{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T14:08:15Z","timestamp":1789740495077,"version":"4.0.1"},"reference-count":32,"publisher":"American Chemical Society (ACS)","issue":"37","license":[{"start":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T00:00:00Z","timestamp":1788393600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T00:00:00Z","timestamp":1788393600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T00:00:00Z","timestamp":1788393600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"funder":[{"DOI":"10.13039\/100006483","name":"AbbVie","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100006483","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,9,18]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>In this work, we present the creation and implementation of a ChemBead-enabled, on-DNA high-throughput experimentation (HTE) platform to identify more general and mild reaction conditions for challenging C\u2013N couplings. The platform was developed by adapting our small-molecule system to address the inherent challenges of on-DNA chemistry. The results generated by the platform were compared to the standard operating procedure for DNA-encoded library (DEL) production to ensure the translation of any promising conditions. The platform was used to discover a condition that has not been previously reported for on-DNA C\u2013N coupling. This condition is best suited for secondary amines and select primary amines on short DNA substrates; primary aliphatic amines on elongated DNA-tagged aryl halide conjugates remain a challenge. This newly identified condition can be applied to DNA-encoded library constructions.<\/jats:p>","DOI":"10.1021\/acs.joc.6c00134","type":"journal-article","created":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T21:48:01Z","timestamp":1788472081000},"page":"12471-12480","source":"Crossref","is-referenced-by-count":0,"title":["The Development\nand\nApplication of a ChemBead-Enabled\nOn-DNA HTE Platform for Challenging C\u2013N Couplings"],"prefix":"10.1021","volume":"91","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4953-6768","authenticated-orcid":true,"given":"Ana L.","family":"Aguirre","sequence":"first","affiliation":[{"name":"AbbVie, Inc. , 1 N Waukegan Rd , , ,","place":["North Chicago, Illinois, United States, 60064"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9596-263X","authenticated-orcid":true,"given":"Amanda W.","family":"Dombrowski","sequence":"additional","affiliation":[{"name":"AbbVie, Inc. , 1 N Waukegan Rd , , ,","place":["North Chicago, Illinois, United States, 60064"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9164-3815","authenticated-orcid":true,"given":"Noah P.","family":"Tu","sequence":"additional","affiliation":[{"name":"AbbVie, Inc. , 1 N Waukegan Rd , , ,","place":["North Chicago, Illinois, United States, 60064"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shubhendu S.","family":"Karandikar","sequence":"additional","affiliation":[{"name":"AbbVie, Inc. , 1 N Waukegan Rd , , ,","place":["North Chicago, Illinois, United States, 60064"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9516-3483","authenticated-orcid":true,"given":"Ying","family":"Wang","sequence":"additional","affiliation":[{"name":"AbbVie, Inc. , 1 N Waukegan Rd , , ,","place":["North Chicago, Illinois, United States, 60064"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,9,3]]},"reference":[{"key":"2026091808080701500_cit1","doi-asserted-by":"publisher","first-page":"1265","DOI":"10.1021\/acsptsci.1c00118","article-title":"DNA-Encoded Chemical\nLibraries: A Comprehensive Review with Succesful Stories and Future\nChallenges","volume":"4","author":"Gironda-Martinez","year":"2021","journal-title":"ACS Pharmacol. 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