{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T16:14:37Z","timestamp":1788279277633,"version":"build-2803163510"},"reference-count":38,"publisher":"American Chemical Society (ACS)","issue":"12","license":[{"start":{"date-parts":[[2015,11,24]],"date-time":"2015-11-24T00:00:00Z","timestamp":1448323200000},"content-version":"vor","delay-in-days":19,"URL":"https:\/\/pubs.acs.org\/page\/policy\/authorchoice_ccby_termsofuse.html"}],"funder":[{"DOI":"10.13039\/501100002428","name":"Austrian Science Fund","doi-asserted-by":"publisher","award":["P21946"],"award-info":[{"award-number":["P21946"]}],"id":[{"id":"10.13039\/501100002428","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002428","name":"Austrian Science Fund","doi-asserted-by":"publisher","award":["P23922"],"award-info":[{"award-number":["P23922"]}],"id":[{"id":"10.13039\/501100002428","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2015,12,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Recent studies have shown a remarkable degree of plasticity in the N-glycome of the model nematode Caenorhabditis elegans; ablation of glycosylation-relevant genes can result in radically altered N-glycan profiles despite only minor biological phenotypic effects. Up to four fucose residues and five different linkages of fucose are known on the N-glycans of C. elegans. Due to the complexity in the wild type, we established three mutant strains defective in two core fucosyltransferases each (fut-1;fut-6, fut-1;fut-8, and fut-6;fut-8). Enzymatically released N-glycans were subject to HPLC and MALDI-TOF MS\/MS, in combination with various treatments, to verify structural details. The N-glycome of the fut-1;fut-6 mutant was the most complex of the three double-mutant strains due to the extension of the core \u03b11,6-fucose as well as the presence of fucose on the bisecting galactose. In contrast, maximally two fucoses were found on N-glycans of the fut-1;fut-8 and fut-6;fut-8 strains. The different locations and capping of fucose meant that up to 13 isomeric structures, many highly galactosylated, were determined for some single masses. These data not only show the high variability of the N-glycomic capacity of a \u201csimple\u201d nematode but also exemplify the need for multiple approaches to reveal individual glycan structures within complex invertebrate glycomes.<\/jats:p>","DOI":"10.1021\/acs.jproteome.5b00746","type":"journal-article","created":{"date-parts":[[2015,11,5]],"date-time":"2015-11-05T06:39:32Z","timestamp":1446705572000},"page":"5291-5305","source":"Crossref","is-referenced-by-count":37,"title":["Comparisons of\n                    <i>Caenorhabditis<\/i>\n                    Fucosyltransferase\nMutants Reveal a Multiplicity of Isomeric N-Glycan Structures"],"prefix":"10.1021","volume":"14","author":[{"given":"Shi","family":"Yan","sequence":"first","affiliation":[{"name":"Universita\u0308t fu\u0308r Bodenkultur , , ,","place":["Wien, Austria, 1190"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunsheng","family":"Jin","sequence":"additional","affiliation":[{"name":"Go\u0308teborgs Universitet , , ,","place":["Go\u0308teborg, Sweden, 405 30"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iain B. H.","family":"Wilson","sequence":"additional","affiliation":[{"name":"Universita\u0308t fu\u0308r Bodenkultur , , ,","place":["Wien, Austria, 1190"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katharina","family":"Paschinger","sequence":"additional","affiliation":[{"name":"Universita\u0308t fu\u0308r Bodenkultur , , ,","place":["Wien, Austria, 1190"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2015,11,5]]},"reference":[{"key":"2026090111102887900_cit1","doi-asserted-by":"publisher","first-page":"661","DOI":"10.1515\/hsz-2012-0150","article-title":"Complicated N-linked glycans in simple organisms","volume":"393","author":"Schiller","year":"2012","journal-title":"Biol. 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Proteome Res."},{"key":"2026090111102887900_cit5","doi-asserted-by":"publisher","first-page":"9127","DOI":"10.1074\/jbc.M606711200","article-title":"Dynamic developmental elaboration of N-linked glycan\ncomplexity in the Drosophila melanogaster embryo","volume":"282","author":"Aoki","year":"2007","journal-title":"J. Biol. Chem."},{"key":"2026090111102887900_cit6","doi-asserted-by":"publisher","first-page":"172","DOI":"10.1016\/j.jprot.2015.05.030","article-title":"Targetted release and fractionation\nreveal glucuronylated and sulphated N- and O-glycans in larvae of\ndipteran insects","volume":"126","author":"Kurz","year":"2015","journal-title":"J. Proteomics"},{"key":"2026090111102887900_cit7","doi-asserted-by":"publisher","first-page":"807","DOI":"10.1093\/oxfordjournals.jbchem.a003169","article-title":"Structure\nAnalysis of N-Linked Glycans\nin Caenorhabditis elegans","volume":"131","author":"Natsuka","year":"2002","journal-title":"J. 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Biotechnol."},{"key":"2026090111102887900_cit29","doi-asserted-by":"publisher","first-page":"448","DOI":"10.1093\/glycob\/cwu132","article-title":"Enzymatic\nproperties and subtle differences in the substrate specificity of\nphylogenetically distinct invertebrate N-glycan processing hexosaminidases","volume":"25","author":"Dragosits","year":"2015","journal-title":"Glycobiology"},{"key":"2026090111102887900_cit30","doi-asserted-by":"publisher","first-page":"19","DOI":"10.1093\/glycob\/5.1.19","article-title":"Purification and characterization of novel glycosidases\nfrom the bacterial genus Xanthomonas","volume":"5","author":"Wong-Madden","year":"1995","journal-title":"Glycobiology"},{"key":"2026090111102887900_cit31","doi-asserted-by":"publisher","first-page":"585","DOI":"10.1093\/glycob\/cwv015","article-title":"Two types of galactosylated fucose motifs are present\non N-glycans of Haemonchus contortus","volume":"25","author":"Paschinger","year":"2015","journal-title":"Glycobiology"},{"key":"2026090111102887900_cit32","doi-asserted-by":"publisher","first-page":"1314","DOI":"10.1002\/elps.201400528","article-title":"Comparison of RP-HPLC modes to analyse\nthe N-glycome of the free-living nematode Pristionchus pacificus","volume":"36","author":"Yan","year":"2015","journal-title":"Electrophoresis"},{"key":"2026090111102887900_cit33","doi-asserted-by":"publisher","first-page":"1747","DOI":"10.1002\/rcm.2509","article-title":"Mass spectrometry\nof proton adducts of fucosylated N-glycans: fucose transfer between\nantennae gives rise to misleading fragments","volume":"20","author":"Wuhrer","year":"2006","journal-title":"Rapid Commun. Mass Spectrom."},{"key":"2026090111102887900_cit34","doi-asserted-by":"publisher","first-page":"73","DOI":"10.1016\/0003-2697(88)90126-1","article-title":"Analyses of N-linked oligosaccharides using a two-dimensional\nmapping technique","volume":"171","author":"Tomiya","year":"1988","journal-title":"Anal. Biochem."},{"key":"2026090111102887900_cit35","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-015-9154-8","article-title":"Sweet secrets\nof a therapeutic worm: Mass spectrometric\nN-glycomic analysis of Trichuris suis","author":"Wilson","year":"2015","journal-title":"Anal. Bioanal. Chem."},{"key":"2026090111102887900_cit36","doi-asserted-by":"publisher","first-page":"163","DOI":"10.1139\/o86-026","article-title":"Biosynthetic\ncontrols that determine the branching and microheterogeneity of protein-bound\noligosaccharides","volume":"64","author":"Schachter","year":"1986","journal-title":"Biochem. Cell Biol."},{"key":"2026090111102887900_cit37","doi-asserted-by":"publisher","first-page":"133","DOI":"10.1016\/j.ab.2008.12.005","article-title":"Specificity analysis\nof lectins and antibodies using remodeled glycoproteins","volume":"386","author":"Iskratsch","year":"2009","journal-title":"Anal. Biochem."},{"key":"2026090111102887900_cit38","doi-asserted-by":"publisher","first-page":"425","DOI":"10.1186\/s13071-015-1032-x","article-title":"Inhibition\nof Haemonchus contortus larval development by fungal\nlectins","volume":"8","author":"Heim","year":"2015","journal-title":"Parasites Vectors"}],"container-title":["Journal of Proteome Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acs.jproteome.5b00746","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/jprobs\/article-pdf\/14\/12\/5291\/67463264\/acs.jproteome.5b00746.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/jprobs\/article-pdf\/14\/12\/5291\/67463264\/acs.jproteome.5b00746.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T15:15:47Z","timestamp":1788275747000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/jprobs\/article\/14\/12\/5291\/1316986\/Comparisons-of-Caenorhabditis-Fucosyltransferase"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,11,5]]},"references-count":38,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2015,11,5]]},"published-print":{"date-parts":[[2015,12,4]]}},"URL":"https:\/\/doi.org\/10.1021\/acs.jproteome.5b00746","relation":{},"ISSN":["1535-3893","1535-3907"],"issn-type":[{"value":"1535-3893","type":"print"},{"value":"1535-3907","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,11,5]]}}}