{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T09:13:20Z","timestamp":1788254000809,"version":"build-2803163510"},"reference-count":28,"publisher":"American Chemical Society (ACS)","issue":"33","license":[{"start":{"date-parts":[[2026,8,15]],"date-time":"2026-08-15T00:00:00Z","timestamp":1786752000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,8,15]],"date-time":"2026-08-15T00:00:00Z","timestamp":1786752000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,8,15]],"date-time":"2026-08-15T00:00:00Z","timestamp":1786752000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["22374028"],"award-info":[{"award-number":["22374028"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["22374097"],"award-info":[{"award-number":["22374097"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/na","name":"National Key R&D Program of China","doi-asserted-by":"publisher","award":["2022YFA1305200"],"award-info":[{"award-number":["2022YFA1305200"]}],"id":[{"id":"10.13039\/na","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/na","name":"Inno-vative Research Team of High-level Local Universities in Shanghai","doi-asserted-by":"publisher","award":["SHSMU-ZLCX20212601"],"award-info":[{"award-number":["SHSMU-ZLCX20212601"]}],"id":[{"id":"10.13039\/na","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,8,26]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Tumor-derived extracellular vesicles (EVs) are promising liquid biopsy biomarkers, yet their clinical translation is hindered by the trade-off between enhancement and reproducibility of SERS-based detection. Here, we present a microfluidic SERS platform integrating plasmonic nanobowl arrays that simultaneously achieve a high enhancement factor and signal reproducibility. Periodic nanobowl architectures generate consistent plasmonic hotspots, breaking the typical compromise between sensitivity and uniformity. The aptamer-modified chip enables quantification of EV markers and achieved a calculated analytical limit of detection of 2.5 \u00d7 103 particles mL\u20131 for purified cell line EVs and 1.2 \u00d7 104 particles mL\u20131 for ultrafiltration-pretreated plasma. In a pancreatic cancer mouse model, the platform tracked tumor progression longitudinally, with SERS signals correlating with bioluminescence imaging (r &amp;gt; 0.83). In a pilot clinical study, multimarker analysis achieved 93.33% diagnostic accuracy and effectively monitored chemotherapy response (AUC = 0.80\u20130.96). This robust platform provides a promising tool for EV-based precision oncology.<\/jats:p>","DOI":"10.1021\/acs.nanolett.6c02493","type":"journal-article","created":{"date-parts":[[2026,8,15]],"date-time":"2026-08-15T15:02:01Z","timestamp":1786806121000},"page":"11067-11078","source":"Crossref","is-referenced-by-count":0,"title":["Sensitive and\nReproducible SERS Profiling of Tumor-Derived\nEVs via Uniform Microfluidic Nanobowl Arrays for Pancreatic Cancer\nManagement"],"prefix":"10.1021","volume":"26","author":[{"given":"Ruoke","family":"Wang","sequence":"first","affiliation":[{"name":"Fudan University , , ,","place":["Shanghai, China, 201203"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aipeng","family":"Chen","sequence":"additional","affiliation":[{"name":"Fudan University , , ,","place":["Shanghai, China, 201203"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuqing","family":"Wang","sequence":"additional","affiliation":[{"name":"Fudan University , , ,","place":["Shanghai, China, 201203"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoying","family":"Zhao","sequence":"additional","affiliation":[{"name":"Fudan University , , ,","place":["Shanghai, China, 201203"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0896-6411","authenticated-orcid":true,"given":"Peng","family":"Zhang","sequence":"additional","affiliation":[{"name":"Shanghai Jiao Tong University , , ,","place":["Shanghai, China, 200127"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4583-0043","authenticated-orcid":true,"given":"Xiaoni","family":"Fang","sequence":"additional","affiliation":[{"name":"Fudan University , , ,","place":["Shanghai, China, 201203"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2374-5342","authenticated-orcid":true,"given":"Chaoyong","family":"Yang","sequence":"additional","affiliation":[{"name":"Shanghai Jiao Tong University , , ,","place":["Shanghai, China, 200127"]},{"name":"Xiamen University , , ,","place":["Xiamen, China, 361005"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,8,15]]},"reference":[{"issue":"7950","key":"2026090104152806100_cit1","doi-asserted-by":"publisher","first-page":"168","DOI":"10.1038\/s41586-023-05728-y","article-title":"Microbiota-derived\n3-IAA influences chemotherapy efficacy in pancreatic cancer","volume":"615","author":"Tintelnot","year":"2023","journal-title":"Nature"},{"key":"2026090104152806100_cit2","doi-asserted-by":"publisher","DOI":"10.1016\/j.snb.2019.127516","article-title":"A low-cost nanomaterial-based\nelectrochemical immunosensor on paper\nfor high-sensitivity early detection of pancreatic cancer","volume":"305","author":"Prasad","year":"2020","journal-title":"Sens. Actuators, B"},{"issue":"15","key":"2026090104152806100_cit3","doi-asserted-by":"publisher","first-page":"5113","DOI":"10.1039\/D0AN00704H","article-title":"A new method\nto amplify colorimetric signals of paper-based nanobiosensors for\nsimple and sensitive pancreatic cancer biomarker detection","volume":"145","author":"Prasad","year":"2020","journal-title":"Analyst"},{"issue":"4","key":"2026090104152806100_cit4","doi-asserted-by":"publisher","first-page":"213","DOI":"10.1038\/nrm.2017.125","article-title":"Shedding light on the\ncell biology of extracellular vesicles","volume":"19","author":"van Niel","year":"2018","journal-title":"Nat.\nRev. Mol. Cell Biol."},{"issue":"1","key":"2026090104152806100_cit5","doi-asserted-by":"publisher","first-page":"9","DOI":"10.1038\/s41556-018-0250-9","article-title":"Specificities\nof secretion and uptake of exosomes and other extracellular vesicles\nfor cell-to-cell communication","volume":"21","author":"Mathieu","year":"2019","journal-title":"Nat. Cell Biol."},{"issue":"10","key":"2026090104152806100_cit6","doi-asserted-by":"publisher","first-page":"617","DOI":"10.1038\/s41571-018-0036-9","article-title":"Extracellular\nvesicles in cancer \u2500 implications for future improvements in\ncancer care","volume":"15","author":"Xu","year":"2018","journal-title":"Nat. Rev. Clin. Oncol."},{"issue":"6478","key":"2026090104152806100_cit7","doi-asserted-by":"publisher","DOI":"10.1126\/science.aau6977","article-title":"The biology, function, and biomedical applications\nof exosomes","volume":"367","author":"Kalluri","year":"2020","journal-title":"Science"},{"issue":"4","key":"2026090104152806100_cit8","doi-asserted-by":"publisher","first-page":"1044","DOI":"10.1016\/j.cell.2020.07.009","article-title":"Extracellular Vesicle and Particle\nBiomarkers Define Multiple Human Cancers","volume":"182","author":"Hoshino","year":"2020","journal-title":"Cell"},{"issue":"7","key":"2026090104152806100_cit9","doi-asserted-by":"publisher","first-page":"748","DOI":"10.1038\/s41565-021-00931-2","article-title":"Extracellular vesicles as a next-generation\ndrug delivery platform","volume":"16","author":"Herrmann","year":"2021","journal-title":"Nat. Nanotechnol."},{"issue":"1","key":"2026090104152806100_cit10","doi-asserted-by":"publisher","first-page":"14","DOI":"10.1038\/s41565-024-01774-3","article-title":"Harnessing\nextracellular vesicle heterogeneity for diagnostic and therapeutic\napplications","volume":"20","author":"Carney","year":"2025","journal-title":"Nat. Nanotechnol."},{"issue":"1","key":"2026090104152806100_cit11","doi-asserted-by":"publisher","first-page":"3391","DOI":"10.1038\/s41467-025-58490-2","article-title":"FRET imaging of glycoRNA\non small extracellular vesicles enabling sensitive cancer diagnostics","volume":"16","author":"Ren","year":"2025","journal-title":"Nat. Commun."},{"issue":"6","key":"2026090104152806100_cit12","doi-asserted-by":"publisher","first-page":"438","DOI":"10.1038\/s41551-019-0356-9","article-title":"Ultrasensitive detection\nof circulating\nexosomes with a 3D-nanopatterned microfluidic chip","volume":"3","author":"Zhang","year":"2019","journal-title":"Nat. Biomed. Eng."},{"issue":"21","key":"2026090104152806100_cit13","doi-asserted-by":"publisher","first-page":"5495","DOI":"10.1039\/C9SC00961B","article-title":"Multiplexed\nimmunophenotyping of\ncirculating exosomes on nano-engineered ExoProfile chip towards early\ndiagnosis of cancer","volume":"10","author":"Zhang","year":"2019","journal-title":"Chem. Sci."},{"issue":"3","key":"2026090104152806100_cit14","doi-asserted-by":"publisher","first-page":"183","DOI":"10.1038\/s41551-018-0343-6","article-title":"Low-cost thermophoretic\nprofiling of extracellular-vesicle surface proteins for the early\ndetection and classification of cancers","volume":"3","author":"Liu","year":"2019","journal-title":"Nat.\nBiomed. Eng."},{"issue":"15","key":"2026090104152806100_cit15","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.adf4568","article-title":"Reversible zwitterionic\ncoordination enables rapid, high-yield, and high-purity isolation\nof extracellular vesicles from biofluids","volume":"9","author":"Li","year":"2023","journal-title":"Sci.\nAdv."},{"issue":"2","key":"2026090104152806100_cit16","doi-asserted-by":"publisher","DOI":"10.1002\/jev2.12404","article-title":"Minimal information\nfor studies of extracellular vesicles (MISEV2023): From basic to advanced\napproaches","volume":"13","author":"Welsh","year":"2024","journal-title":"J. Extracell. Vesicles"},{"issue":"547","key":"2026090104152806100_cit17","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.aaz2878","article-title":"Molecular\nand functional extracellular vesicle analysis\nusing nanopatterned microchips monitors tumor progression and metastasis","volume":"12","author":"Zhang","year":"2020","journal-title":"Sci. Transl. Med."},{"issue":"51","key":"2026090104152806100_cit18","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202315113","article-title":"A Magnetically Driven\nTandem Chip Enables Rapid Isolation and Multiplexed Profiling of Extracellular\nVesicles","volume":"62","author":"Zhang","year":"2023","journal-title":"Angew. Chem., Int. Ed."},{"issue":"1","key":"2026090104152806100_cit19","doi-asserted-by":"publisher","first-page":"2292","DOI":"10.1038\/s41467-024-46557-5","article-title":"Thermophoretic\nglycan\nprofiling of extracellular vesicles for triple-negative breast cancer\nmanagement","volume":"15","author":"Li","year":"2024","journal-title":"Nat. Commun."},{"key":"2026090104152806100_cit20","doi-asserted-by":"publisher","first-page":"882","DOI":"10.1038\/s41551-025-01601-7","article-title":"Automated\ndisc device for multiplexed extracellular vesicle isolation\nand labelling from liquid biopsies in cancer diagnostics","volume":"10","author":"Woo","year":"2026","journal-title":"Nat. Biomed. Eng."},{"key":"2026090104152806100_cit21","doi-asserted-by":"publisher","first-page":"212","DOI":"10.1038\/s41592-020-01034-x","article-title":"Exosome detection via\nthe ultrafast-isolation system: EXODUS","volume":"18","author":"Chen","year":"2021","journal-title":"Nat.\nMethods"},{"issue":"47","key":"2026090104152806100_cit22","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.abc1204","article-title":"High-throughput\nsingle-EV liquid biopsy: Rapid, simultaneous, and multiplexed detection\nof nucleic acids, proteins, and their combinations","volume":"6","author":"Zhou","year":"2020","journal-title":"Sci. Adv."},{"issue":"1","key":"2026090104152806100_cit23","doi-asserted-by":"publisher","first-page":"4489","DOI":"10.1038\/s41467-020-18311-0","article-title":"Purification of HCC-specific extracellular vesicles on nanosubstrates\nfor early HCC detection by digital scoring","volume":"11","author":"Sun","year":"2020","journal-title":"Nat. Commun."},{"issue":"4","key":"2026090104152806100_cit24","doi-asserted-by":"publisher","first-page":"1057","DOI":"10.1038\/s41596-024-01082-z","article-title":"High-throughput capture and in situ protein analysis of extracellular\nvesicles by chemical probe-based array","volume":"20","author":"Feng","year":"2025","journal-title":"Nat.\nProtoc."},{"issue":"1","key":"2026090104152806100_cit25","doi-asserted-by":"publisher","first-page":"1644","DOI":"10.1038\/s41467-023-37403-1","article-title":"Single test-based\ndiagnosis\nof multiple cancer types using Exosome-SERS-AI for early stage cancers","volume":"14","author":"Shin","year":"2023","journal-title":"Nat. Commun."},{"issue":"29","key":"2026090104152806100_cit26","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202506744","article-title":"An All-in-One Nanohole\nArray for\nSize-Exclusive Trapping and High-Throughput Digital Counting of Single\nExtracellular Vesicles for Non-Invasive Cancer Screening","volume":"64","author":"Yin","year":"2025","journal-title":"Angew. Chem., Int. Ed."},{"issue":"5","key":"2026090104152806100_cit27","doi-asserted-by":"publisher","first-page":"855","DOI":"10.1039\/D0LC00957A","article-title":"Plasmonic nanobowtiefluidic\ndevice for sensitive detection of glioma extracellular vesicles by\nRaman spectrometry","volume":"21","author":"Jalali","year":"2021","journal-title":"Lab Chip"},{"issue":"19","key":"2026090104152806100_cit28","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.aba2556","article-title":"Exosome-templated\nnanoplasmonics for multiparametric molecular profiling","volume":"6","author":"Wu","year":"2020","journal-title":"Sci. Adv."}],"container-title":["Nano Letters"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acs.nanolett.6c02493","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/nalefd\/article-pdf\/26\/33\/11067\/66604694\/acs.nanolett.6c02493.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/nalefd\/article-pdf\/26\/33\/11067\/66604694\/acs.nanolett.6c02493.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T08:15:33Z","timestamp":1788250533000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/nalefd\/article\/26\/33\/11067\/5259313\/Sensitive-and-Reproducible-SERS-Profiling-of-Tumor"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,15]]},"references-count":28,"journal-issue":{"issue":"33","published-online":{"date-parts":[[2026,8,15]]},"published-print":{"date-parts":[[2026,8,26]]}},"URL":"https:\/\/doi.org\/10.1021\/acs.nanolett.6c02493","relation":{},"ISSN":["1530-6984","1530-6992"],"issn-type":[{"value":"1530-6984","type":"print"},{"value":"1530-6992","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,15]]}}}