{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T19:43:36Z","timestamp":1792784616114,"version":"4.5.0"},"reference-count":21,"publisher":"American Chemical Society (ACS)","issue":"40","license":[{"start":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T00:00:00Z","timestamp":1789948800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T00:00:00Z","timestamp":1789948800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T00:00:00Z","timestamp":1789948800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["U2106227"],"award-info":[{"award-number":["U2106227"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100014103","name":"Key Technology Research and Development Program of Shandong Province","doi-asserted-by":"publisher","award":["2026CXPT225"],"award-info":[{"award-number":["2026CXPT225"]}],"id":[{"id":"10.13039\/100014103","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Independent Deployment Project of the Institute of Oceanology, Chinese Academy of Sciences","award":["IOCASZZCG009"],"award-info":[{"award-number":["IOCASZZCG009"]}],"id":[{"id":"https:\/\/ror.org\/034t30j35","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,10,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Prenylated indole alkaloids containing a spirooxindole scaffold exhibit diverse biological activities. Here, we report that the flavin-dependent monooxygenase PldC catalyzes the conversion of (+)-premalbrancheamide to 3S-hydroxyindolenine, which undergoes spontaneous rearrangement to form 3R-spirooxindole. In contrast, the homologous enzyme PhqK directly furnishes the 3R-spirooxindole from the same substrate. Structural modeling and site-directed mutagenesis reveal that divergent active-site architectures dictate substrate binding orientation, directing epoxide opening to control the product outcome. These findings highlight how distinct active site architectures drive divergent catalysis to achieve convergent spirooxindole biosynthesis.<\/jats:p>","DOI":"10.1021\/acs.orglett.6c03774","type":"journal-article","created":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T08:02:24Z","timestamp":1789977744000},"page":"12745-12749","source":"Crossref","is-referenced-by-count":0,"title":["Distinct Active-Site\nArchitectures Drive Divergent\nCatalysis for Convergent Spirooxindole Biosynthesis"],"prefix":"10.1021","volume":"28","author":[{"given":"Tiandong","family":"Hu","sequence":"first","affiliation":[{"name":"Shandong University , , , ,","place":["Qingdao, Shandong, China, 266237"]},{"name":"Institute of Oceanology, Chinese Academy of Sciences , , ,","place":["Qingdao, China, 266071"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anran","family":"Fu","sequence":"additional","affiliation":[{"name":"Institute of Oceanology, Chinese Academy of Sciences , , ,","place":["Qingdao, China, 266071"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingyu","family":"Yang","sequence":"additional","affiliation":[{"name":"Shandong University , , , ,","place":["Qingdao, Shandong, China, 266237"]},{"name":"Institute of Oceanology, Chinese Academy of Sciences , , ,","place":["Qingdao, China, 266071"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ting","family":"Lin","sequence":"additional","affiliation":[{"name":"Xiamen University , , , ,","place":["Xiamen, Fujian, China, 361102"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2583-8603","authenticated-orcid":true,"given":"Fan","family":"Yang","sequence":"additional","affiliation":[{"name":"Shanghai Jiao Tong University , , ,","place":["Shanghai, China, 200127"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9459-680X","authenticated-orcid":true,"given":"Wei","family":"Zhang","sequence":"additional","affiliation":[{"name":"Shandong University , , , ,","place":["Qingdao, Shandong, China, 266237"]},{"name":"Institute of Oceanology, Chinese Academy of Sciences , , ,","place":["Qingdao, China, 266071"]},{"name":"University of Chinese Academy of Science , ,","place":["Beijing, China, 100049"]},{"name":"Qingdao Marine Science and Technology Center , , ,","place":["Qingdao, China, 266237"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,9,21]]},"reference":[{"issue":"6","key":"2026100910143110600_cit1","doi-asserted-by":"publisher","first-page":"532","DOI":"10.1039\/C7NP00042A","article-title":"Structural and stereochemical diversity in prenylated\nindole alkaloids containing the bicyclo[2.2.2]diazaoctane\nring system from marine and terrestrial fungi","volume":"35","author":"Klas","year":"2018","journal-title":"Nat. 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