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Enriching and quantifying newly synthesized proteins (NSPs) at set time points by mass spectrometry has the potential to identify important early regulatory or expression changes associated with disease states or perturbations. NSP can be enriched from proteomes by employing pulsed introduction of the noncanonical amino acid, azidohomoalanine (AHA). We demonstrate that pulsed introduction of AHA in the feed of mice can label and identify NSP from multiple tissues. Furthermore, we quantitate differences in new protein expression resulting from CRE-LOX initiated knockout of LKB1 in mouse livers. Overall, the PALM strategy allows for the first time in vivo labeling of mouse tissues to differentiate protein synthesis rates at discrete time points.<\/jats:p>","DOI":"10.1021\/acs.jproteome.5b00653","type":"journal-article","created":{"date-parts":[[2015,10,7]],"date-time":"2015-10-07T14:27:18Z","timestamp":1444228038000},"page":"4815-4822","source":"Crossref","is-referenced-by-count":84,"title":["Pulsed Azidohomoalanine\nLabeling in Mammals (PALM)\nDetects Changes in Liver-Specific LKB1 Knockout Mice"],"prefix":"10.1021","volume":"14","author":[{"given":"Daniel B.","family":"McClatchy","sequence":"first","affiliation":[{"name":"The Scripps Research Institute , , 10550 North Torrey Pines Road , , ,","place":["La Jolla, California, United States, 92037"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuanhui","family":"Ma","sequence":"additional","affiliation":[{"name":"The Scripps Research Institute , , 10550 North Torrey Pines Road , , ,","place":["La Jolla, California, United States, 92037"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chao","family":"Liu","sequence":"additional","affiliation":[{"name":"Chinese Academy of Sciences , , No. 6 Kexueyuan South Road , ,","place":["Beijing, China, 100190"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benjamin D.","family":"Stein","sequence":"additional","affiliation":[{"name":"The Scripps Research Institute , , 10550 North Torrey Pines Road , , ,","place":["La Jolla, California, United States, 92037"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Salvador","family":"Marti\u0301nez-Bartolome\u0301","sequence":"additional","affiliation":[{"name":"The Scripps Research Institute , , 10550 North Torrey Pines Road , , ,","place":["La Jolla, California, United States, 92037"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Debbie","family":"Vasquez","sequence":"additional","affiliation":[{"name":"The Salk Institute for Biological Studies , , 10010 North Torrey Pines Road , , ,","place":["La Jolla, California, United States, 92037"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristina","family":"Hellberg","sequence":"additional","affiliation":[{"name":"The Salk Institute for Biological Studies , , 10010 North Torrey Pines Road , , ,","place":["La Jolla, California, United States, 92037"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reuben J.","family":"Shaw","sequence":"additional","affiliation":[{"name":"The Salk Institute for Biological Studies , , 10010 North Torrey Pines Road , , ,","place":["La Jolla, California, United States, 92037"]},{"name":"The Salk Institute for Biological Studies , , 10010 North Torrey Pines Road , , ,","place":["La Jolla, California, United States, 92037"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"III","given":"John R.","family":"Yates","sequence":"additional","affiliation":[{"name":"The Scripps Research Institute , , 10550 North Torrey Pines Road , , ,","place":["La Jolla, California, United States, 92037"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2015,10,7]]},"reference":[{"key":"2026090116340636400_cit1","doi-asserted-by":"publisher","first-page":"41","DOI":"10.1016\/j.neuron.2012.05.024","article-title":"Network organization\nof the huntingtin proteomic interactome in mammalian\nbrain","volume":"75","author":"Shirasaki","year":"2012","journal-title":"Neuron"},{"key":"2026090116340636400_cit2","doi-asserted-by":"publisher","first-page":"704","DOI":"10.1038\/nature06022","article-title":"Global changes to the ubiquitin system in Huntington\u2019s\ndisease","volume":"448","author":"Bennett","year":"2007","journal-title":"Nature"},{"key":"2026090116340636400_cit3","doi-asserted-by":"publisher","first-page":"63","DOI":"10.1038\/nbt.2464","article-title":"Mass-encoded synthetic\nbiomarkers for multiplexed urinary monitoring\nof disease","volume":"31","author":"Kwong","year":"2013","journal-title":"Nat. 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