{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T14:29:50Z","timestamp":1788532190314,"version":"build-2803163510"},"reference-count":48,"publisher":"American Chemical Society (ACS)","issue":"9","license":[{"start":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T00:00:00Z","timestamp":1787097600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000928","name":"Welch Foundation","doi-asserted-by":"publisher","award":["D-0005"],"award-info":[{"award-number":["D-0005"]}],"id":[{"id":"10.13039\/100000928","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100001027","name":"CH Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100001027","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["1R01GM112490-10"],"award-info":[{"award-number":["1R01GM112490-10"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["1R01GM130091-06"],"award-info":[{"award-number":["1R01GM130091-06"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,9,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Traumatic brain injury (TBI) triggers complex neuroinflammatory cascades that involve sustained immune activation and dysregulated antibody effector functions. Immunoglobulin G (IgG) Fc N-glycosylation, particularly core fucosylation, critically modulates immune signaling through altered Fc\u03b3 receptor (Fc\u03b3R) interactions; however, its role in TBI remains unexplored. Here, we developed a high-sensitivity, mass spectrometry-based glycoproteomics method for the systematic analysis of IgG Fc core fucosylation dynamics following TBI. The approach integrates Fc-specific enzymatic truncation with GlycINATOR (EndoS2) and tryptic digestion, followed by high-resolution LC\u2013MS\/MS profiling, enabling confident identification of truncated Fc glycopeptides. Furthermore, a targeted parallel reaction monitoring (PRM) strategy allowed direct quantification of core fucosylated and afucosylated glycopeptides from 10 \u03bcg of crude serum protein, eliminating the need for IgG purification. Our results reveal time-dependent and subclass-specific remodeling of IgG Fc fucosylation postinjury, characterized by an overall reduction in fucosylated species and a relative increase in afucosylation. Collectively, this study establishes a scalable analytical platform for Fc-specific glycosylation profiling and identifies IgG core fucosylation as a candidate molecular indicator of immune dysregulation in TBI, providing new insights into post-traumatic immune regulation.<\/jats:p>","DOI":"10.1021\/acs.jproteome.5c01154","type":"journal-article","created":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T00:01:16Z","timestamp":1787184076000},"page":"4470-4484","source":"Crossref","is-referenced-by-count":0,"title":["Development\nof a High-Sensitivity Glycoproteomics\nApproach for Fc-Specific Quantification of IgG Core Fucosylation in\nTraumatic Brain Injury"],"prefix":"10.1021","volume":"25","author":[{"given":"Sherifdeen","family":"Onigbinde","sequence":"first","affiliation":[{"name":"Texas Tech University , , , ,","place":["Lubbock, Texas, United States, 79409"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joy","family":"Solomon","sequence":"additional","affiliation":[{"name":"Texas Tech University , , , ,","place":["Lubbock, Texas, United States, 79409"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vishal","family":"Sandilya","sequence":"additional","affiliation":[{"name":"Texas Tech University , , , ,","place":["Lubbock, Texas, United States, 79409"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oluwatosin","family":"Daramola","sequence":"additional","affiliation":[{"name":"Texas Tech University , , , ,","place":["Lubbock, Texas, United States, 79409"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mojibola","family":"Fowowe","sequence":"additional","affiliation":[{"name":"Texas Tech University , , , ,","place":["Lubbock, Texas, United States, 79409"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Moyinoluwa","family":"Adeniyi","sequence":"additional","affiliation":[{"name":"Texas Tech University , , , ,","place":["Lubbock, Texas, United States, 79409"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5008-6944","authenticated-orcid":true,"given":"Firas","family":"Kobeissy","sequence":"additional","affiliation":[{"name":"Centre for Neurotrauma, MultiOmics & Biomarkers (CNMB) Morehouse School of Medicine , , ,","place":["Atlanta, Georgia, United States, 30310-145"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefania","family":"Mondello","sequence":"additional","affiliation":[{"name":"University of Messina , , ,","place":["Messina, Italy, 98122"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ava M.","family":"Puccio","sequence":"additional","affiliation":[{"name":"University of Pittsburgh , , , PA ,","place":["Pittsburgh, Pennsylvania, United States, 15213"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6661-6073","authenticated-orcid":true,"given":"Yehia","family":"Mechref","sequence":"additional","affiliation":[{"name":"Texas Tech University , , , ,","place":["Lubbock, Texas, United States, 79409"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,8,19]]},"reference":[{"issue":"11","key":"2026090409130810800_cit1","doi-asserted-by":"publisher","first-page":"1984","DOI":"10.2174\/1570159X19666210215123914","article-title":"The Complexity\nof Secondary Cascade Consequent to Traumatic\nBrain Injury: Pathobiology and Potential Treatments","volume":"19","author":"Khatri","year":"2021","journal-title":"Curr. Neuropharmacol."},{"issue":"16","key":"2026090409130810800_cit2","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2024.e35869","article-title":"Microglia-induced\nneuroinflammation in hippocampal\nneurogenesis following traumatic brain injury","volume":"10","author":"Navabi","year":"2024","journal-title":"Heliyon"},{"issue":"4","key":"2026090409130810800_cit3","doi-asserted-by":"publisher","first-page":"1080","DOI":"10.3171\/2017.10.JNS17352","article-title":"Estimating\nthe global\nincidence of traumatic brain injury","volume":"130","author":"Dewan","year":"2019","journal-title":"J. Neurosurg."},{"issue":"11","key":"2026090409130810800_cit4","doi-asserted-by":"publisher","first-page":"1004","DOI":"10.1016\/S1474-4422(22)00309-X","article-title":"Traumatic\nbrain injury: progress and challenges\nin prevention, clinical care, and research","volume":"21","author":"Maas","year":"2022","journal-title":"Lancet Neurol."},{"issue":"23","key":"2026090409130810800_cit5","doi-asserted-by":"publisher","first-page":"3235","DOI":"10.1089\/neu.2021.0062","article-title":"Epidemiology of chronic effects of\ntraumatic brain injury","volume":"38","author":"Haarbauer-Krupa","year":"2021","journal-title":"J. Neurotrauma"},{"issue":"1","key":"2026090409130810800_cit6","doi-asserted-by":"publisher","first-page":"68","DOI":"10.1186\/s13049-022-01050-0","article-title":"Moderate and severe\ntraumatic brain injury in general hospitals: a ten-year population-based\nretrospective cohort study in central Norway","volume":"30","author":"Rahim","year":"2022","journal-title":"Scand. J. Trauma Resusc. Emerg. Med."},{"issue":"1","key":"2026090409130810800_cit7","doi-asserted-by":"publisher","first-page":"45","DOI":"10.1186\/s40478-020-00924-7","article-title":"Biological\nlinks\nbetween traumatic brain injury and Parkinson\u2019s disease","volume":"8","author":"Delic","year":"2020","journal-title":"Acta Neuropathol. Commun."},{"issue":"9","key":"2026090409130810800_cit8","doi-asserted-by":"publisher","first-page":"1062","DOI":"10.1001\/jamaneurol.2016.1948","article-title":"Association of Traumatic\nBrain Injury With Late-Life Neurodegenerative Conditions and Neuropathologic\nFindings","volume":"73","author":"Crane","year":"2016","journal-title":"JAMA Neurology"},{"issue":"13","key":"2026090409130810800_cit9","doi-asserted-by":"publisher","first-page":"1129","DOI":"10.1089\/neu.2013.3303","article-title":"Linking traumatic\nbrain injury to chronic traumatic\nencephalopathy: identification of potential mechanisms leading to\nneurofibrillary tangle development","volume":"31","author":"Lucke-Wold","year":"2014","journal-title":"J. Neurotrauma"},{"issue":"9","key":"2026090409130810800_cit10","doi-asserted-by":"publisher","first-page":"1725","DOI":"10.1007\/s12031-021-01841-7","article-title":"Traumatic brain injury:\nmechanistic insight on pathophysiology and potential therapeutic targets","volume":"71","author":"Thapa","year":"2021","journal-title":"J. Mol. Neurosci."},{"key":"2026090409130810800_cit11","doi-asserted-by":"publisher","first-page":"740939","DOI":"10.3389\/fphys.2021.740939","article-title":"Traumatic brain injury:\nmechanisms\nof glial response","volume":"12","author":"Mira","year":"2021","journal-title":"Front. Physiol."},{"issue":"5","key":"2026090409130810800_cit12","doi-asserted-by":"publisher","first-page":"1009","DOI":"10.3390\/cells10051009","article-title":"Traumatic brain injury: Ultrastructural features in neuronal ferroptosis,\nglial cell activation and polarization, and blood\u2013brain barrier\nbreakdown","volume":"10","author":"Qin","year":"2021","journal-title":"Cells"},{"issue":"4","key":"2026090409130810800_cit13","doi-asserted-by":"publisher","first-page":"612","DOI":"10.1111\/fcp.12767","article-title":"Oxidative stress and\nmitochondrial dysfunction following traumatic brain injury: From mechanistic\nview to targeted therapeutic opportunities","volume":"36","author":"Hakiminia","year":"2022","journal-title":"Fundam. Clin. Pharmacol."},{"issue":"1\u20132","key":"2026090409130810800_cit14","doi-asserted-by":"publisher","first-page":"67","DOI":"10.1080\/14737159.2024.2306876","article-title":"The game\nchanger: UCH-L1 and GFAP-based blood test as the first marketed in\nvitro diagnostic test for mild traumatic brain injury","volume":"24","author":"Kobeissy","year":"2024","journal-title":"Expert Rev. Mol. Diagn."},{"issue":"11\u201312","key":"2026090409130810800_cit15","doi-asserted-by":"publisher","first-page":"1353","DOI":"10.1089\/neu.2023.0186","article-title":"Diagnostic Utility of\nGlial Fibrillary Acidic Protein Beyond 12 h After Traumatic Brain\nInjury: A TRACK-TBI Study","volume":"41","author":"Puccio","year":"2024","journal-title":"J. Neurotrauma"},{"key":"2026090409130810800_cit16","doi-asserted-by":"publisher","first-page":"1088827","DOI":"10.3389\/fimmu.2022.1088827","article-title":"Neuroinflammation\nof traumatic brain\ninjury: Roles of extracellular vesicles","volume":"13","author":"Liu","year":"2023","journal-title":"Front.\nImmunol."},{"key":"2026090409130810800_cit17","doi-asserted-by":"publisher","first-page":"111066","DOI":"10.1016\/j.brainresbull.2024.111066","article-title":"Neuroimmune and neuroinflammation\nresponse for traumatic brain injury","volume":"217","author":"Wu","year":"2024","journal-title":"Brain Res.\nBull."},{"issue":"16","key":"2026090409130810800_cit18","doi-asserted-by":"publisher","first-page":"3644","DOI":"10.7150\/ijms.46834","article-title":"The peripheral\nimmune system and traumatic brain injury:\nInsight into the role of T-helper cells","volume":"18","author":"Bao","year":"2021","journal-title":"Int.\nJ. Med. Sci."},{"key":"2026090409130810800_cit19","doi-asserted-by":"publisher","first-page":"63","DOI":"10.1007\/82_2019_152","article-title":"IgG Fc\nGlycosylation in Human Immunity","volume":"423","author":"Wang","year":"2019","journal-title":"Curr. Top.\nMicrobiol. Immunol."},{"issue":"9","key":"2026090409130810800_cit20","doi-asserted-by":"publisher","first-page":"3492","DOI":"10.1172\/JCI130029","article-title":"Functional diversification\nof IgGs through Fc glycosylation","volume":"129","author":"Wang","year":"2019","journal-title":"J. Clin. Invest."},{"key":"2026090409130810800_cit21","doi-asserted-by":"publisher","first-page":"929895","DOI":"10.3389\/fimmu.2022.929895","article-title":"Role of Fc\ncore fucosylation in the\neffector function of IgG1 antibodies","volume":"13","author":"Golay","year":"2022","journal-title":"Front.\nImmunol."},{"key":"2026090409130810800_cit22","first-page":"1953220","article-title":"The therapeutic\npotential of sialylated Fc domains of human IgG","volume-title":"Mabs","author":"Pleass","year":"2021"},{"key":"2026090409130810800_cit23","doi-asserted-by":"publisher","first-page":"877","DOI":"10.3389\/fimmu.2017.00877","article-title":"Decoding\nthe human immunoglobulin G-glycan repertoire reveals a spectrum of\nFc-receptor-and complement-mediated-effector activities","volume":"8","author":"Dekkers","year":"2017","journal-title":"Front. Immunol."},{"issue":"6","key":"2026090409130810800_cit24","doi-asserted-by":"publisher","first-page":"2390","DOI":"10.1182\/blood-2008-03-144600","article-title":"Antibody fucosylation differentially impacts cytotoxicity\nmediated by NK and PMN effector cells","volume":"112","author":"Peipp","year":"2008","journal-title":"Blood"},{"issue":"3","key":"2026090409130810800_cit25","doi-asserted-by":"publisher","first-page":"326","DOI":"10.4161\/mabs.19941","article-title":"Quantitative evaluation\nof fucose reducing effects\nin a humanized antibody on Fc\u03b3 receptor binding and antibody-dependent\ncell-mediated cytotoxicity activities","volume":"4","author":"Chung","year":"2012","journal-title":"mAbs"},{"issue":"5","key":"2026090409130810800_cit26","doi-asserted-by":"publisher","first-page":"774","DOI":"10.1074\/mcp.RA119.001860","article-title":"Decreased Immunoglobulin G Core Fucosylation, A Player in Antibody-dependent\nCell-mediated Cytotoxicity, is Associated with Autoimmune Thyroid\nDiseases","volume":"19","author":"Martin","year":"2020","journal-title":"Mol. Cell. Proteomics"},{"key":"2026090409130810800_cit27","doi-asserted-by":"publisher","first-page":"103883","DOI":"10.1016\/j.ebiom.2022.103883","article-title":"Fucosylation of anti-dsDNA IgG1 correlates\nwith disease\nactivity of treatment-nai\u0308ve systemic lupus erythematosus patients","volume":"77","author":"Han","year":"2022","journal-title":"EBioMedicine"},{"issue":"12","key":"2026090409130810800_cit28","doi-asserted-by":"publisher","first-page":"105365","DOI":"10.1016\/j.jbc.2023.105365","article-title":"A highly\nspecific antibody against the core fucose of the N-glycan\nin IgG identifies the pulmonary diseases and its regulation by CCL2","volume":"299","author":"Kanto","year":"2023","journal-title":"J. Biol. Chem."},{"issue":"12","key":"2026090409130810800_cit29","doi-asserted-by":"publisher","first-page":"3078","DOI":"10.1158\/1078-0432.CCR-15-1867","article-title":"IgG Glycome\nin Colorectal Cancer","volume":"22","author":"Vuc\u030ckovic\u0301","year":"2016","journal-title":"Clin. Cancer Res."},{"issue":"596","key":"2026090409130810800_cit30","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.abf8654","article-title":"High titers and low\nfucosylation of early human anti\u2013SARS-CoV-2 IgG promote inflammation\nby alveolar macrophages","volume":"13","author":"Hoepel","year":"2021","journal-title":"Sci. Transl. Med."},{"issue":"6532","key":"2026090409130810800_cit31","doi-asserted-by":"publisher","DOI":"10.1126\/science.abc8378","article-title":"Afucosylated IgG characterizes enveloped viral\nresponses and correlates with COVID-19 severity","volume":"371","author":"Larsen","year":"2021","journal-title":"Science"},{"issue":"1","key":"2026090409130810800_cit32","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1038\/s41514-021-00055-w","article-title":"Association of dementia with immunoglobulin G N-glycans in a Chinese\nHan Population","volume":"7","author":"Zhang","year":"2021","journal-title":"NPJ. Aging Mech Dis"},{"issue":"2","key":"2026090409130810800_cit33","doi-asserted-by":"publisher","first-page":"821","DOI":"10.1021\/pr300887z","article-title":"High-throughput\nIgG\nFc N-glycosylation profiling by mass spectrometry of glycopeptides","volume":"12","author":"Bakovic\u0301","year":"2013","journal-title":"J. Proteome Res."},{"key":"2026090409130810800_cit34","doi-asserted-by":"publisher","first-page":"342907","DOI":"10.1016\/j.aca.2024.342907","article-title":"LC-MS\/MS of isomeric N-and O-glycopeptides\non mesoporous graphitized carbon column","volume":"1317","author":"Daramola","year":"2024","journal-title":"Anal.\nChim. Acta"},{"issue":"2","key":"2026090409130810800_cit35","doi-asserted-by":"publisher","first-page":"1528","DOI":"10.1021\/acs.analchem.5c06005","article-title":"GlypPRM: An Automated\nAnalyzer and Quantification Tool for Glycopeptides Parallel Reaction\nMonitoring","volume":"98","author":"Daramola","year":"2026","journal-title":"Anal. Chem."},{"issue":"6323","key":"2026090409130810800_cit36","doi-asserted-by":"publisher","first-page":"395","DOI":"10.1126\/science.aai8128","article-title":"IgG antibodies to dengue\nenhanced for Fc\u03b3RIIIA binding determine disease severity","volume":"355","author":"Wang","year":"2017","journal-title":"Science"},{"issue":"1","key":"2026090409130810800_cit37","doi-asserted-by":"publisher","first-page":"67","DOI":"10.1038\/s41590-020-00828-7","article-title":"Proinflammatory IgG\nFc structures in patients with severe COVID-19","volume":"22","author":"Chakraborty","year":"2021","journal-title":"Nat. Immunol."},{"issue":"596","key":"2026090409130810800_cit38","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.abf8654","article-title":"High titers and low\nfucosylation of early human anti-SARS-CoV-2 IgG promote inflammation\nby alveolar macrophages","volume":"13","author":"Hoepel","year":"2021","journal-title":"Sci. Transl. Med."},{"issue":"19","key":"2026090409130810800_cit39","doi-asserted-by":"publisher","first-page":"9626","DOI":"10.3390\/ijms26199626","article-title":"Immunoglobulin G Subclass-Specific\nGlycosylation Changes in Rheumatoid Arthritis","volume":"26","author":"Szabo\u0301","year":"2025","journal-title":"Int. J. Mol. Sci."},{"key":"2026090409130810800_cit40","doi-asserted-by":"publisher","first-page":"654","DOI":"10.3389\/fimmu.2020.00654","article-title":"Immunoglobulin\nG Subclass-Specific Glycosylation Changes in Primary Epithelial Ovarian\nCancer","volume":"11","author":"Wieczorek","year":"2020","journal-title":"Front. Immunol."},{"key":"2026090409130810800_cit41","doi-asserted-by":"publisher","first-page":"103883","DOI":"10.1016\/j.ebiom.2022.103883","article-title":"Fucosylation of anti-dsDNA IgG1 correlates with disease\nactivity of treatment-nai\u0308ve systemic lupus erythematosus patients","volume":"77","author":"Han","year":"2022","journal-title":"EBioMedicine"},{"issue":"6","key":"2026090409130810800_cit42","doi-asserted-by":"publisher","first-page":"107642","DOI":"10.1016\/j.celrep.2020.107642","article-title":"Maternal Anti-Dengue IgG Fucosylation Predicts Susceptibility to\nDengue Disease in Infants","volume":"31","author":"Thulin","year":"2020","journal-title":"Cell Rep."},{"key":"2026090409130810800_cit43","doi-asserted-by":"publisher","first-page":"137","DOI":"10.1016\/j.bbi.2022.02.017","article-title":"Subacute\ncytokine changes after a traumatic brain injury predict chronic brain\nmicrostructural alterations on advanced diffusion imaging in the male\nrat","volume":"102","author":"Vinh To","year":"2022","journal-title":"Brain, Behav., Immun."},{"issue":"1","key":"2026090409130810800_cit44","doi-asserted-by":"publisher","first-page":"52","DOI":"10.1186\/s12974-025-03365-y","article-title":"Inhibition\nof IL-6 trans-signaling promotes post-stroke functional recovery in\na sex and dose-dependent manner","volume":"22","author":"Hall","year":"2025","journal-title":"J. Neuroinflammation"},{"key":"2026090409130810800_cit45","doi-asserted-by":"publisher","first-page":"56","DOI":"10.1016\/j.bbi.2023.06.027","article-title":"Associations\nof neuroinflammatory\nIL-6 and IL-8 with brain atrophy, memory decline, and core AD biomarkers\u2013in\ncognitively unimpaired older adults","volume":"113","author":"Capogna","year":"2023","journal-title":"Brain,\nBehav., Immun."},{"issue":"5","key":"2026090409130810800_cit46","doi-asserted-by":"publisher","first-page":"328","DOI":"10.1038\/nri2762","article-title":"FcgammaRIIB\nin autoimmunity and infection: evolutionary\nand therapeutic implications","volume":"10","author":"Smith","year":"2010","journal-title":"Nat. Rev. Immunol."},{"issue":"3","key":"2026090409130810800_cit47","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0092698","article-title":"Human traumatic brain injury induces autoantibody\nresponse against glial fibrillary acidic protein and its breakdown\nproducts","volume":"9","author":"Zhang","year":"2014","journal-title":"PLoS One"},{"key":"2026090409130810800_cit48","doi-asserted-by":"crossref","first-page":"431","DOI":"10.1016\/B978-0-12-816346-7.00029-4","article-title":"Chapter 29 -\nAutoantibodies in central nervous system trauma: new frontiers for\ndiagnosis and prognosis biomarkers","volume-title":"Biomarkers\nfor Traumatic Brain Injury","author":"Kobeissy","year":"2020"}],"container-title":["Journal of Proteome Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acs.jproteome.5c01154","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/jprobs\/article-pdf\/25\/9\/4470\/66886800\/acs.jproteome.5c01154.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/jprobs\/article-pdf\/25\/9\/4470\/66886800\/acs.jproteome.5c01154.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T14:11:22Z","timestamp":1788531082000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/jprobs\/article\/25\/9\/4470\/5277210\/Development-of-a-High-Sensitivity-Glycoproteomics"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,19]]},"references-count":48,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2026,8,19]]},"published-print":{"date-parts":[[2026,9,4]]}},"URL":"https:\/\/doi.org\/10.1021\/acs.jproteome.5c01154","relation":{},"ISSN":["1535-3893","1535-3907"],"issn-type":[{"value":"1535-3893","type":"print"},{"value":"1535-3907","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,19]]}}}