{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T15:22:58Z","timestamp":1788535378030,"version":"build-2803163510"},"reference-count":34,"publisher":"American Chemical Society (ACS)","issue":"9","license":[{"start":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T00:00:00Z","timestamp":1786320000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,9,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Food residues that bypass human digestion are further digested by gut microbes, leading to the production of diverse metabolites, including lipids. To investigate how lipids in our intestine are affected during this transition, we used a colon simulator with four distinct vessels (V1\u2013V4) that mimic the proximal to distal part of the human colon. In total, 44 samples were collected from the colon simulator vessels (V1\u2013V4). We observed dynamic shifts in a diverse array of microbially linked lipid molecules in the simulated intestinal chyme, including bile acids and N-acyl amides with short- and odd-chain lipids. Histamine-linked N-acyl lipids (histamine-C5) increased from the proximal to the distal colon vessels (pH 5.5\u20137.0), whereas putrescine-linked ones (putrescine-C19:2), initially abundant in the media, decreased across the colon vessels. We uncovered dynamic associations between in vitro-derived short-chain N-acyl lipids (C4:0, C5, C6, and C7 conjugates) and major lipid species such as cholesterol esters, phosphatidylethanolamines, ceramides, and sphingomyelins. To determine the broader relevance of these findings, we applied a reverse metabolomics approach and examined N-acyl lipid profiles in human small intestine and fecal samples from public data sets. Our results validate the colon simulator as a dynamic model for studying microbially transformed metabolites and suggest its potential utility as a platform for discovering novel microbial metabolites with relevance to human and animal health.<\/jats:p>","DOI":"10.1021\/acs.jproteome.6c00050","type":"journal-article","created":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T22:02:18Z","timestamp":1786399338000},"page":"4577-4588","source":"Crossref","is-referenced-by-count":0,"title":["Emerging Microbial\nLipid Shifts in a Multistage Simulated\nGut Inoculated with Human Fecal Microbiota"],"prefix":"10.1021","volume":"25","author":[{"given":"Ifrat","family":"Tamanna","sequence":"first","affiliation":[{"name":"University of Turku and Turku University Hospital , , ,","place":["Turku, Finland, 20520"]},{"name":"University of Turku and \u00c5bo Akademi University , , ,","place":["Turku, Finland, 20520"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katja","family":"Salonen","sequence":"additional","affiliation":[{"name":"University of Turku and \u00c5bo Akademi University , , ,","place":["Turku, Finland, 20520"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helena","family":"Mannochio-Russo","sequence":"additional","affiliation":[{"name":"University of California San Diego , , , ,","place":["La Jolla, California, United States, 92093-065"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matilda","family":"Kr\u00e5kstro\u0308m","sequence":"additional","affiliation":[{"name":"University of Turku and \u00c5bo Akademi University , , ,","place":["Turku, Finland, 20520"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5478-2448","authenticated-orcid":true,"given":"Paulo Wender P.","family":"Gomes","sequence":"additional","affiliation":[{"name":"Federal University of Para\u0301 , , , ,","place":["Bele\u0301m, PA, Brazil, 66075-110"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vincent","family":"Charron-Lamoureux","sequence":"additional","affiliation":[{"name":"University of California San Diego , , , ,","place":["La Jolla, California, United States, 92093-065"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5311-6443","authenticated-orcid":true,"given":"Ipsita","family":"Mohanty","sequence":"additional","affiliation":[{"name":"University of California San Diego , , , ,","place":["La Jolla, California, United States, 92093-065"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sofia D.","family":"Forssten","sequence":"additional","affiliation":[{"name":"IFF Health Sciences , ,","place":["Kantvik, Finland, 02460"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arthur C.","family":"Ouwehand","sequence":"additional","affiliation":[{"name":"IFF Health Sciences , ,","place":["Kantvik, Finland, 02460"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1389-8302","authenticated-orcid":true,"given":"Tuulia","family":"Hyo\u0308tyla\u0308inen","sequence":"additional","affiliation":[{"name":"O\u0308rebro University , , ,","place":["O\u0308rebro, Sweden, 70281"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3178-8449","authenticated-orcid":true,"given":"Alex M.","family":"Dickens","sequence":"additional","affiliation":[{"name":"University of Turku and \u00c5bo Akademi University , , ,","place":["Turku, Finland, 20520"]},{"name":"University of Turku , , ,","place":["Turku, Finland, 20520"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3003-1030","authenticated-orcid":true,"given":"Pieter C.","family":"Dorrestein","sequence":"additional","affiliation":[{"name":"University of California San Diego , , , 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Wellbeing Services County of Southwest Finland , , ,","place":["Turku, Finland, 20520"]},{"name":"Turku University Hospital , , ,","place":["Turku, Finland, 20520"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,8,10]]},"reference":[{"key":"2026090409140458300_cit1","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1016\/j.ymeth.2018.04.029","article-title":"Gut metabolome\nmeets microbiome: A methodological perspective to understand the relationship\nbetween host and microbe","volume":"149","author":"Lamichhane","year":"2018","journal-title":"Methods"},{"issue":"6","key":"2026090409140458300_cit2","doi-asserted-by":"publisher","first-page":"341","DOI":"10.1038\/nri.2016.42","article-title":"Gut microbiota,\nmetabolites and host immunity","volume":"16","author":"Rooks","year":"2016","journal-title":"Nat. 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