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Despite its versatility as a tool for mechanistic analysis, the broad adoption of RI-NMR has been hindered by the lack of cost- and space-efficient instrument designs, which can be operated with negligible interference to the NMR facility. Herein, we describe an RI apparatus that is compatible with most NMR spectrometers, requires no modification of the NMR instrument or probe, and may be easily transported to and from the laboratory. Accurate reagent delivery is achieved across a range of solvents and temperatures, and samples can be mixed within seconds after injection. The utility of this apparatus is highlighted through four case studies chosen to illustrate different aspects of the instrument.<\/jats:p>","DOI":"10.1021\/acs.orglett.6c02870","type":"journal-article","created":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T20:02:42Z","timestamp":1789675362000},"page":"12159-12164","source":"Crossref","is-referenced-by-count":0,"title":["A Modular Rapid-Injection\nNMR for Structure Elucidation\nand Kinetic Analysis"],"prefix":"10.1021","volume":"28","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-8304-3360","authenticated-orcid":true,"given":"Andrew J.","family":"Ressler","sequence":"first","affiliation":[{"name":"Texas A&M University , , , ,","place":["College Station, Texas, United States, 77843-3255"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6644-5999","authenticated-orcid":true,"given":"Han-Hsiang","family":"Hsu","sequence":"additional","affiliation":[{"name":"Texas A&M University , , , ,","place":["College Station, Texas, United States, 77843-3255"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6336-5566","authenticated-orcid":true,"given":"Andy A.","family":"Thomas","sequence":"additional","affiliation":[{"name":"Texas A&M University , , , ,","place":["College Station, Texas, United States, 77843-3255"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,9,17]]},"reference":[{"key":"2026100208372673100_cit1","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1039\/9781788016483-00001","article-title":"Applications\nof NMR in Polymer Characterization\u2500An Introduction","volume-title":"NMR Methods for Characterization of Synthetic and Natural\nPolymers","author":"Saalwa\u0308chter","year":"2019"},{"key":"2026100208372673100_cit2","doi-asserted-by":"publisher","first-page":"22059","DOI":"10.1016\/S0021-9258(18)45665-7","article-title":"Protein Structure\nDetermination in Solution by NMR Spectroscopy","volume":"265","author":"Wu\u0308thrich","year":"1990","journal-title":"J. Biol. Chem."},{"key":"2026100208372673100_cit3","volume-title":"High-Resolution\nNMR Techniques in Organic Chemistry","author":"Claridge","year":"2016","edition":"3"},{"key":"2026100208372673100_cit4","doi-asserted-by":"publisher","first-page":"277","DOI":"10.1021\/ar50127a004","article-title":"Flow and Stopped-Flow\nNuclear Magnetic\nResonance Investigations of Intermediates in Chemical Reactions","volume":"11","author":"Fyfe","year":"1978","journal-title":"Acc. Chem. Res."},{"key":"2026100208372673100_cit5","doi-asserted-by":"publisher","first-page":"17","DOI":"10.1016\/S0021-9673(00)90489-8","article-title":"Continuous-Flow Nuclear\nMagnetic Resonance","volume":"346","author":"Albert","year":"1985","journal-title":"J. Chromatogr. A"},{"key":"2026100208372673100_cit6","doi-asserted-by":"publisher","first-page":"32","DOI":"10.1038\/s43586-026-00482-7","article-title":"Flow NMR\nfor chemical synthesis","volume":"6","author":"Dumez","year":"2026","journal-title":"Nat. Rev. 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Chem.\nSoc."},{"key":"2026100208372673100_cit10","doi-asserted-by":"publisher","first-page":"310","DOI":"10.1002\/mrc.5395","article-title":"Reaction monitoring\nvia benchtop nuclear magnetic resonance spectroscopy: A practical\ncomparison of on-line stopped-flow and continuous-flow sampling methods","volume":"62","author":"Maschmeyer","year":"2024","journal-title":"Magn. Reson. Chem."},{"key":"2026100208372673100_cit11","doi-asserted-by":"publisher","first-page":"59","DOI":"10.1002\/mrc.1270170114","article-title":"Rapid Injection NMR: A Simple Technique\nfor the Observation of Reactive Intermediates","volume":"17","author":"McGarrity","year":"1981","journal-title":"Org. Magn. Reson."},{"key":"2026100208372673100_cit12","doi-asserted-by":"publisher","first-page":"1157","DOI":"10.1002\/(SICI)1099-0518(19990415)37:8&lt;1157::AID-POLA12&gt;3.0.CO;2-6","article-title":"A Rapid-Injection NMR Study of the Effect of Lithium\nAlkoxides on the Butyllithium-Initiated Polymerization and Propagation\nof Styrene. J. Polym. Sci. Part A","volume":"37","author":"Ogle","year":"1999","journal-title":"Polym. 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Investigation","volume":"23","author":"McGarrity","year":"1982","journal-title":"Tetrahedron\nLett."},{"key":"2026100208372673100_cit16","doi-asserted-by":"publisher","first-page":"462","DOI":"10.1021\/ar00033a002","article-title":"Structural,\nMechanistic, and Theoretical Aspects of Chelation-Controlled Carbonyl\nAddition Reactions","volume":"26","author":"Reetz","year":"1993","journal-title":"Acc. Chem. Res."},{"key":"2026100208372673100_cit17","doi-asserted-by":"publisher","first-page":"7208","DOI":"10.1021\/ja067533d","article-title":"Rapid Injection\nNMR in Mechanistic Organocopper Chemistry. Preparation of the Elusive\nCopper(III) Intermediate","volume":"129","author":"Bertz","year":"2007","journal-title":"J. Am. Chem. 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Commun."},{"key":"2026100208372673100_cit31","doi-asserted-by":"publisher","first-page":"13650","DOI":"10.1021\/ja027744s","article-title":"Rapid-Injection\nNMR Study of Iodo- and Cyano-Gilman\nReagents with 2-Cyclohexenone: Observation of \u03c0-Complexes and\nTheir Rates of Formation","volume":"124","author":"Bertz","year":"2002","journal-title":"J. Am. Chem. Soc."},{"key":"2026100208372673100_cit32","doi-asserted-by":"publisher","DOI":"10.1002\/ejoc.202400095","article-title":"A Simple\nDevice for Automated Mixing\nof Heterogeneous Solid\u2013Liquid Reactions During In\u2013Situ\nMonitoring by NMR Spectroscopy","volume":"27","author":"Gao","year":"2024","journal-title":"Eur. J. Org.\nChem."},{"key":"2026100208372673100_cit33","first-page":"5471","article-title":"Measurements of the interconversion barriers\nbetween sodiopyridine\nisomers have not been made, so we are unable to confirm whether we\nare operating under non-Curtin-Hammett conditions. See: Reich, H.\nJ. What\u2019s Going on with These Lithium Reagents?","volume":"77","year":"2012","journal-title":"J. Org. Chem."},{"key":"2026100208372673100_cit34","doi-asserted-by":"publisher","first-page":"17918","DOI":"10.1021\/jacs.9b09752","article-title":"Mechanistic and Kinetic Studies of the Ring Opening\nMetathesis Polymerization of Norbornenyl Monomers by a Grubbs Third\nGeneration Catalyst","volume":"141","author":"Hyatt","year":"2019","journal-title":"J. Am. Chem. Soc."},{"key":"2026100208372673100_cit35","doi-asserted-by":"publisher","first-page":"4295","DOI":"10.1021\/cs501197w","article-title":"Selective\nBase-Catalyzed Isomerization of Glucose to\nFructose","volume":"4","author":"Liu","year":"2014","journal-title":"ACS Catal."},{"key":"2026100208372673100_cit36","doi-asserted-by":"publisher","first-page":"3162","DOI":"10.1021\/acscatal.5b00316","article-title":"Kinetic\nand Mechanistic Study of\nGlucose Isomerization Using Homogeneous Organic Bro\u0337nsted Base\nCatalysts in Water","volume":"5","author":"Carraher","year":"2015","journal-title":"ACS Catal."},{"key":"2026100208372673100_cit37","unstructured":"Our\nanalysis employed\ndenser sampling and a more detailed fitting equation, leading to a\nmore accurate calculated kobs. The result\nwe obtained is comparable with that previously reported in ref 34."},{"key":"2026100208372673100_cit38","doi-asserted-by":"publisher","first-page":"28","DOI":"10.1016\/j.pnmrs.2022.01.001","article-title":"Mechanistic\nanalysis by NMR spectroscopy: A users guide","volume":"129","author":"Ben-Tal","year":"2022","journal-title":"Prog. Nucl. Magn. Reson. Spectrosc."},{"key":"2026100208372673100_cit39","doi-asserted-by":"publisher","first-page":"1335","DOI":"10.1007\/s00723-024-01676-6","article-title":"Elucidation of Organic\nReaction Mechanisms Using Nuclear\nMagnetic Resonance: A review","volume":"55","author":"Carreras","year":"2024","journal-title":"Appl. Magn. Reson."},{"key":"2026100208372673100_cit40","doi-asserted-by":"publisher","first-page":"2906","DOI":"10.1021\/jo00983a028","article-title":"Preparation\nof Acyclic Isoimides and Their Rearrangement Rates to Imides","volume":"37","author":"Schwarz","year":"1972","journal-title":"J. Org. Chem."},{"key":"2026100208372673100_cit41","doi-asserted-by":"publisher","first-page":"637","DOI":"10.1021\/ja00979a030","article-title":"1,3-Acyl Migrations in Unsaturated Triad (Allyloid)\nSystems. Rearrangements of N-(2,4-Dinitro)benzimidoyl Benzoates","volume":"89","author":"Curtin","year":"1967","journal-title":"J. Am. Chem. Soc."}],"container-title":["Organic Letters"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acs.orglett.6c02870","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/orlef7\/article-pdf\/28\/39\/12159\/68548047\/acs.orglett.6c02870.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/orlef7\/article-pdf\/28\/39\/12159\/68548047\/acs.orglett.6c02870.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T12:49:52Z","timestamp":1790945392000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/orlef7\/article\/28\/39\/12159\/5432239\/A-Modular-Rapid-Injection-NMR-for-Structure"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,17]]},"references-count":41,"journal-issue":{"issue":"39","published-online":{"date-parts":[[2026,9,17]]},"published-print":{"date-parts":[[2026,10,2]]}},"URL":"https:\/\/doi.org\/10.1021\/acs.orglett.6c02870","relation":{},"ISSN":["1523-7060","1523-7052"],"issn-type":[{"value":"1523-7060","type":"print"},{"value":"1523-7052","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,17]]}}}