{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T08:47:42Z","timestamp":1792399662537,"version":"4.4.0"},"reference-count":65,"publisher":"American Chemical Society (ACS)","issue":"20","license":[{"start":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T00:00:00Z","timestamp":1790899200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda??o de Amparo ? Pesquisa do Estado de S?o Paulo","doi-asserted-by":"publisher","award":["2021\/14500-1"],"award-info":[{"award-number":["2021\/14500-1"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda??o de Amparo ? Pesquisa do Estado de S?o Paulo","doi-asserted-by":"publisher","award":["2022\/02189-2"],"award-info":[{"award-number":["2022\/02189-2"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda??o de Amparo ? Pesquisa do Estado de S?o Paulo","doi-asserted-by":"publisher","award":["2023\/07375-1"],"award-info":[{"award-number":["2023\/07375-1"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda??o de Amparo ? Pesquisa do Estado de S?o Paulo","doi-asserted-by":"publisher","award":["2023\/16973-0"],"award-info":[{"award-number":["2023\/16973-0"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda??o de Amparo ? Pesquisa do Estado de S?o Paulo","doi-asserted-by":"publisher","award":["2023\/17867-9"],"award-info":[{"award-number":["2023\/17867-9"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda??o de Amparo ? Pesquisa do Estado de S?o Paulo","doi-asserted-by":"publisher","award":["2024\/03513-3"],"award-info":[{"award-number":["2024\/03513-3"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda??o de Amparo ? Pesquisa do Estado de S?o Paulo","doi-asserted-by":"publisher","award":["2024\/03887-0"],"award-info":[{"award-number":["2024\/03887-0"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda??o de Amparo ? Pesquisa do Estado de S?o Paulo","doi-asserted-by":"publisher","award":["2024\/11383-2"],"award-info":[{"award-number":["2024\/11383-2"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda??o de Amparo ? Pesquisa do Estado de S?o Paulo","doi-asserted-by":"publisher","award":["2024\/15686-0"],"award-info":[{"award-number":["2024\/15686-0"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda??o de Amparo ? Pesquisa do Estado de S?o Paulo","doi-asserted-by":"publisher","award":["2024\/16714-7"],"award-info":[{"award-number":["2024\/16714-7"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda??o de Amparo ? Pesquisa do Estado de S?o Paulo","doi-asserted-by":"publisher","award":["2024\/22821-0"],"award-info":[{"award-number":["2024\/22821-0"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda??o de Amparo ? Pesquisa do Estado de S?o Paulo","doi-asserted-by":"publisher","award":["2025\/00376-8"],"award-info":[{"award-number":["2025\/00376-8"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda??o de Amparo ? Pesquisa do Estado de S?o Paulo","doi-asserted-by":"publisher","award":["2025\/00626-4"],"award-info":[{"award-number":["2025\/00626-4"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda??o de Amparo ? Pesquisa do Estado de S?o Paulo","doi-asserted-by":"publisher","award":["2025\/08727-4"],"award-info":[{"award-number":["2025\/08727-4"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda??o de Amparo ? Pesquisa do Estado de S?o Paulo","doi-asserted-by":"publisher","award":["2025\/12197-0"],"award-info":[{"award-number":["2025\/12197-0"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda??o de Amparo ? Pesquisa do Estado de S?o Paulo","doi-asserted-by":"publisher","award":["2025\/21523-9"],"award-info":[{"award-number":["2025\/21523-9"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,10,19]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Lung cancer remains one of the most lethal malignancies worldwide, with survival rates dropping sharply at advanced stages. Conventional therapies are limited by systemic toxicity and poor selectivity, motivating the development of alternative strategies such as photothermal therapy (PTT). Herein, we investigate gold shell-isolated nanostars (AuNS@SiO2) as photothermal agents for near-infrared (NIR)-triggered cancer treatment in A549 lung cancer cells. The silica shell enhances colloidal stability and protects the plasmonic core, while the nanostar morphology enables efficient NIR light-to-heat conversion. In addition to the photothermal response, AuNS@SiO2 generated singlet oxygen (1O2) under NIR irradiation and was associated with irradiation-dependent changes in the intracellular ROS-related fluorescence, indicating a complementary photochemical oxidative contribution to the photoinduced cellular response. AuNS@SiO2 exhibited minimal cytotoxicity in A549 cells in the absence of irradiation, but induced a concentration-dependent decrease in viability upon NIR photoactivation. Apoptosis\/necrosis assays revealed a shift from viable cells toward populations exhibiting early and late apoptosis-associated features, with minimal necrosis-associated events. Taken together, these findings indicate that the biological response induced by NIR-photoactivated AuNS@SiO2 involves efficient photothermal heating together with a complementary oxidative photochemical contribution and is predominantly associated with apoptosis-like cell death, supporting their potential as effective agents for NIR-based lung cancer therapy.<\/jats:p>","DOI":"10.1021\/acsabm.6c01440","type":"journal-article","created":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T13:01:32Z","timestamp":1790946092000},"page":"9831-9843","source":"Crossref","is-referenced-by-count":0,"title":["Assessment of Apoptosis-like Cell Death in A549 Lung Cancer Cells Induced by Photoactivated Gold Shell-Isolated Nanostars"],"prefix":"10.1021","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-9588-1115","authenticated-orcid":true,"given":"Melissa Nishida","family":"Hirano","sequence":"first","affiliation":[{"name":"S\u00e3o Paulo State University (UNESP) , , , ,","place":["Assis, SP, Brazil, 19806-900"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-1004-1454","authenticated-orcid":true,"given":"Giovanna Eller Silva","family":"Sousa","sequence":"additional","affiliation":[{"name":"S\u00e3o Paulo State University (UNESP) , , , ,","place":["Assis, SP, Brazil, 19806-900"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0998-650X","authenticated-orcid":true,"given":"Andr\u00e9 Satoshi","family":"Ferreira","sequence":"additional","affiliation":[{"name":"S\u00e3o Paulo State University (UNESP) , , , ,","place":["Assis, SP, Brazil, 19806-900"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-3089-060X","authenticated-orcid":true,"given":"Bruna Alves","family":"Martins","sequence":"additional","affiliation":[{"name":"S\u00e3o Paulo State University (UNESP) , , , ,","place":["Assis, SP, Brazil, 19806-900"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thais Soares","family":"de Oliveira","sequence":"additional","affiliation":[{"name":"S\u00e3o Paulo State University (UNESP) , , , ,","place":["Assis, SP, Brazil, 19806-900"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4801-0991","authenticated-orcid":true,"given":"Mirella Boaro","family":"Kobal","sequence":"additional","affiliation":[{"name":"S\u00e3o Paulo State University (UNESP) , , , ,","place":["Assis, SP, Brazil, 19806-900"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabela Batista de Castro","family":"Nunes","sequence":"additional","affiliation":[{"name":"S\u00e3o Paulo State University (UNESP) , , , ,","place":["Assis, SP, Brazil, 19806-900"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7212-6794","authenticated-orcid":true,"given":"Karina Alves","family":"Toledo","sequence":"additional","affiliation":[{"name":"S\u00e3o Paulo State University (UNESP) , , , ,","place":["Assis, SP, Brazil, 19806-900"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4701-6408","authenticated-orcid":true,"given":"Pedro Henrique Benites","family":"Aoki","sequence":"additional","affiliation":[{"name":"S\u00e3o Paulo State University (UNESP) , , , ,","place":["Assis, SP, Brazil, 19806-900"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6590-759X","authenticated-orcid":true,"given":"Sabrina Al\u00e9ssio","family":"Camacho","sequence":"additional","affiliation":[{"name":"S\u00e3o Paulo State University (UNESP) , , , ,","place":["Assis, SP, Brazil, 19806-900"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,10,2]]},"reference":[{"issue":"13","key":"2026101904072714600_cit1","doi-asserted-by":"publisher","first-page":"1583","DOI":"10.1097\/CM9.0000000000002529","article-title":"Global Burden and Trends of Lung Cancer Incidence and Mortality","volume":"136","author":"Li","year":"2023","journal-title":"Chin. Med. J. (Engl.)"},{"issue":"3","key":"2026101904072714600_cit2","doi-asserted-by":"publisher","first-page":"229","DOI":"10.3322\/caac.21834","article-title":"Global Cancer Statistics 2022: GLOBOCAN Estimates of Incidence and Mortality Worldwide for 36 Cancers in 185 Countries","volume":"74","author":"Bray","year":"2024","journal-title":"CA Cancer J. Clin."},{"issue":"1","key":"2026101904072714600_cit3","doi-asserted-by":"publisher","first-page":"39","DOI":"10.1016\/j.jtho.2015.09.009","article-title":"The IASLC Lung Cancer Staging Project: Proposals for Revision of the TNM Stage Groupings in the Forthcoming (Eighth) Edition of the TNM Classification for Lung Cancer","volume":"11","author":"Goldstraw","year":"2016","journal-title":"J. Thorac. Oncol."},{"issue":"2","key":"2026101904072714600_cit4","doi-asserted-by":"publisher","first-page":"270","DOI":"10.1016\/j.smaim.2025.07.004","article-title":"Nanoparticles in Cancer Therapy: Strategies to Penetrate and Modulate the Tumor Microenvironment \u2013 A Review","volume":"6","author":"Minh Hoang","year":"2025","journal-title":"Smart Mater. Med"},{"issue":"9","key":"2026101904072714600_cit5","doi-asserted-by":"publisher","first-page":"7979","DOI":"10.1021\/acsnano.3c00891","article-title":"Photodynamic and Photothermal Therapies: Synergy Opportunities for Nanomedicine","volume":"17","author":"Overchuk","year":"2023","journal-title":"ACS Nano"},{"key":"2026101904072714600_cit6","doi-asserted-by":"publisher","first-page":"7987","DOI":"10.2147\/IJN.S526497","article-title":"Tumor Microenvironment-Responsive Nanoparticles: Promising Cancer PTT Carriers","volume":"20","author":"Sun","year":"2025","journal-title":"Int. J. Nanomed."},{"issue":"6","key":"2026101904072714600_cit7","doi-asserted-by":"publisher","first-page":"671","DOI":"10.1089\/cbr.2008.0591","article-title":"Turning the Heat on Cancer","volume":"23","author":"DeNardo","year":"2008","journal-title":"Cancer Biother. Radiopharm."},{"key":"2026101904072714600_cit8","doi-asserted-by":"publisher","DOI":"10.1016\/j.colsurfb.2020.111189","article-title":"Molecular-Level Effects on Cell Membrane Models to Explain the Phototoxicity of Gold Shell-Isolated Nanoparticles to Cancer Cells","volume":"194","author":"Camacho","year":"2020","journal-title":"Colloids Surf. B Biointerfaces"},{"key":"2026101904072714600_cit9","doi-asserted-by":"publisher","first-page":"23742","DOI":"10.1021\/acsami.4c02247","article-title":"Enhancing Phototoxicity in Human Colorectal Tumor Cells Through Nanoarchitectonics for Synergistic Photothermal and Photodynamic Therapies","volume":"16","author":"Mendes de Almeida Junior","year":"2024","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"2026101904072714600_cit10","doi-asserted-by":"publisher","DOI":"10.1016\/j.colsurfb.2025.114687","article-title":"Optimizing Near-Infrared-Activated Gold Nanostructures for Targeted Combination Cancer Therapy","volume":"253","author":"Shukla","year":"2025","journal-title":"Colloids Surf. B Biointerfaces"},{"issue":"11","key":"2026101904072714600_cit11","doi-asserted-by":"publisher","first-page":"6891","DOI":"10.1021\/acs.chemrev.3c00159","article-title":"Photothermal Nanomaterials: A Powerful Light-to-Heat Converter","volume":"123","author":"Cui","year":"2023","journal-title":"Chem. Rev."},{"issue":"1","key":"2026101904072714600_cit12","doi-asserted-by":"publisher","DOI":"10.1038\/s42004-025-01705-w","article-title":"Avenues for Integrating Photothermal Therapy in Cancer Clinic","volume":"8","author":"Patnaik","year":"2025","journal-title":"Commun. Chem."},{"key":"2026101904072714600_cit13","doi-asserted-by":"publisher","DOI":"10.1016\/j.pacs.2021.100281","article-title":"Optothermal Properties of Plasmonic Inorganic Nanoparticles for Photoacoustic Applications","volume":"23","author":"Gellini","year":"2021","journal-title":"Photoacoustics"},{"issue":"16","key":"2026101904072714600_cit14","doi-asserted-by":"publisher","first-page":"3214","DOI":"10.1158\/1078-0432.CCR-22-1871","article-title":"Gold Nanostars Obviate Limitations to Laser Interstitial Thermal Therapy (LITT) for the Treatment of Intracranial Tumors","volume":"29","author":"Srinivasan","year":"2023","journal-title":"Clin. Cancer Res."},{"key":"2026101904072714600_cit15","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2025.118399","article-title":"Gold Nanoparticle-Mediated Photothermal Therapy: Expanding the Frontiers of Cancer Treatment and Theragnostics","volume":"190","author":"Amaral","year":"2025","journal-title":"Biomed. Pharmacother."},{"key":"2026101904072714600_cit16","doi-asserted-by":"publisher","DOI":"10.1016\/j.colsurfb.2021.112301","article-title":"The Efficiency of Photothermal Action of Gold Shell-Isolated Nanoparticles against Tumor Cells Depends on Membrane Interactions","volume":"211","author":"Camacho","year":"2022","journal-title":"Colloids Surf. B Biointerfaces"},{"issue":"19","key":"2026101904072714600_cit17","doi-asserted-by":"publisher","first-page":"27816","DOI":"10.1021\/acsami.5c00084","article-title":"Enhancing Photothermal Therapy Against Breast Cancer Cells by Modulating the End Point of Gold Shell-Isolated Nanoparticles Using Nanostraw-Assisted Injection","volume":"17","author":"Camacho","year":"2025","journal-title":"ACS Appl. Mater. Interfaces"},{"issue":"1","key":"2026101904072714600_cit18","doi-asserted-by":"publisher","first-page":"2","DOI":"10.1021\/tx00031a001","article-title":"Mechanisms and Biological Relevance of Lipid Peroxidation Initiation","volume":"6","author":"Dix","year":"1993","journal-title":"Chem. Res. Toxicol."},{"issue":"7","key":"2026101904072714600_cit19","doi-asserted-by":"publisher","first-page":"579","DOI":"10.1038\/nrd2803","article-title":"Targeting Cancer Cells by ROS-Mediated Mechanisms: A Radical Therapeutic Approach?","volume":"8","author":"Trachootham","year":"2009","journal-title":"Nat. Rev. Drug Discov."},{"issue":"44","key":"2026101904072714600_cit20","doi-asserted-by":"publisher","first-page":"44846","DOI":"10.1021\/acsomega.4c08797","article-title":"Gold Nanoparticles for Photothermal and Photodynamic Therapy","volume":"9","author":"Broadbent","year":"2024","journal-title":"ACS Omega"},{"issue":"2","key":"2026101904072714600_cit21","doi-asserted-by":"publisher","first-page":"399","DOI":"10.1039\/D4TB01420K","article-title":"Gold Nanostars and Nanourchins for Enhanced Photothermal Therapy, Bioimaging, and Theranostics","volume":"13","author":"Delgado-Corrales","year":"2025","journal-title":"J. Mater. Chem. B"},{"key":"2026101904072714600_cit22","doi-asserted-by":"publisher","DOI":"10.1016\/j.jphotochemrev.2022.100568","article-title":"A Mini Review of Nanomaterials on Photodynamic Therapy","volume":"54","author":"Dong","year":"2023","journal-title":"J. Photochem. Photobiol., C"},{"issue":"1","key":"2026101904072714600_cit23","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1515\/nanoph-2022-0592","article-title":"Ultrafast Hot Electron Dynamics in Plasmonic Nanostructures: Experiments, Modelling, Design","volume":"12","author":"Schirato","year":"2023","journal-title":"Nanophotonics"},{"issue":"1","key":"2026101904072714600_cit24","doi-asserted-by":"publisher","first-page":"97","DOI":"10.1038\/s42004-023-00895-5","article-title":"Ligand Impact on Reactive Oxygen Species Generation of Au10 and Au25 Nanoclusters upon One- and Two-Photon Excitation","volume":"6","author":"Fakhouri","year":"2023","journal-title":"Commun. Chem."},{"issue":"17","key":"2026101904072714600_cit25","doi-asserted-by":"publisher","first-page":"20811","DOI":"10.1021\/acsami.3c03578","article-title":"Exploring Low-Power Single-Pulsed Laser-Triggered Two-Photon Photodynamic\/Photothermal Combination Therapy Using a Gold Nanostar\/Graphene Quantum Dot Nanohybrid","volume":"15","author":"Soleimany","year":"2023","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"2026101904072714600_cit26","doi-asserted-by":"publisher","DOI":"10.1016\/j.mtbio.2025.102022","article-title":"Engineering Plasmonic Au Nanostars: Enhanced Plasmonic Properties, Preparation and Biomedical Application","volume":"33","author":"Zhang","year":"2025","journal-title":"Mater. Today Bio"},{"issue":"2","key":"2026101904072714600_cit27","doi-asserted-by":"publisher","DOI":"10.3390\/bioengineering6020053","article-title":"Engineered Gold-Based Nanomaterials: Morphologies and Functionalities in Biomedical Applications A Mini Review","volume":"6","author":"Venditti","year":"2019","journal-title":"Bioengineering"},{"key":"2026101904072714600_cit28","doi-asserted-by":"publisher","DOI":"10.1016\/j.apmt.2024.102179","article-title":"Silica Coatings: From Nanostructures to Biological Entities","volume":"38","author":"Crucho","year":"2024","journal-title":"Appl. Mater. Today"},{"issue":"1","key":"2026101904072714600_cit29","doi-asserted-by":"publisher","first-page":"53","DOI":"10.1002\/cnma.201900459","article-title":"Highly Tunable Growth and Etching of Silica Shells on Surfactant-Free Gold Nanostars","volume":"6","author":"Atta","year":"2020","journal-title":"ChemNanoMat"},{"issue":"8","key":"2026101904072714600_cit30","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamem.2023.184216","article-title":"Photochemical Outcomes Triggered by Gold Shell-Isolated Nanorods on Bioinspired Nanoarchitectonics for Bacterial Membranes","volume":"1865","author":"Almeida","year":"2023","journal-title":"Biochim. Biophys. Acta (BBA) - Biomembr."},{"issue":"48","key":"2026101904072714600_cit31","doi-asserted-by":"publisher","first-page":"41748","DOI":"10.1021\/acsami.7b15296","article-title":"In Vivo Computed Tomography\/Photoacoustic Imaging and NIR-Triggered Chemo\u2013Photothermal Combined Therapy Based on a Gold Nanostar-, Mesoporous Silica-, and Thermosensitive Liposome-Composited Nanoprobe","volume":"9","author":"An","year":"2017","journal-title":"ACS Appl. Mater. Interfaces"},{"issue":"37","key":"2026101904072714600_cit32","doi-asserted-by":"publisher","first-page":"20749","DOI":"10.1021\/acs.jpcc.6b01949","article-title":"Shaping Gold Nanostar Electric Fields for Surface-Enhanced Raman Spectroscopy Enhancement via Silica Coating and Selective Etching","volume":"120","author":"Atta","year":"2016","journal-title":"J. Phys. Chem. C"},{"issue":"5","key":"2026101904072714600_cit33","doi-asserted-by":"publisher","first-page":"4285","DOI":"10.1007\/s12274-022-4085-0","article-title":"Indocyanine Green Assembled Free Oxygen-Nanobubbles towards Enhanced near-Infrared Induced Photodynamic Therapy","volume":"15","author":"Yang","year":"2022","journal-title":"Nano Res."},{"issue":"1","key":"2026101904072714600_cit34","doi-asserted-by":"publisher","first-page":"39","DOI":"10.1016\/0022-1759(95)00072-I","article-title":"A Novel Assay for Apoptosis Flow Cytometric Detection of Phosphatidylserine Expression on Early Apoptotic Cells Using Fluorescein Labelled Annexin V","volume":"184","author":"Vermes","year":"1995","journal-title":"J. Immunol. Methods"},{"issue":"3","key":"2026101904072714600_cit35","doi-asserted-by":"publisher","first-page":"223","DOI":"10.4196\/kjpp.2020.24.3.223","article-title":"Sesamin Induces A549 Cell Mitophagy and Mitochondrial Apoptosis via a Reactive Oxygen Species-Mediated Reduction in Mitochondrial Membrane Potential","volume":"24","author":"Yang","year":"2020","journal-title":"Korean J. Physiol. Pharmacol."},{"key":"2026101904072714600_cit36","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1007\/978-1-60761-411-1_4","article-title":"Identification of ROS Using Oxidized DCFDA and Flow-Cytometry","volume-title":"Advanced Protocols in Oxidative Stress II","author":"Eruslanov","year":"2010"},{"issue":"3","key":"2026101904072714600_cit37","doi-asserted-by":"publisher","DOI":"10.1002\/anse.202200005","article-title":"Gold Nanostars: Synthesis, Optical and SERS Analytical Properties","volume":"2","author":"Becerril-Castro","year":"2022","journal-title":"Anal. Sens."},{"issue":"48","key":"2026101904072714600_cit38","doi-asserted-by":"publisher","first-page":"18849","DOI":"10.1021\/jp8054747","article-title":"Gold Nanostars For Surface-Enhanced Raman Scattering: Synthesis, Characterization and Optimization","volume":"112","author":"Khoury","year":"2008","journal-title":"J. Phys. Chem. C"},{"key":"2026101904072714600_cit39","doi-asserted-by":"publisher","first-page":"404","DOI":"10.1016\/j.powtec.2018.12.016","article-title":"A Comprehensive Review on Synthesis, Stability, Thermophysical Properties, and Characterization of Nanofluids","volume":"344","author":"Sezer","year":"2019","journal-title":"Powder Technol."},{"issue":"1","key":"2026101904072714600_cit40","doi-asserted-by":"publisher","first-page":"9502","DOI":"10.1038\/s41598-018-27840-0","article-title":"Determination of the Size Distribution of Non-Spherical Nanoparticles by Electric Birefringence-Based Methods","volume":"8","author":"Arenas-Guerrero","year":"2018","journal-title":"Sci. Rep."},{"key":"2026101904072714600_cit41","doi-asserted-by":"publisher","DOI":"10.3389\/fchem.2020.591325","article-title":"Plasmonic Hot-Electron Reactive Oxygen Species Generation: Fundamentals for Redox Biology","volume":"8","author":"Carrasco","year":"2020","journal-title":"Front. Chem."},{"issue":"3","key":"2026101904072714600_cit42","doi-asserted-by":"publisher","DOI":"10.1002\/nano.202400107","article-title":"Near-Infrared Nanoparticle-Mediated Photothermal Cancer Therapy: A Comprehensive Review of Advances in Monitoring and Controlling Thermal Effects for Effective Cancer Treatment","volume":"6","author":"Oudjedi","year":"2025","journal-title":"Nano Select"},{"key":"2026101904072714600_cit43","doi-asserted-by":"publisher","first-page":"115","DOI":"10.1016\/j.jiec.2022.06.037","article-title":"Fabrication of Mesoporous Silica-Covered Gold Nanostars for Chemophototherapy","volume":"114","author":"Hou","year":"2022","journal-title":"J. Ind. Eng. Chem."},{"key":"2026101904072714600_cit44","doi-asserted-by":"publisher","first-page":"97","DOI":"10.1016\/j.mtcomm.2018.04.012","article-title":"Gold Nanostars Decorated MnO2 Nanosheets for Magnetic Resonance Imaging and Photothermal Erasion of Lung Cancer Cell","volume":"16","author":"Liu","year":"2018","journal-title":"Mater. Today Commun."},{"issue":"24","key":"2026101904072714600_cit45","doi-asserted-by":"publisher","first-page":"1957","DOI":"10.1080\/17435889.2024.2384351","article-title":"The Effects of Conjugating Anti-MUC1 Aptamers on Gold Nanobipyramids and Nanostars for Photothermal Cancer Ablation","volume":"19","author":"Navyatha","year":"2024","journal-title":"Nanomedicine"},{"issue":"3","key":"2026101904072714600_cit46","doi-asserted-by":"publisher","first-page":"1864","DOI":"10.1021\/acs.jpcc.4c06381","article-title":"Plasmonic Nanoparticles for Photothermal Therapy: Benchmarking of Photothermal Properties and Modeling of Heating at Depth in Human Tissues","volume":"129","author":"Skinner","year":"2025","journal-title":"J. Phys. Chem. C"},{"issue":"22","key":"2026101904072714600_cit47","doi-asserted-by":"publisher","DOI":"10.1002\/cphc.202200809","article-title":"Design of Silica@Au Hybrid Nanostars for Enhanced SERS and Photothermal Effect","volume":"24","author":"Kaur","year":"2023","journal-title":"ChemPhysChem"},{"issue":"19","key":"2026101904072714600_cit48","doi-asserted-by":"publisher","first-page":"10647","DOI":"10.1021\/acs.jpcc.6b02005","article-title":"Singlet Oxygen Generation by Laser Irradiation of Gold Nanoparticles","volume":"120","author":"Chadwick","year":"2016","journal-title":"J. Phys. Chem. C"},{"issue":"5","key":"2026101904072714600_cit49","doi-asserted-by":"publisher","first-page":"3100","DOI":"10.1166\/jnn.2010.2165","article-title":"Nanoparticle Platform to Modulate Reaction Mechanism of Phenothiazine Photosensitizers","volume":"10","author":"Tada","year":"2010","journal-title":"J. Nanosci. Nanotechnol."},{"key":"2026101904072714600_cit50","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpc.2023.107077","article-title":"Unveiling the Mechanisms Underlying Photothermal Efficiency of Gold Shell-Isolated Nanoparticles (AuSHINs) on Ductal Mammary Carcinoma Cells (BT-474)","volume":"300","author":"Kobal","year":"2023","journal-title":"Biophys. Chem."},{"issue":"24","key":"2026101904072714600_cit51","doi-asserted-by":"publisher","first-page":"4130","DOI":"10.1002\/smll.201301365","article-title":"Light-Induced Generation of Singlet Oxygen by Naked Gold Nanoparticles and Its Implications to Cancer Cell Phototherapy","volume":"9","author":"Pasparakis","year":"2013","journal-title":"Small"},{"issue":"4","key":"2026101904072714600_cit52","doi-asserted-by":"publisher","first-page":"342","DOI":"10.15386\/mpr-1756","article-title":"A Review of the Current Understanding of Nanoparticles Protein Corona Composition","volume":"93","author":"Breznica","year":"2020","journal-title":"Med. Pharm. Rep."},{"issue":"6","key":"2026101904072714600_cit53","doi-asserted-by":"publisher","first-page":"859","DOI":"10.2217\/17435889.3.6.859","article-title":"Integrated Research Into the Nanoparticle\u2013Protein Corona: A New Focus for Safe, Sustainable and Equitable Development of Nanomedicines","volume":"3","author":"Faunce","year":"2008","journal-title":"Nanomedicine"},{"issue":"1","key":"2026101904072714600_cit54","doi-asserted-by":"publisher","DOI":"10.1186\/s12951-024-02878-7","article-title":"NIR-Activatable Nitric Oxide Generator Based on Nanoparticles Loaded Small-Molecule Photosensitizers for Synergetic Photodynamic\/Gas Therapy","volume":"22","author":"Fu","year":"2024","journal-title":"J. Nanobiotechnol."},{"issue":"2","key":"2026101904072714600_cit55","doi-asserted-by":"publisher","DOI":"10.3390\/pharmaceutics15020432","article-title":"Gold Nanoparticles Induced Size Dependent Cytotoxicity on Human Alveolar Adenocarcinoma Cells by Inhibiting the Ubiquitin Proteasome System","volume":"15","author":"Ibrahim","year":"2023","journal-title":"Pharmaceutics"},{"key":"2026101904072714600_cit56","doi-asserted-by":"publisher","DOI":"10.1016\/j.cis.2022.102746","article-title":"Cetyltrimethylammonium Bromide in the Synthesis of Mesoporous Silica Nanoparticles: General Aspects and in Vitro Toxicity","volume":"307","author":"Carvalho","year":"2022","journal-title":"Adv. Colloid Interface Sci."},{"issue":"1","key":"2026101904072714600_cit57","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-025-24797-9","article-title":"Glucose-Functionalized Gold Nanoparticles for Effective Photothermal Therapy in Lung Cancer","volume":"15","author":"Menendez","year":"2025","journal-title":"Sci. Rep."},{"issue":"5","key":"2026101904072714600_cit58","doi-asserted-by":"publisher","first-page":"1415","DOI":"10.1182\/blood.V84.5.1415.1415","article-title":"Annexin V for Flow Cytometric Detection of Phosphatidylserine Expression on B Cells Undergoing Apoptosis","volume":"84","author":"Koopman","year":"1994","journal-title":"Blood"},{"issue":"1","key":"2026101904072714600_cit59","doi-asserted-by":"publisher","DOI":"10.1186\/s11658-023-00472-7","article-title":"Genetically Encoded Phosphatidylserine Biosensor for in Vitro, Ex Vivo and in Vivo Labelling","volume":"28","author":"Dirvelyte","year":"2023","journal-title":"Cell. Mol. Biol. Lett."},{"issue":"6","key":"2026101904072714600_cit60","doi-asserted-by":"publisher","first-page":"663","DOI":"10.1016\/j.ceb.2004.09.011","article-title":"Death by Design: Apoptosis, Necrosis and Autophagy","volume":"16","author":"Edinger","year":"2004","journal-title":"Curr. Opin. Cell Biol."},{"key":"2026101904072714600_cit61","doi-asserted-by":"publisher","DOI":"10.1016\/j.jphotobiol.2025.113353","article-title":"Membrane-Targeted Photodynamic Mechanisms of Methylene Violet 3RAX in Melanoma Models","volume":"275","author":"de Oliveira","year":"2026","journal-title":"J. Photochem. Photobiol., B"},{"issue":"27","key":"2026101904072714600_cit62","doi-asserted-by":"publisher","first-page":"9818","DOI":"10.1039\/D2NR03408E","article-title":"AIE-Based Gold Nanostar-Berberine Dimer Nanocomposites for PDT and PTT Combination Therapy toward Breast Cancer","volume":"14","author":"Li","year":"2022","journal-title":"Nanoscale"},{"issue":"8","key":"2026101904072714600_cit63","doi-asserted-by":"publisher","first-page":"5322","DOI":"10.1021\/acsabm.0c00651","article-title":"NIR-Triggered Chemo-Photothermal Therapy by Thermosensitive Gold Nanostar@Mesoporous Silica@Liposome-Composited Drug Delivery Systems","volume":"3","author":"Cai","year":"2020","journal-title":"ACS Appl. Bio Mater."},{"issue":"1","key":"2026101904072714600_cit64","doi-asserted-by":"publisher","DOI":"10.1038\/s41378-025-01016-z","article-title":"Nanocomposite Biosensor Tracks Honokiol-Induced Oxidative Stress Dynamics in 3D Hydrogel-Cultured Lung Cancer Cells","volume":"11","author":"Zhu","year":"2025","journal-title":"Microsyst. Nanoeng."},{"issue":"2","key":"2026101904072714600_cit65","doi-asserted-by":"publisher","first-page":"56","DOI":"10.1016\/j.vascn.2013.01.195","article-title":"Troubleshooting the Dichlorofluorescein Assay to Avoid Artifacts in Measurement of Toxicant-Stimulated Cellular Production of Reactive Oxidant Species","volume":"67","author":"Tetz","year":"2013","journal-title":"J. Pharmacol. Toxicol. Methods"}],"container-title":["ACS Applied Bio Materials"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acsabm.6c01440","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/aabmcb\/article-pdf\/doi\/10.1021\/acsabm.6c01440\/68735359\/mt-2026-01440f.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/aabmcb\/article-pdf\/doi\/10.1021\/acsabm.6c01440\/68735359\/mt-2026-01440f.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T08:29:33Z","timestamp":1792398573000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/aabmcb\/article\/9\/20\/9831\/5443898\/Assessment-of-Apoptosis-like-Cell-Death-in-A549"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10,2]]},"references-count":65,"journal-issue":{"issue":"20","published-online":{"date-parts":[[2026,10,2]]},"published-print":{"date-parts":[[2026,10,19]]}},"URL":"https:\/\/doi.org\/10.1021\/acsabm.6c01440","relation":{},"ISSN":["2576-6422"],"issn-type":[{"value":"2576-6422","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10,2]]}}}