{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T17:17:22Z","timestamp":1786123042750,"version":"build-2736575974"},"reference-count":30,"publisher":"American Chemical Society (ACS)","issue":"8","license":[{"start":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T00:00:00Z","timestamp":1783987200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004063","name":"Knut och Alice Wallenbergs Stiftelse","doi-asserted-by":"publisher","award":["KAW 2020.0239"],"award-info":[{"award-number":["KAW 2020.0239"]}],"id":[{"id":"10.13039\/501100004063","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100009252","name":"Science for Life Laboratory","doi-asserted-by":"publisher","award":["NA"],"award-info":[{"award-number":["NA"]}],"id":[{"id":"10.13039\/501100009252","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,8,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Targeted proteomics offers high precision, reproducibility, and multiplexing capabilities for quantifying proteins in complex biological samples, making the technology into a powerful tool for biomarker discovery and clinical diagnostics. When combined with at-home microsampling, this approach has the potential to transform population-level screening and longitudinal studies and can pave the way for decentralized healthcare. One of the main advantages of this combined approach is that samples can be collected offsite and then transported and stored as dried blood spots (DBSs). Currently, innovative volumetric microfluidic devices have also enabled DBS microsampling of consistently precise volumes. However, the reliability of the molecular data obtained with this mode of sampling still needs to be investigated and validated. In this study, 72 deidentified blood samples from two individuals were collected using DBS microsampling device CapitainerB and analyzed using targeted mass spectrometry and quantitative recombinant protein standards (qRePSs). The DBSs were subjected to three simulated and realistic shipping environmental conditions and stored in two different types of containers to assess sample stability. The results suggest that shipping conditions and storage at high temperatures often observed during summer have an impact on protein identification and quantification results, highlighting the need for further research to ensure biomarker reliability for dispersed microsampling.<\/jats:p>","DOI":"10.1021\/acs.jproteome.6c00117","type":"journal-article","created":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T15:04:25Z","timestamp":1784041465000},"page":"3817-3825","source":"Crossref","is-referenced-by-count":0,"title":["Shipping Conditions\nMay Impact the Reliability of\nAt-Home Microsampling Devices"],"prefix":"10.1021","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-5973-2814","authenticated-orcid":true,"given":"Andrea","family":"Villanueva Raisman","sequence":"first","affiliation":[{"name":"KTH Royal Institute of Technology , , ,","place":["Solna, Sweden, 171 65"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jakob","family":"Naess","sequence":"additional","affiliation":[{"name":"KTH Royal Institute of Technology , , ,","place":["Solna, Sweden, 171 65"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Kotol","sequence":"additional","affiliation":[{"name":"ProteomEdge AB , ,","place":["Stockholm, Sweden, 106 91"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0017-7987","authenticated-orcid":true,"given":"Fredrik","family":"Edfors","sequence":"additional","affiliation":[{"name":"KTH Royal Institute of Technology , , ,","place":["Solna, Sweden, 171 65"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,7,14]]},"reference":[{"issue":"6220","key":"2026080709081851700_cit1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1260419","article-title":"Tissue-based\nmap of the human proteome","volume":"347","author":"Uhle\u0301n","year":"2015","journal-title":"Science"},{"issue":"5","key":"2026080709081851700_cit2","first-page":"582","article-title":"Complications\noccurring from diagnostic venipuncture","volume":"34","author":"Galena","year":"1992","journal-title":"Journal\nof Family Practice"},{"issue":"5","key":"2026080709081851700_cit3","doi-asserted-by":"publisher","first-page":"339","DOI":"10.1097\/01.moh.0000142105.21058.96","article-title":"Blood donor\ncomplications after whole-blood donation","volume":"11","author":"Newman","year":"2004","journal-title":"Current\nOpinion in Hematology"},{"issue":"1","key":"2026080709081851700_cit4","doi-asserted-by":"publisher","first-page":"109","DOI":"10.3109\/J426v01n01_17","article-title":"Venipuncture-induced\nnerve injury: a review","volume":"1","author":"Horowitz","year":"2005","journal-title":"Journal of Neuropathic\nPain & Symptom Palliation"},{"issue":"3","key":"2026080709081851700_cit5","doi-asserted-by":"publisher","first-page":"338","DOI":"10.1542\/peds.32.3.338","article-title":"A simple phenylalanine\nmethod for detecting phenylketonuria in large\npopulations of newborn infants","volume":"32","author":"Guthrie","year":"1963","journal-title":"Pediatrics"},{"issue":"1","key":"2026080709081851700_cit6","doi-asserted-by":"publisher","first-page":"55","DOI":"10.1038\/s43856-024-00480-4","article-title":"Proteome profiling of home-sampled\ndried blood spots reveals proteins of SARS-CoV-2 infections","volume":"4","author":"Fredolini","year":"2024","journal-title":"Communications Medicine"},{"issue":"12","key":"2026080709081851700_cit7","doi-asserted-by":"publisher","first-page":"5279","DOI":"10.1021\/acs.jproteome.4c00586","article-title":"The Circulating\nProteome\u2013Technological Developments,\nCurrent Challenges, and Future Trends","volume":"23","author":"Geyer","year":"2024","journal-title":"J. Proteome\nRes."},{"key":"2026080709081851700_cit8","doi-asserted-by":"publisher","first-page":"25","DOI":"10.1016\/j.clinms.2017.08.004","article-title":"Application of volumetric absorptive\nmicrosampling for robust, high-throughput mass spectrometric quantification\nof circulating protein biomarkers","volume":"4","author":"van den Broek","year":"2017","journal-title":"Clinical\nMass Spectrometry"},{"issue":"12","key":"2026080709081851700_cit9","doi-asserted-by":"publisher","first-page":"2433","DOI":"10.1074\/mcp.RA119.001765","article-title":"Absolute quantification\nof apolipoproteins following treatment with omega-3 carboxylic acids\nand fenofibrate using a high precision stable isotope-labeled recombinant\nprotein fragments based SRM assay","volume":"18","author":"Hober","year":"2019","journal-title":"Molecular\n& Cellular Proteomics"},{"issue":"3","key":"2026080709081851700_cit10","doi-asserted-by":"publisher","first-page":"473","DOI":"10.2144\/btn-2021-0047","article-title":"Targeted proteomics\nanalysis of plasma proteins using\nrecombinant protein standards for addition only workflows","volume":"71","author":"Kotol","year":"2021","journal-title":"Biotechniques"},{"issue":"19","key":"2026080709081851700_cit11","doi-asserted-by":"publisher","first-page":"4764","DOI":"10.3390\/cancers15194764","article-title":"Absolute quantification of pan-cancer\nplasma proteomes reveals unique signature in multiple myeloma","volume":"15","author":"Kotol","year":"2023","journal-title":"Cancers"},{"key":"2026080709081851700_cit12","volume-title":"Temperature Test for\nTransport Packaging","author":"ISTA.","year":"2018"},{"issue":"3","key":"2026080709081851700_cit13","doi-asserted-by":"publisher","first-page":"185","DOI":"10.1016\/j.cels.2016.02.015","article-title":"Plasma proteome profiling to assess human health and disease","volume":"2","author":"Geyer","year":"2016","journal-title":"Cell Systems"},{"issue":"8","key":"2026080709081851700_cit14","doi-asserted-by":"publisher","first-page":"1896","DOI":"10.1038\/nprot.2007.261","article-title":"Protocol for micro-purification,\nenrichment, pre-fractionation and storage of peptides for proteomics\nusing StageTips","volume":"2","author":"Rappsilber","year":"2007","journal-title":"Nat. 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