{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T16:52:01Z","timestamp":1787071921439,"version":"build-2736575974"},"reference-count":39,"publisher":"American Chemical Society (ACS)","issue":"12","license":[{"start":{"date-parts":[[2016,11,14]],"date-time":"2016-11-14T00:00:00Z","timestamp":1479081600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2016,11,14]],"date-time":"2016-11-14T00:00:00Z","timestamp":1479081600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2016,11,14]],"date-time":"2016-11-14T00:00:00Z","timestamp":1479081600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"funder":[{"DOI":"10.13039\/501100004156","name":"Mahidol University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004156","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000268","name":"Biotechnology and Biological Sciences Research Council","doi-asserted-by":"publisher","award":["BB\/E021212\/1"],"award-info":[{"award-number":["BB\/E021212\/1"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2016,12,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Intracellular actin-based motility of the melioidosis pathogen Burkholderia pseudomallei requires the bacterial factor BimA. Located at one pole of the bacterium, BimA recruits and polymerizes cellular actin to promote bacterial motility within and between cells. Here, we describe an affinity approach coupled with mass spectrometry to identify cellular proteins recruited to BimA-expressing bacteria under conditions that promote actin polymerization. We identified a group of cellular proteins that are recruited to the B. pseudomallei surface in a BimA-dependent manner, a subset of which were independently validated with specific antisera including the ubiquitous scaffold protein Ras GTPase-activating-like protein (IQGAP1). IQGAP1 integrates several key cellular signaling pathways including those involved in actin dynamics and has been shown to be involved in the adhesion of attaching and effacing Escherichia coli to infected cells and invasion of host cells by Salmonella enterica serovar Typhimurium. Although a direct interaction between BimA and IQGAP1 could not be detected using either conventional pulldown or yeast two hybrid techniques, confocal microscopy revealed that IQGAP1 is recruited to B. pseudomallei actin tails in infected cells, and siRNA-mediated knockdown highlighted a role for this protein in controlling the length and actin density of B. pseudomallei actin tails.<\/jats:p>","DOI":"10.1021\/acs.jproteome.6b00760","type":"journal-article","created":{"date-parts":[[2016,11,14]],"date-time":"2016-11-14T08:11:13Z","timestamp":1479111073000},"page":"4675-4685","source":"Crossref","is-referenced-by-count":5,"title":["Identification\nof Candidate Host Cell Factors Required\nfor Actin-Based Motility of\n                    <i>Burkholderia pseudomallei<\/i>"],"prefix":"10.1021","volume":"15","author":[{"given":"Niramol","family":"Jitprasutwit","sequence":"first","affiliation":[{"name":"University of Edinburgh , , Easter Bush , , ,","place":["Midlothian, United Kingdom, EH25 9RG"]},{"name":"Mahidol University , ,","place":["Bangkok, Thailand, 73170"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nurhamimah","family":"Zainal-Abidin","sequence":"additional","affiliation":[{"name":"University of Edinburgh , , Easter Bush , , ,","place":["Midlothian, United Kingdom, EH25 9RG"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charles","family":"Vander Broek","sequence":"additional","affiliation":[{"name":"University of Edinburgh , , Easter Bush , , ,","place":["Midlothian, United Kingdom, EH25 9RG"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dominic","family":"Kurian","sequence":"additional","affiliation":[{"name":"University of Edinburgh , , Easter Bush , , ,","place":["Midlothian, United Kingdom, EH25 9RG"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sunee","family":"Korbsrisate","sequence":"additional","affiliation":[{"name":"Mahidol University , ,","place":["Bangkok, Thailand, 73170"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark P.","family":"Stevens","sequence":"additional","affiliation":[{"name":"University of Edinburgh , , Easter Bush , , ,","place":["Midlothian, United Kingdom, EH25 9RG"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9992-2854","authenticated-orcid":true,"given":"Joanne M.","family":"Stevens","sequence":"additional","affiliation":[{"name":"University of Edinburgh , , Easter Bush , , ,","place":["Midlothian, United Kingdom, EH25 9RG"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2016,11,14]]},"reference":[{"issue":"4","key":"2026081810451174300_cit1","doi-asserted-by":"publisher","first-page":"272","DOI":"10.1038\/nrmicro1385","article-title":"Melioidosis:\ninsights into the pathogenicity of Burkholderia pseudomallei","volume":"4","author":"Wiersinga","year":"2006","journal-title":"Nat. Rev. Microbiol."},{"issue":"2","key":"2026081810451174300_cit2","doi-asserted-by":"publisher","first-page":"91","DOI":"10.1038\/nrmicro1320","article-title":"Actin-dependent\nmovement of bacterial\npathogens","volume":"4","author":"Stevens","year":"2006","journal-title":"Nat. Rev. Microbiol."},{"issue":"6","key":"2026081810451174300_cit3","doi-asserted-by":"publisher","first-page":"276","DOI":"10.1016\/j.tcb.2009.03.004","article-title":"New players in actin\npolymerization--WH2-domain-containing\nactin nucleators","volume":"19","author":"Qualmann","year":"2009","journal-title":"Trends Cell Biol."},{"issue":"1","key":"2026081810451174300_cit4","doi-asserted-by":"publisher","first-page":"40","DOI":"10.1111\/j.1365-2958.2004.04528.x","article-title":"Identification\nof a bacterial factor required for actin-based motility of Burkholderia pseudomallei","volume":"56","author":"Stevens","year":"2005","journal-title":"Mol. Microbiol."},{"issue":"7","key":"2026081810451174300_cit5","doi-asserted-by":"publisher","first-page":"2418","DOI":"10.1128\/JCM.00368-08","article-title":"Prevalence and sequence diversity\nof a factor required for actin-based motility in natural populations\nof Burkholderia species","volume":"46","author":"Sitthidet","year":"2008","journal-title":"Journal\nof clinical microbiology"},{"issue":"8","key":"2026081810451174300_cit6","doi-asserted-by":"publisher","first-page":"1901","DOI":"10.1128\/JB.01455-10","article-title":"Identification\nof motifs of Burkholderia pseudomallei BimA required\nfor intracellular motility, actin binding, and actin\npolymerization","volume":"193","author":"Sitthidet","year":"2011","journal-title":"Journal of bacteriology"},{"issue":"4","key":"2026081810451174300_cit7","doi-asserted-by":"publisher","first-page":"e0121271","DOI":"10.1371\/journal.pone.0121271","article-title":"Systematic Mutagenesis\nof Genes Encoding Predicted Autotransported Proteins of Burkholderia\npseudomallei Identifies Factors Mediating Virulence in Mice,\nNet Intracellular Replication and a Novel Protein Conferring Serum\nResistance","volume":"10","author":"Lazar\nAdler","year":"2015","journal-title":"PLoS One"},{"issue":"22","key":"2026081810451174300_cit8","doi-asserted-by":"publisher","first-page":"7857","DOI":"10.1128\/JB.187.22.7857-7862.2005","article-title":"Actin-binding\nproteins from Burkholderia mallei and Burkholderia\nthailandensis can functionally compensate for the actin-based\nmotility defect\nof a Burkholderia pseudomallei bimA mutant","volume":"187","author":"Stevens","year":"2005","journal-title":"Journal of bacteriology"},{"issue":"19","key":"2026081810451174300_cit9a","doi-asserted-by":"publisher","first-page":"5249","DOI":"10.1128\/JB.00608-10","article-title":"Actin-based motility\nof Burkholderia\nthailandensis requires a central acidic domain of BimA that\nrecruits and activates the cellular Arp2\/3 complex","volume":"192","author":"Sitthidet","year":"2010","journal-title":"Journal of bacteriology"},{"issue":"2","key":"2026081810451174300_cit9b","doi-asserted-by":"publisher","first-page":"348","DOI":"10.1016\/j.cell.2015.02.044","article-title":"Virulent Burkholderia species mimic host actin polymerases to drive\nactin-based motility","volume":"161","author":"Benanti","year":"2015","journal-title":"Cell"},{"issue":"3","key":"2026081810451174300_cit10","doi-asserted-by":"publisher","first-page":"408","DOI":"10.1111\/cmi.12376","article-title":"A polar-localized iron-binding\nprotein determines the polar targeting of Burkholderia BimA autotransporter and actin tail formation","volume":"17","author":"Lu","year":"2015","journal-title":"Cell. 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Microbiol."},{"issue":"Pt 19","key":"2026081810451174300_cit13a","doi-asserted-by":"crossref","first-page":"2877","DOI":"10.1242\/jcs.111.19.2877","article-title":"Identification\nof cofilin, coronin, Rac and capZ in actin tails using a Listeria affinity approach","volume":"111","author":"David","year":"1998","journal-title":"Journal of cell science"},{"issue":"2","key":"2026081810451174300_cit13b","doi-asserted-by":"publisher","first-page":"194","DOI":"10.1016\/j.bbrc.2008.07.152","article-title":"The actin\npropulsive machinery: the proteome of Listeria monocytogenes tails","volume":"375","author":"Van Troys","year":"2008","journal-title":"Biochem. Biophys. Res. 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Proteome\nRes."},{"issue":"4","key":"2026081810451174300_cit23","doi-asserted-by":"publisher","first-page":"905","DOI":"10.1074\/mcp.M114.044875","article-title":"Quantitative\nProteomic Analysis of Burkholderia pseudomallei Bsa\nType III Secretion System Effectors Using Hypersecreting Mutants","volume":"14","author":"Vander\nBroek","year":"2015","journal-title":"Mol. Cell. 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A."},{"issue":"46","key":"2026081810451174300_cit26a","doi-asserted-by":"publisher","first-page":"8381","DOI":"10.1016\/j.vaccine.2011.08.058","article-title":"A naturally derived outer-membrane\nvesicle vaccine protects against lethal pulmonary Burkholderia\npseudomallei infection","volume":"29","author":"Nieves","year":"2011","journal-title":"Vaccine"},{"issue":"5","key":"2026081810451174300_cit26b","doi-asserted-by":"publisher","first-page":"747","DOI":"10.1128\/CVI.00119-14","article-title":"A Burkholderia\npseudomallei outer membrane vesicle vaccine provides protection\nagainst lethal sepsis","volume":"21","author":"Nieves","year":"2014","journal-title":"Clinical and vaccine\nimmunology: CVI"},{"issue":"5","key":"2026081810451174300_cit27","doi-asserted-by":"publisher","first-page":"723","DOI":"10.1016\/j.febslet.2011.01.041","article-title":"IQGAP1 in microbial pathogenesis:\nTargeting the actin cytoskeleton","volume":"585","author":"Kim","year":"2011","journal-title":"FEBS Lett."},{"issue":"11","key":"2026081810451174300_cit28","doi-asserted-by":"publisher","first-page":"1057","DOI":"10.1038\/ncb2109","article-title":"Rickettsia Sca2 is a bacterial formin-like mediator of actin-based\nmotility","volume":"12","author":"Haglund","year":"2010","journal-title":"Nat. Cell Biol."},{"issue":"6613","key":"2026081810451174300_cit29a","doi-asserted-by":"publisher","first-page":"265","DOI":"10.1038\/385265a0","article-title":"Actin polymerization\nis induced by Arp2\/3 protein complex at the surface of Listeria\nmonocytogenes","volume":"385","author":"Welch","year":"1997","journal-title":"Nature"},{"issue":"5373","key":"2026081810451174300_cit29b","doi-asserted-by":"publisher","first-page":"105","DOI":"10.1126\/science.281.5373.105","article-title":"Interaction\nof human Arp2\/3 complex and the Listeria monocytogenes ActA protein in actin filament nucleation","volume":"281","author":"Welch","year":"1998","journal-title":"Science"},{"issue":"6753","key":"2026081810451174300_cit30","doi-asserted-by":"publisher","first-page":"613","DOI":"10.1038\/44183","article-title":"Reconstitution of\nactin-based motility of Listeria and Shigella using pure proteins","volume":"401","author":"Loisel","year":"1999","journal-title":"Nature"},{"issue":"8","key":"2026081810451174300_cit31","doi-asserted-by":"publisher","first-page":"4706","DOI":"10.1128\/IAI.68.8.4706-4713.2000","article-title":"Ultrastructure of Rickettsia rickettsii actin tails and localization of cytoskeletal\nproteins","volume":"68","author":"Van\nKirk","year":"2000","journal-title":"Infection and immunity"},{"issue":"4","key":"2026081810451174300_cit32a","doi-asserted-by":"publisher","first-page":"231","DOI":"10.1002\/cm.20013","article-title":"Actin filament binding by a monomeric\nIQGAP1 fragment with a single calponin homology domain","volume":"58","author":"Mateer","year":"2004","journal-title":"Cell Motil. Cytoskeleton"},{"issue":"39","key":"2026081810451174300_cit32b","doi-asserted-by":"publisher","first-page":"24443","DOI":"10.1074\/jbc.272.39.24443","article-title":"Identification of\nan actin cytoskeletal complex that includes IQGAP and the Cdc42 GTPase","volume":"272","author":"Erickson","year":"1997","journal-title":"J. Biol. Chem."}],"container-title":["Journal of Proteome Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acs.jproteome.6b00760","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/jprobs\/article-pdf\/15\/12\/4675\/15517129\/pr6b00760.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/jprobs\/article-pdf\/15\/12\/4675\/15517129\/pr6b00760.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T16:06:12Z","timestamp":1787069172000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/jprobs\/article\/15\/12\/4675\/1464985\/Identification-of-Candidate-Host-Cell-Factors"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,11,14]]},"references-count":39,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2016,11,14]]},"published-print":{"date-parts":[[2016,12,2]]}},"URL":"https:\/\/doi.org\/10.1021\/acs.jproteome.6b00760","relation":{},"ISSN":["1535-3893","1535-3907"],"issn-type":[{"value":"1535-3893","type":"print"},{"value":"1535-3907","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,11,14]]}}}