{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T19:45:11Z","timestamp":1792784711586,"version":"4.5.0"},"reference-count":35,"publisher":"American Chemical Society (ACS)","issue":"40","license":[{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"funder":[{"DOI":"10.13039\/501100005088","name":"Beijing Municipal Health Commission","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005088","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100020437","name":"Beijing Association for Science and Technology","doi-asserted-by":"publisher","award":["20250899"],"award-info":[{"award-number":["20250899"]}],"id":[{"id":"10.13039\/501100020437","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005090","name":"Beijing Nova Program","doi-asserted-by":"publisher","award":["20230484415"],"award-info":[{"award-number":["20230484415"]}],"id":[{"id":"10.13039\/501100005090","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Beijing Municipal Natural Science Foundation","award":["2254082"],"award-info":[{"award-number":["2254082"]}]},{"name":"Beijing Municipal Natural Science Foundation","award":["L244040"],"award-info":[{"award-number":["L244040"]}]},{"name":"National Basic Research Program of China (973 Program)","award":["2023YFE0117700"],"award-info":[{"award-number":["2023YFE0117700"]}]},{"name":"National Natural Science Foundation of China (NSFC)","award":["52573285"],"award-info":[{"award-number":["52573285"]}],"id":[{"id":"https:\/\/ror.org\/01h0zpd94","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,10,14]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Tuberculosis diagnostics remain challenged by low target abundance, strain diversity, and the need for reliable testing in difficult clinical specimens. Here, we develop an amplification-free nanopore sensing platform based on programmable linear DNA nanohook arrays for direct detection of Mycobacterium tuberculosis (Mtb) DNA. This work presents a dual-target, multisite recognition strategy that simultaneously captures two conserved insertion sequences, IS6110 and IS1081, and converts their binding events into resolvable nanopore electrical signatures. This design improves target recovery and reduces dependence on any single genomic locus, enabling robust detection across diverse Mtb strains. Using this approach, we achieved a limit of detection of 9.5 copies\/\u03bcL and demonstrated accurate identification of Mtb in both sputum and plasma samples without nucleic acid amplification. Our results show that programmable multisite recognition combined with nanopore readout provides a powerful route toward sensitive, sample-flexible tuberculosis diagnostics.<\/jats:p>","DOI":"10.1021\/acs.nanolett.6c03484","type":"journal-article","created":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T13:03:30Z","timestamp":1790859810000},"page":"13938-13947","source":"Crossref","is-referenced-by-count":0,"title":["Programmable Multisite Recognition Enables Amplification-Free\nNanopore Detection of\n                    <i>Mycobacterium tuberculosis<\/i>\n                    in Sputum and Plasma"],"prefix":"10.1021","volume":"26","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-6678-8016","authenticated-orcid":true,"given":"Zhan","family":"Wang","sequence":"first","affiliation":[{"name":"Peking University , , ,","place":["Beijing, China, 100871"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yexiang","family":"Liu","sequence":"additional","affiliation":[{"name":"Peking University , , ,","place":["Beijing, China, 100871"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanrong","family":"Chen","sequence":"additional","affiliation":[{"name":"Peking University , , ,","place":["Beijing, China, 100871"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ke","family":"Jiao","sequence":"additional","affiliation":[{"name":"Peking University , , ,","place":["Beijing, China, 100871"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin","family":"Sun","sequence":"additional","affiliation":[{"name":"Beijing Children\u2019s Hospital, Capital Medical University, National Center for Children\u2019s Health , , ,","place":["Beijing, China, 100045"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenjuan","family":"Nie","sequence":"additional","affiliation":[{"name":"Capital Medical University, Beijing Tuberculosis and Thoracic Tumor Research Institute , , ,","place":["Beijing, China, 101149"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siwei","family":"Wang","sequence":"additional","affiliation":[{"name":"Beijing Children\u2019s Hospital, Capital Medical University, National Center for Children\u2019s Health , , ,","place":["Beijing, China, 100045"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Wei","sequence":"additional","affiliation":[{"name":"Capital Medical University, Beijing Tuberculosis and Thoracic Tumor Research Institute , , ,","place":["Beijing, China, 101149"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3170-0336","authenticated-orcid":true,"given":"Kaikai","family":"Chen","sequence":"additional","affiliation":[{"name":"University of Chinese Academy of Sciences , , ,","place":["Beijing, China, 101408"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weiwei","family":"Jiao","sequence":"additional","affiliation":[{"name":"Beijing Children\u2019s Hospital, Capital Medical University, National Center for Children\u2019s Health , , ,","place":["Beijing, China, 100045"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui","family":"Hu","sequence":"additional","affiliation":[{"name":"Central South University , , , ,","place":["Changsha, Hunan, China, 410083"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3374-6901","authenticated-orcid":true,"given":"Qing","family":"Zhao","sequence":"additional","affiliation":[{"name":"Peking University , , ,","place":["Beijing, China, 100871"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,10,1]]},"reference":[{"key":"2026101408325029600_cit1","volume-title":"Global Tuberculosis\nReport 2025","author":"WHO.","year":"2025"},{"issue":"suppl 4","key":"2026101408325029600_cit2","doi-asserted-by":"publisher","first-page":"S1151","DOI":"10.1093\/infdis\/jir413","article-title":"Advances in the Diagnosis of Pulmonary\nTuberculosis in HIV-Infected and HIV-Uninfected Children","volume":"204","author":"Connell","year":"2011","journal-title":"J. Infect. Dis."},{"issue":"1","key":"2026101408325029600_cit3","doi-asserted-by":"publisher","first-page":"218","DOI":"10.1186\/1471-2334-12-218","article-title":"High Yield\nof Culture-Based Diagnosis\nin a TB-Endemic Setting","volume":"12","author":"Demers","year":"2012","journal-title":"BMC Infect. Dis."},{"issue":"1","key":"2026101408325029600_cit4","doi-asserted-by":"publisher","first-page":"10063","DOI":"10.1038\/ncomms10063","article-title":"Rapid Antibiotic-Resistance\nPredictions from Genome Sequence Data for Staphylococcus Aureus and\nMycobacterium Tuberculosis","volume":"6","author":"Bradley","year":"2015","journal-title":"Nat. Commun."},{"issue":"5","key":"2026101408325029600_cit5","doi-asserted-by":"publisher","first-page":"748","DOI":"10.1038\/s41564-019-0380-2","article-title":"A Systematic\nReview of Biomarkers to Detect Active\nTuberculosis","volume":"4","author":"MacLean","year":"2019","journal-title":"Nat. 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