{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T15:02:17Z","timestamp":1789657337506,"version":"4.0.1"},"reference-count":87,"publisher":"American Chemical Society (ACS)","issue":"37","license":[{"start":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T00:00:00Z","timestamp":1788480000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T00:00:00Z","timestamp":1788480000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T00:00:00Z","timestamp":1788480000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"funder":[{"DOI":"10.13039\/100021109","name":"National Institute of Technology Meghalaya","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100021109","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,9,17]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>DNA, the fundamental genetic material in living organisms, is of great scientific interest due to its biological role and interactions with small molecules. Ultraviolet (UV) radiation poses a substantial environmental risk by inducing DNA damage, potentially leading to mutagenesis and skin cancers. The study investigated purpurin interaction with ct-DNA and its protective effect against UV-induced DNA damage. UV exposure caused significant DNA damage, indicated by 36% reduced absorbance; however, purpurin significantly lessened this damage to only 19%. In addition, Raman spectra showed no significant changes in purpurin-treated DNA, indicating that the DNA\u2019s native structure was preserved, suggesting that purpurin is able to protect the DNA against UV-induced DNA damage. UV\u2013vis and cyclic voltammetry studies indicated purpurin-ct-DNA complex formation with a binding constant of 104 M\u20131. Thermodynamic analysis shows negative \u0394H [-(39.91 \u00b1 0.77) kJ mol\u20131] and \u0394S [-(51.68 \u00b1 2.70) J mol\u20131], indicating hydrogen bonding and van der Waals forces, with negative \u0394G confirming spontaneity. Groove binding is the preferred binding mode as evaluated by various experimental techniques. Circular dichroism (CD) and Fourier-transform infrared (FT-IR) spectra suggested that purpurin binding does not alter DNA\u2019s structural integrity. Molecular dynamics (MD) simulation further supported the structural stability and flexibility of DNA. These findings provide mechanistic insight into the interaction of purpurin with DNA and its potential role in maintaining DNA structural integrity under UV irradiation.<\/jats:p>","DOI":"10.1021\/acs.jpcb.6c02752","type":"journal-article","created":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T11:01:58Z","timestamp":1788519718000},"page":"9362-9378","source":"Crossref","is-referenced-by-count":0,"title":["Molecular Insights\ninto the Protective Activity of\nBioactive Purpurin against UV-Induced DNA Damage: Elucidation of the\nBinding Mechanism Using Spectroscopic and Computational Studies"],"prefix":"10.1021","volume":"130","author":[{"given":"Sana","family":"Quraishi","sequence":"first","affiliation":[{"name":"National Institute of Technology Meghalaya , , Saitsohpen , , ,","place":["Sohra, Meghalaya, India, 793108"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sibasree","family":"Hojaisa","sequence":"additional","affiliation":[{"name":"Tezpur University , , ,","place":["Tezpur, India, 784028"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3173-1274","authenticated-orcid":true,"given":"Kalpana","family":"Kumari","sequence":"additional","affiliation":[{"name":"Indian Institute of Technology Guwahati , , , ,","place":["Guwahati, Assam, India, 781039"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erica W. M.","family":"Marboh","sequence":"additional","affiliation":[{"name":"North Eastern Hill University , , ,","place":["Shillong, India, 793022"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kripamoy","family":"Aguan","sequence":"additional","affiliation":[{"name":"North Eastern Hill University , , ,","place":["Shillong, India, 793022"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anupam Nath","family":"Jha","sequence":"additional","affiliation":[{"name":"Tezpur University , , ,","place":["Tezpur, India, 784028"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1902-403X","authenticated-orcid":true,"given":"Atanu","family":"Singha Roy","sequence":"additional","affiliation":[{"name":"National Institute of Technology Meghalaya , , Saitsohpen , , ,","place":["Sohra, Meghalaya, India, 793108"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,9,4]]},"reference":[{"issue":"12","key":"2026091708472505400_cit1","doi-asserted-by":"publisher","first-page":"2379","DOI":"10.1093\/carcin\/18.12.2379","article-title":"Induction\nof Oxidative DNA Base Damage in Human Skin Cells by UV and near Visible\nRadiation","volume":"18","author":"Kvam","year":"1997","journal-title":"Carcinogenesis"},{"issue":"3","key":"2026091708472505400_cit2","doi-asserted-by":"publisher","first-page":"99","DOI":"10.1007\/s42764-020-00009-8","article-title":"Mechanisms\nof UV-Induced Mutations and Skin Cancer","volume":"1","author":"Pfeifer","year":"2020","journal-title":"Genome\nInstab. 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