{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T17:16:07Z","timestamp":1786122967295,"version":"build-2736575974"},"reference-count":72,"publisher":"American Chemical Society (ACS)","issue":"8","license":[{"start":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T00:00:00Z","timestamp":1782777600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001322","name":"South African Medical Research Council","doi-asserted-by":"publisher","award":["NA"],"award-info":[{"award-number":["NA"]}],"id":[{"id":"10.13039\/501100001322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001336","name":"University of the Witwatersrand, Johannesburg","doi-asserted-by":"publisher","award":["NA"],"award-info":[{"award-number":["NA"]}],"id":[{"id":"10.13039\/501100001336","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006312","name":"South African Agency for Science and Technology Advancement","doi-asserted-by":"publisher","award":["CSRP240407212642"],"award-info":[{"award-number":["CSRP240407212642"]}],"id":[{"id":"10.13039\/501100006312","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,8,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Pancreatic ductal adenocarcinoma (PDAC) is a highly aggressive cancer and was ranked among the top seven leading causes of cancer-related deaths in South Africa in 2020. Highlighting the urgent need for ongoing research to identify reliable biomarkers to improve clinical outcomes. This study compared the plasma proteomes of patients with PDAC, those with benign biliary pathologies (BBP), and healthy controls (HC). We used Mag-Net, a magnetic-bead-based method that enriches membrane-bound vesicles. Comparative analyses identified distinct and overlapping dysregulated proteins between PDAC, BBP, and HC. PDAC showed enrichment for epithelial-mesenchymal transition, complement activation, hypoxia, glycolysis, and extracellular matrix remodeling pathways, consistent with aggressive tumor biology. Proteins including SPP1, THBS2, PTX3, FBLN2, SDC1, CTSS, and VCAN were significantly increased in PDAC, with LRG1 showing the strongest association with disease severity. Proteins, namely GPNMB, H4C1, SAA1, and CCDC47, demonstrated progressive upregulation across disease comparisons, suggesting potential relevance to disease progression. These findings demonstrate that plasma proteomics provides discriminatory molecular insights and supports the development of population-relevant biomarker panels. While several candidate proteins show promise for inclusion in multianalyte panels, further validation in larger cohorts is necessary to establish their diagnostic and translational utility for early detection, risk stratification, and improved differential diagnosis of PDAC.<\/jats:p>","DOI":"10.1021\/acs.jproteome.6c00172","type":"journal-article","created":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T20:57:12Z","timestamp":1782853032000},"page":"4120-4131","source":"Crossref","is-referenced-by-count":0,"title":["Proteomic Profiling\nof Extracellular Vesicle-Enriched\nPlasma Using Mag-Net for Biomarker Discovery in Pancreatic Ductal\nAdenocarcinoma"],"prefix":"10.1021","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6745-6155","authenticated-orcid":true,"given":"Sindisiwe","family":"Buthelezi","sequence":"first","affiliation":[{"name":"Council for Scientific and Industrial Research , , ,","place":["Pretoria, South Africa, 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5230"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeanet","family":"Mazibuko","sequence":"additional","affiliation":[{"name":"University of Witwatersrand , , ,","place":["Johannesburg, South Africa, 2193"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dupe","family":"Ojo","sequence":"additional","affiliation":[{"name":"ReSyn Biosciences , ,","place":["Gauteng, South Africa, 1610"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Geoffrey","family":"Candy","sequence":"additional","affiliation":[{"name":"University of Witwatersrand , , ,","place":["Johannesburg, South Africa, 2193"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John","family":"Devar","sequence":"additional","affiliation":[{"name":"University of Witwatersrand , , ,","place":["Johannesburg, South Africa, 2193"]},{"name":"Chris Hani-Baragwanath Academic Hospital , , , , ,","place":["Soweto, Johannesburg, South Africa, 1860"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefano","family":"Cacciatore","sequence":"additional","affiliation":[{"name":"University of Cape Town , , ,","place":["Cape Town, South Africa, 7701"]},{"name":"International Centre for Genetic Engineering and Biotechnology , , Observatory, ,","place":["Cape Town, South Africa, 7925"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jones","family":"Omoshoro-Jones","sequence":"additional","affiliation":[{"name":"University of Witwatersrand , , ,","place":["Johannesburg, South Africa, 2193"]},{"name":"Chris Hani-Baragwanath Academic Hospital , , , , ,","place":["Soweto, Johannesburg, South Africa, 1860"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ekene Emmanuel","family":"Nweke","sequence":"additional","affiliation":[{"name":"University of Witwatersrand , , ,","place":["Johannesburg, South Africa, 2193"]},{"name":"University of South Africa , , , ,","place":["Roodepoort, Florida, South Africa, 1709"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,6,30]]},"reference":[{"issue":"9","key":"2026080709082615400_cit1","first-page":"4294","article-title":"A Prognostic Score\nBased on Long-Term Survivor Unique Transcriptomic Signatures Predicts\nPatient Survival in Pancreatic Ductal Adenocarcinoma","volume":"11","author":"Katsuta","year":"2021","journal-title":"Am. 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Nutr."},{"issue":"23","key":"2026080709082615400_cit38","doi-asserted-by":"publisher","first-page":"12918","DOI":"10.3390\/ijms252312918","article-title":"The Role of\nFetuin-A in Tumor Cell Growth, Prognosis,\nand Dissemination","volume":"25","author":"Odiase","year":"2024","journal-title":"Int. J. Mol. Sci."},{"issue":"7","key":"2026080709082615400_cit39","doi-asserted-by":"publisher","first-page":"2976","DOI":"10.3390\/molecules28072976","article-title":"Rac1: A Regulator of Cell Migration\nand a Potential Target for Cancer Therapy","volume":"28","author":"Ma","year":"2023","journal-title":"Molecules"},{"issue":"16","key":"2026080709082615400_cit40","doi-asserted-by":"publisher","first-page":"2999","DOI":"10.1007\/s00018-017-2511-3","article-title":"Vinculin in Cell-Cell\nand Cell-Matrix Adhesions","volume":"74","author":"Bays","year":"2017","journal-title":"Cell. Mol. 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Cancer"},{"issue":"24","key":"2026080709082615400_cit45","doi-asserted-by":"publisher","first-page":"3177","DOI":"10.3390\/diagnostics15243177","article-title":"Analytical Validation of a Serum Biomarker Signature for Detection\nof Early-Stage Pancreatic Ductal Adenocarcinoma","volume":"15","author":"Pescatore","year":"2025","journal-title":"Diagnostics"},{"issue":"3","key":"2026080709082615400_cit46","doi-asserted-by":"publisher","first-page":"676","DOI":"10.3390\/ijms20030676","article-title":"Inflammation and Pancreatic Cancer:\nFocus on Metabolism, Cytokines, and Immunity","volume":"20","author":"Padoan","year":"2019","journal-title":"Int. J. Mol. 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