{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T15:17:04Z","timestamp":1788535024072,"version":"build-2803163510"},"reference-count":42,"publisher":"American Chemical Society (ACS)","issue":"9","license":[{"start":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T00:00:00Z","timestamp":1785715200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005366","name":"Universitetet i Oslo","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005366","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,9,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Smart sampling is a concept that combines a traditional microsampling, such as dried blood spot (DBS), with sample preparation initiated at the moment of sampling. In this paper, plasma separation and antibody-based affinity cleanup are integrated into a single 3D-printed sampling device. Our goal was to evaluate and validate the analytical performance of this device for LC-MS-based determination of hCG. The device effectively eliminated red blood cells, significantly reducing hemoglobin interference by more than 93%. Plasma extraction of &amp;gt;80% was achieved from only 20 \u03bcL of whole blood. The partial validation in line with the European Medicines Agency\u2019s ICH guidelines showed that the analytical performance of the sampling device was not compromised. Linear correlation coefficients \u22650.991 were achieved (range: 0.5\u201375 ng\/mL). High between-run accuracy for all QC levels were achieved, ranging from 90 to 105% with a corresponding precision of \u226411%. In conclusion, the 3D-printed device offers a robust solution to traditional microsampling limitations, potentially and in the future enabling point-of-care diagnostics with the versatility and ease of established DBS methods. The successful integration of plasma separation and smart sampling enhances analytical sensitivity and specificity, representing a promising tool for advancements in clinical bioanalysis.<\/jats:p>","DOI":"10.1021\/acs.jproteome.6c00092","type":"journal-article","created":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T13:02:14Z","timestamp":1785934934000},"page":"4665-4675","source":"Crossref","is-referenced-by-count":0,"title":["Dual Functionality\nin Whole Blood Microsampling: Combining\nPlasma Separation and Immunoaffinity Cleanup for LC\u2013MS Based\nTargeted Protein Analysis"],"prefix":"10.1021","volume":"25","author":[{"given":"Ago","family":"Mrsa","sequence":"first","affiliation":[{"name":"University of Oslo , , P.O. Box 1068 Blindern, ,","place":["Oslo, Norway, N-0316"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6828-6381","authenticated-orcid":true,"given":"Bjarke","family":"Str\u00f8m Larsen","sequence":"additional","affiliation":[{"name":"University of Oslo , , P.O. Box 1068 Blindern, ,","place":["Oslo, Norway, N-0316"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2545-3474","authenticated-orcid":true,"given":"Trine","family":"Gr\u00f8nhaug Halvorsen","sequence":"additional","affiliation":[{"name":"University of Oslo , , P.O. Box 1068 Blindern, ,","place":["Oslo, Norway, N-0316"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5165-2701","authenticated-orcid":true,"given":"Le\u0301on","family":"Reubsaet","sequence":"additional","affiliation":[{"name":"University of Oslo , , P.O. 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