{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T21:04:37Z","timestamp":1789506277573,"version":"build-2803163510"},"reference-count":38,"publisher":"American Chemical Society (ACS)","issue":"35","license":[{"start":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T00:00:00Z","timestamp":1787788800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T00:00:00Z","timestamp":1787788800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T00:00:00Z","timestamp":1787788800000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"funder":[{"DOI":"10.13039\/501100018537","name":"National Science and Technology Major Project","doi-asserted-by":"publisher","award":["2024ZD0532700"],"award-info":[{"award-number":["2024ZD0532700"]}],"id":[{"id":"10.13039\/501100018537","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100018540","name":"Science Fund for Distinguished Young Scholars of Guangdong Province","doi-asserted-by":"publisher","award":["2025B1515020071"],"award-info":[{"award-number":["2025B1515020071"]}],"id":[{"id":"10.13039\/501100018540","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,9,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Direct pathogen detection in stool samples is the gold standard for diagnosing gastrointestinal infections, yet substantial technical challenges remain. Here, we report a versatile immunochromatographic assay (ICA) for simultaneous detection of both bacteria and virus, integrating a trimodal readout strategy combining magnetic enrichment with colorimetric, catalytic, and surface-enhanced Raman scattering (SERS) signals. We developed a self-assembled magnetic SERS nanozyme by electrostatically layer-by-layer depositing multilayer PtIr nanoparticles and Au nanostars onto a magnetic core, affording strong peroxidase-like activity and electromagnetic enhancement. In addition, a multi-feature fusion neural-network model was designed for intelligent SERS signal analysis, enabling accurate quantification of pathogen concentrations. The method exhibits high analytical performance across all three modalities: in colorimetric mode, the visual limits of detection (vLODs) were 5 \u00d7 103 CFU\/mL for Escherichia coli O157 and 0.05 ng\/mL for norovirus; in catalytic mode, a 2 min substrate-catalysis step reduced the colorimetric vLOD by 100-fold; in SERS mode, the detection limits were further lowered to 8 CFU\/mL and 0.11 pg\/mL via a quantitative algorithm. Application to 116 clinical stool samples showed detection rates consistent with quantitative real-time PCR, while reducing the assay time to 1\/8. Therefore, this trimodal ICA holds strong potential for rapid and accurate detection of gastrointestinal infections across diverse scenarios.<\/jats:p>","DOI":"10.1021\/acsami.6c07352","type":"journal-article","created":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T18:48:13Z","timestamp":1788288493000},"page":"47308-47325","source":"Crossref","is-referenced-by-count":0,"title":["Colorimetric-Catalytic-SERS Trimodal Immunochromatographic Assay for Multi-Scenario Monitoring of Gastrointestinal Infections"],"prefix":"10.1021","volume":"18","author":[{"given":"Jiaxuan","family":"Li","sequence":"first","affiliation":[{"name":"South China University of Technology , , ,","place":["Guangzhou, China, 510006"]},{"name":"Guangdong Provincial People\u2019s Hospital (Guangdong Academy of Medical Sciences), Southern Medical University , , , ,","place":["Guangzhou, Guangdong, China, 510000"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xingsheng","family":"Yang","sequence":"additional","affiliation":[{"name":"The First Affiliated Hospital of Bengbu Medical University , , ,","place":["Bengbu, China, 233004"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meirou","family":"Lu","sequence":"additional","affiliation":[{"name":"South China University of Technology , , ,","place":["Guangzhou, China, 510006"]},{"name":"Guangdong Provincial People\u2019s Hospital (Guangdong Academy of Medical Sciences), Southern Medical University , , , ,","place":["Guangzhou, Guangdong, China, 510000"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanxiang","family":"Li","sequence":"additional","affiliation":[{"name":"Guangdong Provincial People\u2019s Hospital (Guangdong Academy of Medical Sciences), Southern Medical University , , , ,","place":["Guangzhou, Guangdong, China, 510000"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junyan","family":"Ai","sequence":"additional","affiliation":[{"name":"Guangdong Provincial People\u2019s Hospital (Guangdong Academy of Medical Sciences), Southern Medical University , , , ,","place":["Guangzhou, Guangdong, China, 510000"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-1688-1294","authenticated-orcid":true,"given":"Shuai","family":"Zheng","sequence":"additional","affiliation":[{"name":"Hefei Institute of Physical Science, Chinese Academy of Sciences , ,","place":["Hefei, China, 230036"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9627-107X","authenticated-orcid":true,"given":"Chongwen","family":"Wang","sequence":"additional","affiliation":[{"name":"South China University of Technology , , ,","place":["Guangzhou, China, 510006"]},{"name":"Guangdong Provincial People\u2019s Hospital (Guangdong Academy of Medical Sciences), Southern Medical University , , , ,","place":["Guangzhou, Guangdong, China, 510000"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaocheng","family":"Luo","sequence":"additional","affiliation":[{"name":"Nanning Fourth People's Hospital , , ,","place":["Nanning, China, 530012"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7764-3213","authenticated-orcid":true,"given":"Bing","family":"Gu","sequence":"additional","affiliation":[{"name":"South China University of Technology , , ,","place":["Guangzhou, China, 510006"]},{"name":"Guangdong Provincial People\u2019s Hospital (Guangdong Academy of Medical Sciences), Southern Medical University , , , ,","place":["Guangzhou, Guangdong, China, 510000"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,8,27]]},"reference":[{"issue":"5","key":"2026091516064953000_cit1","doi-asserted-by":"publisher","first-page":"519","DOI":"10.1016\/S1473-3099(24)00691-1","article-title":"Global, regional, and national age-sex-specific burden of diarrhoeal diseases, their risk factors, and aetiologies, 1990\u20132021, for 204 countries and territories: a systematic analysis for the Global Burden of Disease Study 2021","volume":"25","author":"Kyu","year":"1990","journal-title":"Lancet Infect. 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