{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T20:12:35Z","timestamp":1786738355374,"version":"build-2736575974"},"reference-count":48,"publisher":"American Chemical Society (ACS)","issue":"6","license":[{"start":{"date-parts":[[2021,4,30]],"date-time":"2021-04-30T00:00:00Z","timestamp":1619740800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2021,4,30]],"date-time":"2021-04-30T00:00:00Z","timestamp":1619740800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2021,4,30]],"date-time":"2021-04-30T00:00:00Z","timestamp":1619740800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"funder":[{"DOI":"10.13039\/100000054","name":"National Cancer Institute","doi-asserted-by":"publisher","award":["1R01 CA160254"],"award-info":[{"award-number":["1R01 CA160254"]}],"id":[{"id":"10.13039\/100000054","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000054","name":"National Cancer Institute","doi-asserted-by":"publisher","award":["R50 CA221808"],"award-info":[{"award-number":["R50 CA221808"]}],"id":[{"id":"10.13039\/100000054","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000054","name":"National Cancer Institute","doi-asserted-by":"publisher","award":["U01 CA225753"],"award-info":[{"award-number":["U01 CA225753"]}],"id":[{"id":"10.13039\/100000054","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000054","name":"National Cancer Institute","doi-asserted-by":"publisher","award":["U01 CA230694"],"award-info":[{"award-number":["U01 CA230694"]}],"id":[{"id":"10.13039\/100000054","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007270","name":"University of Michigan","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007270","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,6,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Changes in N-glycosylation on specific peptide sites of serum proteins have been investigated as potential markers for diagnosis of nonalcoholic steatohepatitis (NASH)-related HCC. To accomplish this work, a novel workflow involving broad-scale marker discovery in serum followed by targeted marker evaluation of these glycopeptides were combined. The workflow involved an LC-Stepped HCD-DDA-MS\/MS method coupled with offline peptide fractionation for large-scale identification of N-glycopeptides directly from pooled serum samples (each n = 10) as well as differential determination of N-glycosylation changes between disease states. We then evaluated several potentially diagnostic N-glycopeptides among 78 individual patient samples (40 cirrhosis, 28 early stage NASH HCC, and 10 late-stage NASH HCC) by LC-Stepped HCD-PRM-MS\/MS to quantitatively analyze 65 targeted glycopeptides from 7 glycoproteins. Of these targets, we found site-specific N-glycopeptides n169GSLFAFR_HexNAc\u00ad(4)\u00adHex\u00ad(5)\u00adNeuAc\u00ad(2) and n242ISDGFD\u00adGIPDNV\u00adDAALAL\u00adPAHSY\u00adSGR_Hex\u00adNAc\u00ad(5)\u00adHex\u00ad(6)\u00adFuc\u00ad(1)\u00adNeuAc\u00ad(3) from VTNC were significantly increased comparing samples from patients with NASH cirrhosis and NASH HCC (p &amp;lt; 0.05). When combining results of these 2 glycopeptides with AFP, the ROC curve analysis demonstrated the AUC value increased to 0.834 (95% CI, 0.748\u20130.921) and 0.847 (95% CI, 0.766\u20130.932), respectively, as compared to that of AFP alone (AUC = 0.791, 95% CI, 0.690\u20130.892). These 2 glycopeptides may serve as potential biomarkers for early HCC diagnosis in patients with NASH related cirrhosis.<\/jats:p>","DOI":"10.1021\/acs.jproteome.1c00175","type":"journal-article","created":{"date-parts":[[2021,4,30]],"date-time":"2021-04-30T15:44:55Z","timestamp":1619797495000},"page":"3278-3289","source":"Crossref","is-referenced-by-count":33,"title":["A Panel of Glycopeptides\nas Candidate Biomarkers for\nEarly Diagnosis of NASH Hepatocellular Carcinoma Using a Stepped HCD\nMethod and PRM Evaluation"],"prefix":"10.1021","volume":"20","author":[{"given":"Yu","family":"Lin","sequence":"first","affiliation":[{"name":"University of Michigan Medical Center , , , ,","place":["Ann Arbor, Michigan, United\nStates, 48109"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0051-7777","authenticated-orcid":true,"given":"Jianhui","family":"Zhu","sequence":"additional","affiliation":[{"name":"University of Michigan Medical Center , , , ,","place":["Ann Arbor, Michigan, United\nStates, 48109"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lingyun","family":"Pan","sequence":"additional","affiliation":[{"name":"University of Michigan Medical Center , , , ,","place":["Ann Arbor, Michigan, United\nStates, 48109"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1395-4853","authenticated-orcid":true,"given":"Jie","family":"Zhang","sequence":"additional","affiliation":[{"name":"University of Michigan Medical Center , , , ,","place":["Ann Arbor, Michigan, United\nStates, 48109"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3618-5388","authenticated-orcid":true,"given":"Zhijing","family":"Tan","sequence":"additional","affiliation":[{"name":"University of Michigan Medical Center , , , ,","place":["Ann Arbor, Michigan, United\nStates, 48109"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jocelyn","family":"Olivares","sequence":"additional","affiliation":[{"name":"University of Texas Southwestern Medical Center , , , ,","place":["Dallas, Texas, United States, 75390"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amit G.","family":"Singal","sequence":"additional","affiliation":[{"name":"University of Texas Southwestern Medical Center , , , ,","place":["Dallas, Texas, United States, 75390"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Neehar D.","family":"Parikh","sequence":"additional","affiliation":[{"name":"University of Michigan Medical Center , , , ,","place":["Ann Arbor, Michigan, United States, 48109"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7731-0232","authenticated-orcid":true,"given":"David M.","family":"Lubman","sequence":"additional","affiliation":[{"name":"University of Michigan Medical Center , , , ,","place":["Ann Arbor, Michigan, United\nStates, 48109"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2021,4,30]]},"reference":[{"issue":"10","key":"2026081415121092700_cit1","doi-asserted-by":"publisher","first-page":"589","DOI":"10.1038\/s41575-019-0186-y","article-title":"A global\nview of hepatocellular carcinoma: trends,\nrisk, prevention and management","volume":"16","author":"Yang","year":"2019","journal-title":"Nat. Rev. Gastroenterol.\nHepatol."},{"issue":"4","key":"2026081415121092700_cit2","doi-asserted-by":"publisher","first-page":"e1001624","DOI":"10.1371\/journal.pmed.1001624","article-title":"Early detection, curative\ntreatment,\nand survival rates for hepatocellular carcinoma surveillance in patients\nwith cirrhosis: a meta-analysis","volume":"11","author":"Singal","year":"2014","journal-title":"PLoS Med."},{"issue":"6","key":"2026081415121092700_cit3","doi-asserted-by":"publisher","first-page":"1706","DOI":"10.1053\/j.gastro.2018.01.064","article-title":"Surveillance imaging and alpha fetoprotein\nfor early detection of hepatocellular carcinoma in patients with cirrhosis:\na meta-analysis","volume":"154","author":"Tzartzeva","year":"2018","journal-title":"Gastroenterology"},{"issue":"5","key":"2026081415121092700_cit4","doi-asserted-by":"publisher","first-page":"870","DOI":"10.1016\/j.cgh.2013.09.053","article-title":"Factors that affect accuracy of alpha-fetoprotein\ntest in detection of hepatocellular carcinoma in patients with cirrhosis","volume":"12","author":"Gopal","year":"2014","journal-title":"Clin. Gastroenterol. Hepatol."},{"issue":"1","key":"2026081415121092700_cit5","doi-asserted-by":"publisher","first-page":"5","DOI":"10.1177\/1756283X10385964","article-title":"Surveillance for\nhepatocellular carcinoma: in whom\nand how?","volume":"4","author":"El-Serag","year":"2011","journal-title":"Ther. Adv. Gastroenterol."},{"issue":"12","key":"2026081415121092700_cit6","doi-asserted-by":"publisher","first-page":"2495","DOI":"10.1158\/1055-9965.EPI-20-0005","article-title":"Biomarkers for the\nearly detection of hepatocellular\ncarcinoma","volume":"29","author":"Parikh","year":"2020","journal-title":"Cancer Epidemiol., Biomarkers Prev."},{"issue":"1","key":"2026081415121092700_cit7","doi-asserted-by":"publisher","first-page":"123","DOI":"10.1002\/hep.29466","article-title":"Modeling\nthe epidemic of nonalcoholic fatty liver disease demonstrates an exponential\nincrease in burden of disease","volume":"67","author":"Estes","year":"2018","journal-title":"Hepatology"},{"issue":"5","key":"2026081415121092700_cit8","doi-asserted-by":"publisher","first-page":"855","DOI":"10.1016\/j.cell.2006.08.019","article-title":"Glycosylation in\ncellular mechanisms of health and\ndisease","volume":"126","author":"Ohtsubo","year":"2006","journal-title":"Cell"},{"issue":"13","key":"2026081415121092700_cit9","doi-asserted-by":"publisher","first-page":"6479","DOI":"10.1021\/acs.analchem.5b01679","article-title":"Large-scale measurement\nof absolute protein glycosylation stoichiometry","volume":"87","author":"Sun","year":"2015","journal-title":"Anal. Chem."},{"issue":"4\u20135","key":"2026081415121092700_cit10","doi-asserted-by":"publisher","first-page":"259","DOI":"10.1155\/2008\/264594","article-title":"Fucosylated glycoproteins as markers\nof liver disease","volume":"25","author":"Mehta","year":"2008","journal-title":"Dis. Markers"},{"issue":"5","key":"2026081415121092700_cit11","doi-asserted-by":"publisher","first-page":"1281","DOI":"10.1074\/mcp.M112.023259","article-title":"Site-specific glycoforms of haptoglobin in liver cirrhosis\nand hepatocellular carcinoma","volume":"12","author":"Pompach","year":"2013","journal-title":"Mol. Cell. Proteomics"},{"issue":"5","key":"2026081415121092700_cit12","doi-asserted-by":"publisher","first-page":"1294","DOI":"10.1074\/mcp.M112.023325","article-title":"Quantitative liquid chromatography-mass spectrometry-multiple\nreaction monitoring (LC-MS-MRM) analysis of site-specific glycoforms\nof haptoglobin in liver disease","volume":"12","author":"Sanda","year":"2013","journal-title":"Mol. 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Chem."},{"issue":"6","key":"2026081415121092700_cit17","doi-asserted-by":"publisher","first-page":"651","DOI":"10.1038\/nbt1315","article-title":"Improving\nthe success rate of proteome analysis by\nmodeling protein-abundance distributions and experimental designs","volume":"25","author":"Eriksson","year":"2007","journal-title":"Nat. Biotechnol."},{"issue":"9","key":"2026081415121092700_cit18","doi-asserted-by":"publisher","first-page":"3439","DOI":"10.1021\/acs.jproteome.9b00332","article-title":"Highly efficient\nanalysis of glycoprotein sialylation in human serum by simultaneous\nquantification of glycosites and site-specific glycoforms","volume":"18","author":"Qin","year":"2019","journal-title":"J. Proteome Res."},{"issue":"6","key":"2026081415121092700_cit19","doi-asserted-by":"publisher","first-page":"2887","DOI":"10.1021\/pr500043k","article-title":"Mass-selected site-specific\ncore-fucosylation of ceruloplasmin in alcohol-related hepatocellular\ncarcinoma","volume":"13","author":"Yin","year":"2014","journal-title":"J. Proteome Res."},{"issue":"22","key":"2026081415121092700_cit20","doi-asserted-by":"publisher","first-page":"8802","DOI":"10.1021\/ac201676a","article-title":"Fragmentation\nand site-specific quantification of core fucosylated glycoprotein\nby multiple reaction monitoring-mass spectrometry","volume":"83","author":"Zhao","year":"2011","journal-title":"Anal. Chem."},{"key":"2026081415121092700_cit21","doi-asserted-by":"publisher","first-page":"67","DOI":"10.1016\/j.jprot.2018.02.003","article-title":"Quantitative\nanalysis of core fucosylation of serum proteins in liver diseases\nby LC-MS-MRM","volume":"189","author":"Ma","year":"2018","journal-title":"J. Proteomics"},{"issue":"1","key":"2026081415121092700_cit22","doi-asserted-by":"publisher","first-page":"1311","DOI":"10.1038\/s41467-019-09222-w","article-title":"Capturing site-specific\nheterogeneity with large-scale N-glycoproteome analysis","volume":"10","author":"Riley","year":"2019","journal-title":"Nat. Commun."},{"issue":"9","key":"2026081415121092700_cit23","doi-asserted-by":"publisher","first-page":"2935","DOI":"10.1021\/acs.jproteome.5b01145","article-title":"Multifucosylated\nalpha-1-acid\nglycoprotein as a novel marker for hepatocellular carcinoma","volume":"15","author":"Tanabe","year":"2016","journal-title":"J. Proteome Res."},{"issue":"1","key":"2026081415121092700_cit24","doi-asserted-by":"publisher","first-page":"84","DOI":"10.1038\/nbt.3403","article-title":"Comprehensive analysis\nof protein glycosylation by solid-phase extraction of N-linked glycans\nand glycosite-containing peptides","volume":"34","author":"Sun","year":"2016","journal-title":"Nat. Biotechnol."},{"issue":"7","key":"2026081415121092700_cit25","doi-asserted-by":"publisher","first-page":"2885","DOI":"10.1021\/acs.jproteome.9b00191","article-title":"GPSeeker enables quantitative\nstructural N-glycoproteomics for site-\nand structure-specific characterization of differentially expressed\nN-glycosylation in hepatocellular carcinoma","volume":"18","author":"Xiao","year":"2019","journal-title":"J. Proteome Res."},{"issue":"4","key":"2026081415121092700_cit26","doi-asserted-by":"publisher","first-page":"672","DOI":"10.1074\/mcp.RA119.001791","article-title":"Large-scale identification of N-linked\nintact glycopeptides in human\nserum using HILIC enrichment and spectral library search","volume":"19","author":"Shu","year":"2020","journal-title":"Mol. Cell. Proteomics"},{"issue":"10","key":"2026081415121092700_cit27","doi-asserted-by":"publisher","first-page":"3624","DOI":"10.1021\/acs.jproteome.6b00429","article-title":"HILIC and\nERLIC enrichment of glycopeptides\nderived from breast and brain cancer cells","volume":"15","author":"Zacharias","year":"2016","journal-title":"J. Proteome Res."},{"issue":"2","key":"2026081415121092700_cit28","doi-asserted-by":"publisher","first-page":"651","DOI":"10.1021\/pr8008012","article-title":"Glycoproteomics\nanalysis of human liver tissue by combination of multiple enzyme digestion\nand hydrazide chemistry","volume":"8","author":"Chen","year":"2009","journal-title":"J. Proteome Res."},{"issue":"3","key":"2026081415121092700_cit29","doi-asserted-by":"publisher","first-page":"1713","DOI":"10.1021\/pr401200h","article-title":"Comprehensive\nmapping of protein N-glycosylation in\nhuman liver by combining hdrophilic interation chromatography and\nhydrazide chemistry","volume":"13","author":"Zhu","year":"2014","journal-title":"J. Proteome Res."},{"issue":"1","key":"2026081415121092700_cit30","doi-asserted-by":"publisher","first-page":"267","DOI":"10.1021\/acs.analchem.9b04651","article-title":"Recent advances in glycoproteomic analysis by mass\nspectrometry","volume":"92","author":"Suttapitugsakul","year":"2020","journal-title":"Anal. Chem."},{"issue":"12","key":"2026081415121092700_cit31","doi-asserted-by":"publisher","first-page":"4146","DOI":"10.1021\/acs.jproteome.5b01159","article-title":"Characterization of site-specific\nN-glycopeptide isoforms of alpha-1-acid glycoprotein from an interlaboratory\nstudy using LC-MS\/MS","volume":"15","author":"Lee","year":"2016","journal-title":"J. Proteome Res."},{"issue":"1","key":"2026081415121092700_cit32","doi-asserted-by":"publisher","first-page":"438","DOI":"10.1038\/s41467-017-00535-2","article-title":"pGlyco 2.0 enables\nprecision N-glycoproteomics with comprehensive quality control and\none-step mass spectrometry for intact glycopeptide identification","volume":"8","author":"Liu","year":"2017","journal-title":"Nat. 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Mass Spectrom."},{"issue":"4","key":"2026081415121092700_cit35","doi-asserted-by":"publisher","first-page":"1968","DOI":"10.1021\/acs.jproteome.5b00068","article-title":"Large-scale\nidentification of core-fucosylated glycopeptide sites in pancreatic\ncancer serum using mass spectrometry","volume":"14","author":"Tan","year":"2015","journal-title":"J. Proteome\nRes."},{"issue":"3","key":"2026081415121092700_cit36","doi-asserted-by":"publisher","first-page":"551","DOI":"10.1016\/j.cgh.2018.05.039","article-title":"Racial\nand ethnic\ndifferences in presentation and outcomes of hepatocellular carcinoma","volume":"17","author":"Rich","year":"2019","journal-title":"Clin. Gastroenterol. Hepatol."},{"issue":"4","key":"2026081415121092700_cit37","doi-asserted-by":"publisher","first-page":"701","DOI":"10.1111\/apt.15917","article-title":"Sex disparities\nin presentation and prognosis of 1110\npatients with hepatocellular carcinoma","volume":"52","author":"Rich","year":"2020","journal-title":"Aliment.\nPharmacol. Ther."},{"issue":"4","key":"2026081415121092700_cit38","doi-asserted-by":"publisher","first-page":"322","DOI":"10.6004\/jnccn.2018.7105","article-title":"Comparative analysis\nof nonalcoholic steatohepatitis- versus viral hepatitis- and alcohol-related\nliver disease-related hepatocellular carcinoma","volume":"17","author":"Hester","year":"2019","journal-title":"J. Natl. Compr. Cancer Network"},{"issue":"4","key":"2026081415121092700_cit39","doi-asserted-by":"publisher","first-page":"976","DOI":"10.1053\/j.gastro.2018.11.020","article-title":"Accuracy of the\nliver imaging reporting and data System in computed tomography and\nmagnetic resonance image analysis of hepatocellular carcinoma or overall\nmalignancy-a ystematic review","volume":"156","author":"van\nder Pol","year":"2019","journal-title":"Gastroenterology"},{"issue":"2","key":"2026081415121092700_cit40","doi-asserted-by":"publisher","first-page":"723","DOI":"10.1002\/hep.29913","article-title":"Diagnosis, staging,\nand management of hepatocellular carcinoma: 2018 practice guidance\nby the American association for the study of liver diseases","volume":"68","author":"Marrero","year":"2018","journal-title":"Hepatology"},{"key":"2026081415121092700_cit41","first-page":"8","article-title":"The Concepts of\nPower Analysis","volume-title":"Statistical Power Analysis\nfor the Behavioral Sciences","author":"Cohen","year":"1988"},{"key":"2026081415121092700_cit42","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1007\/978-1-4939-3049-4_12","article-title":"Offline High\npH Reversed-Phase Peptide Fractionation for Deep Phosphoproteome Coverage","volume-title":"Phospho-Proteomics","author":"Batth","year":"2016"},{"issue":"21","key":"2026081415121092700_cit43","doi-asserted-by":"publisher","first-page":"11193","DOI":"10.1021\/acs.analchem.7b03641","article-title":"Comparison\nof enrichment methods\nfor intact N- and O-linked glycopeptides using strong anion exchange\nand hydrophilic interaction liquid chromatography","volume":"89","author":"Yang","year":"2017","journal-title":"Anal. Chem."},{"issue":"12","key":"2026081415121092700_cit44","doi-asserted-by":"publisher","first-page":"1356","DOI":"10.1053\/hupa.2001.29675","article-title":"Vitronectin in the\ncirrhotic liver: an immunomarker of mature fibrosis","volume":"32","author":"Koukoulis","year":"2001","journal-title":"Hum. Pathol."},{"issue":"3","key":"2026081415121092700_cit45","doi-asserted-by":"publisher","first-page":"994","DOI":"10.1111\/j.1432-1033.1995.tb20647.x","article-title":"Structures\nof the N-linked oligosaccharides\non human plasma vitronectin","volume":"230","author":"Ogawa","year":"1995","journal-title":"Eur. J. Biochem."},{"issue":"6","key":"2026081415121092700_cit46","doi-asserted-by":"publisher","first-page":"1412","DOI":"10.1002\/hep.1840200606","article-title":"Distribution of vitronectin in plasma\nand liver tissue: relationship to chronic liver disease","volume":"20","author":"Kobayashi","year":"1994","journal-title":"Hepatology"},{"issue":"30","key":"2026081415121092700_cit47","doi-asserted-by":"publisher","first-page":"7999","DOI":"10.1007\/s00216-014-8226-5","article-title":"In-depth analysis\nof site-specific N-glycosylation in vitronectin from human plasma\nby tandem mass spectrometry with immunoprecipitation","volume":"406","author":"Hwang","year":"2014","journal-title":"Anal. Bioanal. Chem."},{"issue":"5","key":"2026081415121092700_cit48","doi-asserted-by":"publisher","first-page":"2328","DOI":"10.1021\/pr4011519","article-title":"Abundance-ratio-based\nsemiquantitative analysis of site-specific N-linked glycopeptides\npresent in the plasma of hepatocellular carcinoma patients","volume":"13","author":"Lee","year":"2014","journal-title":"J. Proteome Res."}],"container-title":["Journal of Proteome Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acs.jproteome.1c00175","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/jprobs\/article-pdf\/20\/6\/3278\/8429421\/pr1c00175.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/jprobs\/article-pdf\/20\/6\/3278\/8429421\/pr1c00175.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T19:23:18Z","timestamp":1786735398000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/jprobs\/article\/20\/6\/3278\/814862\/A-Panel-of-Glycopeptides-as-Candidate-Biomarkers"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,4,30]]},"references-count":48,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2021,4,30]]},"published-print":{"date-parts":[[2021,6,4]]}},"URL":"https:\/\/doi.org\/10.1021\/acs.jproteome.1c00175","relation":{},"ISSN":["1535-3893","1535-3907"],"issn-type":[{"value":"1535-3893","type":"print"},{"value":"1535-3907","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,4,30]]}}}