{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T13:57:27Z","timestamp":1790949447939,"version":"4.1.0"},"reference-count":43,"publisher":"American Chemical Society (ACS)","issue":"39","license":[{"start":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T00:00:00Z","timestamp":1790208000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T00:00:00Z","timestamp":1790208000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T00:00:00Z","timestamp":1790208000000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2021YFA0911500"],"award-info":[{"award-number":["2021YFA0911500"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["22207043"],"award-info":[{"award-number":["22207043"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,10,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Here we describe the construction of an artificial enzyme bearing a biomimetic PLP-like catalytic center within an apo-myoglobin scaffold through covalent incorporation of an abiological salicylaldehyde moiety. By mimicking the substrate activation strategy employed by PLP-dependent enzymes, the engineered catalyst promotes the formation of reactive glycine ester nucleophiles and enables direct \u03b1-functionalization in C\u2013C bond-forming reactions. This work establishes a biocatalytic platform for the synthesis of amino acid derivatives and illustrates how the integration of non-natural catalytic motifs into protein can unlock new-to-nature reactivity beyond the natural enzyme.<\/jats:p>","DOI":"10.1021\/acs.orglett.6c03443","type":"journal-article","created":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T07:02:09Z","timestamp":1790233329000},"page":"12334-12338","source":"Crossref","is-referenced-by-count":0,"title":["Artificial Enzyme\nDesign with an Abiological Salicylaldehyde\nSite Enables Biocatalytic Activation of Glycine Esters"],"prefix":"10.1021","volume":"28","author":[{"given":"Yuhui","family":"Sheng","sequence":"first","affiliation":[{"name":"Jiangnan University , , ,","place":["Wuxi, China, 214122"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7926-118X","authenticated-orcid":true,"given":"Zhi","family":"Zhou","sequence":"additional","affiliation":[{"name":"Jiangnan University , , ,","place":["Wuxi, China, 214122"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,9,24]]},"reference":[{"key":"2026100208373164000_cit1a","doi-asserted-by":"publisher","first-page":"7218","DOI":"10.1038\/s41467-025-62092-3","article-title":"Asymmetric synthesis\nof \u03b2-amino acid derivatives by stereocontrolled C(sp3)-C(sp2) cross-electrophile coupling via radical 1,2-nitrogen\nmigration","volume":"16","author":"Liao","year":"2025","journal-title":"Nat. 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Food Chem."},{"key":"2026100208373164000_cit12a","doi-asserted-by":"publisher","first-page":"2577","DOI":"10.1016\/j.chempr.2024.06.010","article-title":"Repurposing\nmyoglobin into an abiological asymmetric ketoreductase","volume":"10","author":"Zhang","year":"2024","journal-title":"Chem"},{"key":"2026100208373164000_cit12b","doi-asserted-by":"publisher","first-page":"23850","DOI":"10.1021\/jacs.6c02960","article-title":"Enzyme-Catalyzed Stereoselective\nC(sp3)\u2013S Bond Formation via a Dichotomic Carbene\nTransfer Mechanism","volume":"148","author":"Wang","year":"2026","journal-title":"J. Am. Chem. 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Lett."},{"key":"2026100208373164000_cit17b","doi-asserted-by":"publisher","first-page":"e202404312","DOI":"10.1002\/anie.202404312","article-title":"Design and Evolution of an Enzyme for the Asymmetric Michael Addition\nof Cyclic Ketones to Nitroolefins by Enamine Catalysis","volume":"63","author":"Zhu","year":"2024","journal-title":"Angew. Chem., Int. Ed."}],"container-title":["Organic Letters"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acs.orglett.6c03443","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/orlef7\/article-pdf\/28\/39\/12334\/68632965\/acs.orglett.6c03443.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/orlef7\/article-pdf\/28\/39\/12334\/68632965\/acs.orglett.6c03443.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T13:23:23Z","timestamp":1790947403000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/orlef7\/article\/28\/39\/12334\/5437339\/Artificial-Enzyme-Design-with-an-Abiological"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,24]]},"references-count":43,"journal-issue":{"issue":"39","published-online":{"date-parts":[[2026,9,24]]},"published-print":{"date-parts":[[2026,10,2]]}},"URL":"https:\/\/doi.org\/10.1021\/acs.orglett.6c03443","relation":{},"ISSN":["1523-7060","1523-7052"],"issn-type":[{"value":"1523-7060","type":"print"},{"value":"1523-7052","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,24]]}}}