{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T20:40:05Z","timestamp":1792528805609,"version":"4.5.0"},"reference-count":51,"publisher":"American Chemical Society (ACS)","license":[{"start":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T00:00:00Z","timestamp":1792454400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T00:00:00Z","timestamp":1792454400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T00:00:00Z","timestamp":1792454400000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["22237003"],"award-info":[{"award-number":["22237003"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Tsinghua-Toyota Joint Research Fund","award":["20253930081"],"award-info":[{"award-number":["20253930081"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Tumor-associated tertiary lymphoid structures (TLSs) can promote anti-tumor immune responses and survival. However, intratumor TLS formation and maturation are limited in tumors with a \u201ccold\u201d immune phenotype, including reduced infiltration of immune cells and a suppressive tumor microenvironment (TME). Here, we present a personalized immunotherapy strategy utilizing a subcutaneously injected, bioengineered hydrogel (scTLS) that acts as an ectopic immune-activation center, effectively decoupled from the suppressive TME. Native immune cells and antigen-presenting cells (APCs) with autologous antigens are recruited through the release of chemokines. An agonist for CD40 is modified on the gel, enabling an immune-activated environment. We demonstrate that scTLSs promote robust dendritic cell (DC) activation and antigen presentation, driving the formation of organized T and B cell zones as confirmed by 3D imaging and transcriptomic analysis. This personalized, autologous vaccine-based approach elicited potent anti-tumor immune responses and effectively inhibited tumor progression across multiple murine models, with enhanced efficacy upon combination with doxorubicin-induced immunogenic cell death and immune checkpoint blockade. By overcoming the spatial and immunosuppressive limitations of the native TME, our scTLS platform offers a versatile, \"off-the-shelf\" engineering strategy for inducing functional TLSs to enhance therapeutic outcomes in a broad spectrum of malignancies.<\/jats:p>","DOI":"10.1021\/acsabm.6c01731","type":"journal-article","created":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T20:01:22Z","timestamp":1792526482000},"source":"Crossref","is-referenced-by-count":0,"title":["Engineering Subcutaneous Ectopic Tertiary Lymphoid Structures as Personalized Immune-Activation Niches for Cancer Immunotherapy"],"prefix":"10.1021","author":[{"given":"Jing-Yun","family":"Su","sequence":"first","affiliation":[{"name":"Tsinghua University , , ,","place":["Beijing, China, 100084"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tian-Yang","family":"Wang","sequence":"additional","affiliation":[{"name":"Tsinghua University , , ,","place":["Beijing, China, 100084"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shao-Hua","family":"Zhuo","sequence":"additional","affiliation":[{"name":"Tsinghua University , , ,","place":["Beijing, China, 100084"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Geng-Hui","family":"Feng","sequence":"additional","affiliation":[{"name":"Tsinghua University , , ,","place":["Beijing, China, 100084"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1215-5010","authenticated-orcid":true,"given":"Yan-Mei","family":"Li","sequence":"additional","affiliation":[{"name":"Tsinghua University , , ,","place":["Beijing, China, 100084"]},{"name":"Tsinghua University , , ,","place":["Beijing, China, 100084"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,10,20]]},"reference":[{"issue":"10","key":"2026102016011839400_cit1","doi-asserted-by":"publisher","first-page":"2188","DOI":"10.1016\/j.immuni.2023.09.011","article-title":"The cancer-immunity cycle: Indication, genotype, and immunotype","volume":"56","author":"Mellman","year":"2023","journal-title":"Immunity"},{"issue":"3","key":"2026102016011839400_cit2","doi-asserted-by":"publisher","first-page":"195","DOI":"10.1038\/s41577-024-01091-9","article-title":"Vax-Innate: improving therapeutic cancer vaccines by modulating T cells and the tumour microenvironment","volume":"25","author":"Baharom","year":"2025","journal-title":"Nat. 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