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Despite the limited fungal material available, the compound was isolated and characterized as a homodetic cyclic hexapeptide by NMR and MS\/MS analyses, and its structure was confirmed by high-efficiency solid-phase peptide synthesis. Recently implemented genome mining further validated the structure and provided a broader insight into the cyclopeptide-producing capabilities of this rare mushroom. Named viduamanide, this compound is a non-canonical cycloamanide of the \u201cprolineless\u201d subtype and the first of its kind ever reported to not contain a proline residue.<\/jats:p>","DOI":"10.1021\/acs.jnatprod.6c00782","type":"journal-article","created":{"date-parts":[[2026,8,12]],"date-time":"2026-08-12T16:04:29Z","timestamp":1786550669000},"page":"2741-2749","source":"Crossref","is-referenced-by-count":0,"title":["Genome Mining and Total Synthesis of Viduamanide, A Non-canonical Prolineless Cycloamanide from\n                    <i>Amanita vidua<\/i>"],"prefix":"10.1021","volume":"89","author":[{"given":"Adrien","family":"Jagora","sequence":"first","affiliation":[{"name":"Universite\u0301 Paris-Saclay, CNRS, BioCIS, 17 Avenue des Sciences , ,","place":["Orsay, France, 91400"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Charpentier","sequence":"additional","affiliation":[{"name":"Universite\u0301 Paris-Saclay, CNRS, BioCIS, 17 Avenue des Sciences , ,","place":["Orsay, France, 91400"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-2964-4766","authenticated-orcid":true,"given":"Tom","family":"Lorthios","sequence":"additional","affiliation":[{"name":"INSERM U1338, Sorbonne Universite\u0301 , ,","place":["Paris, France, 75005"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pablo","family":"Tris","sequence":"additional","affiliation":[{"name":"Universite\u0301 Paris-Saclay, CNRS, BioCIS, 17 Avenue des Sciences , ,","place":["Orsay, France, 91400"]},{"name":"INSERM U1338, Sorbonne Universite\u0301 , ,","place":["Paris, France, 75005"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pierre-Arthur","family":"Moreau","sequence":"additional","affiliation":[{"name":"Faculte\u0301 de Pharmacie, Universite\u0301 Lille , 3 rue du Pr Laguesse , ,","place":["Lille, France, F-59000"]},{"name":"EA 4489 LGCgE, Universite\u0301 Lille , ,","place":["Lille, France, F-59000"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Michel","family":"Bellanger","sequence":"additional","affiliation":[{"name":"CEFE, CNRS, Univ Montpellier, EPHE, IRD, INSERM , ,","place":["Montpellier, France, 34293"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Franc\u0327ois","family":"Gallard","sequence":"additional","affiliation":[{"name":"Institut de Chimie des Substances Naturelles, CNRS, ICSN UPR 2301, Universite\u0301 Paris-Saclay , ,","place":["Gif-sur-Yvette, France, 91198"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Re\u0301mi","family":"Franco","sequence":"additional","affiliation":[{"name":"Universite\u0301 Paris-Saclay, CNRS, BioCIS, 17 Avenue des Sciences , ,","place":["Orsay, France, 91400"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Blandine","family":"Se\u0301on-Me\u0301niel","sequence":"additional","affiliation":[{"name":"Universite\u0301 Paris-Saclay, CNRS, BioCIS, 17 Avenue des Sciences , ,","place":["Orsay, France, 91400"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonia","family":"Gasch Illescas","sequence":"additional","affiliation":[{"name":"Universidad de Sevilla , , San Fernando 4 , ,","place":["Sevilla, Spain, E-41004"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2197-3843","authenticated-orcid":true,"given":"Thierry","family":"Milcent","sequence":"additional","affiliation":[{"name":"Universite\u0301 Paris-Saclay, CNRS, BioCIS, 17 Avenue des Sciences , ,","place":["Orsay, France, 91400"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2153-4290","authenticated-orcid":true,"given":"Mehdi A.","family":"Beniddir","sequence":"additional","affiliation":[{"name":"Universite\u0301 Paris-Saclay, CNRS, BioCIS, 17 Avenue des Sciences , ,","place":["Orsay, France, 91400"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3351-4708","authenticated-orcid":true,"given":"Pierre","family":"Le Pogam","sequence":"additional","affiliation":[{"name":"Universite\u0301 Paris-Saclay, CNRS, BioCIS, 17 Avenue des Sciences , ,","place":["Orsay, France, 91400"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,8,12]]},"reference":[{"issue":"48","key":"2026100909075718100_cit1","doi-asserted-by":"publisher","first-page":"19097","DOI":"10.1073\/pnas.0707340104","article-title":"Gene Family Encoding the Major Toxins of Lethal Amanita Mushrooms","volume":"104","author":"Hallen","year":"2007","journal-title":"Proc. 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