{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T22:14:39Z","timestamp":1792016079478,"version":"4.4.0"},"reference-count":45,"publisher":"American Chemical Society (ACS)","issue":"23","license":[{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"funder":[{"DOI":"10.13039\/100000865","name":"Bill and Melinda Gates Foundation","doi-asserted-by":"publisher","award":["INV-051765"],"award-info":[{"award-number":["INV-051765"]}],"id":[{"id":"10.13039\/100000865","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,6,17]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Accurate malaria staging is vital for treatment decisions and monitoring of transmission. Because mature Plasmodium falciparum parasites sequester in deep vasculature, noninvasive diagnostics must target circulating ring stages and transmissible gametocyte stages. Label-free optical detection is ideal as it probes intrinsic biochemical signatures without exogenous reagents. We present a population-level Raman spectroscopy framework coupled with interpretable machine learning to define biochemical signatures of infected red blood cells. By utilizing confocal and spatially offset Raman spectroscopy (SORS) on synchronized cultures, we captured aggregate biochemical alterations associated with parasite development. SHapley Additive exPlanations (SHAP) analysis identified discriminative spectral regions, specifically hemoglobin-associated vibrational modes, and composite biochemical features. To assess translational feasibility, we demonstrated sensitivity to infection signatures beneath a skin-mimetic phantom, confirming subsurface detectability under tissue-like scattering conditions. These results provide a rigorous biochemical basis for stage-specific Raman classification, laying the groundwork for noninvasive, stage-aware diagnostics capable of identifying the infection and transmission potential.<\/jats:p>","DOI":"10.1021\/acs.nanolett.6c00910","type":"journal-article","created":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T20:22:20Z","timestamp":1780345340000},"page":"7635-7644","source":"Crossref","is-referenced-by-count":2,"title":["Population-Level\nRaman Biochemical Staging of Malaria\nin Human Red Blood Cells Using Interpretable Machine Learning"],"prefix":"10.1021","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2534-0768","authenticated-orcid":true,"given":"Lintong","family":"Wu","sequence":"first","affiliation":[{"name":"Johns Hopkins University , , , ,","place":["Baltimore, Maryland, United States, 21218"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-5409-1561","authenticated-orcid":true,"given":"Anoushka","family":"Gupta","sequence":"additional","affiliation":[{"name":"Johns Hopkins University , , , ,","place":["Baltimore, Maryland, United States, 21218"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9848-0142","authenticated-orcid":true,"given":"Abhai K.","family":"Tripathi","sequence":"additional","affiliation":[{"name":"Johns Hopkins University , , , ,","place":["Baltimore, Maryland, United States, 21205"]},{"name":"Johns Hopkins University , , , ,","place":["Baltimore, Maryland, United States, 21205"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0800-0825","authenticated-orcid":true,"given":"Ishan","family":"Barman","sequence":"additional","affiliation":[{"name":"Johns Hopkins University , , , ,","place":["Baltimore, Maryland, United States, 21218"]},{"name":"Johns Hopkins University School of Medicine , , , ,","place":["Baltimore, Maryland, United States, 21287"]},{"name":"Johns Hopkins University School of Medicine , , , ,","place":["Baltimore, Maryland, United States, 21287"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,6,1]]},"reference":[{"key":"2026073018044432300_cit1","doi-asserted-by":"publisher","DOI":"10.1016\/S2666-5247(24)00016-8","article-title":"The 2023 WHO\nworld malaria report","volume":"5","author":"Venkatesan","year":"2024","journal-title":"Lancet Microbe."},{"key":"2026073018044432300_cit2","doi-asserted-by":"publisher","first-page":"36","DOI":"10.1016\/j.trsl.2017.12.004","article-title":"Image analysis\nand machine learning for detecting malaria","volume":"194","author":"Poostchi","year":"2018","journal-title":"Translational Research."},{"key":"2026073018044432300_cit3","doi-asserted-by":"publisher","first-page":"610","DOI":"10.1016\/j.cell.2016.07.055","article-title":"Malaria: Biology\nand\ndisease","volume":"167","author":"Cowman","year":"2016","journal-title":"Cell."},{"key":"2026073018044432300_cit4","doi-asserted-by":"publisher","first-page":"573","DOI":"10.1038\/nrmicro3519","article-title":"Sexual development\nin plasmodium parasites: Knowing\nwhen it\u2019s time to commit","volume":"13","author":"Josling","year":"2015","journal-title":"Nature Reviews\nMicrobiology."},{"key":"2026073018044432300_cit5","doi-asserted-by":"publisher","first-page":"50","DOI":"10.1038\/nature12876","article-title":"A molecular\nmarker of artemisinin-resistant plasmodium\nfalciparum malaria","volume":"505","author":"Ariey","year":"2014","journal-title":"Nature"},{"key":"2026073018044432300_cit6","doi-asserted-by":"publisher","first-page":"377","DOI":"10.1128\/CMR.00051-10","article-title":"Epidemiology and infectivity\nof plasmodium falciparum\nand plasmodium vivax gametocytes in relation to malaria control and\nelimination","volume":"24","author":"Bousema","year":"2011","journal-title":"Clin. 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