{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T20:52:51Z","timestamp":1786999971033,"version":"build-2736575974"},"reference-count":12,"publisher":"American Chemical Society (ACS)","issue":"11","license":[{"start":{"date-parts":[[2020,8,10]],"date-time":"2020-08-10T00:00:00Z","timestamp":1597017600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-001"},{"start":{"date-parts":[[2020,8,10]],"date-time":"2020-08-10T00:00:00Z","timestamp":1597017600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-009"},{"start":{"date-parts":[[2020,8,10]],"date-time":"2020-08-10T00:00:00Z","timestamp":1597017600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-017"}],"funder":[{"DOI":"10.13039\/501100012190","name":"Ministry of Science and Higher Education of the Russian Federation","doi-asserted-by":"crossref","award":["075-10-2019-083"],"award-info":[{"award-number":["075-10-2019-083"]}],"id":[{"id":"10.13039\/501100012190","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,11,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The detection of viral RNA by polymerase chain reaction (PCR) is currently the main diagnostic tool for COVID-19 (Eurosurveillance2019, 25 (3), 1). The PCR-based test, however, shows limited sensitivity, especially in the early and late stages of disease development (Nature2020, 581, 465\u2212469; J. Formosan Med. Assoc.2020, 119 (6) 1123), and is relatively time-consuming. Fast and reliable complementary methods for detecting the viral infection would be of help in the current pandemic conditions. Mass spectrometry is one of such possibilities. We have developed a mass-spectrometry-based method for the detection of the SARS CoV-2 virus in nasopharynx epithelial swabs based on the detection of the viral nucleocapsid N protein. Our approach shows confident identification of the N protein in patient samples, even those with the lowest viral loads, and a much simpler preparation procedure. Our main protocol consists of virus inactivation by heating and the addition of isopropanol and tryptic digestion of the proteins sedimented from the swabs followed by MS analysis. A set of unique peptides, produced as a result of proteolysis of the nucleocapsid phosphoprotein of SARS-CoV-2, is detected. The obtained results can further be used to create fast parallel mass-spectrometric approaches for the detection of the virus in the nasopharyngeal mucosa, saliva, sputum and other physiological fluids.<\/jats:p>","DOI":"10.1021\/acs.jproteome.0c00412","type":"journal-article","created":{"date-parts":[[2020,8,10]],"date-time":"2020-08-10T00:03:46Z","timestamp":1597017826000},"page":"4393-4397","source":"Crossref","is-referenced-by-count":92,"title":["Mass-Spectrometric\nDetection of SARS-CoV\u20112\nVirus in Scrapings of the Epithelium of the Nasopharynx of Infected\nPatients via Nucleocapsid N Protein"],"prefix":"10.1021","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6209-2068","authenticated-orcid":true,"given":"Evgeny N.","family":"Nikolaev","sequence":"first","affiliation":[{"name":"Skolkovo Institute of Science and Technology , ,","place":["Moscow, Russia, 143025"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria I.","family":"Indeykina","sequence":"additional","affiliation":[{"name":"Russian Academy of Sciences , , ,","place":["Moscow, Russia, 119334"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander G.","family":"Brzhozovskiy","sequence":"additional","affiliation":[{"name":"Skolkovo Institute of Science and Technology , ,","place":["Moscow, Russia, 143025"]},{"name":"Ministry of Healthcare of the Russian Federation , , ,","place":["Moscow, Russia, 117997"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna E.","family":"Bugrova","sequence":"additional","affiliation":[{"name":"Russian Academy of Sciences , , ,","place":["Moscow, Russia, 119334"]},{"name":"Ministry of Healthcare of the Russian Federation , , ,","place":["Moscow, Russia, 117997"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2238-3458","authenticated-orcid":true,"given":"Alexey S.","family":"Kononikhin","sequence":"additional","affiliation":[{"name":"Skolkovo Institute of Science and Technology , ,","place":["Moscow, Russia, 143025"]},{"name":"Ministry of Healthcare of the Russian Federation , , ,","place":["Moscow, Russia, 117997"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natalia L.","family":"Starodubtseva","sequence":"additional","affiliation":[{"name":"Ministry of Healthcare of the Russian Federation , , ,","place":["Moscow, Russia, 117997"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Evgeny V.","family":"Petrotchenko","sequence":"additional","affiliation":[{"name":"McGill University , , , ,","place":["Montreal, Quebec, Canada, H3T 1E2"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Grigoriy I.","family":"Kovalev","sequence":"additional","affiliation":[{"name":"Skolkovo Institute of Science and Technology , ,","place":["Moscow, Russia, 143025"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2394-6512","authenticated-orcid":true,"given":"Christoph H.","family":"Borchers","sequence":"additional","affiliation":[{"name":"Skolkovo Institute of Science and Technology , ,","place":["Moscow, Russia, 143025"]},{"name":"McGill University , , , ,","place":["Montreal, Quebec, Canada, H3T 1E2"]},{"name":"McGill University , , , ,","place":["Montreal, Quebec, Canada, H3T 1E2"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gennady T.","family":"Sukhikh","sequence":"additional","affiliation":[{"name":"Ministry of Healthcare of the Russian Federation , , ,","place":["Moscow, Russia, 117997"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2020,8,10]]},"reference":[{"issue":"3","key":"2026081714255690600_cit1","doi-asserted-by":"publisher","first-page":"1","DOI":"10.2807\/1560-7917.ES.2020.25.3.2000045","article-title":"Detection\nof 2019 -NCoV by RT-PCR","volume":"25","author":"Corman","year":"2020","journal-title":"Eurosurveillance"},{"key":"2026081714255690600_cit2","doi-asserted-by":"publisher","first-page":"465","DOI":"10.1038\/s41586-020-2196-x","article-title":"Virological\nAssessment of Hospitalized Patients with COVID-2019","volume":"581","author":"Wo\u0308lfel","year":"2020","journal-title":"Nature"},{"issue":"6","key":"2026081714255690600_cit3","doi-asserted-by":"publisher","first-page":"1123","DOI":"10.1016\/j.jfma.2020.04.013","article-title":"Risk of Reactivation\nor Reinfection of Novel Coronavirus (COVID-19)","volume":"119","author":"Alizargar","year":"2020","journal-title":"J. Formosan Med. Assoc."},{"issue":"7798","key":"2026081714255690600_cit4","doi-asserted-by":"publisher","first-page":"265","DOI":"10.1038\/s41586-020-2008-3","article-title":"A New Coronavirus Associated with Human Respiratory\nDisease in China","volume":"579","author":"Wu","year":"2020","journal-title":"Nature"},{"issue":"4","key":"2026081714255690600_cit5","doi-asserted-by":"publisher","first-page":"660","DOI":"10.1128\/CMR.00023-07","article-title":"Severe Acute Respiratory\nSyndrome Coronavirus as an Agent of Emerging and Reemerging Infection","volume":"20","author":"Cheng","year":"2007","journal-title":"Clin. Microbiol. Rev."},{"key":"2026081714255690600_cit6","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jproteome.0c00280","article-title":"Mass Spectrometric Identification\nof SARS-CoV-2 Proteins from Gargle Solution Samples of COVID-19 Patients","author":"Ihling","year":"2020","journal-title":"J. Proteome Res."},{"key":"2026081714255690600_cit7","unstructured":"World Health Organization\n          . Laboratory Biosafety\nGuidance Related to the Novel Coronavirus (2019-nCoV), 2020. https:\/\/www.who.int\/docs\/default-source\/coronaviruse\/laboratory-biosafety-novel-coronavirus-version-1-1.pdf?sfvrsn=912a9847_2 (accessed April 15, 2020)."},{"issue":"10","key":"2026081714255690600_cit8","doi-asserted-by":"publisher","first-page":"1770","DOI":"10.1111\/j.1537-2995.2006.00976.x","article-title":"Evaluation\nof Inactivation Methods for Severe Acute\nRespiratory Syndrome Coronavirus in Noncellular Blood Products","volume":"46","author":"Darnell","year":"2006","journal-title":"Transfusion"},{"issue":"1\u20132","key":"2026081714255690600_cit9","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1007\/s00430-004-0219-0","article-title":"Stability and Inactivation\nof SARS Coronavirus","volume":"194","author":"Rabenau","year":"2005","journal-title":"Med. Microbiol. Immunol."},{"key":"2026081714255690600_cit10","doi-asserted-by":"publisher","DOI":"10.1101\/657908","article-title":"Simplified\nHigh-Throughput Methods for Deep Proteome Analysis on the TimsTOF\nPro","author":"Sandow","year":"2019","journal-title":"bioRxiv"},{"issue":"12","key":"2026081714255690600_cit11","doi-asserted-by":"publisher","first-page":"2534","DOI":"10.1074\/mcp.TIR118.000900","article-title":"Online Parallel Accumulation\u2013Serial\nFragmentation (PASEF) with a Novel Trapped Ion Mobility Mass Spectrometer","volume":"17","author":"Meier","year":"2018","journal-title":"Mol. Cell. 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