{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T14:28:21Z","timestamp":1788532101448,"version":"build-2803163510"},"reference-count":35,"publisher":"American Chemical Society (ACS)","issue":"9","license":[{"start":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T00:00:00Z","timestamp":1786320000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T00:00:00Z","timestamp":1786320000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T00:00:00Z","timestamp":1786320000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82404839"],"award-info":[{"award-number":["82404839"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Chinese Medicine Biomics and Innovative Drug Research and Development Laboratory","award":["zyyyjs2024019"],"award-info":[{"award-number":["zyyyjs2024019"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,9,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Colon cancer (CC) is one of the malignant tumors with high incidence and mortality rates worldwide, necessitating innovative diagnostic tools to improve early detection and management. In this study, we developed a large-scale metabolome relative quantitative method workflow using ultrahigh-performance liquid chromatography coupled with Q-TRAP mass spectrometry to identify novel biomarkers in the serum of CC patients. This method enables the detection of 776 metabolic features, spanning 16 chemical classes and 63 metabolic pathways. All detected features were evaluated by multiple identification criteria and assigned confidence scores to ensure annotation reliability. Through validation and application of this method to clinical samples, we compared the serum metabolic profiles of CC patients with those of healthy controls and screened 72 significantly altered metabolites. Pathway enrichment analysis revealed perturbations in tryptophan metabolism, arginine biosynthesis, and the TCA cycle. Notably, three differential metabolites (indole, tryptophan, and xanthurenic acid) were identified that could potentially serve as diagnostic biomarkers with area under the curve values exceeding 0.9. Our large-scale metabolome relative quantitative method demonstrates applicability in identifying potential metabolite biomarkers for CC and provides a promising tool for both basic and clinical metabolomics research.<\/jats:p>","DOI":"10.1021\/acs.jproteome.6c00263","type":"journal-article","created":{"date-parts":[[2026,8,10]],"date-time":"2026-08-10T10:01:55Z","timestamp":1786356115000},"page":"4734-4743","source":"Crossref","is-referenced-by-count":0,"title":["Development\nof a Large-Scale Serum Metabolome Relative\nQuantitative Method and Application for Metabolic Traits of Colon\nCancer"],"prefix":"10.1021","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-0756-4561","authenticated-orcid":true,"given":"Shuting","family":"Yu","sequence":"first","affiliation":[{"name":"Shanxi University of Chinese Medicine , , ,","place":["Jinzhong, China, 030619"]},{"name":"Shanxi University of Chinese Medicine , , ,","place":["Jinzhong, China, 030619"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guifang","family":"Ye","sequence":"additional","affiliation":[{"name":"Shanxi University of Chinese Medicine , , ,","place":["Jinzhong, China, 030619"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiaxin","family":"Gai","sequence":"additional","affiliation":[{"name":"Shanxi University of Chinese Medicine , , ,","place":["Jinzhong, China, 030619"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui","family":"Wei","sequence":"additional","affiliation":[{"name":"Tianjin University of Traditional Chinese Medicine , , 10 Poyanghu Road , ,","place":["Tianjin, China, 301617"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinru","family":"Huang","sequence":"additional","affiliation":[{"name":"Shanxi University of Chinese Medicine , , ,","place":["Jinzhong, China, 030619"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Runjian","family":"Ren","sequence":"additional","affiliation":[{"name":"Third Hospital of Shanxi Medical University, Tongji Shanxi Hospital , , ,","place":["Taiyuan, China, 030032"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,8,10]]},"reference":[{"issue":"1","key":"2026090409142390900_cit1","doi-asserted-by":"publisher","DOI":"10.1080\/19490976.2025.2479774","article-title":"Human colitis-associated\ncolorectal carcinoma progression is accompanied by dysbiosis with\nenriched pathobionts","volume":"17","author":"Masaadeh","year":"2025","journal-title":"Gut Microbes"},{"issue":"4","key":"2026090409142390900_cit2","doi-asserted-by":"publisher","first-page":"361","DOI":"10.1016\/j.clcc.2023.07.001","article-title":"Colon cancer survivorship\nin patients who have received\nadjuvant chemotherapy","volume":"22","author":"Kesireddy","year":"2023","journal-title":"Clin. Colorectal Cancer"},{"issue":"7","key":"2026090409142390900_cit3","doi-asserted-by":"publisher","first-page":"461","DOI":"10.1002\/jms.3782","article-title":"Training in metabolomics\nresearch. I. Designing the experiment, collecting and extracting samples\nand generating metabolomics data","volume":"51","author":"Barnes","year":"2016","journal-title":"J. Mass Spectrom."},{"issue":"9","key":"2026090409142390900_cit4","doi-asserted-by":"publisher","DOI":"10.1007\/s11306-018-1406-0","article-title":"Metabolomics approach for predicting\nresponse to neoadjuvant chemotherapy for colorectal cancer","volume":"14","author":"Yang","year":"2018","journal-title":"Metabolomics"},{"issue":"6","key":"2026090409142390900_cit5","doi-asserted-by":"publisher","first-page":"813","DOI":"10.1002\/dta.2558","article-title":"Identification\nof new urinary gamma-hydroxybutyric acid markers applying untargeted\nmetabolomics analysis following placebo-controlled administration\nto humans","volume":"11","author":"Steuer","year":"2019","journal-title":"Drug Test. Anal."},{"key":"2026090409142390900_cit6","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2024.465230","article-title":"Validation of two LC\nHRMS methods for large-scale untargeted\nmetabolomics of serum samples: Strategy to establish method fitness-for-purpose","volume":"1732","author":"Grijseels","year":"2024","journal-title":"J. Chromatogr. A"},{"issue":"12","key":"2026090409142390900_cit7","doi-asserted-by":"publisher","first-page":"1897","DOI":"10.1007\/s13361-016-1469-y","article-title":"Untargeted\nmetabolomics\nstrategies-challenges and emerging directions","volume":"27","author":"Schrimpe-Rutledge","year":"2016","journal-title":"J. Am. Soc. Mass Spectrom."},{"key":"2026090409142390900_cit8","doi-asserted-by":"publisher","first-page":"30.2.1","DOI":"10.1002\/0471142727.mb3002s98","article-title":"Targeted\nmetabolomics","volume":"98","author":"Roberts","year":"2012","journal-title":"Curr. Protoc. Mol. 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Proteome\nRes."},{"issue":"1","key":"2026090409142390900_cit18","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-025-85893-4","article-title":"Integrated single-cell\nand bulk RNA sequencing analyses reveal the impact of tryptophan metabolism\non prognosis and immunotherapy in colon cancer","volume":"15","author":"Hu","year":"2025","journal-title":"Sci. Rep."},{"issue":"1","key":"2026090409142390900_cit19","doi-asserted-by":"publisher","DOI":"10.1186\/s12943-024-02164-y","article-title":"Molecular\nmechanisms and therapeutic\nsignificance of Tryptophan Metabolism and signaling in cancer","volume":"23","author":"Yan","year":"2024","journal-title":"Mol. 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Gastroenterol."},{"issue":"3","key":"2026090409142390900_cit33","doi-asserted-by":"publisher","first-page":"552","DOI":"10.1002\/ijc.35183","article-title":"Circulating\ntryptophan-kynurenine pathway metabolites are associated with all-cause\nmortality among patients with stage I-III colorectal cancer","volume":"156","author":"Damerell","year":"2025","journal-title":"Int. J. Cancer"},{"key":"2026090409142390900_cit34","doi-asserted-by":"publisher","first-page":"D1522","DOI":"10.1093\/nar\/gkab1081","article-title":"iProX in 2021: connecting\nproteomics data sharing with big data","volume":"50","author":"Chen","year":"2022","journal-title":"Nucleic\nAcids Res."},{"key":"2026090409142390900_cit35","doi-asserted-by":"publisher","first-page":"D1211","DOI":"10.1093\/nar\/gky869","article-title":"iProX: an integrated proteome resource","volume":"47","author":"Ma","year":"2019","journal-title":"Nucleic Acids Res."}],"container-title":["Journal of Proteome Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acs.jproteome.6c00263","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/jprobs\/article-pdf\/25\/9\/4734\/66469724\/acs.jproteome.6c00263.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/jprobs\/article-pdf\/25\/9\/4734\/66469724\/acs.jproteome.6c00263.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T14:05:59Z","timestamp":1788530759000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/jprobs\/article\/25\/9\/4734\/5251005\/Development-of-a-Large-Scale-Serum-Metabolome"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,10]]},"references-count":35,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2026,8,10]]},"published-print":{"date-parts":[[2026,9,4]]}},"URL":"https:\/\/doi.org\/10.1021\/acs.jproteome.6c00263","relation":{},"ISSN":["1535-3893","1535-3907"],"issn-type":[{"value":"1535-3893","type":"print"},{"value":"1535-3907","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,10]]}}}