaki-dai , , ,","place":["Sakado-shi, Saitama, Japan, 350-0295"]},{"name":"Musashino University , , 1-1-20, Shinmachi , , ,","place":["Nishitokyo-shi, Tokyo, Japan, 202-8585"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mikuru","family":"Kuroda","sequence":"additional","affiliation":[{"name":"Josai University , , 1-1 Keyaki-dai , , ,","place":["Sakado-shi, Saitama, Japan, 350-0295"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7226-2665","authenticated-orcid":true,"given":"Mitsuaki","family":"Suzuki","sequence":"additional","affiliation":[{"name":"Josai University , , 1-1 Keyaki-dai , , ,","place":["Sakado-shi, Saitama, Japan, 350-0295"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kanako","family":"Usui","sequence":"additional","affiliation":[{"name":"Josai University , , 1-1 Keyaki-dai , , ,","place":["Sakado-shi, Saitama, Japan, 350-0295"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuki","family":"Yatsui","sequence":"additional","affiliation":[{"name":"Josai University , , 1-1 Keyaki-dai , , ,","place":["Sakado-shi, Saitama, Japan, 350-0295"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuka","family":"Kiba","sequence":"additional","affiliation":[{"name":"Josai University , , 1-1 Keyaki-dai , , ,","place":["Sakado-shi, Saitama, Japan, 350-0295"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masashi","family":"Kitamura","sequence":"additional","affiliation":[{"name":"Josai University , , 1-1 Keyaki-dai , , ,","place":["Sakado-shi, Saitama, Japan, 350-0295"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshiaki","family":"Sugita","sequence":"additional","affiliation":[{"name":"Josai University , , 1-1 Keyaki-dai , , ,","place":["Sakado-shi, Saitama, Japan, 350-0295"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,3,2]]},"reference":[{"key":"2026100909065582500_cit1","doi-asserted-by":"publisher","first-page":"41","DOI":"10.1111\/j.1348-0421.2008.00083.x","article-title":"Candida auris sp. nov., a novel ascomycetous yeast isolated from the external ear canal of an inpatient in a Japanese hospital","volume":"53","author":"Satoh","year":"2009","journal-title":"Microbiol. 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