{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T22:04:25Z","timestamp":1789164265352,"version":"build-2803163510"},"reference-count":41,"publisher":"American Chemical Society (ACS)","issue":"11","license":[{"start":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T00:00:00Z","timestamp":1761523200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T00:00:00Z","timestamp":1761523200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T00:00:00Z","timestamp":1761523200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"funder":[{"DOI":"10.13039\/100017604","name":"National Institute of Technology Warangal","doi-asserted-by":"publisher","award":["P1131"],"award-info":[{"award-number":["P1131"]}],"id":[{"id":"10.13039\/100017604","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004541","name":"Ministry of Education, India","doi-asserted-by":"publisher","award":["NA"],"award-info":[{"award-number":["NA"]}],"id":[{"id":"10.13039\/501100004541","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,11,17]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Niclosamide, a well-known anthelmintic drug used against parasitic infections, has gained attention for its potent anticancer and antibacterial activity. However, the use of niclosamide remains limited due to its highly hydrophobic nature and low systemic bioavailability. To address these limitations, we developed a biocompatible drug delivery system to combat the challenges of niclosamide usage. We fabricated niclosamide-loaded polycaprolactone (PCL) and poly(ethylene oxide) (PEO) composite nanofibers and investigated their anticancer and antibacterial applications. Here, PEO enhances drug diffusion and bioavailability, while PCL provides biodegradability and controlled drug release properties. Four different compositions of PEO\u2013PCL nanofibers were synthesized and infused with niclosamide. The synthesized nanofibers were thoroughly characterized by using field emission scanning electron microscopy (FE-SEM), Fourier-transform infrared spectroscopy (FTIR), and energy-dispersive X-ray (EDX) analysis, confirming successful drug incorporation. To test the functionality of fabricated nanofibers, we examined the antibacterial properties using a minimum inhibitory concentration (MIC) and minimum killing concentration (MKC). Interestingly, these nanofibers have a significant activity against Staphylococcus aureus (Gram-positive) but not against Escherichia coli (Gram-negative), suggesting selective action. Furthermore, we assessed the anticancer potential of drug-loaded nanofibers in HeLa cells using a cell cytotoxicity assay. A marked decrease in cell viability was observed when the cells were treated with niclosamide-loaded nanofibers. Collectively, our findings demonstrate that niclosamide-loaded PEO\u2013PCL nanofibers are a promising and efficient drug delivery system with potential applications in antimicrobial and anticancer therapies.<\/jats:p>","DOI":"10.1021\/acsabm.5c01641","type":"journal-article","created":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T05:21:26Z","timestamp":1761542486000},"page":"10334-10343","source":"Crossref","is-referenced-by-count":3,"title":["Engineered Niclosamide Loaded Nanofiber Platforms\nfor Enhanced Antibacterial and Anticancer Efficacy"],"prefix":"10.1021","volume":"8","author":[{"given":"Shahin Mohammed","family":"S K","sequence":"first","affiliation":[{"name":"National Institute of Technology Warangal , , , ,","place":["Warangal, Telangana, India, 506004"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kajal","family":"Sandhu","sequence":"additional","affiliation":[{"name":"National Institute of Technology Warangal , , , ,","place":["Warangal, Telangana, India, 506004"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Harish","family":"G S","sequence":"additional","affiliation":[{"name":"Indian Institute of Technology Roorkee , , , ,","place":["Roorkee, Uttarakhand, India, 247667"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6436-1698","authenticated-orcid":true,"given":"Kiran Sebastian","family":"Kalluvelil","sequence":"additional","affiliation":[{"name":"Indian Institute of Technology Roorkee , , , ,","place":["Roorkee, Uttarakhand, India, 247667"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6885-6409","authenticated-orcid":true,"given":"Unnikrishnan Babukuttan","family":"Sheela","sequence":"additional","affiliation":[{"name":"Dalhousie University , , , ,","place":["Halifax, Nova Scotia, Canada, B3H 4H7"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gopinath","family":"Packirisamy","sequence":"additional","affiliation":[{"name":"Indian Institute of Technology Roorkee , , , ,","place":["Roorkee, Uttarakhand, India, 247667"]},{"name":"Indian Institute of Technology Roorkee , , , ,","place":["Roorkee, Uttarakhand, India, 247667"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2930-7658","authenticated-orcid":true,"given":"S","family":"Chockalingam","sequence":"additional","affiliation":[{"name":"National Institute of Technology Warangal , , , ,","place":["Warangal, Telangana, India, 506004"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2025,10,27]]},"reference":[{"key":"2026073107110966900_cit1","doi-asserted-by":"publisher","first-page":"89","DOI":"10.1016\/j.cellsig.2017.04.001","article-title":"Niclosamide:\nBeyond an antihelminthic 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cancer","volume":"134","author":"Arend","year":"2014","journal-title":"Gynecologic\noncology"},{"key":"2026073107110966900_cit5","doi-asserted-by":"publisher","first-page":"4539","DOI":"10.2147\/IDR.S490998","article-title":"Drug Repurposing: Research Progress\nof Niclosamide and Its Derivatives on Antibacterial Activity","volume":"17","author":"Liu","year":"2024","journal-title":"Infection and Drug Resistance"},{"issue":"1","key":"2026073107110966900_cit6","doi-asserted-by":"publisher","first-page":"21","DOI":"10.1016\/j.it.2014.10.006","article-title":"Intersection\nof mTOR and STAT signaling in immunity","volume":"36","author":"Saleiro","year":"2015","journal-title":"Trends\nin immunology"},{"key":"2026073107110966900_cit7","doi-asserted-by":"publisher","first-page":"408","DOI":"10.3389\/fpls.2018.00408","article-title":"Niclosamide blocks\nrice leaf blight\nby inhibiting biofilm formation of Xanthomonas oryzae","volume":"9","author":"Sahu","year":"2018","journal-title":"Frontiers 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Res."},{"issue":"4","key":"2026073107110966900_cit41","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.02574-18","article-title":"The Anthelmintic\nDrug Niclosamide Synergizes with Colistin and Reverses Colistin Resistance\nin Gram-Negative Bacilli","volume":"63","author":"Domalaon","year":"2019","journal-title":"Antimicrob. 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