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In our system, plasmid-enhanced green fluorescent protein (pEGFP) was chosen as the model plasmid, chitosan (CS) was used to condense the plasmid DNA, poly(lactic-co-glycolic acid) (PLGA) provided sustained-release potential and structural protection for plasmids, and hyaluronic acid (HA) served as a ligand for targeting CD44 receptors on the surface of adult retinal pigment epithelial (ARPE-19) cells. In vitro evaluations revealed that the CS-PLGA\/pEGFP\/HA group exhibited higher cellular uptake, transfection efficiency, and protein expression levels compared with those of free pEGFP and CS-PLGA\/pEGFP groups. Several previous studies have verified the feasibility of intravitreal plasmid delivery. Therefore, this CS-PLGA\/pEGFP\/HA ternary complex is a promising nonviral vector with the potential to deliver plasmids to ocular tissues and enhance protein expression in target cells.<\/jats:p>","DOI":"10.1021\/acsabm.6c00748","type":"journal-article","created":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T19:01:40Z","timestamp":1786474900000},"page":"8088-8099","source":"Crossref","is-referenced-by-count":0,"title":["Construction and In Vitro Evaluation of a CS-PLGA\/pEGFP\/HA Ternary Biodegradable Nanocarrier as a Potential Candidate for Intravitreal Ocular Gene Delivery"],"prefix":"10.1021","volume":"9","author":[{"given":"Tianyi","family":"Wang","sequence":"first","affiliation":[{"name":"Shenyang Pharmaceutical University , , No.103 Wenhua Road , ,","place":["Shenyang, China, 110016"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongqi","family":"Liu","sequence":"additional","affiliation":[{"name":"Shenyang Pharmaceutical University , , No.103 Wenhua Road , ,","place":["Shenyang, China, 110016"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingfei","family":"Xu","sequence":"additional","affiliation":[{"name":"Shenyang Pharmaceutical University , , No.103 Wenhua Road , ,","place":["Shenyang, China, 110016"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shenghao","family":"Gao","sequence":"additional","affiliation":[{"name":"Shenyang Pharmaceutical University , , No.103 Wenhua Road , ,","place":["Shenyang, China, 110016"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shiming","family":"Zhang","sequence":"additional","affiliation":[{"name":"Shenyang Pharmaceutical University , , No.103 Wenhua Road , ,","place":["Shenyang, China, 110016"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8319-5151","authenticated-orcid":true,"given":"Xinggang","family":"Yang","sequence":"additional","affiliation":[{"name":"Shenyang Pharmaceutical University , , No.103 Wenhua Road , ,","place":["Shenyang, China, 110016"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,8,11]]},"reference":[{"issue":"1","key":"2026092215072932900_cit1","doi-asserted-by":"publisher","first-page":"75","DOI":"10.1186\/s11671-025-04234-6","article-title":"Ocular drug delivery systems based on nanotechnology: a comprehensive review for the treatment of eye diseases","volume":"20","author":"Bairagi","year":"2025","journal-title":"Discover Nano"},{"issue":"13","key":"2026092215072932900_cit2","doi-asserted-by":"publisher","first-page":"4490","DOI":"10.1039\/D3BM00505D","article-title":"Nano-based ocular drug delivery systems: an insight into the preclinical\/clinical studies and their potential in the treatment of posterior ocular diseases","volume":"11","author":"Su","year":"2023","journal-title":"Biomater. 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