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Herein, we report a label-free assay based on isothermal titration calorimetry that provides access to observed thermodynamic and model-derived kinetic parameters associated with assembly formation under defined experimental conditions. The assay further reveals cooperative binding between amine-based nucleophiles and diols toward ortho-boronoaldehyde derivatives, providing new insight into multicomponent dynamic covalent systems.<\/jats:p>","DOI":"10.1021\/acs.joc.6c01363","type":"journal-article","created":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T11:02:24Z","timestamp":1791975744000},"page":"14068-14073","source":"Crossref","is-referenced-by-count":0,"title":["Dissecting Binary and Ternary Iminoboronate Assemblies: A Calorimetric Study of Thermodynamics, Kinetics, and Allosteric Effects"],"prefix":"10.1021","volume":"91","author":[{"given":"Joseba","family":"Ruiz","sequence":"first","affiliation":[{"name":"Certest Biotec S.L. , San Mateo de G\u00e1llego , ,","place":["Zaragoza, Spain, 50840"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1882-2558","authenticated-orcid":true,"given":"Eduardo","family":"Laga","sequence":"additional","affiliation":[{"name":"Certest Biotec S.L. , San Mateo de G\u00e1llego , ,","place":["Zaragoza, Spain, 50840"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5702-4538","authenticated-orcid":true,"given":"Adri\u00e1n","family":"Vel\u00e1zquez-Campoy","sequence":"additional","affiliation":[{"name":"Universidad de Zaragoza , , ,","place":["Zaragoza, Spain, 50018"]},{"name":"Instituto de Investigaci\u00f3n Sanitaria Arag\u00f3n (IIS Aragon) , ,","place":["Zaragoza, Spain, 50009"]},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Enfermedades Hep\u00e1ticas y Digestivas (CIBERehd) , ,","place":["Madrid, Spain, 28029"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5380-6863","authenticated-orcid":true,"given":"Jes\u00fas del","family":"Barrio","sequence":"additional","affiliation":[{"name":"Instituto de Nanociencia y Materiales de Arag\u00f3n (INMA), CSIC-Universidad de Zaragoza , ,","place":["Zaragoza, Spain, 50018"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,10,14]]},"reference":[{"issue":"7","key":"2026103005080528000_cit1","doi-asserted-by":"publisher","first-page":"4905","DOI":"10.1021\/cr200409f","article-title":"Click Chemistry for Drug Development and Diverse Chemical\u2013Biology Applications","volume":"113","author":"Thirumurugan","year":"2013","journal-title":"Chem. 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