{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T14:51:45Z","timestamp":1786632705379,"version":"build-2736575974"},"reference-count":31,"publisher":"American Chemical Society (ACS)","issue":"32","license":[{"start":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T00:00:00Z","timestamp":1785283200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T00:00:00Z","timestamp":1785283200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T00:00:00Z","timestamp":1785283200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"funder":[{"DOI":"10.13039\/501100012226","name":"Fundamental Research Funds for the Central Universities","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100012226","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["12122402"],"award-info":[{"award-number":["12122402"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["12135003"],"award-info":[{"award-number":["12135003"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["12574221"],"award-info":[{"award-number":["12574221"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,8,13]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>How living cells transduce thermal perturbations into altered intracellular organization remains a fundamental biophysical question. Here, using single-particle tracking of quantum dot (QD) diffusion and endocytic vesicle transport, we show that temperature-enhanced transport is not a simple passive solvent effect; instead, heating enhances active cytoplasmic dynamics mediated by myosin-II-dependent actomyosin activity. While active fluctuations enhance random QD diffusion, they also accelerate active vesicular transport by reducing its mechanical resistance. Using thermoresponsive elastin-like polypeptide (ELPV) phase separation as a physical-chemical reporter, we demonstrate that myosin-II-dependent active fluctuations modulate phase separation. Near the transition temperature, the enhanced droplet formation after myosin-II inhibition suggests that active fluctuations oppose ELPV nucleation, whereas at higher temperatures, deeper within the two-phase regime, thermodynamic driving forces dominate. This work directly links nonequilibrium cytoplasmic mechanics with the physical chemistry of intracellular phase boundaries.<\/jats:p>","DOI":"10.1021\/acs.jpclett.6c02109","type":"journal-article","created":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T13:15:56Z","timestamp":1785849356000},"page":"9341-9347","source":"Crossref","is-referenced-by-count":0,"title":["Actomyosin Fluctuations\nCouple Temperature to Intracellular\nTransport and ELPV Phase Separation"],"prefix":"10.1021","volume":"17","author":[{"given":"Ming-Li","family":"Zhang","sequence":"first","affiliation":[{"name":"Beijing Normal University , , ,","place":["Beijing, China, 100875"]},{"name":"School of Systems Science and Institute of Nonequilibrium Systems, Beijing Normal University , ,","place":["Beijing, China, 100875"]},{"name":"Beijing Normal University , , ,","place":["Zhuhai, China, 519087"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zeyu","family":"Shen","sequence":"additional","affiliation":[{"name":"Southern University of Science and Technology , , ,","place":["Shenzhen, China, 518055"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zijin","family":"Li","sequence":"additional","affiliation":[{"name":"School of Systems Science and Institute of Nonequilibrium Systems, Beijing Normal University , ,","place":["Beijing, China, 100875"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junke","family":"Cheng","sequence":"additional","affiliation":[{"name":"School of Systems Science and Institute of Nonequilibrium Systems, Beijing Normal University , ,","place":["Beijing, China, 100875"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Xu","sequence":"additional","affiliation":[{"name":"Southern University of Science and Technology , , ,","place":["Shenzhen, China, 518055"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingcheng","family":"Yang","sequence":"additional","affiliation":[{"name":"Chinese Academy of Sciences , , ,","place":["Beijing, China, 100190"]},{"name":"University of Chinese Academy of Sciences , , ,","place":["Beijing, China, 100049"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaosong","family":"Chen","sequence":"additional","affiliation":[{"name":"Zhejiang University , , ,","place":["Hangzhou, China, 310058"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haiyun","family":"Ren","sequence":"additional","affiliation":[{"name":"Beijing Normal University , , ,","place":["Beijing, China, 100875"]},{"name":"Beijing Normal University , , ,","place":["Zhuhai, China, 519087"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0551-9912","authenticated-orcid":true,"given":"Hui","family":"Li","sequence":"additional","affiliation":[{"name":"Beijing Normal University , , ,","place":["Beijing, China, 100875"]},{"name":"School of Systems Science and Institute of Nonequilibrium Systems, Beijing Normal University , ,","place":["Beijing, China, 100875"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,7,29]]},"reference":[{"issue":"8","key":"2026081310070234900_cit1","doi-asserted-by":"publisher","first-page":"549","DOI":"10.1002\/andp.19053220806","article-title":"U\u0308ber\ndie von der molekularkinetischen Theorie der Wa\u0308rme geforderte\nBewegung von in ruhenden Flu\u0308ssigkeiten suspendierten Teilchen","volume":"322","author":"Einstein","year":"1905","journal-title":"Ann. 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