{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T05:57:25Z","timestamp":1790834245490,"version":"4.1.0"},"reference-count":53,"publisher":"American Chemical Society (ACS)","issue":"6","license":[{"start":{"date-parts":[[2021,5,19]],"date-time":"2021-05-19T00:00:00Z","timestamp":1621382400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2021,5,19]],"date-time":"2021-05-19T00:00:00Z","timestamp":1621382400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2021,5,19]],"date-time":"2021-05-19T00:00:00Z","timestamp":1621382400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"funder":[{"DOI":"10.13039\/501100004826","name":"Natural Science Foundation of Beijing Municipality","doi-asserted-by":"publisher","award":["6182023"],"award-info":[{"award-number":["6182023"]}],"id":[{"id":"10.13039\/501100004826","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31872327"],"award-info":[{"award-number":["31872327"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010038","name":"Earmarked Fund for China Agriculture Research System","doi-asserted-by":"publisher","award":["CARS-36"],"award-info":[{"award-number":["CARS-36"]}],"id":[{"id":"10.13039\/501100010038","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Ningxia Elite High Producing Dairy Cattle Breeding Program","award":["2013NYYZ0501"],"award-info":[{"award-number":["2013NYYZ0501"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,6,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The aim of this study was to understand the molecular mechanisms behind the biological differences of X- and Y-sperm and to screen the sex-specific candidate antigen proteins for sexed semen production. To this end, we investigated differential expression of total membrane proteins of the two sperm types by using high-purity X- and Y-sperm from 20 Holstein bulls and applying the label-free proteomic technique; 1521 proteins were identified. In the X-sperm group, 8 and 23 proteins were significantly up- and down-regulated, respectively. In the X- and the Y-sperm group, 151 and 88 proteins were specifically expressed, respectively. These were overexpressed in the dynamic changes of the actin cytoskeleton, and cell senescence\/apoptosis induced by the immune response, and could result in differences in the state, size, and immune sensitivity of the X-\/Y-sperm membranes. The prediction of transmembrane structure, subcellular localization, and Western blotting validation results showed that the CLRN3 and SCAMP1 proteins were cell surface specific antigens of X- and Y-sperm, respectively. Our findings help explain the molecular mechanism behind the biological differences of X-\/Y-sperm and lay the foundation for application of immunological methods to produce sex-sorted semen and control livestock sex. Data are available via ProteomeXchange with identifier PXD019435.<\/jats:p>","DOI":"10.1021\/acs.jproteome.0c00358","type":"journal-article","created":{"date-parts":[[2021,5,19]],"date-time":"2021-05-19T15:21:50Z","timestamp":1621437710000},"page":"3031-3042","source":"Crossref","is-referenced-by-count":25,"title":["Differential Membrane\nProtein Profile in Bovine X-\nand Y\u2011Sperm"],"prefix":"10.1021","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2231-0224","authenticated-orcid":true,"given":"Dan","family":"Shen","sequence":"first","affiliation":[{"name":"China Agricultural University , , ,","place":["Beijing, China, 100193"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4539-1979","authenticated-orcid":true,"given":"Chenghao","family":"Zhou","sequence":"additional","affiliation":[{"name":"China Agricultural University , , ,","place":["Beijing, China, 100193"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingyue","family":"Cao","sequence":"additional","affiliation":[{"name":"China Agricultural University , , ,","place":["Beijing, China, 100193"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wentao","family":"Cai","sequence":"additional","affiliation":[{"name":"China Agricultural University , , ,","place":["Beijing, China, 100193"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongwei","family":"Yin","sequence":"additional","affiliation":[{"name":"China Agricultural University , , ,","place":["Beijing, China, 100193"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Jiang","sequence":"additional","affiliation":[{"name":"China Agricultural University , , ,","place":["Beijing, China, 100193"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shengli","family":"Zhang","sequence":"additional","affiliation":[{"name":"China Agricultural University , , ,","place":["Beijing, China, 100193"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2021,5,19]]},"reference":[{"key":"2026081415112348000_cit1","doi-asserted-by":"publisher","first-page":"s85","DOI":"10.1017\/S1751731118000496","article-title":"Review: Semen sexing - current state of the art with\nemphasis on bovine species","volume":"12","author":"Vishwanath","year":"2018","journal-title":"Animal"},{"issue":"Supplement\n5","key":"2026081415112348000_cit2","doi-asserted-by":"publisher","first-page":"180","DOI":"10.2527\/jam2016-0372","article-title":"Sexed-semen usage\nfor Holstein AI in the United States","volume":"94","author":"Hutchison","year":"2016","journal-title":"J. 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Reprod.\nDev."},{"issue":"s1","key":"2026081415112348000_cit6","doi-asserted-by":"publisher","first-page":"s75","DOI":"10.1017\/S1751731118000952","article-title":"Review:\nSemen handling, time of insemination and insemination technique in\ncattle","volume":"12","author":"Diskin","year":"2018","journal-title":"Animal"},{"issue":"1","key":"2026081415112348000_cit7","doi-asserted-by":"publisher","first-page":"18","DOI":"10.1111\/rda.12640","article-title":"Flow Cytometric Chromosomal Sex Sorting\nof Stallion Spermatozoa Induces Oxidative Stress on Mitochondria and\nGenomic DNA","volume":"51","author":"Balao\nda Silva","year":"2016","journal-title":"Reprod Domest Anim"},{"issue":"E-Supplement 2","key":"2026081415112348000_cit8","first-page":"198","article-title":"Evaluation\nof conception rates of sex-sorted semen\nin commercial dairy farms over the last five years","volume":"100","author":"Heuer","year":"2017","journal-title":"J. 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Pathol."},{"issue":"7","key":"2026081415112348000_cit35","doi-asserted-by":"publisher","first-page":"1305","DOI":"10.1242\/dev.115493","article-title":"Morphogenesis of the mouse neural\nplate depends on distinct roles of cofilin 1 in apical and basal epithelial\ndomains","volume":"142","author":"Grego-Bessa","year":"2015","journal-title":"Development"},{"issue":"2","key":"2026081415112348000_cit36","doi-asserted-by":"publisher","first-page":"649","DOI":"10.1091\/mbc.e04-07-0555","article-title":"Actin-depolymerizing\nfactor and cofilin-1 play overlapping roles in promoting rapid F-actin\ndepolymerization in mammalian nonmuscle cells","volume":"16","author":"Hotulainen","year":"2005","journal-title":"Mol. Biol. 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Neuroendocrinol."},{"issue":"48","key":"2026081415112348000_cit51","doi-asserted-by":"publisher","first-page":"18190","DOI":"10.1073\/pnas.0605274103","article-title":"Evidence for different origin of\nsex chromosomes in snakes, birds, and mammals and step-wise differentiation\nof snake sex chromosomes","volume":"103","author":"Matsubara","year":"2006","journal-title":"Proc. Natl. Acad.\nSci. U. S. A."},{"issue":"4","key":"2026081415112348000_cit52","doi-asserted-by":"publisher","first-page":"205","DOI":"10.1186\/gb-2010-11-4-205","article-title":"Are homologies in\nvertebrate sex determination due\nto shared ancestry or to limited options?","volume":"11","author":"Graves","year":"2010","journal-title":"Genome\nBiology"},{"issue":"D1","key":"2026081415112348000_cit53","doi-asserted-by":"publisher","first-page":"D1211","DOI":"10.1093\/nar\/gky869","article-title":"iProX: an integrated proteome resource","volume":"47","author":"Ma","year":"2019","journal-title":"Nucleic\nAcids Res."}],"container-title":["Journal of Proteome Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acs.jproteome.0c00358","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/jprobs\/article-pdf\/20\/6\/3031\/8429327\/pr0c00358.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/jprobs\/article-pdf\/20\/6\/3031\/8429327\/pr0c00358.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T19:25:53Z","timestamp":1786735553000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/jprobs\/article\/20\/6\/3031\/814857\/Differential-Membrane-Protein-Profile-in-Bovine-X"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,5,19]]},"references-count":53,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2021,5,19]]},"published-print":{"date-parts":[[2021,6,4]]}},"URL":"https:\/\/doi.org\/10.1021\/acs.jproteome.0c00358","relation":{},"ISSN":["1535-3893","1535-3907"],"issn-type":[{"value":"1535-3893","type":"print"},{"value":"1535-3907","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,5,19]]}}}