{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T14:06:32Z","timestamp":1789135592404,"version":"build-2803163510"},"reference-count":31,"publisher":"American Chemical Society (ACS)","issue":"36","license":[{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["22277088"],"award-info":[{"award-number":["22277088"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/na","name":"Haihe Laboratory of Sustainable Chemical Transformations","doi-asserted-by":"publisher","award":["25HHWCSS00026"],"award-info":[{"award-number":["25HHWCSS00026"]}],"id":[{"id":"10.13039\/na","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,9,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>S-Adenosylmethionine (SAM) is an important cofactor in a variety of biochemical reactions. In addition to serving as a versatile methyl donor, the 3-amino-3-carboxypropyl (ACP) group of SAM is also involved in the biosynthesis of many important natural products including antibiotics and signaling molecules. We developed four SAM-based probes for labeling the substrates of the ACP transferases. SAM3 successfully labels the substrate of BjaI in the biosynthesis of isovaleryl homoserine lactone (IV-AHL), a quorum-sensing signaling molecule. SAM1 is capable of labeling the substrate of CntL in staphylopine biosynthesis with both pure compounds and complex metabolites. Therefore, they are promising tools for labeling and identifying the substrates of ACP transferases.<\/jats:p>","DOI":"10.1021\/acs.joc.6c00987","type":"journal-article","created":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T10:50:28Z","timestamp":1788432628000},"page":"12445-12450","source":"Crossref","is-referenced-by-count":0,"title":["Design and Synthesis\nof SAM Analogues for Labeling\nSubstrates of ACP Transferases"],"prefix":"10.1021","volume":"91","author":[{"given":"Xiangyu","family":"Wu","sequence":"first","affiliation":[{"name":"Tianjin University , , ,","place":["Tianjin, China, 300072"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6655-2818","authenticated-orcid":true,"given":"Min","family":"Dong","sequence":"additional","affiliation":[{"name":"Tianjin University , , ,","place":["Tianjin, China, 300072"]},{"name":"Haihe Laboratory of Sustainable Chemical Transformations , ,","place":["Tianjin, China, 300192"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,9,1]]},"reference":[{"issue":"3","key":"2026091109113411200_cit1","doi-asserted-by":"publisher","DOI":"10.1002\/biot.202300650","article-title":"Enhanced\nsynthesis of S-adenosyl-L-methionine through combinatorial metabolic\nengineering and Bayesian optimization in Saccharomyces cerevisiae","volume":"19","author":"Xiao","year":"2024","journal-title":"Biotechnol. J."},{"issue":"11","key":"2026091109113411200_cit2a","doi-asserted-by":"publisher","first-page":"2611","DOI":"10.1021\/acs.accounts.8b00356","article-title":"Mechanism\nof Radical Initiation in the Radical S-Adenosyl-l-methionine Superfamily","volume":"51","author":"Broderick","year":"2018","journal-title":"Acc. Chem. Res."},{"issue":"6","key":"2026091109113411200_cit2b","doi-asserted-by":"publisher","first-page":"769","DOI":"10.1007\/s00775-019-01706-w","article-title":"Radical SAM enzymes: surprises along the path to understanding\nmechanism","volume":"24","author":"Broderick","year":"2019","journal-title":"J. Biol. Inorg. Chem."},{"issue":"24","key":"2026091109113411200_cit2c","doi-asserted-by":"publisher","first-page":"3161","DOI":"10.1021\/acs.biochem.4c00518","article-title":"Initiation, Propagation,\nand Termination in the Chemistry of Radical SAM Enzymes","volume":"63","author":"Ruszczycky","year":"2024","journal-title":"Biochemistry"},{"issue":"9","key":"2026091109113411200_cit3a","doi-asserted-by":"publisher","first-page":"1521","DOI":"10.1039\/D2NP00086E","article-title":"S-Adenosylmethionine:\nmore than just a methyl donor","volume":"40","author":"Lee","year":"2023","journal-title":"Nat. Prod. Rep."},{"issue":"4","key":"2026091109113411200_cit3b","doi-asserted-by":"publisher","first-page":"356","DOI":"10.1021\/cb1003652","article-title":"Mechanism of Vibrio cholerae autoinducer-1 biosynthesis","volume":"6","author":"Wei","year":"2011","journal-title":"ACS Chem. 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Protoc."},{"issue":"11","key":"2026091109113411200_cit4b","doi-asserted-by":"publisher","first-page":"3642","DOI":"10.1021\/ja908995p","article-title":"Enzyme-catalyzed transfer of a ketone group from an\nS-adenosylmethionine analogue: a tool for the functional analysis\nof methyltransferases","volume":"132","author":"Lee","year":"2010","journal-title":"J. Am. Chem. Soc."},{"issue":"17","key":"2026091109113411200_cit4c","doi-asserted-by":"publisher","first-page":"8590","DOI":"10.3390\/ijms26178590","article-title":"Phospho-Switch: Regulation of the Activity of SAM-Dependent Methyltransferases\nUsing H-Phosphinic SAM Analogue","volume":"26","author":"Filonov","year":"2025","journal-title":"Int. J. 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