{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T13:44:44Z","timestamp":1792071884447,"version":"4.4.0"},"reference-count":76,"publisher":"American Chemical Society (ACS)","issue":"41","license":[{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"funder":[{"DOI":"10.13039\/100000928","name":"Welch Foundation","doi-asserted-by":"publisher","award":["F-1891"],"award-info":[{"award-number":["F-1891"]}],"id":[{"id":"10.13039\/100000928","id-type":"DOI","asserted-by":"publisher"}]},{"name":"National Institutes of Health (NIH)","award":["R35GM133359"],"award-info":[{"award-number":["R35GM133359"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,10,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Lipid nanoparticles (LNPs) are the leading platform for RNA delivery, enabling applications ranging from mRNA vaccines to CRISPR therapeutics. Ionizable lipids, such as DLin-MC3-DMA (MC3), play a central role in nucleic acid encapsulation and intracellular delivery, yet the molecular mechanisms governing LNP structure and function remain incompletely understood. The internal organization of LNP cores is highly sensitive to pH and has been described by inverse hexagonal, cubic, and lamellar phases that influence delivery performance. Here, we investigate the pH-dependent structure and dynamics of MC3-containing LNPs using two-dimensional infrared (2D IR) spectroscopy and molecular dynamics (MD) simulations. Both experiments and simulations reveal pronounced pH-dependent changes in the local environment surrounding the lipid ester linkage. At pH 4, strong electrostatic interactions between RNA and protonated MC3 produce broader spectral features consistent with increased interfacial heterogeneity and hydration. In addition, the frequency fluctuation dynamics exhibit a 46% slowdown in RNA-loaded LNPs relative to empty LNPs. In contrast, increasing the pH from 4 to 8 narrows the ester carbonyl band by \u223c10 cm\u20131 and yields nearly identical dynamics for loaded and empty LNPs, indicating a more homogeneous and less hydrated lipid interface. MD simulations further demonstrate that MC3 protonation modulates RNA binding, interfacial hydration, and lipid packing. Together, these results highlight RNA-lipid electrostatic interactions as an important factor influencing the local environment within LNPs and potentially their pH-dependent behavior, providing molecular-level insights into the rational design of next-generation RNA delivery systems.<\/jats:p>","DOI":"10.1021\/acs.jpcb.6c04413","type":"journal-article","created":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T17:22:47Z","timestamp":1790875367000},"page":"10930-10940","source":"Crossref","is-referenced-by-count":0,"title":["RNA-Lipid Interactions\nControl Structure and Dynamics\nof Lipid Nanoparticles"],"prefix":"10.1021","volume":"130","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6107-1713","authenticated-orcid":true,"given":"Xiaobing","family":"Chen","sequence":"first","affiliation":[{"name":"Augusta University , , , ,","place":["Augusta, Georgia, United States, 30912"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7102-2851","authenticated-orcid":true,"given":"Cong","family":"Xu","sequence":"additional","affiliation":[{"name":"University of Texas at Austin , , , ,","place":["Austin, Texas, United States, 78712"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0699-8468","authenticated-orcid":true,"given":"Carlos R.","family":"Baiz","sequence":"additional","affiliation":[{"name":"University of Texas at Austin , , , ,","place":["Austin, Texas, United States, 78712"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,10,1]]},"reference":[{"issue":"5","key":"2026101508061141200_cit1","doi-asserted-by":"publisher","first-page":"4787","DOI":"10.1021\/acsnano.8b01516","article-title":"On the Formation\nand Morphology of Lipid Nanoparticles Containing Ionizable Cationic\nLipids and SiRNA","volume":"12","author":"Kulkarni","year":"2018","journal-title":"ACS Nano"},{"issue":"2","key":"2026101508061141200_cit2","doi-asserted-by":"publisher","first-page":"101463","DOI":"10.1016\/j.omtm.2025.101463","article-title":"Lipid Nanoparticles:\nComposition, Formulation, and Application","volume":"33","author":"Xu","year":"2025","journal-title":"Mol.\nTher., Methods Clin. Dev."},{"key":"2026101508061141200_cit3","doi-asserted-by":"publisher","first-page":"11","DOI":"10.1056\/nejmoa1716153","article-title":"an RNAi\nTherapeutic, for Hereditary Transthyretin Amyloidosis","volume":"379","author":"Adams","year":"2018","journal-title":"N. Engl. J. Med."},{"key":"2026101508061141200_cit4","doi-asserted-by":"publisher","first-page":"1078","DOI":"10.1038\/s41578-021-00358-0","article-title":"Lipid Nanoparticles\nfor MRNA Delivery","volume-title":"Nature Reviews Materials","author":"Hou","year":"2021"},{"issue":"11","key":"2026101508061141200_cit5","doi-asserted-by":"publisher","first-page":"16982","DOI":"10.1021\/acsnano.1c04996","article-title":"Lipid Nanoparticles\u2013From\nLiposomes to MRNA Vaccine Delivery, a Landscape of Research Diversity\nand Advancement","volume":"15","author":"Tenchov","year":"2021","journal-title":"ACS Nano"},{"key":"2026101508061141200_cit6","doi-asserted-by":"publisher","first-page":"101705","DOI":"10.1016\/j.cocis.2023.101705","article-title":"Review of\nStructural Design Guiding the Development of Lipid Nanoparticles for\nNucleic Acid Delivery","volume":"66","author":"Ca\u0301rdenas","year":"2023","journal-title":"Curr. Opin. Colloid Interface\nSci."},{"issue":"1","key":"2026101508061141200_cit7","doi-asserted-by":"publisher","first-page":"2","DOI":"10.1021\/acs.accounts.1c00544","article-title":"Chemistry of Lipid\nNanoparticles for RNA Delivery","volume":"55","author":"Eygeris","year":"2022","journal-title":"Acc. Chem. Res."},{"issue":"2","key":"2026101508061141200_cit8","doi-asserted-by":"publisher","first-page":"172","DOI":"10.1038\/nbt.1602","article-title":"Rational\nDesign of Cationic Lipids for SiRNA Delivery","volume":"28","author":"Semple","year":"2010","journal-title":"Nat. Biotechnol."},{"issue":"34","key":"2026101508061141200_cit9","doi-asserted-by":"publisher","first-page":"8529","DOI":"10.1002\/anie.201203263","article-title":"Maximizing the Potency of SiRNA Lipid Nanoparticles\nfor Hepatic Gene Silencing in Vivo","volume":"51","author":"Jayaraman","year":"2012","journal-title":"Angew. Chem.,\nInt. Ed."},{"issue":"1","key":"2026101508061141200_cit13","doi-asserted-by":"publisher","first-page":"956","DOI":"10.1038\/s42003-021-02441-2","article-title":"Ionization and Structural Properties\nof MRNA Lipid Nanoparticles Influence Expression in Intramuscular\nand Intravascular Administration","volume":"4","author":"Carrasco","year":"2021","journal-title":"Commun. Biol."},{"key":"2026101508061141200_cit14","doi-asserted-by":"publisher","first-page":"709","DOI":"10.1038\/s41573-024-00977-6","article-title":"The 60-Year Evolution\nof Lipid Nanoparticles for Nucleic\nAcid Delivery","volume":"23","author":"Cullis","year":"2024","journal-title":"Nat. Rev. Drug Discovery"},{"key":"2026101508061141200_cit15","doi-asserted-by":"publisher","first-page":"417","DOI":"10.1038\/s44222-026-00401-1","article-title":"Design\nPrinciples of Lipid Nanoparticles for RNA Delivery","volume":"4","author":"Arral","year":"2026","journal-title":"Nature Reviews Bioengineering"},{"key":"2026101508061141200_cit16","doi-asserted-by":"publisher","first-page":"114416","DOI":"10.1016\/j.addr.2022.114416","article-title":"The Role of Lipid\nComponents in Lipid Nanoparticles\nfor Vaccines and Gene Therapy","volume":"188","author":"Albertsen","year":"2022","journal-title":"Adv. Drug Delivery\nRev."},{"key":"2026101508061141200_cit18","doi-asserted-by":"publisher","first-page":"11454","DOI":"10.1021\/acsnano.3c01186","article-title":"Correlating the\nStructure and Gene Silencing Activity of Oligonucleotide-Loaded Lipid\nNanoparticles Using Small-Angle X-Ray Scattering","volume":"17","author":"Hammel","year":"2023","journal-title":"ACS Nano"},{"key":"2026101508061141200_cit19","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2310491120","article-title":"Dependent Structural Transitions in Cationic Ionizable\nLipid Mesophases Are Critical for Lipid Nanoparticle Function","volume":"120","author":"Philipp","year":"2023","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"issue":"3","key":"2026101508061141200_cit20","doi-asserted-by":"publisher","first-page":"690","DOI":"10.1016\/j.xphs.2021.11.004","article-title":"PH-Dependent\nPhase Behavior and Stability of Cationic Lipid\u2013MRNA Nanoparticles","volume":"111","author":"Larson","year":"2022","journal-title":"J. Pharm. Sci."},{"issue":"2","key":"2026101508061141200_cit21","doi-asserted-by":"publisher","first-page":"276","DOI":"10.1016\/j.jconrel.2005.06.014","article-title":"Cationic Lipid\nSaturation Influences Intracellular Delivery of Encapsulated Nucleic\nAcids","volume":"107","author":"Heyes","year":"2005","journal-title":"J. Controlled Release"},{"issue":"20","key":"2026101508061141200_cit23","doi-asserted-by":"publisher","first-page":"3210","DOI":"10.1021\/acs.accounts.5c00522","article-title":"Ionizable Lipid Nanoparticles\nfor MRNA Delivery: Internal Self-Assembled Inverse Mesophase Structure\nand Endosomal Escape","volume":"58","author":"Yu","year":"2025","journal-title":"Acc. Chem. Res."},{"issue":"1","key":"2026101508061141200_cit24","doi-asserted-by":"publisher","first-page":"1303","DOI":"10.1038\/s41467-024-45666-5","article-title":"Liquid Crystalline\nInverted Lipid Phases Encapsulating\nSiRNA Enhance Lipid Nanoparticle Mediated Transfection","volume":"15","author":"Pattipeiluhu","year":"2024","journal-title":"Nat. Commun."},{"key":"2026101508061141200_cit28","doi-asserted-by":"publisher","first-page":"24765","DOI":"10.1021\/jacs.3c08729","article-title":"Inverse Cubic and Hexagonal\nMesophase Evolution within Ionizable\nLipid Nanoparticles Correlates with MRNA Transfection in Macrophages","volume":"145","author":"Yu","year":"2023","journal-title":"J. Am. Chem. Soc."},{"issue":"11","key":"2026101508061141200_cit29","doi-asserted-by":"publisher","first-page":"10829","DOI":"10.1021\/acsnano.4c14098","article-title":"Buffer\nSpecificity of Ionizable Lipid Nanoparticle\nTransfection Efficiency and Bulk Phase Transition","volume":"19","author":"Carucci","year":"2025","journal-title":"ACS Nano"},{"issue":"2","key":"2026101508061141200_cit30","doi-asserted-by":"publisher","DOI":"10.1073\/PNAS.2311700120","article-title":"Lipid Shape and Packing\nAre Key for Optimal Design of PH-Sensitive MRNA Lipid Nanoparticles","volume":"121","author":"Tesei","year":"2024","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"key":"2026101508061141200_cit31","doi-asserted-by":"publisher","first-page":"17972","DOI":"10.1021\/acsnano.5c22170","article-title":"Storage Buffer Composition\nImpacts Internal Structure, Freeze\u2013Thaw Stability, and Transfection\nEfficiency of MRNA-Lipid Nanoparticles","volume":"20","author":"Mohammadi-Zerankeshi","year":"2026","journal-title":"ACS\nNano"},{"issue":"15","key":"2026101508061141200_cit32","doi-asserted-by":"publisher","first-page":"E3351","DOI":"10.1073\/PNAS.1720542115","article-title":"Successful Reprogramming\nof Cellular Protein Production through MRNA Delivered by Functionalized\nLipid Nanoparticles","volume":"115","author":"Arteta","year":"2018","journal-title":"Proc. Natl. Acad. Sci.\nU. S. A."},{"issue":"5","key":"2026101508061141200_cit33","doi-asserted-by":"publisher","first-page":"1158","DOI":"10.1021\/acs.jpcb.2c07671","article-title":"Atomistic Insights into Organization\nof RNA-Loaded Lipid Nanoparticles","volume":"127","author":"Paloncy\u0301ova\u0301","year":"2023","journal-title":"J. Phys.\nChem. B"},{"key":"2026101508061141200_cit34","doi-asserted-by":"crossref","DOI":"10.1017\/CBO9780511675935","volume-title":"Concepts and Methods of\n2D Infrared Spectroscopy","author":"Hamm","year":"2011"},{"key":"2026101508061141200_cit35","doi-asserted-by":"publisher","first-page":"704","DOI":"10.1002\/lapl.200710046","article-title":"Ultrafast\n2D-IR Vibrational Echo\nSpectroscopy: A Probe of Molecular Dynamics","volume":"4","author":"Park","year":"2007","journal-title":"Laser Phys. Lett."},{"key":"2026101508061141200_cit36","doi-asserted-by":"publisher","first-page":"10726","DOI":"10.1021\/acs.chemrev.6b00582","article-title":"Watching Proteins Wiggle: Mapping\nStructures with Two-Dimensional Infrared Spectroscopy","volume":"117","author":"Ghosh","year":"2017","journal-title":"Chem. Rev."},{"key":"2026101508061141200_cit37","doi-asserted-by":"publisher","first-page":"4093","DOI":"10.1021\/acs.jpclett.0c00783","article-title":"Ultrafast\nSpectroscopy of Lipid-Water\nInterfaces: Transmembrane Crowding Drives H-Bond Dynamics","volume":"11","author":"Flanagan","year":"2020","journal-title":"J. Phys. Chem. Lett."},{"key":"2026101508061141200_cit38","doi-asserted-by":"publisher","first-page":"15650","DOI":"10.1021\/ja205636h","article-title":"Anharmonic Vibrational Modes of Nucleic\nAcid Bases Revealed by 2D IR Spectroscopy","volume":"133","author":"Peng","year":"2011","journal-title":"J.\nAm. Chem. Soc."},{"key":"2026101508061141200_cit39","doi-asserted-by":"publisher","first-page":"685","DOI":"10.1021\/acs.accounts.3c00682","article-title":"Using 2D-IR\nSpectroscopy to Measure the Structure, Dynamics, and Intermolecular\nInteractions of Proteins in H2O","volume":"57","author":"Hunt","year":"2024","journal-title":"Acc. Chem.\nRes."},{"key":"2026101508061141200_cit40","doi-asserted-by":"publisher","first-page":"9602","DOI":"10.1021\/acs.jpclett.1c02451","article-title":"Molecular Mechanism of Cell Membrane\nProtection by Sugars: A Study of Interfacial H-Bond Networks","volume":"12","author":"You","year":"2021","journal-title":"J. Phys. Chem. Lett."},{"key":"2026101508061141200_cit41","doi-asserted-by":"publisher","first-page":"900","DOI":"10.1038\/s42003-023-05274-3","article-title":"Crowding\nAlters F-Actin Secondary Structure and Hydration","volume":"6","author":"Chen","year":"2023","journal-title":"Commun. Biol."},{"key":"2026101508061141200_cit42","doi-asserted-by":"publisher","first-page":"1801","DOI":"10.1021\/jacsau.5c01692","article-title":"Solvent\nReorganization in Stabilized Protein\u2013Polymer Conjugates Visualized\nby Two-Dimensional Infrared and Nuclear Magnetic Resonance Spectroscopy","volume":"6","author":"Maia","year":"2026","journal-title":"JACS Au"},{"issue":"14","key":"2026101508061141200_cit43","doi-asserted-by":"publisher","first-page":"E3126","DOI":"10.1073\/pnas.1722042115","article-title":"Coordination to\nLanthanide Ions Distorts Binding Site\nConformation in Calmodulin","volume":"115","author":"Edington","year":"2018","journal-title":"Proc. Natl. Acad.\nSci. U. S. A."},{"issue":"11","key":"2026101508061141200_cit44","doi-asserted-by":"publisher","first-page":"1859","DOI":"10.1002\/jcc.20945","article-title":"CHARMM-GUI: A Web-Based Graphical\nUser Interface for CHARMM","volume":"29","author":"Jo","year":"2008","journal-title":"J. Comput. Chem."},{"issue":"8016","key":"2026101508061141200_cit45","doi-asserted-by":"publisher","first-page":"493","DOI":"10.1038\/s41586-024-07487-w","article-title":"Accurate Structure\nPrediction of\nBiomolecular Interactions with AlphaFold 3","volume":"630","author":"Abramson","year":"2024","journal-title":"Nature"},{"key":"2026101508061141200_cit46","unstructured":"Bauer, P.; Hess, B.; Lindahl, E.\n          GROMACS 2022.1 Source\nCode; Zenodo, 2022.10.5281\/zenodo.6451564."},{"issue":"1","key":"2026101508061141200_cit47","doi-asserted-by":"publisher","first-page":"71","DOI":"10.1038\/nmeth.4067","article-title":"CHARMM36m:\nAn Improved Force Field for Folded and Intrinsically Disordered Proteins","volume":"14","author":"Huang","year":"2017","journal-title":"Nat. Methods"},{"issue":"2","key":"2026101508061141200_cit48","doi-asserted-by":"publisher","first-page":"926","DOI":"10.1063\/1.445869","article-title":"Comparison\nof Simple Potential Functions for Simulating Liquid Water","volume":"79","author":"Jorgensen","year":"1983","journal-title":"J. Chem. Phys."},{"issue":"14","key":"2026101508061141200_cit49","doi-asserted-by":"publisher","first-page":"2001","DOI":"10.1016\/j.bpj.2023.12.019","article-title":"Binding-Induced\nLipid Domains: Peptide-Membrane Interactions with PIP2 and PS","volume":"123","author":"Al-Mualem","year":"2024","journal-title":"Biophys. J."},{"issue":"7","key":"2026101508061141200_cit50","doi-asserted-by":"publisher","first-page":"1146","DOI":"10.1016\/j.bpj.2025.02.019","article-title":"Cooperativity of PIP2 and PS Lipids\nModulates PH Domain\nBinding","volume":"124","author":"Chen","year":"2025","journal-title":"Biophys. J."},{"issue":"8","key":"2026101508061141200_cit51","doi-asserted-by":"publisher","first-page":"1541","DOI":"10.1016\/j.bpj.2018.08.044","article-title":"Physiological Calcium\nConcentrations Slow Dynamics at the Lipid-Water Interface","volume":"115","author":"Valentine","year":"2018","journal-title":"Biophys. J."},{"key":"2026101508061141200_cit52","doi-asserted-by":"publisher","first-page":"283","DOI":"10.1146\/annurev-physchem-090722-010230","article-title":"Lipid Landscapes:\nVibrational Spectroscopy\nfor Decoding Membrane Complexity","volume":"75","author":"Chen","year":"2024","journal-title":"Annu. Rev.\nPhys. Chem."},{"issue":"29","key":"2026101508061141200_cit53","doi-asserted-by":"publisher","first-page":"6498","DOI":"10.1021\/acs.jpca.1c04558","article-title":"Pump Slice Amplitudes: A Simple and\nRobust Method for Connecting Two-Dimensional Infrared and Fourier\nTransform Infrared Spectra","volume":"125","author":"Valentine","year":"2021","journal-title":"J. Phys. Chem. A"},{"issue":"22","key":"2026101508061141200_cit54","doi-asserted-by":"publisher","first-page":"3888","DOI":"10.1021\/acs.jpca.6b02887","article-title":"An Empirical\nIR Frequency Map for\nEster C = O Stretching Vibrations","volume":"120","author":"Edington","year":"2016","journal-title":"J. Phys.\nChem. A"},{"issue":"20","key":"2026101508061141200_cit57","doi-asserted-by":"publisher","first-page":"5160","DOI":"10.1021\/acs.jpclett.7b02057","article-title":"Cooperative Hydrogen-Bond Dynamics at a Zwitterionic\nLipid\/Water Interface Revealed by 2D HD-VSFG Spectroscopy","volume":"8","author":"Inoue","year":"2017","journal-title":"J. Phys. Chem. Lett."},{"issue":"7","key":"2026101508061141200_cit58","doi-asserted-by":"publisher","first-page":"075102","DOI":"10.1063\/5.0080153","article-title":"Interfacial Dynamics in Inverted-Headgroup\nLipid Membranes","volume":"156","author":"Lee","year":"2022","journal-title":"J. Chem. Phys."},{"issue":"9","key":"2026101508061141200_cit59","doi-asserted-by":"publisher","first-page":"1860","DOI":"10.1021\/acs.accounts.0c00302","article-title":"Ultrafast Dynamics at Lipid-Water\nInterfaces","volume":"53","author":"Flanagan","year":"2020","journal-title":"Acc. Chem. Res."},{"key":"2026101508061141200_cit61","doi-asserted-by":"publisher","first-page":"113879","DOI":"10.1016\/j.jconrel.2025.113879","article-title":"A Perspective on the\nApparent PKa of Ionizable Lipids\nin MRNA-LNPs","volume":"384","author":"Simonsen","year":"2025","journal-title":"J. Controlled Release"},{"issue":"38","key":"2026101508061141200_cit62","doi-asserted-by":"publisher","first-page":"9151","DOI":"10.1021\/acs.jpcb.4c03747","article-title":"Exploring\nPH-Triggered Lamellar to Cubic Phase Transition in 2-Hydroxyoleic\nAcid\/Monoolein Nanodispersions: Insights into Membrane Physicochemical\nProperties","volume":"128","author":"Rui","year":"2024","journal-title":"J. Phys. Chem. B"},{"issue":"4","key":"2026101508061141200_cit63","doi-asserted-by":"publisher","first-page":"101596","DOI":"10.1016\/j.omtm.2025.101596","article-title":"Structuring of Lipid\nNanoparticle MRNA Formulations at Acidic and Neutral PH: X-Ray Scattering\nand Molecular Dynamics Studies","volume":"33","author":"Guruge","year":"2025","journal-title":"Mol. Ther.,\nMethods Clin. Dev."},{"issue":"41","key":"2026101508061141200_cit64","doi-asserted-by":"publisher","first-page":"8049","DOI":"10.1039\/D5SM00666J","article-title":"Combining\nSAXS Analysis and MD Simulation to Determine\nStructure and Hydration of Ionizable Lipid Hexagonal Phases","volume":"21","author":"Philipp","year":"2025","journal-title":"Soft Matter"},{"issue":"2","key":"2026101508061141200_cit65","doi-asserted-by":"publisher","first-page":"979","DOI":"10.1021\/acsnano.2c06213","article-title":"Acidification-Induced\nStructure Evolution of Lipid Nanoparticles Correlates with Their In\nVitro Gene Transfections","volume":"17","author":"Li","year":"2023","journal-title":"ACS Nano"},{"issue":"42","key":"2026101508061141200_cit66","doi-asserted-by":"publisher","first-page":"16534","DOI":"10.1073\/pnas.0707431104","article-title":"Self-Assembled\nLamellar Complexes of SiRNA with Lipidic Aminoglycoside Derivatives\nPromote Efficient SiRNA Delivery and Interference","volume":"104","author":"Desigaux","year":"2007","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"issue":"4","key":"2026101508061141200_cit67","doi-asserted-by":"publisher","first-page":"2193","DOI":"10.1021\/acs.molpharmaceut.4c01437","article-title":"Molecular\nInsight into Lipid Nanoparticle Assembly from NMR Spectroscopy and\nMolecular Dynamics Simulation","volume":"22","author":"Li","year":"2025","journal-title":"Mol. Pharmaceutics"},{"key":"2026101508061141200_cit68","doi-asserted-by":"publisher","first-page":"120586","DOI":"10.1016\/j.ijpharm.2021.120586","article-title":"MRNA-Lipid Nanoparticle COVID-19\nVaccines: Structure and Stability","volume":"601","author":"Schoenmaker","year":"2021","journal-title":"Int. J. Pharm."},{"issue":"20","key":"2026101508061141200_cit69","doi-asserted-by":"publisher","first-page":"3927","DOI":"10.1016\/j.bpj.2022.08.037","article-title":"MRNA Lipid Nanoparticle Phase Transition","volume":"121","author":"Trollmann","year":"2022","journal-title":"Biophys. J."},{"issue":"18","key":"2026101508061141200_cit70","doi-asserted-by":"publisher","first-page":"7842","DOI":"10.1021\/ja300658h","article-title":"Three Distinct Water\nStructures at a Zwitterionic Lipid\/Water Interface Revealed by Heterodyne-Detected\nVibrational Sum Frequency Generation","volume":"134","author":"Mondal","year":"2012","journal-title":"J. Am.\nChem. Soc."},{"issue":"0","key":"2026101508061141200_cit71","doi-asserted-by":"publisher","first-page":"317","DOI":"10.1039\/D3FD00117B","article-title":"Biological\nLipid Hydration: Distinct Mechanisms of Interfacial Water Alignment\nand Charge Screening for Model Lipid Membranes","volume":"249","author":"Saak","year":"2024","journal-title":"Faraday Discuss."},{"issue":"48","key":"2026101508061141200_cit72","doi-asserted-by":"publisher","first-page":"25274","DOI":"10.1002\/anie.202110975","article-title":"Probing Water State during Lipidic Mesophases Phase\nTransitions","volume":"60","author":"Yao","year":"2021","journal-title":"Angew. Chem., Int. Ed."},{"issue":"1","key":"2026101508061141200_cit73","doi-asserted-by":"publisher","first-page":"126","DOI":"10.1039\/C1AN15628D","article-title":"Resonant Mie\nScattering (RMieS) Correction\nApplied to FTIR Images of Biological Tissue Samples","volume":"137","author":"Bambery","year":"2012","journal-title":"Analyst"},{"issue":"50","key":"2026101508061141200_cit74","doi-asserted-by":"publisher","first-page":"61710","DOI":"10.1021\/acsomega.5c07884","article-title":"A Universal Approach to Mie Scatter\nCorrection in FTIR Analysis of Microsized Samples","volume":"10","author":"Blazhko","year":"2025","journal-title":"ACS Omega"},{"issue":"8","key":"2026101508061141200_cit75","doi-asserted-by":"publisher","first-page":"1586","DOI":"10.1039\/b904808a","article-title":"Resonant Mie Scattering in Infrared\nSpectroscopy of\nBiological Materials \u2013 Understanding the \u2018Dispersion\nArtefact","volume":"134","author":"Bassan","year":"2009","journal-title":"Analyst"},{"key":"2026101508061141200_cit76","volume-title":"Light\nScattering by Small Particles","author":"van de Hulst","year":"1981"},{"issue":"11","key":"2026101508061141200_cit77","first-page":"133","article-title":"Lorenz-Mie\nLight Scattering in Cellular Biology","volume":"2","author":"Ulicny\u0301","year":"1992","journal-title":"Gen. Physiol.\nBiophys."},{"issue":"35","key":"2026101508061141200_cit78","doi-asserted-by":"publisher","first-page":"5881","DOI":"10.1021\/acs.jpca.2c03803","article-title":"Importance\nof Hydrogen Bonding in Crowded Environments:\nA Physical Chemistry Perspective","volume":"126","author":"You","year":"2022","journal-title":"J. Phys. Chem.\nA"},{"issue":"25","key":"2026101508061141200_cit79","doi-asserted-by":"publisher","first-page":"6200","DOI":"10.1021\/acs.jpcb.5c01399","article-title":"Molecular\nDynamics\nof Lipid Nanoparticles Explored through Solution and Solid-State NMR\nSpectroscopy and MD Simulations","volume":"129","author":"Schroder","year":"2025","journal-title":"J. Phys. Chem.\nB"},{"issue":"1","key":"2026101508061141200_cit82","doi-asserted-by":"publisher","first-page":"36645","DOI":"10.1038\/s41598-025-20340-y","article-title":"Driving Forces in the\nAssembly of Lipid Nanoparticles\nContaining MRNA Revealed by Molecular Dynamics Simulations at Acidic\nand Physiological PH","volume":"15","author":"Hardianto","year":"2025","journal-title":"Sci. Rep."},{"issue":"25","key":"2026101508061141200_cit83","doi-asserted-by":"publisher","first-page":"16806","DOI":"10.1039\/C7CP01921A","article-title":"Determination\nof Bending Rigidity\nand Tilt Modulus of Lipid Membranes from Real-Space Fluctuation Analysis\nof Molecular Dynamics Simulations","volume":"19","author":"Doktorova","year":"2017","journal-title":"Phys. Chem.\nChem. Phys."},{"issue":"1","key":"2026101508061141200_cit84","doi-asserted-by":"publisher","first-page":"16078","DOI":"10.1038\/s41598-017-16450-x","article-title":"The Influence of Curvature on the\nProperties of the Plasma Membrane. Insights from Atomistic Molecular\nDynamics Simulations","volume":"7","author":"Yesylevskyy","year":"2017","journal-title":"Sci. Rep."},{"issue":"29","key":"2026101508061141200_cit85","doi-asserted-by":"publisher","first-page":"6299","DOI":"10.1021\/acs.jpcb.0c04253","article-title":"On Calculating\nthe Bending Modulus of Lipid Bilayer Membranes from Buckling Simulations","volume":"124","author":"Eid","year":"2020","journal-title":"J. Phys. Chem. B"},{"key":"2026101508061141200_cit86","doi-asserted-by":"publisher","first-page":"23365","DOI":"10.1073\/pnas.2008789117","article-title":"Scaling\nRelationships for the Elastic\nModuli and Viscosity of Mixed Lipid Membranes","volume":"117","author":"Kelley","year":"2020","journal-title":"Proc. Natl. Acad. Sci. U. S. A."},{"issue":"16","key":"2026101508061141200_cit88","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.202505261","article-title":"From RNA to DNA: How Cargo Identity\nReprograms Lipid Nanoparticle Architecture and Function","volume":"15","author":"Quagliarini","year":"2026","journal-title":"Adv. Healthcare Mater."},{"key":"2026101508061141200_cit89","doi-asserted-by":"publisher","first-page":"265","DOI":"10.1038\/s41596-022-00755-x","article-title":"Preparation of Selective Organ-Targeting\n(SORT) Lipid Nanoparticles (LNPs) Using Multiple Technical Methods\nfor Tissue-Specific MRNA Delivery","volume":"18","author":"Wang","year":"2023","journal-title":"Nat. Protoc."},{"key":"2026101508061141200_cit90","doi-asserted-by":"publisher","first-page":"313","DOI":"10.1038\/s41565-020-0669-6","article-title":"Selective\nOrgan Targeting (SORT) Nanoparticles for Tissue-Specific MRNA Delivery\nand CRISPR\u2013Cas Gene Editing","volume":"15","author":"Cheng","year":"2020","journal-title":"Nat. Nanotechnol."}],"container-title":["The Journal of Physical\nChemistry B"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acs.jpcb.6c04413","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/jpcbfk\/article-pdf\/130\/41\/10930\/68729130\/acs.jpcb.6c04413.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/jpcbfk\/article-pdf\/130\/41\/10930\/68729130\/acs.jpcb.6c04413.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T12:49:30Z","timestamp":1792068570000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/jpcbfk\/article\/130\/41\/10930\/5443509\/RNA-Lipid-Interactions-Control-Structure-and"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10,1]]},"references-count":76,"journal-issue":{"issue":"41","published-online":{"date-parts":[[2026,10,1]]},"published-print":{"date-parts":[[2026,10,15]]}},"URL":"https:\/\/doi.org\/10.1021\/acs.jpcb.6c04413","relation":{},"ISSN":["1520-6106","1520-5207"],"issn-type":[{"value":"1520-6106","type":"print"},{"value":"1520-5207","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10,1]]}}}