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This methodology has been successfully used to validate structure hypotheses and to identify disorder. However, inherent distance limitations are under-explored. Many challenging biomolecular applications require long distance information, driving the need for a benchmarking study. We demonstrate distances up to 10.5 nm in TRIM25 reliably extracted from data obtained under standard sample conditions. By protein perdeuteration accurate distance measurements up to 17.3 nm were enabled. Enhancing sensitivity through optimization of the measurement and sample preparation conditions the achievable dipolar evolution time was extended to 150 \u03bcs. Thus, distances close to 20 nm should become experimentally accessible, highlighting the potential of advanced PDS methodologies for probing previously inaccessible long-range structural information in complex systems.<\/jats:p>","DOI":"10.1021\/acs.jpclett.6c02671","type":"journal-article","created":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T13:02:21Z","timestamp":1791205341000},"page":"11915-11920","source":"Crossref","is-referenced-by-count":0,"title":["Spins Afar\u2500Long-Range\nPulse Dipolar EPR Spectroscopy\nUncovers Curvature in TRIM25\u2019s Coiled Coil"],"prefix":"10.1021","volume":"17","author":[{"given":"Yannik","family":"Limbach","sequence":"first","affiliation":[{"name":"University of St.Andrews , , North Haugh, , 248 Scotland","place":["St Andrews, UK, KY16 9ST"]},{"name":"University of Bonn , , Wegelerstr. 12 , ,","place":["Bonn, Germany, 53115"]},{"name":"University of Dundee , , , ,","place":["Nethergate Dundee, Scotland, UK, DD1 4HN"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1632-0503","authenticated-orcid":true,"given":"Katrin","family":"Ackermann","sequence":"additional","affiliation":[{"name":"University of St.Andrews , , North Haugh, , 248 Scotland","place":["St Andrews, UK, KY16 9ST"]},{"name":"University of Dundee , , , ,","place":["Nethergate Dundee, Scotland, UK, DD1 4HN"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aisika","family":"Chakraborty","sequence":"additional","affiliation":[{"name":"University of St.Andrews , , North Haugh, , 248 Scotland","place":["St Andrews, UK, KY16 9ST"]},{"name":"University of Dundee , , , ,","place":["Nethergate Dundee, Scotland, UK, DD1 4HN"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7658-7720","authenticated-orcid":true,"given":"David G.","family":"Norman","sequence":"additional","affiliation":[{"name":"University of St.Andrews , , North Haugh, , 248 Scotland","place":["St Andrews, UK, KY16 9ST"]},{"name":"University of Dundee , , , ,","place":["Nethergate Dundee, Scotland, UK, DD1 4HN"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3384-271X","authenticated-orcid":true,"given":"Bela E.","family":"Bode","sequence":"additional","affiliation":[{"name":"University of St.Andrews , , North Haugh, , 248 Scotland","place":["St Andrews, UK, KY16 9ST"]},{"name":"University of Dundee , , , ,","place":["Nethergate Dundee, Scotland, UK, DD1 4HN"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,10,5]]},"reference":[{"issue":"1","key":"2026101508211187700_cit1","doi-asserted-by":"publisher","first-page":"46","DOI":"10.1016\/j.coi.2010.10.021","article-title":"Tripartite-motif\nproteins and innate immune regulation","volume":"23","author":"McNab","year":"2011","journal-title":"Curr.\nOpin. 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