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Here, we adopt liquid crystal matrices to amplify chiroptical signals for real-time monitoring of monomer-to-aggregate chiral evolution. In this pyrene-based supramolecular system, \u03c0\u2013\u03c0 stacking and C\u2013H\u00b7\u00b7\u00b7\u03c0 interactions drive the formation of discrete dimers within nematic liquid crystals. The significantly amplified CPL signals with glum values up to 10\u20131 enable direct visualization of chiral transformation. Structural analyses verify that offset face-to-face dimers possess reversed handedness relative to monomers, thus inducing chiroptical inversion. TCNB allosteric encapsulation induces spatial rearrangement of BP dimers within liquid crystal and modulates their helicity-dependent chiroptical activity. A maximum glum value of 0.97 is achieved at the bandgap edge. Such structure reshaping confirms that assembly concentration regulation and molecular intervention enable precise engineering of chiral aggregates, offering fundamental insights into chiral evolution in supramolecular assembly systems.<\/jats:p>","DOI":"10.1021\/acs.nanolett.6c03326","type":"journal-article","created":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T23:07:46Z","timestamp":1788217666000},"page":"11921-11929","source":"Crossref","is-referenced-by-count":0,"title":["Revealing Concentration-Dependent\nAmplification and\nInversion of Circularly Polarized Luminescence in Liquid Crystal Self-Assemblies"],"prefix":"10.1021","volume":"26","author":[{"given":"Shuyuan","family":"Zheng","sequence":"first","affiliation":[{"name":"School of Chemical Science and Engineering , , ,","place":["Shanghai, P. R. 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