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Blending glycerol with silk not only eliminates the brittleness of pure silk films but also enhances flexibility and long-term mechanical stability. Its applicability in the electrochemical detection of Escherichia coli (E. coli) bacteria on a screen-printed electrode system is a testament to the robustness of the substrate. To improve the sensitivity and selectivity of E. coli detection, the working electrode is modified with amine-functionalized WO3 nanorods. The developed flexible biosensor platform selectively detects the targeted E. coli bacteria sample, even in mixed bacterial cultures and fungal strains, with an ultralow detection limit of 0.15 CFU\/mL, and at the same time, it has a wide detection range starting from 0.15 to 1.5 \u00d7 104 CFU\/mL. The repeatability, stability, and specificity studies in real samples affirmatively indicate its potential for usage in clinical applications.<\/jats:p>","DOI":"10.1021\/acsabm.6c00464","type":"journal-article","created":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T22:13:27Z","timestamp":1788214407000},"page":"7987-7998","source":"Crossref","is-referenced-by-count":0,"title":["Fabrication of a Silk-Glycerol-Based Flexible Electrode Decorated with WO3 Nanorods for Ultrasensitive Electrochemical Detection of Bacteria"],"prefix":"10.1021","volume":"9","author":[{"given":"Karri Trinadha","family":"Rao","sequence":"first","affiliation":[{"name":"Indian Institute of Technology, Hyderabad , , , Kandi, ,","place":["Sangareddy, Telangana, India, 502284"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-7077-0187","authenticated-orcid":true,"given":"Pravat Kumar","family":"Sahu","sequence":"additional","affiliation":[{"name":"Indian Institute of Technology, Hyderabad , , , Kandi, ,","place":["Sangareddy, Telangana, India, 502284"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aditya Bhagavathi","family":"Kandala","sequence":"additional","affiliation":[{"name":"Indian Institute of Technology, Hyderabad , , , Kandi, ,","place":["Sangareddy, Telangana, India, 502284"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kokila","family":"G N","sequence":"additional","affiliation":[{"name":"Indian Institute of Technology, Hyderabad , , , Kandi, ,","place":["Sangareddy, Telangana, India, 502284"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rahul","family":"Gangwar","sequence":"additional","affiliation":[{"name":"Indian Institute of Technology, Hyderabad , , , Kandi, ,","place":["Sangareddy, Telangana, India, 502284"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nitisha","family":"Beniwal","sequence":"additional","affiliation":[{"name":"Indian Institute of Technology, Hyderabad , , , Kandi, ,","place":["Sangareddy, Telangana, India, 502284"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3994-6760","authenticated-orcid":true,"given":"Aravind Kumar","family":"Rengan","sequence":"additional","affiliation":[{"name":"Indian Institute of Technology, Hyderabad , , , Kandi, ,","place":["Sangareddy, Telangana, India, 502284"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2643-3854","authenticated-orcid":true,"given":"Subrahmanyam","family":"Challapalli","sequence":"additional","affiliation":[{"name":"Indian Institute of Technology, Hyderabad , , , Kandi, ,","place":["Sangareddy, Telangana, India, 502284"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suresh Kumar","family":"Garlapati","sequence":"additional","affiliation":[{"name":"Indian Institute of Technology, Hyderabad , , , Kandi, ,","place":["Sangareddy, Telangana, India, 502284"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6753-2800","authenticated-orcid":true,"given":"Siva Rama Krishna","family":"Vanjari","sequence":"additional","affiliation":[{"name":"Indian Institute of Technology, Hyderabad , , , Kandi, ,","place":["Sangareddy, Telangana, India, 502284"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,7,28]]},"reference":[{"key":"2026092215071037300_cit1","doi-asserted-by":"publisher","DOI":"10.1016\/j.sna.2025.117053","article-title":"Towards Robust Flexible Electronics: Fabrication Approaches and Ongoing Research Challenges","volume":"395","author":"Omiyale","year":"2025","journal-title":"Sens. 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