{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T19:35:33Z","timestamp":1786995333856,"version":"build-2736575974"},"reference-count":43,"publisher":"American Chemical Society (ACS)","issue":"7","license":[{"start":{"date-parts":[[2019,12,30]],"date-time":"2019-12-30T00:00:00Z","timestamp":1577664000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2019,12,30]],"date-time":"2019-12-30T00:00:00Z","timestamp":1577664000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2019,12,30]],"date-time":"2019-12-30T00:00:00Z","timestamp":1577664000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31570346"],"award-info":[{"award-number":["31570346"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31770362"],"award-info":[{"award-number":["31770362"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,7,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Nephrotic syndrome (NS) is one of the leading causes of end-stage renal failure. Unfortunately, reliable surrogate markers for early diagnosing and monitoring the entire progression of NS are as yet absent. A method using UPLC-Q exactive HR-MS was established for the serum metabolomic study of adriamycin-induced nephropathy in rats. Two rat nephropathy models induced by adriamycin were adopted to reflect different degrees of renal damage of early and advanced stages. Then two MPC5 cell models were used to verify the role of proline in the progression of kidney injury. The results showed that seven metabolites such as 14S-HDHA, DPA, and DHA were associated with early renal injury, while 12 metabolites such as tryptophan, linoleyl carnitine, and LysoPC (18:3) reflected the advanced renal disease. At the same time, metabolites including LPE (22:6), LysoPC (22:5), and proline that changed during the whole process of NS were defined as progressive markers. Pathway analysis results showed that fatty acid metabolism, glycerophospholipid metabolism, and amino acids metabolism participated in the occurrence and development of NS. In addition, the change trend of intracellular proline content was consistent with that in serum, and the results were further supported by the detection of the crucial gene PYCRL. This study provides an important basis for searching for diagnostic markers of NS and also provides a methodological reference for early diagnosing and monitoring the pathogenesis of other progressive diseases.<\/jats:p>","DOI":"10.1021\/acs.jproteome.9b00785","type":"journal-article","created":{"date-parts":[[2019,12,30]],"date-time":"2019-12-30T12:03:20Z","timestamp":1577707400000},"page":"2575-2584","source":"Crossref","is-referenced-by-count":16,"title":["Evaluation of\nInjury Degree of Adriamycin-Induced\nNephropathy in Rats Based on Serum Metabolomics Combined with Proline\nMarker"],"prefix":"10.1021","volume":"19","author":[{"given":"Ai-Ping","family":"Li","sequence":"first","affiliation":[{"name":"Modern Research Center for Traditional Chinese Medicine of Shanxi University , ,","place":["Taiyuan, China, 030006"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liu","family":"Yang","sequence":"additional","affiliation":[{"name":"Modern Research Center for Traditional Chinese Medicine of Shanxi University , ,","place":["Taiyuan, China, 030006"]},{"name":"College of Chemistry and Chemical Engineering of Shanxi University , ,","place":["Taiyuan, China, 030006"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li-Chao","family":"Zhang","sequence":"additional","affiliation":[{"name":"Institutes of Biomedical Sciences of Shanxi University , ,","place":["Taiyuan, China, 030006"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sheng-Sheng","family":"He","sequence":"additional","affiliation":[{"name":"Modern Research Center for Traditional Chinese Medicine of Shanxi University , ,","place":["Taiyuan, China, 030006"]},{"name":"College of Chemistry and Chemical Engineering of Shanxi University , ,","place":["Taiyuan, China, 030006"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin-Ping","family":"Jia","sequence":"additional","affiliation":[{"name":"Scientific Instrument Center of Shanxi University , ,","place":["Taiyuan, China, 030006"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0020-3364","authenticated-orcid":true,"given":"Xue-Mei","family":"Qin","sequence":"additional","affiliation":[{"name":"Modern Research Center for Traditional Chinese Medicine of Shanxi University , ,","place":["Taiyuan, China, 030006"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2019,12,30]]},"reference":[{"issue":"6","key":"2026081714240684100_cit1","first-page":"479","article-title":"Diagnosis\nand Management of Nephrotic Syndrome in Adults","volume":"93","author":"Kodner","year":"2016","journal-title":"Am. 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Invest."},{"issue":"1","key":"2026081714240684100_cit4","doi-asserted-by":"publisher","first-page":"35","DOI":"10.3831\/KPI.2019.22.004","article-title":"Renoprotective\nEffect of Against Proteinuria and Apoptosis\nInduced by Adriamycin in Rat","volume":"22","author":"Yazd","year":"2019","journal-title":"J. pharmacopuncture."},{"issue":"2","key":"2026081714240684100_cit5","first-page":"360","article-title":"Evaluation\nof injury degree of rat nephropathy model induced by doxorubicin based\non metabolomics","volume":"49","author":"Zhang","year":"2018","journal-title":"Chin. Tradit. Herbal. Drugs."},{"issue":"2","key":"2026081714240684100_cit6","doi-asserted-by":"publisher","first-page":"F213","DOI":"10.1152\/ajprenal.90421.2008","article-title":"Inducible\nrodent models of acquired podocyte diseases, American journal of physiology.\nAm","volume":"296","author":"Pippin","year":"2009","journal-title":"J. Physiol. Renal. 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