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Osteoarthritis pathogenesis is yet to be fully elucidated with no osteoarthritis-specific biomarkers in clinical use. Ex vivo equine cartilage explants (n = 5) were incubated in tumor necrosis factor-\u03b1 (TNF-\u03b1)\/interleukin-1\u03b2 (IL-1\u03b2)-supplemented culture media for 8 days, with the media removed and replaced at 2, 5, and 8 days. Acetonitrile metabolite extractions of 8 day cartilage explants and media samples at all time points underwent one-dimensional (1D) 1H nuclear magnetic resonance metabolomic analysis, with media samples also undergoing mass spectrometry proteomic analysis. Within the cartilage, glucose and lysine were elevated following TNF-\u03b1\/IL-1\u03b2 treatment, while adenosine, alanine, betaine, creatine, myo-inositol, and uridine decreased. Within the culture media, 4, 4, and 6 differentially abundant metabolites and 154, 138, and 72 differentially abundant proteins were identified at 1\u20132, 3\u20135, and 6\u20138 days, respectively, including reduced alanine and increased isoleucine, enolase 1, vimentin, and lamin A\/C following treatment. Nine potential novel osteoarthritis neopeptides were elevated in the treated media. Implicated pathways were dominated by those involved in cellular movement. Our innovative study has provided insightful information on early osteoarthritis pathogenesis, enabling potential translation for clinical markers and possible new therapeutic targets.<\/jats:p>","DOI":"10.1021\/acs.jproteome.0c00143","type":"journal-article","created":{"date-parts":[[2020,7,23]],"date-time":"2020-07-23T08:58:59Z","timestamp":1595494739000},"page":"3652-3667","source":"Crossref","is-referenced-by-count":34,"title":["<i>Ex Vivo<\/i>\n                    Equine Cartilage Explant\nOsteoarthritis Model: A Metabolomics and Proteomics Study"],"prefix":"10.1021","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0489-7997","authenticated-orcid":true,"given":"James R.","family":"Anderson","sequence":"first","affiliation":[{"name":"University of Liverpool , , ,","place":["Liverpool, U.K., L7 8TX"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie M.","family":"Phelan","sequence":"additional","affiliation":[{"name":"University of Liverpool , , ,","place":["Liverpool, U.K., L69 7ZB"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Foddy","sequence":"additional","affiliation":[{"name":"University of Liverpool , , ,","place":["Liverpool, U.K., L69 3GH"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter D.","family":"Clegg","sequence":"additional","affiliation":[{"name":"University of Liverpool , , ,","place":["Liverpool, U.K., L7 8TX"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6979-0440","authenticated-orcid":true,"given":"Mandy J.","family":"Peffers","sequence":"additional","affiliation":[{"name":"University of Liverpool , , ,","place":["Liverpool, U.K., L7 8TX"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2020,7,23]]},"reference":[{"key":"2026090223354870900_cit1","doi-asserted-by":"publisher","first-page":"R188","DOI":"10.1186\/ar2101","article-title":"Differential\nGene Expression of Bone Anabolic Factors and Trabecular Bone Architectural\nChanges in the Proximal Femoral Shaft of Primary Hip Osteoarthritis\nPatients","volume":"8","author":"Truong","year":"2006","journal-title":"Arthritis Res. 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