{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T19:44:48Z","timestamp":1792784688436,"version":"4.5.0"},"reference-count":33,"publisher":"American Chemical Society (ACS)","issue":"42","license":[{"start":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T00:00:00Z","timestamp":1791417600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T00:00:00Z","timestamp":1791417600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T00:00:00Z","timestamp":1791417600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"funder":[{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"publisher","award":["ANR-22-EXLU-0002"],"award-info":[{"award-number":["ANR-22-EXLU-0002"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"publisher","award":["ANR-23-CE29-0010"],"award-info":[{"award-number":["ANR-23-CE29-0010"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"publisher","award":["PEPRLUMA ULTRAFAST France 2030 (ANR-22-EXLU-0002)"],"award-info":[{"award-number":["PEPRLUMA ULTRAFAST France 2030 (ANR-22-EXLU-0002)"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]},{"name":"??cole Polytechnique","id":[{"id":"https:\/\/ror.org\/05hy3tk52","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,10,22]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>A promising fast, negative, red-absorbing photochromic system based on an intraprotein intermolecular charge transfer (CT) complex between a flavin cofactor and a substrate-analogue inhibitor MXA (methylthioacetate or methylselenoacetate; X = S, Se) within monomeric sarcosine oxidase (MSOX) displays MXA-independent, barrierless, high-yield femtosecond photoactivation associated with CT-interaction disruption coupled to MXA isomerization. Assessing the origin of the known MXA-dependence of the nanosecond thermal recovery requires a very precise determination of activation energy barriers. Here, a newly developed visible pump\u2013probe setup employing two Ti:sapphire femtosecond lasers spanning the picosecond to microsecond time scale was used. Whereas the room temperature thermal recovery time for MSeA is more than double that for MTA (7.5 ns vs 2.9 ns), the dissociation rate, the quantum yield (0.85), and recombination activation enthalpy (21 kJ\u00b7mol\u20131) are MXA-independent. Quantum mechanical calculations rationalize this value as the intrinsic enthalpic barrier for MXA isomerization. The observed variation in thermal recovery is due to the activation entropy, which is negative (on the order of \u221215 J\u00b7mol\u20131\u00b7K\u20131) and MXA-dependent. This dependence is attributed to a differently ordered transition state. Implications for the design and application of this class of photochromic systems are discussed.<\/jats:p>","DOI":"10.1021\/acs.jpclett.6c02645","type":"journal-article","created":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T13:02:04Z","timestamp":1791464524000},"page":"12125-12131","source":"Crossref","is-referenced-by-count":0,"title":["Characterization\nof Thermal Recovery Activation in\nFast Photochromic Flavoprotein Charge-Transfer Complexes"],"prefix":"10.1021","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7833-6204","authenticated-orcid":true,"given":"Amira Mounya","family":"Gharbi","sequence":"first","affiliation":[{"name":"Institut Polytechnique de Paris , , ,","place":["Palaiseau, France, 91120"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5995-5165","authenticated-orcid":true,"given":"Laura","family":"Antonucci","sequence":"additional","affiliation":[{"name":"Institut Polytechnique de Paris , , ,","place":["Palaiseau, France, 91120"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adeline","family":"Bonvalet","sequence":"additional","affiliation":[{"name":"Institut Polytechnique de Paris , , ,","place":["Palaiseau, France, 91120"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2459-4312","authenticated-orcid":true,"given":"Xavier","family":"Solinas","sequence":"additional","affiliation":[{"name":"Institut Polytechnique de Paris , , ,","place":["Palaiseau, France, 91120"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8264-0391","authenticated-orcid":true,"given":"Jean-Christophe","family":"Lambry","sequence":"additional","affiliation":[{"name":"Institut Polytechnique de Paris , , ,","place":["Palaiseau, France, 91120"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manuel","family":"Joffre","sequence":"additional","affiliation":[{"name":"Institut Polytechnique de Paris , , ,","place":["Palaiseau, France, 91120"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9464-1463","authenticated-orcid":true,"given":"Antonio","family":"Monari","sequence":"additional","affiliation":[{"name":"Universite\u0301 Paris Cite\u0301 and CNRS, ITODYS , ,","place":["Paris, France, F-75006"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9906-1190","authenticated-orcid":true,"given":"Bo","family":"Zhuang","sequence":"additional","affiliation":[{"name":"Peking University , , ,","place":["Beijing, China, 100871"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8150-3931","authenticated-orcid":true,"given":"Alexey","family":"Aleksandrov","sequence":"additional","affiliation":[{"name":"Institut Polytechnique de Paris , , ,","place":["Palaiseau, France, 91120"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0493-4831","authenticated-orcid":true,"given":"Marten H.","family":"Vos","sequence":"additional","affiliation":[{"name":"Institut Polytechnique de Paris , , ,","place":["Palaiseau, France, 91120"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5073-8180","authenticated-orcid":true,"given":"Michel","family":"Sliwa","sequence":"additional","affiliation":[{"name":"Institut Polytechnique de Paris , , ,","place":["Palaiseau, France, 91120"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,10,8]]},"reference":[{"issue":"5","key":"2026102208022790200_cit1","doi-asserted-by":"publisher","first-page":"2448","DOI":"10.1039\/D5NR04534G","article-title":"Reversibly\nPhotoswitchable Fluorescent Proteins: Integrating Photophysics, Photochemistry,\nBioimaging, and Protein Engineering","volume":"18","author":"Ozaki-Noma","year":"2026","journal-title":"Nanoscale"},{"key":"2026102208022790200_cit2","doi-asserted-by":"publisher","first-page":"241","DOI":"10.1007\/978-3-030-34413-9_9","article-title":"Reversibly Switchable\nFluorescent Proteins for RESOLFT Nanoscopy","volume-title":"Nanoscale Photonic Imaging","author":"Jensen","year":"2020"},{"issue":"11","key":"2026102208022790200_cit3","doi-asserted-by":"publisher","first-page":"685","DOI":"10.1038\/nrm.2017.71","article-title":"Fluorescence\nNanoscopy in Cell Biology","volume":"18","author":"Sahl","year":"2017","journal-title":"Nat. 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Commun."},{"issue":"18","key":"2026102208022790200_cit13","doi-asserted-by":"publisher","first-page":"3666","DOI":"10.1021\/acs.jpclett.6b01690","article-title":"Fast Photochromic\nMolecules toward\nRealization of Photosynergetic Effects","volume":"7","author":"Kobayashi","year":"2016","journal-title":"J. Phys.\nChem. Lett."},{"issue":"3","key":"2026102208022790200_cit14","doi-asserted-by":"publisher","first-page":"331","DOI":"10.1016\/S0969-2126(99)80043-4","article-title":"Monomeric\nSarcosine\nOxidase: Structure of a Covalently Flavinylated Amine Oxidizing Enzyme","volume":"7","author":"Trickey","year":"1999","journal-title":"Structure"},{"issue":"8","key":"2026102208022790200_cit15","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2118924119","article-title":"Ultrafast Photooxidation of Protein-Bound Anionic Flavin\nRadicals","volume":"119","author":"Zhuang","year":"2022","journal-title":"Proc. Natl. Acad. Sci. U. S. 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Flavin Reactivity and Active Site Binding Determinants","volume":"39","author":"Wagner","year":"2000","journal-title":"Biochemistry"},{"issue":"6","key":"2026102208022790200_cit18","doi-asserted-by":"publisher","DOI":"10.1002\/cptc.202500012","article-title":"Fast and Efficient\nRed-Absorbing Photoswitching Proteins Based on Flavin-Ligand Charge\nTransfer Complexes","volume":"9","author":"Zhuang","year":"2025","journal-title":"ChemPhotoChem."},{"issue":"26","key":"2026102208022790200_cit19","doi-asserted-by":"publisher","first-page":"11569","DOI":"10.1021\/jacs.2c04763","article-title":"Photoswitching Behavior\nof Flavin-Inhibitor Complex\nin a Nonphotocatalytic Flavoenzyme","volume":"144","author":"Zhuang","year":"2022","journal-title":"J. Am. Chem.\nSoc."},{"issue":"14","key":"2026102208022790200_cit20","doi-asserted-by":"publisher","first-page":"6079","DOI":"10.1039\/D4SC08614G","article-title":"Mechanism and Dynamics of Photoswitchable\nFlavoprotein Charge-Transfer Complexes","volume":"16","author":"Zhuang","year":"2025","journal-title":"Chem.\nSci."},{"issue":"16","key":"2026102208022790200_cit21","doi-asserted-by":"publisher","first-page":"17928","DOI":"10.1364\/OE.20.017928","article-title":"Asynchronous Optical\nSampling with Arbitrary Detuning between Laser Repetition Rates","volume":"20","author":"Antonucci","year":"2012","journal-title":"Opt. Express"},{"issue":"15","key":"2026102208022790200_cit22","doi-asserted-by":"publisher","first-page":"17811","DOI":"10.1364\/OE.25.017811","article-title":"Multiscale Control\nand Rapid Scanning of Time Delays Ranging from Picosecond to Millisecond","volume":"25","author":"Solinas","year":"2017","journal-title":"Opt. 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Biophys. Acta BBA - Bioenerg."},{"issue":"12","key":"2026102208022790200_cit28","doi-asserted-by":"publisher","first-page":"4227","DOI":"10.1021\/ja810032t","article-title":"A Fast Photochromic\nMolecule That Colors Only under UV Light","volume":"131","author":"Kishimoto","year":"2009","journal-title":"J.\nAm. Chem. Soc."},{"issue":"15","key":"2026102208022790200_cit29","doi-asserted-by":"publisher","first-page":"4952","DOI":"10.1021\/jacs.5b02353","article-title":"Photochromic Phenoxyl-Imidazolyl\nRadical Complexes with Decoloration Rates from Tens of Nanoseconds\nto Seconds","volume":"137","author":"Yamashita","year":"2015","journal-title":"J. Am. Chem. 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Chem."},{"issue":"5","key":"2026102208022790200_cit32","doi-asserted-by":"publisher","first-page":"3048","DOI":"10.1021\/jacsau.6c00440","article-title":"Ultrafast Photochemistry\nof Ligand-Bound Flavoprotein Amine Oxidases: Conformational Insights\nand Photocatalytic Implications","volume":"6","author":"Zhuang","year":"2026","journal-title":"JACS Au"},{"issue":"32","key":"2026102208022790200_cit33","doi-asserted-by":"publisher","first-page":"9955","DOI":"10.1002\/chem.201200497","article-title":"Supramolecular\nBinding Thermodynamics by Dispersion-Corrected Density Functional\nTheory","volume":"18","author":"Grimme","year":"2012","journal-title":"Chem. - Eur. J."}],"container-title":["The Journal of Physical\nChemistry Letters"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acs.jpclett.6c02645","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/jpclcd\/article-pdf\/doi\/10.1021\/acs.jpclett.6c02645\/68783262\/acs.jpclett.6c02645.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/jpclcd\/article-pdf\/doi\/10.1021\/acs.jpclett.6c02645\/68783262\/acs.jpclett.6c02645.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T12:29:31Z","timestamp":1792672171000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/jpclcd\/article\/17\/42\/12125\/5446818\/Characterization-of-Thermal-Recovery-Activation-in"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10,8]]},"references-count":33,"journal-issue":{"issue":"42","published-online":{"date-parts":[[2026,10,8]]},"published-print":{"date-parts":[[2026,10,22]]}},"URL":"https:\/\/doi.org\/10.1021\/acs.jpclett.6c02645","relation":{},"ISSN":["1948-7185"],"issn-type":[{"value":"1948-7185","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10,8]]}}}