{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T19:44:51Z","timestamp":1792784691025,"version":"4.5.0"},"reference-count":32,"publisher":"American Chemical Society (ACS)","issue":"10","license":[{"start":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T00:00:00Z","timestamp":1788393600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T00:00:00Z","timestamp":1788393600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T00:00:00Z","timestamp":1788393600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"funder":[{"DOI":"10.13039\/501100008235","name":"Peking Union Medical College Hospital","doi-asserted-by":"publisher","award":["2025-PUMCH-035"],"award-info":[{"award-number":["2025-PUMCH-035"]}],"id":[{"id":"10.13039\/501100008235","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008235","name":"Peking Union Medical College Hospital","doi-asserted-by":"publisher","award":["UBJ06514"],"award-info":[{"award-number":["UBJ06514"]}],"id":[{"id":"10.13039\/501100008235","id-type":"DOI","asserted-by":"publisher"}]},{"name":"National Natural Science Foundation of China (NSFC)","award":["82170397"],"award-info":[{"award-number":["82170397"]}],"id":[{"id":"https:\/\/ror.org\/01h0zpd94","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,10,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Reliable noninvasive discrimination of acute myocarditis (AM) from acute myocardial infarction (AMI) in patients presenting with acute chest pain remains an unmet clinical need. We aimed to identify urinary proteomic markers using mass spectrometry and LASSO regression. Patients with suspected AM or AMI were prospectively enrolled. Urinary proteomes were profiled by data-independent acquisition mass spectrometry. The discovery cohort was stratified by age and sex and randomly divided into training and internal validation sets at a 3:1 ratio. Differentially expressed proteins were screened, and a diagnostic model was constructed using LASSO with 1000 bootstrap resamplings in the training set. The model was validated internally and further verified in an independent cohort using ELISA. A total of 42 AM and 80 AMI patients were included in the discovery cohort. Differential expression analysis in the training set identified 880 and 1099 differentially expressed proteins in AM and AMI, respectively, compared with controls. A three-protein panel comprising TNFRSF1A, F9, and PRDX3 achieved an AUC of 0.954 in the training set and exceeded the clinical models (all DeLong\u2019s P &amp;lt; 0.05). Addition of the protein panel to either clinical model further improved diagnostic performance (both P &amp;lt; 0.001). Similar performance was confirmed in the validation cohorts. A urinary proteomic panel comprising TNFRSF1A, F9, and PRDX3 may provide a noninvasive approach for differentiating AM from AMI.<\/jats:p>","DOI":"10.1021\/acs.jproteome.6c00106","type":"journal-article","created":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T21:38:56Z","timestamp":1788471536000},"page":"5189-5199","source":"Crossref","is-referenced-by-count":0,"title":["Urinary Proteomic\nPanel Distinguishes Acute Myocarditis\nfrom Acute Myocardial Infarction"],"prefix":"10.1021","volume":"25","author":[{"given":"Yufei","family":"Wang","sequence":"first","affiliation":[{"name":"Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College , , ,","place":["Beijing, China, 100730"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuhan","family":"Qin","sequence":"additional","affiliation":[{"name":"Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College , , ,","place":["Beijing, China, 100730"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Yang","sequence":"additional","affiliation":[{"name":"Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College , , ,","place":["Beijing, China, 100730"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4142-3953","authenticated-orcid":true,"given":"Qinghai","family":"Wang","sequence":"additional","affiliation":[{"name":"The Second Qilu Hospital of Shandong University , , ,","place":["Jinan, China, 250033"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-8485-6621","authenticated-orcid":true,"given":"Aiwei","family":"Wang","sequence":"additional","affiliation":[{"name":"Peking Union Medical College , , ,","place":["Beijing, China, 100005"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Zhang","sequence":"additional","affiliation":[{"name":"Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College , , ,","place":["Beijing, China, 100730"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anhu","family":"Wu","sequence":"additional","affiliation":[{"name":"Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College , , ,","place":["Beijing, China, 100730"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaohan","family":"Qin","sequence":"additional","affiliation":[{"name":"Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College , , ,","place":["Beijing, China, 100730"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiaqi","family":"Yu","sequence":"additional","affiliation":[{"name":"Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College , , ,","place":["Beijing, China, 100730"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenyu","family":"Liu","sequence":"additional","affiliation":[{"name":"Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College , , ,","place":["Beijing, China, 100730"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8583-5992","authenticated-orcid":true,"given":"Xiaoxiao","family":"Guo","sequence":"additional","affiliation":[{"name":"Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College , , ,","place":["Beijing, China, 100730"]},{"name":"Chinese Academy of Medical Sciences and Peking Union Medical College , , ,","place":["Beijing, China, 100730"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,9,3]]},"reference":[{"issue":"16","key":"2026100208412768800_cit1","doi-asserted-by":"publisher","first-page":"1488","DOI":"10.1056\/NEJMra2114478","article-title":"Myocarditis","volume":"387","author":"Basso","year":"2022","journal-title":"N. Engl. J. Med."},{"issue":"13","key":"2026100208412768800_cit2","doi-asserted-by":"publisher","first-page":"1098","DOI":"10.1001\/jama.2023.3371","article-title":"Diagnosis and Treatment\nof Acute Myocarditis: A Review","volume":"329","author":"Ammirati","year":"2023","journal-title":"JAMA"},{"issue":"7","key":"2026100208412768800_cit3","doi-asserted-by":"publisher","first-page":"662","DOI":"10.1001\/jama.2022.0358","article-title":"Diagnosis and Treatment of Acute\nCoronary Syndromes: A Review","volume":"327","author":"Bhatt","year":"2022","journal-title":"JAMA"},{"issue":"3","key":"2026100208412768800_cit4","doi-asserted-by":"publisher","first-page":"143","DOI":"10.1093\/eurheartj\/ehw149","article-title":"ESC Working Group Position Paper\non Myocardial Infarction with Non-Obstructive Coronary Arteries","volume":"38","author":"Agewall","year":"2017","journal-title":"Eur. Heart J."},{"issue":"3","key":"2026100208412768800_cit5","doi-asserted-by":"publisher","first-page":"496","DOI":"10.1161\/CIRCRESAHA.115.306573","article-title":"Myocarditis","volume":"118","author":"Fung","year":"2016","journal-title":"Circ. Res."},{"issue":"11","key":"2026100208412768800_cit6","doi-asserted-by":"publisher","first-page":"1875","DOI":"10.1002\/ejhf.2195","article-title":"Urinary Peptides\nin Heart Failure: A Link to Molecular Pathophysiology","volume":"23","author":"He","year":"2021","journal-title":"Eur. J. Heart Failure"},{"issue":"10","key":"2026100208412768800_cit7","doi-asserted-by":"publisher","first-page":"1850","DOI":"10.1074\/mcp.R800001-MCP200","article-title":"Urine in Clinical Proteomics","volume":"7","author":"Decramer","year":"2008","journal-title":"Mol. Cell Proteomics"},{"issue":"7","key":"2026100208412768800_cit8","doi-asserted-by":"publisher","first-page":"822","DOI":"10.1002\/ejhf.544","article-title":"Urine Proteome\nAnalysis in Heart Failure with Reduced Ejection Fraction Complicated\nby Chronic Kidney Disease: Feasibility, and Clinical and Pathogenetic\nCorrelates","volume":"18","author":"Farmakis","year":"2016","journal-title":"Eur. J. Heart Failure"},{"issue":"4","key":"2026100208412768800_cit9","doi-asserted-by":"publisher","first-page":"361","DOI":"10.1503\/cmaj.061590","article-title":"Urine Proteomics: The\nPresent and Future of Measuring\nUrinary Protein Components in Disease","volume":"177","author":"Barratt","year":"2007","journal-title":"CMAJ"},{"issue":"13","key":"2026100208412768800_cit10","doi-asserted-by":"publisher","first-page":"e771","DOI":"10.1161\/CIR.0000000000001309","article-title":"2025 ACC\/AHA\/ACEP\/NAEMSP\/SCAI Guideline\nfor the Management of Patients With Acute Coronary Syndromes: A Report\nof the American College of Cardiology\/American Heart Association Joint\nCommittee on Clinical Practice Guidelines","volume":"151","author":"Rao","year":"2025","journal-title":"Circulation"},{"issue":"3","key":"2026100208412768800_cit11","doi-asserted-by":"publisher","first-page":"237","DOI":"10.1093\/eurheartj\/ehy462","article-title":"Fourth\nUniversal Definition of Myocardial Infarction (2018)","volume":"40","author":"Thygesen","year":"2019","journal-title":"Eur. Heart J."},{"issue":"24","key":"2026100208412768800_cit12","doi-asserted-by":"publisher","first-page":"3158","DOI":"10.1016\/j.jacc.2018.09.072","article-title":"Cardiovascular\nMagnetic Resonance in Nonischemic Myocardial Inflammation: Expert\nRecommendations","volume":"72","author":"Ferreira","year":"2018","journal-title":"J. Am. Coll Cardiol"},{"issue":"33","key":"2026100208412768800_cit13","doi-asserted-by":"publisher","first-page":"2636","DOI":"10.1093\/eurheartj\/eht210","article-title":"Current State of Knowledge on Aetiology, Diagnosis,\nManagement, and Therapy of Myocarditis: A Position Statement of the\nEuropean Society of Cardiology Working Group on Myocardial and Pericardial\nDiseases","volume":"34","author":"Caforio","year":"2013","journal-title":"Eur. Heart J."},{"issue":"21","key":"2026100208412768800_cit14","doi-asserted-by":"publisher","first-page":"2014","DOI":"10.1056\/NEJMoa2003608","article-title":"A Novel Circulating\nMicroRNA for the Detection of Acute Myocarditis","volume":"384","author":"Blanco-Domi\u0301nguez","year":"2021","journal-title":"N. Engl. J. 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Diabetol."},{"issue":"2","key":"2026100208412768800_cit21","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0055477","article-title":"Soluble TNF Receptors\nAre Associated with Infarct Size and Ventricular Dysfunction in ST-Elevation\nMyocardial Infarction","volume":"8","author":"Nilsson","year":"2013","journal-title":"PLoS One"},{"issue":"2","key":"2026100208412768800_cit22","doi-asserted-by":"publisher","first-page":"206","DOI":"10.1161\/CIRCULATIONAHA.118.036065","article-title":"Dysfunctional\nand Proinflammatory\nRegulatory T-Lymphocytes Are Essential for Adverse Cardiac Remodeling\nin Ischemic Cardiomyopathy","volume":"139","author":"Bansal","year":"2019","journal-title":"Circulation"},{"issue":"5","key":"2026100208412768800_cit23","doi-asserted-by":"publisher","first-page":"1871","DOI":"10.1182\/blood-2002-05-1581","article-title":"Myocardial\nFibrosis in Mice with Overexpression of Human Blood Coagulation Factor\nIX","volume":"101","author":"Ameri","year":"2003","journal-title":"Blood"},{"issue":"11","key":"2026100208412768800_cit24","doi-asserted-by":"publisher","first-page":"2489","DOI":"10.1161\/01.atv.20.11.2489","article-title":"Activation\nof Clotting Factors XI and IX in Patients with Acute Myocardial Infarction","volume":"20","author":"Minnema","year":"2000","journal-title":"Arterioscler., Thromb., Vasc. Biol."},{"issue":"13","key":"2026100208412768800_cit25","doi-asserted-by":"publisher","first-page":"4045","DOI":"10.1182\/blood-2005-12-023697","article-title":"Levels of Intrinsic Coagulation Factors\nand the Risk of Myocardial Infarction among Men: Opposite and Synergistic\nEffects of Factors XI and XII","volume":"108","author":"Doggen","year":"2006","journal-title":"Blood"},{"issue":"14","key":"2026100208412768800_cit26","doi-asserted-by":"publisher","first-page":"1455","DOI":"10.1001\/jama.1981.03310390055022","article-title":"Myocardial Infarction after Factor IX Therapy","volume":"245","author":"Fuerth","year":"1981","journal-title":"JAMA"},{"issue":"19","key":"2026100208412768800_cit27","doi-asserted-by":"publisher","first-page":"2412","DOI":"10.1093\/eurheartj\/ehr179","article-title":"Pegnivacogin\nResults in near Complete FIX Inhibition in Acute Coronary Syndrome\nPatients: RADAR Pharmacokinetic and Pharmacodynamic Substudy","volume":"32","author":"Povsic","year":"2011","journal-title":"Eur. Heart J."},{"key":"2026100208412768800_cit28","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2022.102275","article-title":"Peroxiredoxin\n3 Deficiency Induces Cardiac Hypertrophy and Dysfunction by Impaired\nMitochondrial Quality Control","volume":"51","author":"Sonn","year":"2022","journal-title":"Redox Biol."},{"issue":"6","key":"2026100208412768800_cit29","doi-asserted-by":"publisher","first-page":"592","DOI":"10.1080\/10409238.2020.1828258","article-title":"Riding\nthe Tiger\u2013Physiological and Pathological Effects of Superoxide\nand Hydrogen Peroxide Generated in the Mitochondrial Matrix","volume":"55","author":"Brand","year":"2020","journal-title":"Crit. Rev. Biochem. Mol. Biol."},{"issue":"12","key":"2026100208412768800_cit30","doi-asserted-by":"publisher","DOI":"10.3390\/antiox14121504","article-title":"Extracellular\nVesicle-Mediated Delivery of Antioxidant Enzymes: Emerging Insights\nand Translational Opportunities","volume":"14","author":"Wang","year":"2025","journal-title":"Antioxidants"},{"key":"2026100208412768800_cit31","doi-asserted-by":"publisher","DOI":"10.3389\/fbioe.2021.752019","article-title":"Extracellular\nVesicles as Natural Delivery Carriers Regulate Oxidative Stress Under\nPathological Conditions","volume":"9","author":"Qi","year":"2021","journal-title":"Front. Bioeng. Biotechnol."},{"issue":"7","key":"2026100208412768800_cit32","doi-asserted-by":"publisher","DOI":"10.3390\/cells10071763","article-title":"Extracellular Vesicles\nunder Oxidative Stress Conditions: Biological Properties and Physiological\nRoles","volume":"10","author":"Chiaradia","year":"2021","journal-title":"Cells"}],"container-title":["Journal of Proteome Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acs.jproteome.6c00106","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/jprobs\/article-pdf\/25\/10\/5189\/68301524\/acs.jproteome.6c00106.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/jprobs\/article-pdf\/25\/10\/5189\/68301524\/acs.jproteome.6c00106.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T12:51:47Z","timestamp":1790945507000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/jprobs\/article\/25\/10\/5189\/5414837\/Urinary-Proteomic-Panel-Distinguishes-Acute"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,3]]},"references-count":32,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2026,9,3]]},"published-print":{"date-parts":[[2026,10,2]]}},"URL":"https:\/\/doi.org\/10.1021\/acs.jproteome.6c00106","relation":{},"ISSN":["1535-3893","1535-3907"],"issn-type":[{"value":"1535-3893","type":"print"},{"value":"1535-3907","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,3]]}}}