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Assays to measure plant HDAC activity were optimized by testing a number of HDAC substrates. The activity of romidepsin was greater when its macrocyclic-forming disulfide bridge was reduced to liberate a highly reactive free butenyl thiol side chain. Reduction was achieved using 200 mM tris\u00ad(2-carboxyethyl)\u00adphosphine hydrochloride. A similar bioactivation of the proherbicide via reduction of the disulfide bridge of romidepsin was observed in plant-cell-free extracts. Molecular dynamic simulation of the binding of romidepsin to Arabidopsis thaliana HDAC19 indicated the reduced form of the compound could reach deep inside the catalytic domain and interact with an associated zinc atom required for enzyme activity.<\/jats:p>","DOI":"10.1021\/acs.jnatprod.9b00405","type":"journal-article","created":{"date-parts":[[2020,2,24]],"date-time":"2020-02-24T10:46:42Z","timestamp":1582541202000},"page":"843-851","source":"Crossref","is-referenced-by-count":20,"title":["The Contribution of Romidepsin to the Herbicidal Activity\nof\n                    <i>Burkholderia rinojensis<\/i>\n                    Biopesticide"],"prefix":"10.1021","volume":"83","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0745-7866","authenticated-orcid":true,"given":"Daniel\nK.","family":"Owens","sequence":"first","affiliation":[{"name":"University of Hawaii at Manoa , , , ,","place":["Honolulu, Hawaii, United States, 96822"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joanna","family":"Bajsa-Hirschel","sequence":"additional","affiliation":[{"name":"USDA-ARS, Thad Cochran Center , , University Avenue , ,","place":["University, Mississippi, United States, 38677"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7210-5168","authenticated-orcid":true,"given":"Stephen O.","family":"Duke","sequence":"additional","affiliation":[{"name":"USDA-ARS, Thad Cochran Center , , University Avenue , ,","place":["University, Mississippi, United States, 38677"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0383-2529","authenticated-orcid":true,"given":"Caio A.","family":"Carbonari","sequence":"additional","affiliation":[{"name":"Sa\u0303o Paulo State University , , , , ,","place":["Botucatu, SP, Brazil, 01049-010"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7688-4461","authenticated-orcid":true,"given":"Giovanna L. 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