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One of the hurdles hindering quantitative studies in this area is the limited stability of AuNCs in common bacterial growth media (BGMs) with respect to flocculation. In this work, we investigated the stability of AuNCs with sizes from \u223c3 to \u223c40 nm in three common BGMs: Tryptic soy broth, Luria\u2013Bertani broth, and Mueller Hinton broth. We find that AuNCs prepared by standard methods, with citrate or citrate\/tannic acid passivation, irreversibly flocculate in all studied BGMs. However, when the freshly synthesized AuNCs are subjected to a surface treatment with N\u2032\u20132-hydroxy-ethylpiperazine-N\u2032\u20132 ethane-sulfonic acid, they become stable in Tryptic soy broth at concentrations up to 500 \u03bcg of Au per mL of medium, for all studied NC sizes. Based on the results of surface enhanced Raman spectroscopy and Fourier transfer infrared studies, we attribute this to the formation of a protective layer on the surface of AuNCs, which prevents the formation of flocculation-inducing protein corona. A structural model explaining the effect is proposed. The stabilization procedure described here paves the way for systematic studies of interactions of AuNCs with bacteria.<\/jats:p>","DOI":"10.1021\/acsabm.6c00618","type":"journal-article","created":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T13:17:37Z","timestamp":1785849457000},"page":"7453-7466","source":"Crossref","is-referenced-by-count":0,"title":["Stabilization of Gold Nanocrystals in Tryptic Soy Broth Bacterial Growth Medium"],"prefix":"10.1021","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-9701-5949","authenticated-orcid":true,"given":"Ivana","family":"Bo\u017eekov\u00e1","sequence":"first","affiliation":[{"name":"Comenius University , , Ilkovi\u010dova 6 , ,","place":["Bratislava, Slovakia, 84215"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7593-8728","authenticated-orcid":true,"given":"Gokul Raj","family":"Mini Rajendran","sequence":"additional","affiliation":[{"name":"Comenius University , , Ilkovi\u010dova 6 , ,","place":["Bratislava, Slovakia, 84215"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monika","family":"Hofbauerov\u00e1","sequence":"additional","affiliation":[{"name":"Institute of Physics Slovak Academy of Sciences , D\u00fabravsk\u00e1 cesta 9 , ,","place":["Bratislava, Slovakia, 84511"]},{"name":"Slovak Academy of Sciences , , D\u00fabravsk\u00e1 cesta 9 , ,","place":["Bratislava, Slovakia, 84104"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alica","family":"Rosov\u00e1","sequence":"additional","affiliation":[{"name":"Institute of Electrical Engineering, Slovak Academy of Sciences , D\u00fabravsk\u00e1 cesta 9 , ,","place":["Bratislava, Slovakia, 84104"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M\u00e1ria","family":"Maz\u00farov\u00e1","sequence":"additional","affiliation":[{"name":"Slovak Medical University in Bratislava , , Limbov\u00e1 12 , ,","place":["Bratislava, Slovakia, 83303"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8300-2674","authenticated-orcid":true,"given":"Miroslava","family":"Hornia\u010dkov\u00e1","sequence":"additional","affiliation":[{"name":"Slovak Medical University in Bratislava , , Limbov\u00e1 12 , ,","place":["Bratislava, Slovakia, 83303"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5495-6428","authenticated-orcid":true,"given":"Milan","family":"S\u00fdkora","sequence":"additional","affiliation":[{"name":"Comenius University , , Ilkovi\u010dova 6 , ,","place":["Bratislava, Slovakia, 84215"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,7,29]]},"reference":[{"issue":"19","key":"2026081707160806200_cit1","doi-asserted-by":"publisher","first-page":"3280","DOI":"10.1002\/anie.200904359","article-title":"Gold Nanoparticles for Biology and Medicine","volume":"49","author":"Giljohann","year":"2010","journal-title":"Angew. 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