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Characterization of LnmI revealed ketosynthase (KS)\u2013acyl carrier protein (ACP)\u2013KS domains at the NRPS\u2013PKS interface. Inactivation of the KS domain or ACP domain in vivo abolished LNM production, and the ACP domain can be phosphopantetheinylated in vitro. The LnmI KS\u2013ACP\u2013KS architecture represents a new mechanism for functional crosstalk between NRPS and AT-less type I PKS in the biosynthesis of hybrid peptide\u2013polyketide natural products.<\/jats:p>","DOI":"10.1021\/acs.orglett.6b02033","type":"journal-article","created":{"date-parts":[[2016,8,19]],"date-time":"2016-08-19T15:02:28Z","timestamp":1471618948000},"page":"4288-4291","source":"Crossref","is-referenced-by-count":16,"title":["Characterization of the Ketosynthase and Acyl Carrier\nProtein Domains at the LnmI Nonribosomal Peptide Synthetase\u2013Polyketide\nSynthase Interface for Leinamycin Biosynthesis"],"prefix":"10.1021","volume":"18","author":[{"given":"Yong","family":"Huang","sequence":"first","affiliation":[{"name":"University of Wisconsin , , , ,","place":["Madison, Wisconsin, United States, 53705"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gong-Li","family":"Tang","sequence":"additional","affiliation":[{"name":"University of Wisconsin , , , ,","place":["Madison, Wisconsin, United States, 53705"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guohui","family":"Pan","sequence":"additional","affiliation":[{"name":"The Scripps Research Institute , , , ,","place":["Jupiter, Florida, United States, 33458"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chin-Yuan","family":"Chang","sequence":"additional","affiliation":[{"name":"The Scripps Research Institute , , , ,","place":["Jupiter, Florida, United States, 33458"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ben","family":"Shen","sequence":"additional","affiliation":[{"name":"University of Wisconsin , , , ,","place":["Madison, Wisconsin, United States, 53705"]},{"name":"The Scripps Research Institute , , , ,","place":["Jupiter, Florida, United States, 33458"]},{"name":"The Scripps Research Institute , , , ,","place":["Jupiter, Florida, United States, 33458"]},{"name":"The Scripps Research Institute , , , ,","place":["Jupiter, Florida, United States, 33458"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2016,8,19]]},"reference":[{"key":"2026090119322013200_cit1a","doi-asserted-by":"publisher","first-page":"63","DOI":"10.1126\/science.282.5386.63","volume":"282","author":"Cane","year":"1998","journal-title":"Science"},{"key":"2026090119322013200_cit1b","doi-asserted-by":"publisher","first-page":"4688","DOI":"10.1002\/anie.200806121","volume":"48","author":"Hertweck","year":"2009","journal-title":"Angew. 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