{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T14:29:49Z","timestamp":1788532189291,"version":"build-2803163510"},"reference-count":70,"publisher":"American Chemical Society (ACS)","issue":"9","license":[{"start":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T00:00:00Z","timestamp":1787616000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T00:00:00Z","timestamp":1787616000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T00:00:00Z","timestamp":1787616000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"funder":[{"name":"FNRS \u2013 National Fund for Scientific Research","award":["40016808"],"award-info":[{"award-number":["40016808"]}]},{"DOI":"10.13039\/501100006673","name":"Fondation L\u00e9on Fredericq","doi-asserted-by":"crossref","award":["2024-083"],"award-info":[{"award-number":["2024-083"]}],"id":[{"id":"10.13039\/501100006673","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,9,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Glioblastoma (GBM) is the most common primary brain cancer in adults and remains fatal, with a median survival of a few months. There is an urgent need to develop novel therapeutic strategies against this aggressive malignancy. Modern cancer research increasingly focuses on personalized therapies tailored toward unique molecular features of each tumor or patient. In this context, cell surface proteins (CSPs) represent an attractive class of therapeutic targets due to their accessibility and central roles in physiological and pathological processes, making them among the most targeted proteins in current drug development. In this study, promising CSPs were identified through an untargeted proteomics approach using high-resolution mass spectrometry on patient-derived GBM stem-like cell (GSC) cultures, complemented by RNA-seq data and computational database analyses. From this primary discovery, five CSPs, namely PTK7, PTPRZ1, OSMR, CSPG4, and IGDCC4, were selected for detailed investigation. A targeted UHPLC\u2013multiple reaction monitoring (MRM) method was developed and optimized to assess their expression and evaluate their abundance variations across different GSC cultures and cell passage levels. Beyond confirming these CSPs as potential therapeutic targets in GBM, our study demonstrates the value of three-dimensional GSC cultures as robust models for biomarker research and target assessment.<\/jats:p>","DOI":"10.1021\/acs.jproteome.6c00267","type":"journal-article","created":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T01:24:19Z","timestamp":1788225859000},"page":"4744-4756","source":"Crossref","is-referenced-by-count":0,"title":["Identification\nof Glioblastoma Cell Surface Proteins\nand Assessment of Their Expression Across Patient-Derived Stem-Like\nCell Cultures"],"prefix":"10.1021","volume":"25","author":[{"given":"Anna","family":"Laurent","sequence":"first","affiliation":[{"name":"University of Lie\u0300ge , , ,","place":["Lie\u0300ge, Belgium, 4000"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gael","family":"Cobraiville","sequence":"additional","affiliation":[{"name":"University of Lie\u0300ge , , ,","place":["Lie\u0300ge, Belgium, 4000"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adrien","family":"Allard","sequence":"additional","affiliation":[{"name":"University of Lie\u0300ge , , ,","place":["Lie\u0300ge, Belgium, 4000"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Murat Cem","family":"Ko\u0308se","sequence":"additional","affiliation":[{"name":"CHU and University of Lie\u0300ge , , ,","place":["Lie\u0300ge, Belgium, 4000"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arnaud","family":"Lombard","sequence":"additional","affiliation":[{"name":"University of Lie\u0300ge , , ,","place":["Lie\u0300ge, Belgium, 4000"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Celia","family":"Lemoine","sequence":"additional","affiliation":[{"name":"University of Lie\u0300ge , , ,","place":["Lie\u0300ge, Belgium, 4000"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Virginie","family":"Neirinckx","sequence":"additional","affiliation":[{"name":"University of Lie\u0300ge , , ,","place":["Lie\u0300ge, Belgium, 4000"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1453-6282","authenticated-orcid":true,"given":"Marianne","family":"Fillet","sequence":"additional","affiliation":[{"name":"University of Lie\u0300ge , , ,","place":["Lie\u0300ge, Belgium, 4000"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,8,25]]},"reference":[{"key":"2026090409131050500_cit1","doi-asserted-by":"publisher","first-page":"n1560","DOI":"10.1136\/bmj.n1560","article-title":"Glioblastoma: Clinical\nPresentation, Diagnosis, and Management","volume":"374","author":"McKinnon","year":"2021","journal-title":"BMJ"},{"issue":"11","key":"2026090409131050500_cit2","doi-asserted-by":"publisher","first-page":"1437","DOI":"10.1001\/jamaneurol.2014.1701","article-title":"Emerging Therapies\nfor Glioblastoma","volume":"71","author":"Thomas","year":"2014","journal-title":"JAMA Neurol."},{"issue":"5","key":"2026090409131050500_cit3","doi-asserted-by":"publisher","first-page":"3040","DOI":"10.3390\/ijms25053040","article-title":"Glioblastoma:\nAn Update in Pathology, Molecular Mechanisms\nand Biomarkers","volume":"25","author":"Lan","year":"2024","journal-title":"Int. J. Mol. Sci."},{"issue":"8","key":"2026090409131050500_cit4","doi-asserted-by":"publisher","first-page":"1231","DOI":"10.1093\/neuonc\/noab106","article-title":"The 2021 WHO Classification\nof Tumors of the Central\nNervous System: A Summary","volume":"23","author":"Louis","year":"2021","journal-title":"Neuro-Oncol."},{"issue":"12","key":"2026090409131050500_cit5","doi-asserted-by":"publisher","first-page":"2664","DOI":"10.3390\/biomedicines12122664","article-title":"Predictive\nand Prognostic Significance of Molecular Biomarkers in Glioblastoma","volume":"12","author":"Shah","year":"2024","journal-title":"Biomedicines"},{"issue":"10","key":"2026090409131050500_cit6","doi-asserted-by":"publisher","first-page":"997","DOI":"10.1056\/NEJMoa043331","article-title":"MGMT Gene Silencing and Benefit from Temozolomide in Glioblastoma","volume":"352","author":"Hegi","year":"2005","journal-title":"N. Engl. J. Med."},{"issue":"7","key":"2026090409131050500_cit7","doi-asserted-by":"publisher","first-page":"494","DOI":"10.3390\/cells14070494","article-title":"Therapeutic\nTargets in Glioblastoma:\nMolecular Pathways, Emerging Strategies, and Future Directions","volume":"14","author":"Tang","year":"2025","journal-title":"Cells"},{"issue":"11","key":"2026090409131050500_cit8","doi-asserted-by":"publisher","first-page":"2751","DOI":"10.1093\/neuonc\/noaf177","article-title":"Glioblastoma in Adults: A Society for Neuro-Oncology\n(SNO) and European Society of Neuro-Oncology (EANO) Consensus Review\non Current Management and Future Directions","volume":"27","author":"Wen","year":"2025","journal-title":"Neuro-Oncol."},{"issue":"4","key":"2026090409131050500_cit9","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0121314","article-title":"A Mass Spectrometric-Derived\nCell Surface Protein Atlas","volume":"10","author":"Bausch-Fluck","year":"2015","journal-title":"PLoS One"},{"issue":"1","key":"2026090409131050500_cit10","doi-asserted-by":"publisher","first-page":"850","DOI":"10.1186\/s12885-021-08591-0","article-title":"Integration\nof RNA-Seq and Proteomics Data Identifies Glioblastoma Multiforme\nSurfaceome Signature","volume":"21","author":"Syafruddin","year":"2021","journal-title":"BMC Cancer"},{"issue":"1","key":"2026090409131050500_cit11","doi-asserted-by":"publisher","first-page":"84","DOI":"10.1038\/s41592-020-01022-1","article-title":"Cell Surface\nThermal Proteome Profiling Tracks Perturbations and Drug Targets on\nthe Plasma Membrane","volume":"18","author":"Kalxdorf","year":"2021","journal-title":"Nat. Methods"},{"key":"2026090409131050500_cit12","doi-asserted-by":"publisher","first-page":"289","DOI":"10.1007\/978-3-030-15950-4_16","article-title":"Strategies for Membrane Protein\nAnalysis by Mass Spectrometry","volume-title":"Advancements\nof Mass Spectrometry in Biomedical Research","author":"Prados-Rosales","year":"2019"},{"issue":"3","key":"2026090409131050500_cit13","doi-asserted-by":"publisher","first-page":"259","DOI":"10.1080\/14789450.2018.1429924","article-title":"Cell-Surface\nProteomics for the Identification of Novel Therapeutic Targets in\nCancer","volume":"15","author":"Kuhlmann","year":"2018","journal-title":"Expert Rev. Proteomics"},{"issue":"14","key":"2026090409131050500_cit14","doi-asserted-by":"publisher","first-page":"7764","DOI":"10.1073\/pnas.1917947117","article-title":"Broad and Thematic\nRemodeling of the Surfaceome and\nGlycoproteome on Isogenic Cells Transformed with Driving Proliferative\nOncogenes","volume":"117","author":"Leung","year":"2020","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"issue":"1","key":"2026090409131050500_cit15","doi-asserted-by":"publisher","first-page":"19","DOI":"10.1038\/nrd.2016.230","article-title":"A Comprehensive\nMap of Molecular Drug Targets","volume":"16","author":"Santos","year":"2017","journal-title":"Nat. Rev. Drug\nDiscovery"},{"issue":"1","key":"2026090409131050500_cit16","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20220808","article-title":"Bridging the Gap between Tumor and Disease: Innovating\nCancer and Glioma Models","volume":"222","author":"Cirigliano","year":"2025","journal-title":"J. Exp. Med."},{"key":"2026090409131050500_cit17","doi-asserted-by":"publisher","first-page":"120812","DOI":"10.1016\/j.talanta.2020.120812","article-title":"Enhancing Protein Discoverability\nby Data Independent Acquisition Assisted by Ion Mobility Mass Spectrometry","volume":"213","author":"Nys","year":"2020","journal-title":"Talanta"},{"key":"2026090409131050500_cit18","doi-asserted-by":"publisher","first-page":"1630","DOI":"10.1100\/tsw.2002.848","article-title":"Homogenization\nof Mammalian Cultured Cells","volume":"2","author":"Graham","year":"2002","journal-title":"Sci. World J."},{"key":"2026090409131050500_cit19","doi-asserted-by":"publisher","first-page":"341764","DOI":"10.1016\/j.aca.2023.341764","article-title":"Improvement\nof Untargeted Proteomics Workflow for Surfaceome\nProfiling and Its Evaluation through the Implementation of Quality\nControls: Application to Multiple Myeloma","volume":"1279","author":"Gou","year":"2023","journal-title":"Anal.\nChim. Acta"},{"issue":"1","key":"2026090409131050500_cit20","doi-asserted-by":"publisher","first-page":"8","DOI":"10.1002\/pro.4218","article-title":"PANTHER: Making Genome-scale\nPhylogenetics\nAccessible to All","volume":"31","author":"Thomas","year":"2022","journal-title":"Protein Sci."},{"issue":"D1","key":"2026090409131050500_cit21","doi-asserted-by":"publisher","first-page":"D609","DOI":"10.1093\/nar\/gkae1010","article-title":"UniProt:\nThe Universal\nProtein Knowledgebase in 2025","volume":"53","author":"Bateman","year":"2025","journal-title":"Nucleic Acids\nRes."},{"issue":"6340","key":"2026090409131050500_cit22","doi-asserted-by":"publisher","DOI":"10.1126\/science.aal3321","article-title":"A Subcellular Map of the Human Proteome","volume":"356","author":"Thul","year":"2017","journal-title":"Science"},{"key":"2026090409131050500_cit23","doi-asserted-by":"publisher","first-page":"bau012","DOI":"10.1093\/database\/bau012","article-title":"COMPARTMENTS: Unification and Visualization\nof Protein Subcellular Localization Evidence","volume":"2014","author":"Binder","year":"2014","journal-title":"Database"},{"issue":"10","key":"2026090409131050500_cit24","doi-asserted-by":"publisher","first-page":"4885","DOI":"10.1021\/pr300360a","article-title":"Proteomic\nAnalysis Reveals Drug Accessible\nCell Surface N-Glycoproteins of Primary and Established Glioblastoma\nCell Lines","volume":"11","author":"Bock","year":"2012","journal-title":"J. Proteome Res."},{"issue":"5","key":"2026090409131050500_cit25","doi-asserted-by":"publisher","first-page":"516","DOI":"10.1016\/j.cels.2017.03.004","article-title":"A Cell-Surface\nMembrane Protein Signature for\nGlioblastoma","volume":"4","author":"Ghosh","year":"2017","journal-title":"Cell Syst."},{"issue":"3","key":"2026090409131050500_cit26","doi-asserted-by":"publisher","first-page":"btaf060","DOI":"10.1093\/bioinformatics\/btaf060","article-title":"ImmunoTar\u2500Integrative\nPrioritization of Cell Surface Targets for Cancer Immunotherapy","volume":"41","author":"Shraim","year":"2025","journal-title":"Bioinformatics"},{"issue":"1","key":"2026090409131050500_cit27","doi-asserted-by":"publisher","first-page":"1613","DOI":"10.1038\/s41467-022-29154-2","article-title":"The Landscape of Receptor-Mediated Precision Cancer\nCombination Therapy via a Single-Cell Perspective","volume":"13","author":"Ahmadi","year":"2022","journal-title":"Nat. Commun."},{"issue":"5","key":"2026090409131050500_cit28","doi-asserted-by":"publisher","first-page":"2565","DOI":"10.1021\/pr100012p","article-title":"Identification\nof\nCell Surface Glycoprotein Markers for Glioblastoma-Derived Stem-Like\nCells Using a Lectin Microarray and LC\u2013MS\/MS Approach","volume":"9","author":"He","year":"2010","journal-title":"J. Proteome Res."},{"issue":"W1","key":"2026090409131050500_cit29","doi-asserted-by":"publisher","first-page":"W98","DOI":"10.1093\/nar\/gkx247","article-title":"GEPIA: A Web\nServer for Cancer and Normal Gene Expression Profiling and Interactive\nAnalyses","volume":"45","author":"Tang","year":"2017","journal-title":"Nucleic Acids Res."},{"issue":"D1","key":"2026090409131050500_cit30","doi-asserted-by":"publisher","first-page":"D638","DOI":"10.1093\/nar\/gkac1000","article-title":"The STRING\nDatabase in 2023: Protein\u2013Protein Association Networks and\nFunctional Enrichment Analyses for Any Sequenced Genome of Interest","volume":"51","author":"Szklarczyk","year":"2023","journal-title":"Nucleic Acids Res."},{"issue":"1","key":"2026090409131050500_cit31","doi-asserted-by":"publisher","first-page":"184","DOI":"10.1038\/s41698-025-00956-z","article-title":"Defining\nthe Extracellular Matrix for Targeted Immunotherapy\nin Adult and Pediatric Brain Cancer","volume":"9","author":"Day","year":"2025","journal-title":"Npj Precis.\nOncol."},{"issue":"2","key":"2026090409131050500_cit32","doi-asserted-by":"publisher","DOI":"10.1080\/2162402X.2017.1391972","article-title":"Antigenic Expression and Spontaneous Immune Responses\nSupport the Use of a Selected Peptide Set from the IMA950 Glioblastoma\nVaccine for Immunotherapy of Grade II and III Glioma","volume":"7","author":"Dutoit","year":"2018","journal-title":"OncoImmunology"},{"key":"2026090409131050500_cit33","doi-asserted-by":"publisher","first-page":"1448695","DOI":"10.3389\/fonc.2024.1448695","article-title":"PTK7: An Underestimated\nContributor to Human Cancer","volume":"14","author":"Jin","year":"2024","journal-title":"Front. Oncol."},{"issue":"5","key":"2026090409131050500_cit34","doi-asserted-by":"publisher","first-page":"530","DOI":"10.7150\/thno.10824","article-title":"Theranostic Impact of NG2\/CSPG4 Proteoglycan\nin Cancer","volume":"5","author":"Nicolosi","year":"2015","journal-title":"Theranostics"},{"issue":"4","key":"2026090409131050500_cit35","doi-asserted-by":"publisher","first-page":"2585","DOI":"10.3892\/ol.2020.11394","article-title":"Overview of\nthe STAT-3 Signaling\nPathway in Cancer and the Development of Specific Inhibitors","volume":"19","author":"Gu","year":"2020","journal-title":"Oncol. Lett."},{"issue":"7_Supplement","key":"2026090409131050500_cit36","doi-asserted-by":"publisher","first-page":"1539","DOI":"10.1158\/1538-7445.AM2026-1539","article-title":"Abstract 1539:\nHarnessing NOPE: A Key Oncofetal Protein Driving \u03b2-Catenin Signaling\nthrough Peptidic Antigen Receptor Macrophages in Hepatocellular Carcinoma","volume":"86","author":"Ning Leung","year":"2026","journal-title":"Cancer Res."},{"key":"2026090409131050500_cit37","doi-asserted-by":"publisher","first-page":"115649","DOI":"10.1016\/j.intimp.2025.115649","article-title":"Exploring\nthe Role of OSMR in Gastric Cancer: From Oncogenic Functions to Relevant\nImmune Features","volume":"167","author":"Lan","year":"2025","journal-title":"Int. Immunopharmacol."},{"issue":"22","key":"2026090409131050500_cit38","doi-asserted-by":"publisher","first-page":"2309857","DOI":"10.1002\/advs.202309857","article-title":"Hypoxic Exosomal\ncircPLEKHM1-Mediated\nCrosstalk between Tumor Cells and Macrophages Drives Lung Cancer Metastasis","volume":"11","author":"Wang","year":"2024","journal-title":"Adv. Sci."},{"issue":"6","key":"2026090409131050500_cit39","doi-asserted-by":"publisher","first-page":"1248","DOI":"10.1093\/carcin\/bgt494","article-title":"Genome-Wide Unmasking of Epigenetically\nSilenced Genes in Lung Adenocarcinoma from Smokers and Never Smokers","volume":"35","author":"Tessema","year":"2014","journal-title":"Carcinogenesis"},{"issue":"3","key":"2026090409131050500_cit40","doi-asserted-by":"publisher","first-page":"541","DOI":"10.1007\/s12282-022-01332-6","article-title":"Identification of\nKey Tumor Stroma-Associated Transcriptional\nSignatures Correlated with Survival Prognosis and Tumor Progression\nin Breast Cancer","volume":"29","author":"Uddin","year":"2022","journal-title":"Breast Cancer"},{"issue":"1","key":"2026090409131050500_cit41","doi-asserted-by":"publisher","first-page":"4116","DOI":"10.1038\/s41467-020-17885-z","article-title":"OSMR Controls Glioma Stem Cell Respiration\nand Confers Resistance of Glioblastoma to Ionizing Radiation","volume":"11","author":"Sharanek","year":"2020","journal-title":"Nat. Commun."},{"issue":"6","key":"2026090409131050500_cit42","doi-asserted-by":"publisher","first-page":"798","DOI":"10.1038\/nn.4295","article-title":"Control of\nGlioblastoma Tumorigenesis by Feed-Forward Cytokine Signaling","volume":"19","author":"Jahani-Asl","year":"2016","journal-title":"Nat. Neurosci."},{"issue":"6","key":"2026090409131050500_cit43","doi-asserted-by":"publisher","first-page":"779","DOI":"10.1016\/j.ccell.2021.05.002","article-title":"Interactions between\nCancer Cells and Immune Cells Drive Transitions to Mesenchymal-like\nStates in Glioblastoma","volume":"39","author":"Hara","year":"2021","journal-title":"Cancer Cell"},{"issue":"9","key":"2026090409131050500_cit44","doi-asserted-by":"publisher","first-page":"2724","DOI":"10.3390\/ijms19092724","article-title":"The Significance\nof Chondroitin Sulfate Proteoglycan\n4 (CSPG4) in Human Gliomas","volume":"19","author":"Schiffer","year":"2018","journal-title":"Int. J. Mol. Sci."},{"issue":"9","key":"2026090409131050500_cit45","doi-asserted-by":"publisher","first-page":"101042831772428","DOI":"10.1177\/1010428317724282","article-title":"Prognostic Relevance of NG2\/CSPG4,\nCD44 and Ki-67 in Patients with Glioblastoma","volume":"39","author":"Tsidulko","year":"2017","journal-title":"Tumour Biol."},{"issue":"7","key":"2026090409131050500_cit46","doi-asserted-by":"publisher","first-page":"112679","DOI":"10.1016\/j.celrep.2023.112679","article-title":"PTK7 Is\na Positive Allosteric Modulator of GPR133 Signaling in Glioblastoma","volume":"42","author":"Frenster","year":"2023","journal-title":"Cell Rep."},{"issue":"3","key":"2026090409131050500_cit47","doi-asserted-by":"publisher","first-page":"766","DOI":"10.1016\/j.cell.2016.06.041","article-title":"Human SRMAtlas: A Resource of Targeted\nAssays to Quantify the Complete Human Proteome","volume":"166","author":"Kusebauch","year":"2016","journal-title":"Cell"},{"issue":"8","key":"2026090409131050500_cit48","doi-asserted-by":"publisher","first-page":"3737","DOI":"10.1038\/s41596-021-00566-6","article-title":"Tutorial: Best Practices and Considerations for Mass-Spectrometry-Based\nProtein Biomarker Discovery and Validation","volume":"16","author":"Nakayasu","year":"2021","journal-title":"Nat. Protoc."},{"key":"2026090409131050500_cit49","doi-asserted-by":"publisher","first-page":"7","DOI":"10.1016\/j.aca.2017.01.059","article-title":"A Review on Mass Spectrometry-Based\nQuantitative Proteomics:\nTargeted and Data Independent Acquisition","volume":"964","author":"Vidova","year":"2017","journal-title":"Anal.\nChim. Acta"},{"key":"2026090409131050500_cit50","doi-asserted-by":"publisher","first-page":"207","DOI":"10.1007\/978-1-4939-3524-6_12","article-title":"Selecting Optimal\nPeptides for Targeted Proteomic Experiments in Human Plasma Using\nIn Vitro Synthesized Proteins as Analytical Standards","volume-title":"Quantitative Proteomics by Mass Spectrometry","author":"Bollinger","year":"2016"},{"issue":"11","key":"2026090409131050500_cit51","doi-asserted-by":"publisher","first-page":"1399","DOI":"10.1093\/clinchem\/hvac135","article-title":"Validation\nof an LC-MS\/MS Method Using Solid-Phase Extraction for the Quantification\nof 1\u201384 Parathyroid Hormone: Toward a Candidate Reference Measurement\nProcedure","volume":"68","author":"Farre\u0301-Segura","year":"2022","journal-title":"Clin. Chem."},{"key":"2026090409131050500_cit52","doi-asserted-by":"publisher","DOI":"10.26434\/chemrxiv-2024-dw5zg-v2","article-title":"Significant Impact\nof Consumable\nMaterial and Buffer Composition for Low-Cell Number Proteomic Sample\nPreparation","author":"Kune","year":"2024","journal-title":"ChemRxiv"},{"key":"2026090409131050500_cit53","unstructured":"Solubilization database.  CubeBiotechnol; https:\/\/cube-biotech.com\/solubilization-database, (accessed 19th January 2026)."},{"issue":"4","key":"2026090409131050500_cit54","doi-asserted-by":"publisher","first-page":"2054","DOI":"10.1021\/ac504700t","article-title":"High-Sensitivity N-Glycoproteomic\nAnalysis of Mouse Brain Tissue by Protein Extraction with a Mild Detergent\nof N-Dodecyl \u03b2-D-Maltoside","volume":"87","author":"Liu","year":"2015","journal-title":"Anal. Chem."},{"key":"2026090409131050500_cit55","volume-title":"International Council for\nHarmonisation (ICH) Guidelines: validation\nof Analytical Procedures Q2(R2) Step5 - Revison 2","year":"2024"},{"key":"2026090409131050500_cit56","unstructured":"Agilent Technologies.\n          \n          Enough Data Points across\na LC peak; https:\/\/www.agilent.com\/cs\/library\/support\/documents\/FAQ_Approved_PDF_Template_enough_datapoints.pdf, (accessed 17th decembre 2025)."},{"issue":"1","key":"2026090409131050500_cit57","doi-asserted-by":"publisher","first-page":"512","DOI":"10.3892\/ol.2017.6221","article-title":"Epidermal Growth Factor\nReceptor in Glioblastoma","volume":"14","author":"Xu","year":"2017","journal-title":"Oncol. Lett."},{"issue":"6","key":"2026090409131050500_cit58","doi-asserted-by":"publisher","first-page":"1127","DOI":"10.1007\/s00401-022-02506-4","article-title":"The Proteomic\nLandscape of Glioblastoma Recurrence Reveals Novel and Targetable\nImmunoregulatory Drivers","volume":"144","author":"Tatari","year":"2022","journal-title":"Acta Neuropathol."},{"issue":"2","key":"2026090409131050500_cit59","doi-asserted-by":"publisher","first-page":"290","DOI":"10.1093\/neuonc\/noac169","article-title":"Quantitative Proteomic Landscapes of Primary and Recurrent Glioblastoma\nReveal a Protumorigeneic Role for FBXO2-Dependent Glioma-Microenvironment\nInteractions","volume":"25","author":"Buehler","year":"2023","journal-title":"Neuro Oncol."},{"issue":"12","key":"2026090409131050500_cit60","doi-asserted-by":"publisher","first-page":"2184","DOI":"10.1016\/j.cell.2022.04.038","article-title":"GLASS Consortium. Glioma Progression Is Shaped\nby Genetic Evolution and Microenvironment Interactions","volume":"185","author":"Varn","year":"2022","journal-title":"Cell"},{"key":"2026090409131050500_cit61","doi-asserted-by":"publisher","first-page":"20","DOI":"10.1016\/j.ab.2019.04.001","article-title":"NMR Metabolomics for Evaluating Passage\nNumber and Harvesting Effects on Mammalian Cell Metabolome","volume":"576","author":"Abdul-Hamid","year":"2019","journal-title":"Anal. Biochem."},{"issue":"3","key":"2026090409131050500_cit62","doi-asserted-by":"publisher","first-page":"1582","DOI":"10.1021\/acs.jproteome.0c00806","article-title":"Uncovering the Effect\nof Passage\nNumber on HT29 Cell Line Based on the Cell Metabolomic Approach","volume":"20","author":"Cao","year":"2021","journal-title":"J. Proteome Res."},{"issue":"1","key":"2026090409131050500_cit63","doi-asserted-by":"publisher","first-page":"15869","DOI":"10.1038\/s41598-022-19018-6","article-title":"Passage Number Affects Differentiation\nof Sensory Neurons from Human Induced Pluripotent Stem Cells","volume":"12","author":"Cantor","year":"2022","journal-title":"Sci. Rep."},{"issue":"24","key":"2026090409131050500_cit64","doi-asserted-by":"publisher","first-page":"3966","DOI":"10.1016\/j.febslet.2009.10.036","article-title":"Correlation of mRNA\nand Protein in\nComplex Biological Samples","volume":"583","author":"Maier","year":"2009","journal-title":"FEBS Lett."},{"issue":"2","key":"2026090409131050500_cit65","doi-asserted-by":"publisher","first-page":"91","DOI":"10.2174\/1389202911314020003","article-title":"Integrated Analysis of Transcriptomic and Proteomic Data","volume":"14","author":"Haider","year":"2013","journal-title":"Curr. Genomics"},{"issue":"4","key":"2026090409131050500_cit66","doi-asserted-by":"publisher","first-page":"1137","DOI":"10.1016\/j.cell.2024.11.028","article-title":"Global\nOrganelle Profiling Reveals Subcellular Localization\nand Remodeling at Proteome Scale","volume":"188","author":"Hein","year":"2025","journal-title":"Cell"},{"issue":"12","key":"2026090409131050500_cit67","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1010141","article-title":"A Genome-Wide CRISPR\/Cas9\nGene Knockout Screen Identifies Immunoglobulin\nSuperfamily DCC Subclass Member 4 as a Key Host Factor That Promotes\nInfluenza Virus Endocytosis","volume":"17","author":"Song","year":"2021","journal-title":"PLoS Pathog."},{"key":"2026090409131050500_cit68","doi-asserted-by":"publisher","DOI":"10.5772\/intechopen.99493","article-title":"Overview of Primary Cell Culture\nModels in Preclinical Research of Prostate and Bladder Cancer","volume-title":"Cell Culture -- Advanced Technology and Applications\nin Medical and Life Sciences","author":"Killekar","year":"2022"},{"issue":"4","key":"2026090409131050500_cit69","doi-asserted-by":"publisher","first-page":"862","DOI":"10.1021\/pr050420t","article-title":"Differential\nProtein Profiling of Primary versus Immortalized Human RPE Cells Identifies\nExpression Patterns Associated with Cytoskeletal Remodeling and Cell\nSurvival","volume":"5","author":"Alge","year":"2006","journal-title":"J. Proteome Res."},{"issue":"3","key":"2026090409131050500_cit70","doi-asserted-by":"publisher","first-page":"443","DOI":"10.1074\/mcp.M800258-MCP200","article-title":"Comparative Proteomic Phenotyping\nof Cell Lines and Primary Cells\nto Assess Preservation of Cell Type-Specific Functions","volume":"8","author":"Pan","year":"2009","journal-title":"Mol. Cell. Proteomics"}],"container-title":["Journal of Proteome Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acs.jproteome.6c00267","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/jprobs\/article-pdf\/25\/9\/4744\/67409311\/acs.jproteome.6c00267.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/jprobs\/article-pdf\/25\/9\/4744\/67409311\/acs.jproteome.6c00267.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T14:11:15Z","timestamp":1788531075000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/jprobs\/article\/25\/9\/4744\/5329091\/Identification-of-Glioblastoma-Cell-Surface"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,25]]},"references-count":70,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2026,8,25]]},"published-print":{"date-parts":[[2026,9,4]]}},"URL":"https:\/\/doi.org\/10.1021\/acs.jproteome.6c00267","relation":{},"ISSN":["1535-3893","1535-3907"],"issn-type":[{"value":"1535-3893","type":"print"},{"value":"1535-3907","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,25]]}}}