{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,31]],"date-time":"2026-10-31T02:34:24Z","timestamp":1793414064267,"version":"4.5.0"},"reference-count":33,"publisher":"American Chemical Society (ACS)","issue":"36","license":[{"start":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T00:00:00Z","timestamp":1788134400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100005825","name":"National Institute of Food and Agriculture","doi-asserted-by":"publisher","award":["2022-08596"],"award-info":[{"award-number":["2022-08596"]}],"id":[{"id":"10.13039\/100005825","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["R35GM156250"],"award-info":[{"award-number":["R35GM156250"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Albert Mossin Physical and Life Sciences Endowment Scholarship"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,9,16]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>DNA-templated silver nanoclusters have regained attention due to recent advances in stability and tunable optical properties. Here, we report a highly regulated DNA-templated silver nanocluster (DFN2) that exhibits strong fluorescence at 561 nm and enables reversible, label-free fluorescence switching governed by nucleic acid hybridization. The fluorescence of DFN2 is efficiently quenched with complementary DNA or RNA and fully restored through invading RNA strand mediated displacement, enabling robust and programmable ON-OFF-ON optical control. We integrated this hybridization-responsive platform with CRISPR-Cas12a to construct a target-activated biosensing system. Upon recognition of a conserved genomic fragment from a foodborne pathogen Listeria monocytogenes, the activated Cas12a cleaves a regulator DNA, initiating a cascade of hybridization and strand-displacement reactions that restore nanocluster fluorescence through an invasive RNA. This strategy exhibits high specificity against conserved genomic regions from other foodborne pathogens. These findings establish DFN2 as programmable, enzyme-responsive optical reporters for rapid, sensitive, and modular point-of-care biosensing.<\/jats:p>","DOI":"10.1021\/acs.nanolett.6c01946","type":"journal-article","created":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T06:51:53Z","timestamp":1788418313000},"page":"11988-11995","source":"Crossref","is-referenced-by-count":2,"title":["Invasive RNA\nPrograms Optical Switching in DNA Nanoclusters\nfor CRISPR Sensing"],"prefix":"10.1021","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-9836-5715","authenticated-orcid":true,"given":"Reggie","family":"Gold","sequence":"first","affiliation":[{"name":"University at Albany, State University of New York , , 1400 Washington Avenue , , ,","place":["Albany, New York, United States, 12222"]},{"name":"University at Albany, State University of New York , , 1400 Washington Avenue , , ,","place":["Albany, New York, United States, 12222"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yusha","family":"Imtiaz","sequence":"additional","affiliation":[{"name":"University at Albany, State University of New York , , 1400 Washington Avenue , , ,","place":["Albany, New York, United States, 12222"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1539-4108","authenticated-orcid":true,"given":"Vinod","family":"Morya","sequence":"additional","affiliation":[{"name":"University at Albany, State University of New York , , 1400 Washington Avenue , , ,","place":["Albany, New York, United States, 12222"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2578-1339","authenticated-orcid":true,"given":"Ken","family":"Halvorsen","sequence":"additional","affiliation":[{"name":"University at Albany, State University of New York , , 1400 Washington Avenue , , ,","place":["Albany, New York, United States, 12222"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emmett","family":"Hanson","sequence":"additional","affiliation":[{"name":"University at Albany, State University of New York , , 1400 Washington Avenue , , ,","place":["Albany, New York, United States, 12222"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4349-3701","authenticated-orcid":true,"given":"Mehmet V.","family":"Yigit","sequence":"additional","affiliation":[{"name":"University at Albany, State University of New York , , 1400 Washington Avenue , , ,","place":["Albany, New York, United States, 12222"]},{"name":"University at Albany, State University of New York , , 1400 Washington Avenue , , ,","place":["Albany, New York, United States, 12222"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,8,31]]},"reference":[{"issue":"6","key":"2026091608071308600_cit1","doi-asserted-by":"publisher","first-page":"810","DOI":"10.1038\/s41565-024-01612-6","article-title":"A Non-FRET DNA Reporter That Changes Fluorescence Colour\nupon Nuclease Digestion","volume":"19","author":"Hong","year":"2024","journal-title":"Nat. Nanotechnol."},{"issue":"15","key":"2026091608071308600_cit2","doi-asserted-by":"publisher","DOI":"10.1002\/smll.202500784","article-title":"CRISPR-Responsive Reprogrammable\nLabel-Free Fluorescent Nanoclusters for ML-Assisted Pathogenic Genome\nDetection on Solid Substrates","volume":"21","author":"Hanson","year":"2025","journal-title":"Small"},{"issue":"8","key":"2026091608071308600_cit3","doi-asserted-by":"publisher","first-page":"8240","DOI":"10.1021\/acsnano.8b03404","article-title":"Fluorescence\nColor by Data-Driven\nDesign of Genomic Silver Clusters","volume":"12","author":"Copp","year":"2018","journal-title":"ACS Nano"},{"issue":"10","key":"2026091608071308600_cit4","doi-asserted-by":"publisher","first-page":"16322","DOI":"10.1021\/acsnano.2c05390","article-title":"Chemistry-Informed Machine Learning Enables Discovery\nof DNA-Stabilized Silver Nanoclusters with Near-Infrared Fluorescence","volume":"16","author":"Mastracco","year":"2022","journal-title":"ACS Nano"},{"issue":"8","key":"2026091608071308600_cit5","doi-asserted-by":"publisher","first-page":"3106","DOI":"10.1021\/nl101773c","article-title":"A DNA-Silver\nNanocluster Probe That Fluoresces upon Hybridization","volume":"10","author":"Yeh","year":"2010","journal-title":"Nano Lett."},{"issue":"1","key":"2026091608071308600_cit6","doi-asserted-by":"publisher","first-page":"304","DOI":"10.1021\/acs.nanolett.5c05172","article-title":"Concentration Imbalance-Driven Nucleotide Circuit for\nTick-Borne Encephalitis Virus Detection via Hybridization-Responsive\nDNA-Templated Silver Nanoclusters","volume":"26","author":"Wang","year":"2026","journal-title":"Nano Lett."},{"issue":"6","key":"2026091608071308600_cit7","doi-asserted-by":"publisher","first-page":"2323","DOI":"10.1021\/jacsau.4c00291","article-title":"Leveraging Concentration\nImbalance-Driven DNA Circuit as an Operational\nAmplifier to Enhance the Sensitivity of Hepatitis B Virus DNA Detection\nwith Hybridization-Responsive DNA-Templated Silver Nanoclusters","volume":"4","author":"Lv","year":"2024","journal-title":"JACS Au"},{"issue":"12","key":"2026091608071308600_cit8","doi-asserted-by":"publisher","first-page":"833","DOI":"10.1038\/nnano.2010.231","article-title":"The Emerging\nField of RNA Nanotechnology","volume":"5","author":"Guo","year":"2010","journal-title":"Nat. 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