{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T17:16:21Z","timestamp":1786122981237,"version":"build-2736575974"},"reference-count":70,"publisher":"American Chemical Society (ACS)","issue":"8","license":[{"start":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T00:00:00Z","timestamp":1785369600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T00:00:00Z","timestamp":1785369600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T00:00:00Z","timestamp":1785369600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"funder":[{"DOI":"10.13039\/501100002701","name":"Ministry of Education","doi-asserted-by":"publisher","award":["NRF-2021R1I1A3050027"],"award-info":[{"award-number":["NRF-2021R1I1A3050027"]}],"id":[{"id":"10.13039\/501100002701","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002701","name":"Ministry of Education","doi-asserted-by":"publisher","award":["RS-2023-00219563"],"award-info":[{"award-number":["RS-2023-00219563"]}],"id":[{"id":"10.13039\/501100002701","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002701","name":"Ministry of Education","doi-asserted-by":"publisher","award":["RS-2024-00348103"],"award-info":[{"award-number":["RS-2024-00348103"]}],"id":[{"id":"10.13039\/501100002701","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002560","name":"Soonchunhyang University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002560","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,8,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Extracellular vesicles (EVs) produced by probiotic bacteria are increasingly recognized as crucial mediators of host-microbe communication. However, the molecular composition and biological interpretation of bacterial EV proteomes are heavily influenced by the isolation methods. In this study, we systematically compared ultracentrifugation (UC) and size exclusion chromatography (SEC) for isolating EVs from Lactobacillus reuteri, assessing their impact on EV yield, purity, and proteomic profiles. Although UC yielded significantly more EVs than SEC, it also resulted in lower purity, as evidenced by higher protein contamination and a decreased particle-to-protein ratio. In contrast, SEC improved EV purity by approximately 6.45-fold, effectively removing non-vesicular proteins. Our quantitative proteomics analysis identified 670 in UC-EVs and 858 in SEC-EVs.-- UC-EVs were primarily enriched with cytosolic metabolic enzymes, ribosomal proteins, and components associated with macromolecular complexes, indicating cosedimentation artifacts during UC. Conversely, SEC-EVs exhibited selective enrichment of membrane-associated and cell-wall-modifying proteins, reflecting their origin from envelope remodeling processes. Notably, SEC-EVs contained several proteins, including NLP\/P60, peptidoglycan hydrolases, and lipoproteins linked to anti-inflammatory activities. Overall, our findings illustrate that EV proteomes are highly dependent on the isolation method and highlight SEC as a superior approach for enhancing proteomic specificity and biological interpretability in bacterial EV research.<\/jats:p>","DOI":"10.1021\/acs.jproteome.6c00305","type":"journal-article","created":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T13:19:49Z","timestamp":1785849589000},"page":"4333-4346","source":"Crossref","is-referenced-by-count":0,"title":["Impact of\nSize Exclusion Chromatography and Ultracentrifugation\non Purity and Proteomic Profiles of Extracellular Vesicles Derived\nfrom\n                    <i>Lactobacillus reuteri<\/i>"],"prefix":"10.1021","volume":"25","author":[{"given":"Eunbi","family":"Jo","sequence":"first","affiliation":[{"name":"Soonchunhyang University , , ,","place":["Cheonan, Republic of Korea, 31151"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haekang","family":"Yang","sequence":"additional","affiliation":[{"name":"Soonchunhyang University , , ,","place":["Cheonan, Republic of Korea, 31151"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Soyeon","family":"Lee","sequence":"additional","affiliation":[{"name":"Soonchunhyang University , , ,","place":["Cheonan, Republic of Korea, 31151"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chul Won","family":"Seo","sequence":"additional","affiliation":[{"name":"Soonchunhyang University , , ,","place":["Cheonan, Republic of Korea, 31151"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Woocheol","family":"Jung","sequence":"additional","affiliation":[{"name":"Soonchunhyang University , , ,","place":["Cheonan, Republic of Korea, 31151"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoon-Hee","family":"Shin","sequence":"additional","affiliation":[{"name":"Korea Institute of Science and Technology , , ,","place":["Seoul, Republic of Korea, 02792"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1289-311X","authenticated-orcid":true,"given":"Hyun-Mee","family":"Park","sequence":"additional","affiliation":[{"name":"Korea Institute of Science and Technology , , ,","place":["Seoul, Republic of Korea, 02792"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yeon-Seo","family":"Lee","sequence":"additional","affiliation":[{"name":"Soonchunhyang University , , ,","place":["Cheonan, Republic of Korea, 31151"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eun-Young","family":"Lee","sequence":"additional","affiliation":[{"name":"Soonchunhyang University , , ,","place":["Cheonan, Republic of Korea, 31151"]},{"name":"Soonchunhyang University , , ,","place":["Cheonan, Republic of Korea, 31151"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ho-Yeon","family":"Song","sequence":"additional","affiliation":[{"name":"Soonchunhyang University , , ,","place":["Cheonan, Republic of Korea, 31151"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jae Heon","family":"Kim","sequence":"additional","affiliation":[{"name":"Soonchunhyang University Seoul Hospital, Soonchunhyang University Medical College , , ,","place":["Seoul, Republic of Korea, 04401"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2516-5616","authenticated-orcid":true,"given":"Dongsic","family":"Choi","sequence":"additional","affiliation":[{"name":"Soonchunhyang University , , ,","place":["Cheonan, Republic of Korea, 31151"]},{"name":"Soonchunhyang University , , ,","place":["Cheonan, Republic of Korea, 31151"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,7,30]]},"reference":[{"key":"2026080709090635600_cit1","doi-asserted-by":"publisher","first-page":"1042189","DOI":"10.3389\/fphar.2022.1042189","article-title":"Gut microbiome and human health: Exploring how the probiotic genus\nLactobacillus modulate immune responses","volume":"13","author":"Rastogi","year":"2022","journal-title":"Front.\nPharmacol."},{"key":"2026080709090635600_cit2","doi-asserted-by":"publisher","first-page":"757","DOI":"10.3389\/fmicb.2018.00757","article-title":"Role of\nLactobacillus reuteri in\nhuman health and diseases","volume":"9","author":"Mu","year":"2018","journal-title":"Front. Microbiol."},{"key":"2026080709090635600_cit3","doi-asserted-by":"publisher","first-page":"840245","DOI":"10.3389\/fimmu.2022.840245","article-title":"Lactobacillus spp.\nfor gastrointestinal health: current\nand future perspectives","volume":"13","author":"Dempsey","year":"2022","journal-title":"Front. Immunol."},{"issue":"1","key":"2026080709090635600_cit4","doi-asserted-by":"publisher","first-page":"1070","DOI":"10.1186\/1471-2164-15-1070","article-title":"Comparative genomics\nof Lactobacillus crispatus suggests\nnovel mechanisms for the competitive exclusion of Gardnerella vaginalis","volume":"15","author":"Ojala","year":"2014","journal-title":"BMC Genomics"},{"key":"2026080709090635600_cit5","doi-asserted-by":"publisher","first-page":"616713","DOI":"10.3389\/fimmu.2021.616713","article-title":"Modulatory\neffects\nof probiotics during pathogenic infections with emphasis on immune\nregulation","volume":"12","author":"Raheem","year":"2021","journal-title":"Front. Immunol."},{"issue":"1","key":"2026080709090635600_cit6","doi-asserted-by":"publisher","first-page":"23","DOI":"10.1186\/s12575-021-00160-w","article-title":"Evaluation\nof the Function of Probiotics,\nEmphasizing the Role of their Binding to the Intestinal Epithelium\nin the Stability and their Effects on the Immune System","volume":"23","author":"Javanshir","year":"2021","journal-title":"Biol. Proced. Online"},{"issue":"3","key":"2026080709090635600_cit7","doi-asserted-by":"publisher","first-page":"166","DOI":"10.1016\/j.anaerobe.2008.02.001","article-title":"Human-derived probiotic Lactobacillus\nreuteri demonstrate\nantimicrobial activities targeting diverse enteric bacterial pathogens","volume":"14","author":"Spinler","year":"2008","journal-title":"Anaerobe"},{"key":"2026080709090635600_cit8","doi-asserted-by":"publisher","first-page":"118955","DOI":"10.1016\/j.biopha.2025.118955","article-title":"Proteomics of extracellular vesicles\nderived from Lactobacillus: Implications in gut immune homeostasis\nand therapeutic potential","volume":"194","author":"Lee","year":"2026","journal-title":"Biomed. Pharmacother."},{"issue":"1","key":"2026080709090635600_cit9","doi-asserted-by":"publisher","first-page":"15","DOI":"10.1007\/s12602-021-09791-7","article-title":"Targeted delivery\nof probiotics: perspectives on research and commercialization","volume":"14","author":"Yoha","year":"2022","journal-title":"Probiotics Antimicrob. Proteins"},{"issue":"5","key":"2026080709090635600_cit10","doi-asserted-by":"publisher","first-page":"1038","DOI":"10.3390\/nu11051038","article-title":"The immunomodulatory\nproperties of extracellular vesicles derived from probiotics: a novel\napproach for the management of gastrointestinal diseases","volume":"11","author":"Molina-Tijeras","year":"2019","journal-title":"Nutrients"},{"issue":"7","key":"2026080709090635600_cit11","doi-asserted-by":"publisher","first-page":"415","DOI":"10.1038\/s41579-023-00875-5","article-title":"Composition and functions\nof bacterial membrane vesicles","volume":"21","author":"Toyofuku","year":"2023","journal-title":"Nat. Rev. Microbiol."},{"issue":"1","key":"2026080709090635600_cit12","doi-asserted-by":"publisher","first-page":"2427311","DOI":"10.1080\/19490976.2024.2427311","article-title":"Lactic acid bacteria derived extracellular vesicles: emerging bioactive\nnanoparticles in modulating host health","volume":"16","author":"Li","year":"2024","journal-title":"Gut\nMicrobes"},{"issue":"1","key":"2026080709090635600_cit13","doi-asserted-by":"publisher","first-page":"877","DOI":"10.1038\/s41598-018-37120-6","article-title":"Isolation\nand characterization of Lactobacillus-derived membrane vesicles","volume":"9","author":"Dean","year":"2019","journal-title":"Sci. Rep."},{"key":"2026080709090635600_cit14","doi-asserted-by":"publisher","first-page":"136685","DOI":"10.1016\/j.foodchem.2023.136685","article-title":"Proteomic\ncharacterization of extracellular vesicles derived from lactic acid\nbacteria","volume":"427","author":"Lee","year":"2023","journal-title":"Food Chem."},{"issue":"1","key":"2026080709090635600_cit15","doi-asserted-by":"publisher","first-page":"224","DOI":"10.4014\/jmb.2308.08017","article-title":"Isolation and proteomic analysis\nof extracellular vesicles from Lactobacillus\nsalivarius SNK-6","volume":"34","author":"Huang","year":"2024","journal-title":"J. Microbiol. Biotechnol."},{"issue":"1","key":"2026080709090635600_cit16","doi-asserted-by":"publisher","first-page":"25","DOI":"10.1186\/s40104-020-00532-4","article-title":"Lactobacillus reuteri-derived\nextracellular vesicles maintain intestinal immune homeostasis against\nlipopolysaccharide-induced inflammatory responses in broilers","volume":"12","author":"Hu","year":"2021","journal-title":"J. Anim. Sci. Biotechnol."},{"key":"2026080709090635600_cit17","doi-asserted-by":"publisher","first-page":"969","DOI":"10.1016\/j.jconrel.2023.12.011","article-title":"A cell-free, biomimetic\nhydrogel based on probiotic membrane vesicles ameliorates wound healing","volume":"365","author":"Kuhn","year":"2024","journal-title":"J. Control Releaese"},{"issue":"1","key":"2026080709090635600_cit18","doi-asserted-by":"publisher","first-page":"1163","DOI":"10.1038\/s41598-023-27959-9","article-title":"Membrane vesicles\nreleased by Lacticaseibacillus casei BL23 inhibit the biofilm formation\nof Salmonella Enteritidis","volume":"13","author":"da Silva Barreira","year":"2023","journal-title":"Sci. Rep."},{"key":"2026080709090635600_cit19","doi-asserted-by":"publisher","first-page":"1032202","DOI":"10.3389\/fmicb.2022.1032202","article-title":"Extracellular\nmembrane vesicles from Limosilactobacillus reuteri\nstrengthen the intestinal epithelial integrity, modulate cytokine\nresponses and antagonize activation of TRPV1","volume":"13","author":"Pang","year":"2022","journal-title":"Front. Microbiol."},{"issue":"1","key":"2026080709090635600_cit20","doi-asserted-by":"publisher","first-page":"28","DOI":"10.1038\/s41522-024-00502-y","article-title":"Extracellular\nvesicles from vaginal Gardnerella vaginalis and Mobiluncus mulieris\ncontain distinct proteomic cargo and induce inflammatory pathways","volume":"10","author":"Joseph","year":"2024","journal-title":"Npj Biofilms Microbiomes"},{"key":"2026080709090635600_cit21","doi-asserted-by":"publisher","DOI":"10.4014\/jmb.2506.06011","article-title":"Standardizing bacterial extracellular\nvesicle purification:\na call for consensus","volume":"35","author":"Choi","year":"2025","journal-title":"J. Microbiol. Biotechnol."},{"issue":"3","key":"2026080709090635600_cit22","doi-asserted-by":"publisher","first-page":"1058","DOI":"10.3390\/ijms26031058","article-title":"A simplified\nmethod for the isolation\nof extracellular vesicles from probiotic bacteria and their characterization","volume":"26","author":"Sawant","year":"2025","journal-title":"Int. J. Mol. Sci."},{"issue":"11","key":"2026080709090635600_cit23","doi-asserted-by":"publisher","first-page":"3368","DOI":"10.1002\/bit.28522","article-title":"High performance anion exchange chromatography purification\nof probiotic bacterial extracellular vesicles enhances purity and\nanti-inflammatory efficacy","volume":"120","author":"Pirolli","year":"2023","journal-title":"Biotechnol. Bioeng."},{"issue":"3","key":"2026080709090635600_cit24","doi-asserted-by":"publisher","DOI":"10.1128\/Spectrum.01273-21","article-title":"Considerations\nfor the analysis of bacterial membrane\nvesicles: methods of vesicle production and quantification can influence\nbiological and experimental outcomes","volume":"9","author":"Bitto","year":"2021","journal-title":"Microbiol.\nSpectrum."},{"issue":"1","key":"2026080709090635600_cit25","doi-asserted-by":"publisher","first-page":"1324731","DOI":"10.1080\/20013078.2017.1324731","article-title":"Isolation of membrane vesicles from prokaryotes: a\ntechnical and biological comparison reveals heterogeneity","volume":"6","author":"Dauros Singorenko","year":"2017","journal-title":"J. Extracell. Vesicles"},{"issue":"15","key":"2026080709090635600_cit26","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.abg8335","article-title":"Glucocorticoid-induced loss of beneficial gut bacterial\nextracellular vesicles is associated with the pathogenesis of osteonecrosis","volume":"8","author":"Chen","year":"2022","journal-title":"Sci. Adv."},{"issue":"5","key":"2026080709090635600_cit27","doi-asserted-by":"publisher","DOI":"10.1128\/mbio.02375-22","article-title":"Spontaneous prophage induction contributes\nto the production of membrane vesicles by the gram-positive bacterium\nLacticaseibacillus casei BL23","volume":"13","author":"da Silva Barreira","year":"2022","journal-title":"MBio"},{"issue":"1","key":"2026080709090635600_cit28","doi-asserted-by":"publisher","first-page":"12","DOI":"10.3390\/proteomes13010012","article-title":"Proteomics\nof extracellular\nvesicles: recent updates, challenges and limitations","volume":"13","author":"Singh","year":"2025","journal-title":"Proteomes"},{"issue":"3","key":"2026080709090635600_cit29","doi-asserted-by":"publisher","first-page":"447","DOI":"10.20517\/evcna.2023.14","article-title":"Advances in extracellular\nvesicle\nisolation methods: a path towards cell-type specific EV isolation","volume":"4","author":"Shami-Shah","year":"2023","journal-title":"Extracell. Vesicles Circ. Nucleic Acids"},{"issue":"8","key":"2026080709090635600_cit30","doi-asserted-by":"publisher","first-page":"1800156","DOI":"10.1002\/pmic.201800156","article-title":"Optimizing size\nexclusion chromatography for extracellular vesicle enrichment and\nproteomic analysis from clinically relevant samples","volume":"19","author":"Lane","year":"2019","journal-title":"Proteomics"},{"issue":"3","key":"2026080709090635600_cit31","doi-asserted-by":"publisher","first-page":"100055","DOI":"10.1016\/j.crmeth.2021.100055","article-title":"A size-exclusion-based\napproach for purifying extracellular vesicles from human plasma","volume":"1","author":"Vanderboom","year":"2021","journal-title":"Cell Rep. Methods"},{"issue":"1","key":"2026080709090635600_cit32","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/s12967-017-1374-6","article-title":"Comparison\nof membrane affinity-based method with size-exclusion chromatography\nfor isolation of exosome-like vesicles from human plasma","volume":"16","author":"Stranska","year":"2018","journal-title":"J. Transl. Med."},{"issue":"1","key":"2026080709090635600_cit33","doi-asserted-by":"publisher","first-page":"104","DOI":"10.1186\/s12967-021-02775-9","article-title":"Extracellular vesicles\nisolated by size-exclusion chromatography present suitability for\nRNomics analysis in plasma","volume":"19","author":"Yang","year":"2021","journal-title":"J. Transl. Med."},{"issue":"4","key":"2026080709090635600_cit34","doi-asserted-by":"publisher","first-page":"100944","DOI":"10.1016\/j.mcpro.2025.100944","article-title":"Metabolic\nreprogramming into a glycolysis phenotype\ninduced by extracellular vesicles derived from prostate cancer cells","volume":"24","author":"Lee","year":"2025","journal-title":"Mol. Cell. Proteomics"},{"key":"2026080709090635600_cit35","doi-asserted-by":"publisher","first-page":"1040","DOI":"10.3389\/fmicb.2017.01040","article-title":"Detection\nand physicochemical characterization of membrane vesicles (MVs) of\nLactobacillus reuteri DSM 17938","volume":"8","author":"Grande","year":"2017","journal-title":"Front. Microbiol."},{"key":"2026080709090635600_cit36","doi-asserted-by":"publisher","first-page":"94908083","DOI":"10.26599\/NR.2025.94908083","article-title":"Bacteria extracellular\nvesicles derived from Lactobacillus reuteri delivering intrinsic miR-21a-5p\nto accelerate diabetic wound healing","volume":"18","author":"Li","year":"2025","journal-title":"Nano Res."},{"issue":"1","key":"2026080709090635600_cit37","doi-asserted-by":"publisher","first-page":"19861","DOI":"10.3402\/jev.v2i0.19861","article-title":"How pure are your vesicles?","volume":"2","author":"Webber","year":"2013","journal-title":"J. Extracell. Vesicles"},{"issue":"17","key":"2026080709090635600_cit38","doi-asserted-by":"publisher","first-page":"9272","DOI":"10.3390\/ijms25179272","article-title":"Loss of Lipooligosaccharide Synthesis in Acinetobacter\nbaumannii produces changes in outer membrane vesicle protein content","volume":"25","author":"Cano-Castan\u0303o","year":"2024","journal-title":"Int. J. Mol. Sci."},{"issue":"1","key":"2026080709090635600_cit39","doi-asserted-by":"publisher","first-page":"1632099","DOI":"10.1080\/20013078.2019.1632099","article-title":"Analysis of the Escherichia coli extracellular vesicle proteome identifies markers\nof purity and culture conditions","volume":"8","author":"Hong","year":"2019","journal-title":"J. Extracell.\nVesicles"},{"issue":"8","key":"2026080709090635600_cit40","doi-asserted-by":"publisher","first-page":"12357","DOI":"10.1002\/jev2.12357","article-title":"Mass-produced gram-negative\nbacterial outer membrane vesicles activate\ncancer antigen-specific stem-like CD8+ T cells which enables an effective\ncombination immunotherapy with anti-PD-1","volume":"12","author":"Won","year":"2023","journal-title":"J.\nExtracell. Vesicles"},{"issue":"10","key":"2026080709090635600_cit41","doi-asserted-by":"publisher","first-page":"1948","DOI":"10.1074\/mcp.RA118.000644","article-title":"The Impact\nof Oncogenic EGFRvIII on the Proteome of Extracellular\nVesicles Released from Glioblastoma Cells","volume":"17","author":"Choi","year":"2018","journal-title":"Mol.\nCell. Proteomics"},{"issue":"8","key":"2026080709090635600_cit42","doi-asserted-by":"publisher","DOI":"10.1002\/jev2.12091","article-title":"Oncogenic\nRAS drives the CRAF-dependent extracellular vesicle uptake\nmechanism coupled with metastasis","volume":"10","author":"Choi","year":"2021","journal-title":"J. Extracell.\nVesicles"},{"issue":"10","key":"2026080709090635600_cit43","doi-asserted-by":"publisher","DOI":"10.1002\/jex2.58","article-title":"Quantitative proteomics and biological activity of extracellular\nvesicles engineered to express SARS-CoV-2 spike protein","volume":"1","author":"Choi","year":"2022","journal-title":"J. Extracell. Biol."},{"issue":"12","key":"2026080709090635600_cit44","doi-asserted-by":"publisher","first-page":"10","DOI":"10.1128\/iai.00433-20","article-title":"Extracellular vesicle\nbiogenesis and functions in gram-positive\nbacteria","volume":"88","author":"Briaud","year":"2020","journal-title":"Infect. Immun."},{"issue":"1","key":"2026080709090635600_cit45","doi-asserted-by":"publisher","first-page":"81","DOI":"10.1073\/pnas.0408399102","article-title":"Studying multiprotein\ncomplexes by multisignal sedimentation\nvelocity analytical ultracentrifugation","volume":"102","author":"Balbo","year":"2005","journal-title":"Proc.\nNatl. Acad. Sci."},{"issue":"1","key":"2026080709090635600_cit46","doi-asserted-by":"publisher","first-page":"13330","DOI":"10.1038\/s41598-022-17629-7","article-title":"Characterization of extracellular vesicles from Lactiplantibacillus\nplantarum","volume":"12","author":"Kurata","year":"2022","journal-title":"Sci. Rep."},{"issue":"16","key":"2026080709090635600_cit47","doi-asserted-by":"publisher","first-page":"5128","DOI":"10.1039\/C6CS00897F","article-title":"The ENGases:\nversatile biocatalysts for the production of homogeneous N-linked\nglycopeptides and glycoproteins","volume":"46","author":"Fairbanks","year":"2017","journal-title":"Chem. Soc.\nRev."},{"issue":"1","key":"2026080709090635600_cit48","doi-asserted-by":"publisher","first-page":"246","DOI":"10.1038\/s41598-017-17467-y","article-title":"Characterization of novel endo-\u03b2-N-acetylglucosaminidases\nfrom Sphingobacterium species, Beauveria bassiana and Cordyceps militaris\nthat specifically hydrolyze fucose-containing oligosaccharides and\nhuman IgG","volume":"8","author":"Huang","year":"2018","journal-title":"Sci. Rep."},{"key":"2026080709090635600_cit49","doi-asserted-by":"publisher","first-page":"31","DOI":"10.1016\/j.mib.2016.07.015","article-title":"Biogenesis\nof the Gram-positive bacterial cell envelope","volume":"34","author":"Siegel","year":"2016","journal-title":"Curr. Opin. Microbiol."},{"issue":"1","key":"2026080709090635600_cit50","doi-asserted-by":"publisher","first-page":"174","DOI":"10.1128\/MMBR.63.1.174-229.1999","article-title":"Surface proteins of gram-positive\nbacteria and mechanisms\nof their targeting to the cell wall envelope","volume":"63","author":"Navarre","year":"1999","journal-title":"Microbiol. Mol. Biol. Rev."},{"key":"2026080709090635600_cit51","doi-asserted-by":"publisher","first-page":"582582","DOI":"10.3389\/fmicb.2020.582582","article-title":"Lipoproteins\nin Gram-Positive Bacteria: Abundance, Function, Fitness","volume":"11","author":"Nguyen","year":"2020","journal-title":"Front. Microbiol."},{"issue":"4","key":"2026080709090635600_cit52","doi-asserted-by":"publisher","first-page":"586","DOI":"10.1016\/j.biocel.2007.03.018","article-title":"Lytic transglycosylases: bacterial\nspace-making autolysins","volume":"40","author":"Scheurwater","year":"2008","journal-title":"Int. J. Biochem. Cell\nBiol."},{"issue":"49","key":"2026080709090635600_cit53","doi-asserted-by":"publisher","first-page":"17727","DOI":"10.1021\/jacs.7b10170","article-title":"Identification\nof a\nFunctionally Unique Family of Penicillin-Binding Proteins","volume":"139","author":"Welsh","year":"2017","journal-title":"J. Am. Chem. Soc."},{"issue":"5","key":"2026080709090635600_cit54","doi-asserted-by":"publisher","first-page":"747","DOI":"10.1016\/j.str.2018.03.012","article-title":"The molecular basis\nof polysaccharide\nsulfatase activity and a nomenclature for catalytic subsites in this\nclass of enzyme","volume":"26","author":"Hettle","year":"2018","journal-title":"Structure"},{"issue":"35","key":"2026080709090635600_cit55","doi-asserted-by":"publisher","first-page":"24289","DOI":"10.1074\/jbc.M114.573303","article-title":"Characterization of glycosaminoglycan (GAG)\nsulfatases from the human gut symbiont Bacteroides thetaiotaomicron\nreveals the first GAG-specific bacterial endosulfatase","volume":"289","author":"Ulmer","year":"2014","journal-title":"J. Bio. Chem."},{"issue":"4","key":"2026080709090635600_cit56","doi-asserted-by":"publisher","first-page":"1281","DOI":"10.1111\/1751-7915.13972","article-title":"Extracellular vesicle formation in Lactococcus lactis is stimulated\nby prophage-encoded holin\u2013lysin system","volume":"15","author":"Liu","year":"2022","journal-title":"Microb. Biotechnol."},{"issue":"9","key":"2026080709090635600_cit57","doi-asserted-by":"publisher","first-page":"1180","DOI":"10.3390\/v17091180","article-title":"Interplay between bacterial extracellular\nvesicles\nand phages: receptors, mechanisms, and implications","volume":"17","author":"Bo\u0142oz","year":"2025","journal-title":"Viruses"},{"issue":"8","key":"2026080709090635600_cit58","doi-asserted-by":"publisher","first-page":"1695","DOI":"10.1111\/j.1462-5822.2008.01160.x","article-title":"Vesicular\ntransport in Histoplasma capsulatum: an effective mechanism for trans-cell\nwall transfer of proteins and lipids in ascomycetes","volume":"10","author":"Albuquerque","year":"2008","journal-title":"Cell. Microbiol."},{"key":"2026080709090635600_cit59","doi-asserted-by":"publisher","first-page":"785856","DOI":"10.3389\/fmicb.2021.785856","article-title":"Application\nof stem cell-derived extracellular vesicles as an innovative theranostics\nin microbial diseases","volume":"12","author":"Keshavarz Alikhani","year":"2021","journal-title":"Front. Microbiol."},{"issue":"1","key":"2026080709090635600_cit60","doi-asserted-by":"publisher","first-page":"19237","DOI":"10.1038\/s41598-020-75930-9","article-title":"Lactobacillus\ncasei extracellular vesicles stimulate EGFR pathway likely due to\nthe presence of proteins P40 and P75 bound to their surface","volume":"10","author":"Ba\u0308uerl","year":"2020","journal-title":"Sci. Rep."},{"issue":"2","key":"2026080709090635600_cit61","doi-asserted-by":"publisher","first-page":"562","DOI":"10.1053\/j.gastro.2006.11.022","article-title":"Soluble proteins produced by probiotic\nbacteria regulate\nintestinal epithelial cell survival and growth","volume":"132","author":"Yan","year":"2007","journal-title":"J. Gastroenterol."},{"issue":"9","key":"2026080709090635600_cit62","doi-asserted-by":"publisher","DOI":"10.1111\/nmo.14437","article-title":"The secreted\nprotein P75 of Lactobacillus rhamnosus GG regulates the SERT expression\nvia EGFR and NF-\u03baB pathway","volume":"34","author":"Wang","year":"2022","journal-title":"Neurogastroenterol.\nMotil."},{"issue":"5","key":"2026080709090635600_cit63","doi-asserted-by":"publisher","first-page":"442","DOI":"10.1007\/s00535-021-01774-3","article-title":"Lactobacillus\nstress\nprotein GroEL prevents colonic inflammation","volume":"56","author":"Dias","year":"2021","journal-title":"J. Gastroenterol."},{"issue":"23","key":"2026080709090635600_cit64","doi-asserted-by":"publisher","first-page":"9194","DOI":"10.3390\/ijms21239194","article-title":"HSP70-homolog\nDnaK of Pseudomonas\naeruginosa increases the production of IL-27 through expression of\nEBI3 via TLR4-dependent NF-\u03baB and TLR4-independent Akt signaling","volume":"21","author":"Jeon","year":"2020","journal-title":"Int. J. Mol. Sci."},{"issue":"1","key":"2026080709090635600_cit65","doi-asserted-by":"publisher","first-page":"3338","DOI":"10.1038\/s41467-023-38950-3","article-title":"A probiotic\nbi-functional peptidoglycan hydrolase sheds NOD2 ligands to regulate\ngut homeostasis in female mice","volume":"14","author":"Gao","year":"2023","journal-title":"Nat. Commun."},{"issue":"2","key":"2026080709090635600_cit66","doi-asserted-by":"publisher","first-page":"666","DOI":"10.1111\/j.1365-2672.2009.04450.x","article-title":"Intermediate\nchains of exopolysaccharides from Lactobacillus\nrhamnosus RW-9595M increase IL-10 production by macrophages","volume":"108","author":"Bleau","year":"2010","journal-title":"J. Appl. Microbiol."},{"key":"2026080709090635600_cit67","doi-asserted-by":"publisher","first-page":"1491727","DOI":"10.3389\/fmicb.2024.1491727","article-title":"Lactiplantibacillus\nplantarum NMGL2\nexopolysaccharide ameliorates DSS-induced IBD in mice mainly by regulation\nof intestinal tight junction and NF-\u03baB p65 protein expression","volume":"15","author":"Zhou","year":"2024","journal-title":"Front. Microbiol."},{"issue":"3","key":"2026080709090635600_cit68","doi-asserted-by":"publisher","first-page":"502","DOI":"10.1128\/JB.01321-12","article-title":"Impact of Lactobacillus plantarum sortase on target\nprotein sorting, gastrointestinal persistence, and host immune response\nmodulation","volume":"195","author":"Remus","year":"2013","journal-title":"J. Bacteriol."},{"issue":"1","key":"2026080709090635600_cit69","doi-asserted-by":"publisher","first-page":"185","DOI":"10.1128\/AEM.06192-11","article-title":"Functional\nanalysis of Lactobacillus rhamnosus GG pili in relation to adhesion\nand immunomodulatory interactions with intestinal epithelial cells","volume":"78","author":"Lebeer","year":"2012","journal-title":"Appl. Environ. Microbiol."},{"issue":"4","key":"2026080709090635600_cit70","doi-asserted-by":"publisher","first-page":"2160","DOI":"10.1128\/IAI.72.4.2160-2169.2004","article-title":"E. Cell surface-associated elongation\nfactor Tu mediates the attachment of Lactobacillus johnsonii NCC533\n(La1) to human intestinal cells and mucins","volume":"72","author":"Granato","year":"2004","journal-title":"Infect. Immun."}],"container-title":["Journal of Proteome Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acs.jproteome.6c00305","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/jprobs\/article-pdf\/25\/8\/4333\/66334518\/acs.jproteome.6c00305.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/jprobs\/article-pdf\/25\/8\/4333\/66334518\/acs.jproteome.6c00305.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T13:24:18Z","timestamp":1786109058000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/jprobs\/article\/25\/8\/4333\/5237009\/Impact-of-Size-Exclusion-Chromatography-and"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,30]]},"references-count":70,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2026,7,30]]},"published-print":{"date-parts":[[2026,8,7]]}},"URL":"https:\/\/doi.org\/10.1021\/acs.jproteome.6c00305","relation":{},"ISSN":["1535-3893","1535-3907"],"issn-type":[{"value":"1535-3893","type":"print"},{"value":"1535-3907","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,30]]}}}