{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T03:36:11Z","timestamp":1793072171244,"version":"4.5.0"},"reference-count":22,"publisher":"American Chemical Society (ACS)","license":[{"start":{"date-parts":[[2026,10,18]],"date-time":"2026-10-18T00:00:00Z","timestamp":1792281600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,10,18]],"date-time":"2026-10-18T00:00:00Z","timestamp":1792281600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,10,18]],"date-time":"2026-10-18T00:00:00Z","timestamp":1792281600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["22274159"],"award-info":[{"award-number":["22274159"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The transition of metal\u2013organic framework and polymer from random assembly to ordered spatial organization in enzymatic tandem systems is critical for enhancing cascade catalytic performance. Herein, thermo-responsive poly(styrene-co-maleic anhydride)-block-poly(N-isopropylacrylamide) (PSMN) was designed and employed to integrate UiO-66-NH2 (U) with glucose oxidase and horseradish peroxidase (GOx\/HRP), constructing orderly-assembled U@PSMN@GOx\/HRP, random assembled Up-PSMN@GOx\/HRP and PSMN@GOx\/HRP enzyme reactors with spatial confinement. The thermo-responsive conformational transition of flexible PSMN, together with the structural restriction imposed by covalently integrated rigid U, cooperatively established a more ordered spatial confinement space around GOx\/HRP. At 37 \u00b0C, the Kcat value of U@PSMN@GOx\/HRP was 83.2-fold higher than that at 15 \u00b0C, while, 155.5- and 130.1-fold higher than those values of PSMN@GOx\/HRP and UP-PSMN@GOx\/HRP, respectively. The enhanced cascade catalysis was further translated into sensitive glucose detection, affording a broad linear range of 2.0\u20131200.0 \u03bcM and a detection limit of 0.67 \u03bcM, together with reliable analytical performance in orange fruits. This study provides strategy for coupling stimuli-responsive polymer yielded confined space with orderly-assembled MOFs@polymer@enzyme induced confinement to enhance enzymatic cascade catalysis.<\/jats:p>","DOI":"10.1021\/acsabm.6c01883","type":"journal-article","created":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T05:01:55Z","timestamp":1792386115000},"source":"Crossref","is-referenced-by-count":0,"title":["Dual-Mode Spatial Confinement of GOx\/HRP Tandem System in Orderly-Assembled Metal\u2013Organic Framework@Smart Polymer Enzyme Reactors for Glucose Detection"],"prefix":"10.1021","author":[{"given":"Bowen","family":"Zhou","sequence":"first","affiliation":[{"name":"Institute of Chemistry, Chinese Academy of Sciences , , ,","place":["Beijing, P. R. China, 100190"]},{"name":"Beijing Institute of Petrochemical Technology , , ,","place":["Beijing, P. R. China, 102617"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shidi","family":"Zhang","sequence":"additional","affiliation":[{"name":"Institute of Chemistry, Chinese Academy of Sciences , , ,","place":["Beijing, P. R. China, 100190"]},{"name":"Beijing Institute of Petrochemical Technology , , ,","place":["Beijing, P. R. China, 102617"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-0641-5730","authenticated-orcid":true,"given":"Juan","family":"Qiao","sequence":"additional","affiliation":[{"name":"Beijing Institute of Petrochemical Technology , , ,","place":["Beijing, P. R. China, 102617"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8549-7287","authenticated-orcid":true,"given":"Li","family":"Qi","sequence":"additional","affiliation":[{"name":"Institute of Chemistry, Chinese Academy of Sciences , , ,","place":["Beijing, P. R. 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