{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T02:44:36Z","timestamp":1789094676248,"version":"build-2803163510"},"reference-count":54,"publisher":"American Chemical Society (ACS)","issue":"16","license":[{"start":{"date-parts":[[2020,4,2]],"date-time":"2020-04-02T00:00:00Z","timestamp":1585785600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2020,4,2]],"date-time":"2020-04-02T00:00:00Z","timestamp":1585785600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2020,4,2]],"date-time":"2020-04-02T00:00:00Z","timestamp":1585785600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"funder":[{"DOI":"10.13039\/100005825","name":"National Institute of Food and Agriculture","doi-asserted-by":"publisher","award":["2014-67021-21597"],"award-info":[{"award-number":["2014-67021-21597"]}],"id":[{"id":"10.13039\/100005825","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100005825","name":"National Institute of Food and Agriculture","doi-asserted-by":"publisher","award":["2019-67021-29858"],"award-info":[{"award-number":["2019-67021-29858"]}],"id":[{"id":"10.13039\/100005825","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,4,22]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Shiga toxin-producing Escherichia coli (STEC) are critical foodborne pathogens, which cause serious human health issues, including hemolytic uremic syndrome. Illnesses caused by STEC lack effective treatments that target the elimination of these bacteria from the gastrointestinal tract without causing an adverse effect. Reducing this pathogen from a reservoir of STEC is an effective strategy, but the challenges remain due to the lack of efficient, selective antimicrobial agents. We developed specific antibody-conjugated chitosan nanoparticles (CNs) to selectively target and treat STEC in the gastrointestinal tract. Given the great broad-spectrum antimicrobial activity of CN, we conjugated antibodies to CN. Antibodies were raised and purified from egg yolks after immunization of hens with seven different O-side-chain antigens isolated from STEC (O26, O45, O103, O111, O121, O145, and O157). We prepared CN-immunoglobulin Y (IgY) conjugates by forming amide bonds at different ratios of CN:IgY (10:1, 10:2, and 10:4). The CN-IgY conjugated at a 10:2 ratio demonstrated significantly enhanced antimicrobial activity against E. coli O157:H7. Conjugates of CN and anti-STEC IgY antibodies killed corresponding STEC serotypes specifically and selectively, while showing no significant impact on nontargeted bacteria, including Salmonella enterica and Lactobacillus plantarum. The enhanced antimicrobial activity of CN-IgY against STEC was also confirmed in synthetic intestinal fluid, as well as an in vivo animal model of Caenorhabditis elegans. These results suggest that the CN-IgY conjugates have strong and specific antimicrobial activity and that they are also great candidates to eliminate pathogens selectively in the gastrointestinal tract without inhibiting beneficial bacteria.<\/jats:p>","DOI":"10.1021\/acsami.0c02177","type":"journal-article","created":{"date-parts":[[2020,4,2]],"date-time":"2020-04-02T13:32:38Z","timestamp":1585834358000},"page":"18332-18341","source":"Crossref","is-referenced-by-count":29,"title":["Selective\nKilling of Shiga Toxin-Producing\n                    <i>Escherichia coli<\/i>\n                    with Antibody-Conjugated Chitosan Nanoparticles in the Gastrointestinal\nTract"],"prefix":"10.1021","volume":"12","author":[{"given":"Zhengxin","family":"Ma","sequence":"first","affiliation":[{"name":"Emerging Pathogens Institute, University of Florida , 2055 Mowry Road , , ,","place":["Gainesville, Florida, United States, 32611"]},{"name":"University of Florida , , , ,","place":["Gainesville, Florida, United States, 32611"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minyoung","family":"Kang","sequence":"additional","affiliation":[{"name":"Emerging Pathogens Institute, University of Florida , 2055 Mowry Road , , ,","place":["Gainesville, Florida, United States, 32611"]},{"name":"University of Florida , , , ,","place":["Gainesville, Florida, United States, 32611"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shanyu","family":"Meng","sequence":"additional","affiliation":[{"name":"University of Florida , , , ,","place":["Gainesville, Florida, United States, 32611"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0847-5612","authenticated-orcid":true,"given":"Zhaohui","family":"Tong","sequence":"additional","affiliation":[{"name":"University of Florida , , , ,","place":["Gainesville, Florida, United States, 32611"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Soon-Do","family":"Yoon","sequence":"additional","affiliation":[{"name":"Chonnam National University , , , ,","place":["Yeosu, Jeonnam, Republic of Korea, 59626"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yeongseon","family":"Jang","sequence":"additional","affiliation":[{"name":"University of Florida , , , ,","place":["Gainesville, Florida, United\nStates, 32611"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4337-0426","authenticated-orcid":true,"given":"K. Casey","family":"Jeong","sequence":"additional","affiliation":[{"name":"Emerging Pathogens Institute, University of Florida , 2055 Mowry Road , , ,","place":["Gainesville, Florida, United States, 32611"]},{"name":"University of Florida , , , ,","place":["Gainesville, Florida, United States, 32611"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2020,4,2]]},"reference":[{"key":"2026081713195589700_cit1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0001159","article-title":"Incidence and Tracking of Escherichia coli O157:H7 in a Major Produce Production\nRegion in California","volume":"2","author":"Cooley","year":"2007","journal-title":"PLoS One"},{"key":"2026081713195589700_cit2","doi-asserted-by":"publisher","first-page":"1908","DOI":"10.3201\/eid1312.070763","article-title":"Escherichia coli O157: H7 in Feral Swine Near Spinach Fields and Cattle, Central\nCalifornia Coast","volume":"13","author":"Jay","year":"2007","journal-title":"Emerging Infect. Dis."},{"key":"2026081713195589700_cit3","doi-asserted-by":"publisher","first-page":"1771","DOI":"10.1056\/NEJMoa1106483","article-title":"Epidemic Profile of Shiga-toxin-producing Escherichia coli O104:H4 Outbreak in Germany","volume":"365","author":"Frank","year":"2011","journal-title":"N. Engl. J. Med."},{"key":"2026081713195589700_cit4","doi-asserted-by":"publisher","first-page":"26","DOI":"10.1038\/nrmicro2265","article-title":"Molecular Mechanisms of Escherichia\ncoli Pathogenicity","volume":"8","author":"Croxen","year":"2010","journal-title":"Nat. Rev.\nMicrobiol."},{"key":"2026081713195589700_cit5","doi-asserted-by":"publisher","first-page":"123","DOI":"10.1038\/nrmicro818","article-title":"Pathogenic Escherichia coli","volume":"2","author":"Kaper","year":"2004","journal-title":"Nat. Rev.\nMicrobiol."},{"key":"2026081713195589700_cit6","doi-asserted-by":"publisher","first-page":"1721","DOI":"10.4315\/0362-028X-73.9.1721","article-title":"Non-O157 Shiga Toxin-producing Escherichia coli in Foods","volume":"73","author":"Mathusa","year":"2010","journal-title":"J. Food Protect."},{"key":"2026081713195589700_cit7","doi-asserted-by":"publisher","first-page":"7","DOI":"10.3201\/eid1701.P11101","article-title":"Foodborne\nIllness Acquired in The United States-Major Pathogens","volume":"17","author":"Scallan","year":"2011","journal-title":"Emerging Infect. Dis."},{"key":"2026081713195589700_cit8","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.ijfoodmicro.2006.04.039","article-title":"Shiga Toxin-producing Escherichia coli in Ground Beef and Lamb Cuts: Results\nof a one-year study","volume":"111","author":"Barlow","year":"2006","journal-title":"Int. J. Food Microbiol."},{"key":"2026081713195589700_cit9","doi-asserted-by":"publisher","first-page":"2103","DOI":"10.1128\/AEM.02833-10","article-title":"Prevalence\nand Characterization of Non-O157 Shiga Toxin-producing Escherichia coli Isolates from Commercial Ground\nBeef in the United States","volume":"77","author":"Bosilevac","year":"2011","journal-title":"Appl. Environ. Microb."},{"key":"2026081713195589700_cit10","doi-asserted-by":"publisher","first-page":"2224","DOI":"10.4315\/0362-028X-68.10.2224","article-title":"Prevalence of Shiga Toxin-producing Escherichia coli in Beef Cattle","volume":"68","author":"Hussein","year":"2005","journal-title":"J. Food Protect."},{"key":"2026081713195589700_cit11","doi-asserted-by":"publisher","first-page":"217","DOI":"10.1017\/S1466252308001540","article-title":"Probiotics, Prebiotics and Competitive\nExclusion for Prophylaxis against Bacterial Disease","volume":"9","author":"Callaway","year":"2008","journal-title":"Anim. Health Res. Rev."},{"key":"2026081713195589700_cit12","doi-asserted-by":"publisher","first-page":"6210","DOI":"10.1016\/j.vaccine.2012.05.080","article-title":"Efficacy of a Vaccine\nand a Direct-fed Microbial against Fecal Shedding of Escherichia coli O157:H7 in a Randomized Pen-Level\nField Trial of Commercial Feedlot Cattle","volume":"30","author":"Cull","year":"2012","journal-title":"Vaccine"},{"key":"2026081713195589700_cit13","doi-asserted-by":"publisher","first-page":"183","DOI":"10.1089\/fpd.2007.0057","article-title":"Bacteriophage Isolated from Feedlot\nCattle Can Reduce Escherichia coli O157:\nH7 Populations in Ruminant Gastrointestinal Tracts","volume":"5","author":"Callaway","year":"2008","journal-title":"Foodborne Pathog. Dis."},{"key":"2026081713195589700_cit14","doi-asserted-by":"publisher","first-page":"362","DOI":"10.1016\/j.vaccine.2003.08.007","article-title":"Decreased Shedding\nof Escherichia coli O157: H7 by Cattle\nFollowing Vaccination with Type III Secreted Proteins","volume":"22","author":"Potter","year":"2004","journal-title":"Vaccine"},{"key":"2026081713195589700_cit15","doi-asserted-by":"publisher","first-page":"879","DOI":"10.1089\/fpd.2009.0297","article-title":"Escherichia\ncoli O157:H7 Vaccine Dose-Effect in Feedlot Cattle","volume":"6","author":"Moxley","year":"2009","journal-title":"Foodborne Pathog. Dis."},{"key":"2026081713195589700_cit16","doi-asserted-by":"publisher","first-page":"885","DOI":"10.1089\/fpd.2009.0299","article-title":"A Randomized Longitudinal Trial to Test The Effect\nof Regional Vaccination Within A Cattle Feedyard on Escherichia coli O157:H7 Rectal Colonization, Fecal\nShedding, and Hide Contamination","volume":"6","author":"Smith","year":"2009","journal-title":"Foodborne\nPathog. Dis."},{"key":"2026081713195589700_cit17","doi-asserted-by":"publisher","first-page":"186","DOI":"10.1111\/j.1751-7915.2008.00080.x","article-title":"Chitosan and Its\nAntimicrobial Potential - a Critical Literature Survey","volume":"2","author":"Raafat","year":"2009","journal-title":"Microb. Biotechnol."},{"key":"2026081713195589700_cit18","doi-asserted-by":"publisher","first-page":"257","DOI":"10.1016\/j.carbpol.2017.08.082","article-title":"Application, Mode of Action, and in vivo Activity of\nChitosan and Its Micro and Nanoparticles as Antimicrobial Agents:\nA Review","volume":"176","author":"Ma","year":"2017","journal-title":"Carbohydr. Polym."},{"key":"2026081713195589700_cit19","doi-asserted-by":"publisher","first-page":"2693","DOI":"10.1016\/j.carres.2004.09.007","article-title":"Preparation and Antibacterial Activity of Chitosan\nNanoparticles","volume":"339","author":"Qi","year":"2004","journal-title":"Carbohydr. Res."},{"key":"2026081713195589700_cit20","doi-asserted-by":"publisher","first-page":"147","DOI":"10.1016\/j.ijfoodmicro.2004.01.022","article-title":"Chitosan Kills Bacteria Through Cell Membrane Damage","volume":"95","author":"Liu","year":"2004","journal-title":"Int. J. Food Microbiol."},{"key":"2026081713195589700_cit21","doi-asserted-by":"publisher","first-page":"122","DOI":"10.1016\/j.bjp.2015.09.010","article-title":"Evaluation\nof the Antimicrobial Activity of Chitosan and Its Quaternized Derivative\non E. coli and S. aureus Growth","volume":"26","author":"Goy","year":"2016","journal-title":"Rev. Bras. Farmacogn."},{"key":"2026081713195589700_cit22","doi-asserted-by":"publisher","first-page":"5","DOI":"10.1016\/j.jconrel.2004.08.010","article-title":"Recent\nAdvances on Chitosan-Based Micro-and Nanoparticles in Drug Delivery","volume":"100","author":"Agnihotri","year":"2004","journal-title":"J. Controlled Release"},{"key":"2026081713195589700_cit23","doi-asserted-by":"publisher","first-page":"623","DOI":"10.1016\/j.carbpol.2018.06.046","article-title":"Engineering\nof Chitosan-Derived Nanoparticles to Enhance Antimicrobial Activity\nAgainst Foodborne Pathogen Escherichia coli O157:H7","volume":"197","author":"Garrido-Maestu","year":"2018","journal-title":"Carbohydr. Polym."},{"key":"2026081713195589700_cit24","doi-asserted-by":"publisher","first-page":"10700","DOI":"10.1021\/acsami.6b00894","article-title":"Chitosan Microparticles Exert Broad-Spectrum Antimicrobial\nActivity Against Antibiotic-Resistant Micro-Organisms Without Increasing\nResistance","volume":"8","author":"Ma","year":"2016","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"2026081713195589700_cit25","doi-asserted-by":"publisher","first-page":"2611","DOI":"10.1128\/AEM.02587-10","article-title":"Reduction of Escherichia coli O157:H7 Shedding in Cattle by Addition of Chitosan Microparticles\nto Feed","volume":"77","author":"Jeong","year":"2011","journal-title":"Appl. Environ. Microbiol."},{"key":"2026081713195589700_cit26","doi-asserted-by":"publisher","first-page":"640","DOI":"10.1128\/AEM.02856-14","article-title":"Application of Chitosan Microparticles for Reduction\nof Vibrio Species in Seawater and Live Oysters (Crassostrea virginica)","volume":"81","author":"Fang","year":"2015","journal-title":"Appl.\nEnviron. Microbiol."},{"key":"2026081713195589700_cit27","doi-asserted-by":"publisher","first-page":"71","DOI":"10.1016\/j.biomaterials.2016.09.016","article-title":"Application of Chitosan Microparticles\nfor Treatment of Metritis and in vivo Evaluation of Broad Spectrum\nAntimicrobial Activity in Cow Uteri","volume":"110","author":"Jeon","year":"2016","journal-title":"Biomaterials"},{"key":"2026081713195589700_cit28","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0092723","article-title":"Underlying Mechanism of Antimicrobial\nActivity of Chitosan Microparticles and Implications for the Treatment\nof Infectious Diseases","volume":"9","author":"Jeon","year":"2014","journal-title":"PLoS One"},{"key":"2026081713195589700_cit29","doi-asserted-by":"publisher","first-page":"248","DOI":"10.1016\/j.jhazmat.2017.07.071","article-title":"Comprehensive in vitro and in vivo Risk Assessments\nof Chitosan Microparticles Using Human Epithelial Cells and Caenorhabditis elegans","volume":"341","author":"Ma","year":"2018","journal-title":"J.\nHazard. Mater."},{"key":"2026081713195589700_cit30","doi-asserted-by":"publisher","first-page":"249","DOI":"10.1016\/j.anaerobe.2011.11.005","article-title":"Effect of New Antimicrobial Agents on the Ecological\nBalance of Human Microflora","volume":"18","author":"Rashid","year":"2012","journal-title":"Anaerobe"},{"key":"2026081713195589700_cit31","doi-asserted-by":"publisher","first-page":"13780","DOI":"10.1073\/pnas.0706625104","article-title":"Molecular-phylogenetic\nCharacterization of Microbial Community Imbalances in Human Inflammatory\nBowel Diseases","volume":"104","author":"Frank","year":"2007","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"2026081713195589700_cit32","doi-asserted-by":"publisher","first-page":"4639","DOI":"10.1073\/pnas.1001224107","article-title":"Effect of Broad-\nand Narrow-spectrum Antimicrobials on Clostridium difficile and Microbial Diversity in a Model of the Distal Colon","volume":"108","author":"Rea","year":"2011","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"2026081713195589700_cit33","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0095476","article-title":"Short-term\nEffect of Antibiotics on Human Gut Microbiota","volume":"9","author":"Panda","year":"2014","journal-title":"PLoS One"},{"key":"2026081713195589700_cit34","doi-asserted-by":"publisher","first-page":"375","DOI":"10.1007\/978-1-4939-0473-0_31","article-title":"LPS Quantitation\nProcedures","volume":"1149","author":"Lam","year":"2014","journal-title":"Methods Mol Biol"},{"key":"2026081713195589700_cit35","article-title":"Purification and Visualization of\nLipopolysaccharide from Gram-negative Bacteria by Hot Aqueous-phenol\nExtraction","volume":"63","author":"Davis","year":"2012","journal-title":"J. Vis. Exp."},{"key":"2026081713195589700_cit36","first-page":"3","article-title":"Extraction, Purification and Characterization of Lipopolysaccharide\nfrom Escherichia coli and Salmonella typhi","volume":"3","author":"Rezania","year":"2011","journal-title":"Avicenna J. Med. Biotechnol."},{"key":"2026081713195589700_cit37","doi-asserted-by":"publisher","first-page":"569","DOI":"10.1016\/S1095-6433(01)00508-6","article-title":"Generation and Application of Chicken\nEgg-yolk Antibodies","volume":"131","author":"Tini","year":"2002","journal-title":"Comp. Biochem. Physiol.,\nPart A: Mol. Integr. Physiol."},{"key":"2026081713195589700_cit38","doi-asserted-by":"publisher","first-page":"173","DOI":"10.1016\/j.vetimm.2009.12.011","article-title":"IgY: A\nPromising Antibody for Use in Immunodiagnostic and in Immunotherapy","volume":"135","author":"da Silva","year":"2010","journal-title":"Vet. Immunol. Immunopathol."},{"key":"2026081713195589700_cit39","doi-asserted-by":"publisher","first-page":"167","DOI":"10.1111\/j.1472-765X.2011.03085.x","article-title":"Comparison of Four Enrichment Broths for the Detection\nof Non-O157 Shiga-toxin-producing Escherichia coli O91, O103, O111, O119, O121, O145 and O165 from Pure Culture and\nFood Samples","volume":"53","author":"Kanki","year":"2011","journal-title":"Lett. Appl. Microbiol."},{"key":"2026081713195589700_cit40","doi-asserted-by":"publisher","first-page":"124","DOI":"10.1111\/j.1574-6968.2008.01121.x","article-title":"Novel Differential and Confirmation\nPlating Media for Shiga Toxin-producing Escherichia\ncoli serotypes O26, O103, O111, O145 and Sorbitol-positive\nand -negative O157","volume":"282","author":"Posse","year":"2008","journal-title":"FEMS Microbiol. Lett."},{"key":"2026081713195589700_cit41","doi-asserted-by":"publisher","first-page":"192","DOI":"10.4315\/0362-028X.JFP-12-182","article-title":"Chromogenic Agar Medium for Detection\nAnd Isolation of Escherichia coli Serogroups\nO26, O45, O103, O111, O121, and O145 from Fresh Beef and Cattle Feces","volume":"76","author":"Kalchayanand","year":"2013","journal-title":"J. Food Protect."},{"key":"2026081713195589700_cit42","doi-asserted-by":"publisher","first-page":"221","DOI":"10.1111\/j.1472-765X.2007.02286.x","article-title":"Evaluation of Culture, ELISA and PCR Assays for the\nDetection of Salmonella in Seafood","volume":"46","author":"Kumar","year":"2008","journal-title":"Lett. Appl.\nMicrobiol."},{"key":"2026081713195589700_cit43","doi-asserted-by":"publisher","first-page":"101","DOI":"10.4161\/bact.1.2.15666","article-title":"Luminescence Based Enzyme-labeled Phage (Phazyme) Assays\nfor Rapid Detection of Shiga Toxin Producing Escherichia\ncoli Serogroups","volume":"1","author":"Willford","year":"2011","journal-title":"Bacteriophage"},{"key":"2026081713195589700_cit44","doi-asserted-by":"publisher","first-page":"1044","DOI":"10.1089\/fpd.2012.1231","article-title":"Detection of the Top\nSix Non-O157 Shiga Toxin-producing Escherichia coli O Groups by ELISA","volume":"9","author":"Hegde","year":"2012","journal-title":"Foodborne Pathog. Dis."},{"key":"2026081713195589700_cit45","doi-asserted-by":"publisher","first-page":"11403","DOI":"10.1021\/am5019989","article-title":"Fluorescent Nanohybrids Based on Quantum Dot-chitosan-antibody\nas Potential Cancer Biomarkers","volume":"6","author":"Mansur","year":"2014","journal-title":"ACS Appl. Mater.\nInterfaces"},{"key":"2026081713195589700_cit46","volume-title":"Spectrometric Identification of Organic Compounds","author":"Silverstein","year":"1981","edition":"4"},{"key":"2026081713195589700_cit47","doi-asserted-by":"publisher","first-page":"2414","DOI":"10.1128\/IAI.70.5.2414-2418.2002","article-title":"Vaccination of Pregnant Dams with Intimin\u00ad(O157) Protects\nSuckling Piglets from Escherichia coli O157:H7 infection","volume":"70","author":"Dean-Nystrom","year":"2002","journal-title":"Infect. Immun."},{"key":"2026081713195589700_cit48","doi-asserted-by":"publisher","first-page":"439","DOI":"10.1086\/600108","article-title":"Therapeutic\nMonoclonal Antibody Treatment Targeting Respiratory Syncytial Virus\n(RSV) G Protein Mediates Viral Clearance and Reduces the Pathogenesis\nof RSV Infection in BALB\/c Mice","volume":"200","author":"Haynes","year":"2009","journal-title":"J. Infect.\nDis."},{"key":"2026081713195589700_cit49","doi-asserted-by":"publisher","first-page":"3004","DOI":"10.2527\/1994.72113004x","article-title":"Microbial Attachment and Feed Digestion in the Rumen","volume":"72","author":"McAllister","year":"1994","journal-title":"J. Anim. Sci."},{"key":"2026081713195589700_cit50","doi-asserted-by":"publisher","first-page":"687","DOI":"10.1016\/S0142-9612(00)00231-3","article-title":"Chitosan\nmicroparticles for oral vaccination: preparation, characterization\nand preliminary in vivo uptake studies in murine Peyer\u2019s patches","volume":"22","author":"van der Lubben","year":"2001","journal-title":"Biomaterials"},{"key":"2026081713195589700_cit51","doi-asserted-by":"publisher","first-page":"1174","DOI":"10.1021\/bc2006219","article-title":"T cell-specific siRNA Delivery Using Antibody-Conjugated\nChitosan Nanoparticles","volume":"23","author":"Lee","year":"2012","journal-title":"Bioconjugate Chem."},{"key":"2026081713195589700_cit52","doi-asserted-by":"publisher","first-page":"153","DOI":"10.1016\/0168-1605(92)90144-R","article-title":"Campylobacter jejuni Non-culturable Coccoid Cells","volume":"15","author":"Beumer","year":"1992","journal-title":"Int. J. Food\nMicrobiol."},{"key":"2026081713195589700_cit53","doi-asserted-by":"publisher","first-page":"344","DOI":"10.1023\/B:PHAM.0000016249.52831.a5","article-title":"Uptake\nand Cytotoxicity of Chitosan Molecules and Nanoparticles: Effects\nof Molecular Weight and Degree of Deacetylation","volume":"21","author":"Huang","year":"2004","journal-title":"Pharm. Res."},{"key":"2026081713195589700_cit54","doi-asserted-by":"publisher","first-page":"461","DOI":"10.1038\/366461a0","article-title":"A C. elegans mutant that Lives Twice as Long as Wild-type","volume":"366","author":"Kenyon","year":"1993","journal-title":"Nature"}],"container-title":["ACS Applied Materials &amp; Interfaces"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acsami.0c02177","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/aamick\/article-pdf\/12\/16\/18332\/13935311\/am0c02177.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/aamick\/article-pdf\/12\/16\/18332\/13935311\/am0c02177.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T17:23:51Z","timestamp":1786987431000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/aamick\/article\/12\/16\/18332\/583596\/Selective-Killing-of-Shiga-Toxin-Producing"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,4,2]]},"references-count":54,"journal-issue":{"issue":"16","published-online":{"date-parts":[[2020,4,2]]},"published-print":{"date-parts":[[2020,4,22]]}},"URL":"https:\/\/doi.org\/10.1021\/acsami.0c02177","relation":{},"ISSN":["1944-8244","1944-8252"],"issn-type":[{"value":"1944-8244","type":"print"},{"value":"1944-8252","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,4,2]]}}}