{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T19:56:32Z","timestamp":1790538992056,"version":"4.1.0"},"reference-count":31,"publisher":"American Chemical Society (ACS)","issue":"12","license":[{"start":{"date-parts":[[2016,10,27]],"date-time":"2016-10-27T00:00:00Z","timestamp":1477526400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2016,10,27]],"date-time":"2016-10-27T00:00:00Z","timestamp":1477526400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2016,10,27]],"date-time":"2016-10-27T00:00:00Z","timestamp":1477526400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"funder":[{"DOI":"10.13039\/501100000272","name":"Department of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000272","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002141","name":"Public Health England","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002141","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2016,12,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Ebola virus (EBOV) infection results in severe disease and in some cases lethal hemorrhagic fever. The infection is directed by seven viral genes that encode nine viral proteins. By definition, viruses are obligate intracellular parasites and require aspects of host cell biology in order to replicate their genetic material, assemble new virus particles, and subvert host cell antiviral responses. Currently licensed antivirals are targeted against viral proteins to inhibit their function. However, experience with treating HIV and influenza virus demonstrates that resistant viruses are soon selected. An emerging area in virology is to transiently target host cell proteins that play critical proviral roles in virus biology, especially for acute infections. This has the advantage that the protein being targeted is evolutionary removed from the genome of the virus. Proteomics can aid in discovery biology and identify cellular proteins that may be utilized by the virus to facilitate infection. This work focused on defining the interactome of the EBOV nucleoprotein and identified that cellular chaperones, including HSP70, associate with this protein to promote stability. Utilization of a mini-genome replication system based on a recent Makona isolate demonstrated that disrupting the stability of NP had an adverse effect on viral RNA synthesis.<\/jats:p>","DOI":"10.1021\/acs.jproteome.6b00337","type":"journal-article","created":{"date-parts":[[2016,10,27]],"date-time":"2016-10-27T09:55:13Z","timestamp":1477562113000},"page":"4290-4303","source":"Crossref","is-referenced-by-count":55,"title":["Elucidation of\nthe Cellular Interactome of Ebola Virus\nNucleoprotein and Identification of Therapeutic Targets"],"prefix":"10.1021","volume":"15","author":[{"given":"Isabel","family":"Garci\u0301a-Dorival","sequence":"first","affiliation":[{"name":"University of Liverpool , , ,","place":["Liverpool, U.K., L3 5RF"]},{"name":"NIHR Health Protection Research Unit in Emerging and Zoonotic Infections , ,","place":["Liverpool, U.K., L69 3GL"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weining","family":"Wu","sequence":"additional","affiliation":[{"name":"University of Liverpool , , ,","place":["Liverpool, U.K., L3 5RF"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stuart D.","family":"Armstrong","sequence":"additional","affiliation":[{"name":"University of Liverpool , , ,","place":["Liverpool, U.K., L3 5RF"]},{"name":"NIHR Health Protection Research Unit in Emerging and Zoonotic Infections , ,","place":["Liverpool, U.K., L69 3GL"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John N.","family":"Barr","sequence":"additional","affiliation":[{"name":"University of Leeds , , ,","place":["Leeds, U.K., LS2 9JT"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miles\nW.","family":"Carroll","sequence":"additional","affiliation":[{"name":"NIHR Health Protection Research Unit in Emerging and Zoonotic Infections , ,","place":["Liverpool, U.K., L69 3GL"]},{"name":"Public Health England , Porton Down, ,","place":["Salisbury, U.K., SP4 0JG"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roger","family":"Hewson","sequence":"additional","affiliation":[{"name":"Public Health England , Porton Down, ,","place":["Salisbury, U.K., SP4 0JG"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julian A.","family":"Hiscox","sequence":"additional","affiliation":[{"name":"University of Liverpool , , ,","place":["Liverpool, U.K., L3 5RF"]},{"name":"NIHR Health Protection Research Unit in Emerging and Zoonotic Infections , ,","place":["Liverpool, U.K., L69 3GL"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2016,10,27]]},"reference":[{"issue":"2","key":"2026081810431862500_cit1","doi-asserted-by":"publisher","first-page":"917","DOI":"10.1128\/JVI.01783-14","article-title":"Interactome Analysis\nof the Human Respiratory Syncytial Virus RNA\nPolymerase Complex Identifies Protein Chaperones as Important Cofactors\nThat Promote L-Protein Stability and RNA Synthesis","volume":"89","author":"Munday","year":"2015","journal-title":"J. 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