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While they are usually visualized by staining with Thioflavin-T, Congo Red, or other fluorescent markers, it still arouses a controversy over possible staining molecule influence on the amyloid structure or aggregation process. In this work we present, for the first time, the polarization analysis of two-photon excited autofluorescence of amyloids and confirm that polarization dependence of the observed emission can be correlated with the orientation of fibrils. We show the potential of two-photon excited autofluorescence for resolution of molecular organization of fibrils within amyloid superstructures. This label-free method is compatible with two-photon imaging already applied in investigation of neurodegeneration model in mice.<\/jats:p>","DOI":"10.1021\/acs.jpclett.0c03511","type":"journal-article","created":{"date-parts":[[2021,2,2]],"date-time":"2021-02-02T03:38:27Z","timestamp":1612237107000},"page":"1432-1437","source":"Crossref","is-referenced-by-count":11,"title":["Two-Photon Excited Polarization-Dependent Autofluorescence\nof Amyloids as a Label-Free Method of Fibril Organization Imaging"],"prefix":"10.1021","volume":"12","author":[{"given":"Patryk","family":"Obstarczyk","sequence":"first","affiliation":[{"name":"Wroclaw University of Science and Technology , , Wybrzez\u0307e Wyspian\u0301skiego 27 , ,","place":["Wroclaw, Poland, 50-370"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maciej","family":"Lipok","sequence":"additional","affiliation":[{"name":"Wroclaw University of Science and Technology , , Wybrzez\u0307e Wyspian\u0301skiego 27 , ,","place":["Wroclaw, Poland, 50-370"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8591-9387","authenticated-orcid":true,"given":"Manuela","family":"Grelich-Mucha","sequence":"additional","affiliation":[{"name":"Wroclaw University of Science and Technology , , Wybrzez\u0307e Wyspian\u0301skiego 27 , ,","place":["Wroclaw, Poland, 50-370"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5404-2455","authenticated-orcid":true,"given":"Marek","family":"Samoc\u0301","sequence":"additional","affiliation":[{"name":"Wroclaw University of Science and Technology , , Wybrzez\u0307e Wyspian\u0301skiego 27 , ,","place":["Wroclaw, Poland, 50-370"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7226-0077","authenticated-orcid":true,"given":"Joanna","family":"Olesiak-Ban\u0301ska","sequence":"additional","affiliation":[{"name":"Wroclaw University of Science and Technology , , Wybrzez\u0307e Wyspian\u0301skiego 27 , ,","place":["Wroclaw, Poland, 50-370"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2021,2,1]]},"reference":[{"issue":"12","key":"2026090307311311600_cit1","doi-asserted-by":"publisher","first-page":"755","DOI":"10.1038\/s41580-018-0060-8","article-title":"A new era\nfor understanding amyloid structures and disease","volume":"19","author":"Iadanza","year":"2018","journal-title":"Nat. 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